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The map label for this gene is gfa [H]

Identifier: 146342332

GI number: 146342332

Start: 5669100

End: 5669663

Strand: Direct

Name: gfa [H]

Synonym: BRADO5487

Alternate gene names: 146342332

Gene position: 5669100-5669663 (Clockwise)

Preceding gene: 146342331

Following gene: 146342333

Centisome position: 76.03

GC content: 63.48

Gene sequence:

>564_bases
ATGACTGTCCACATTCATCCGTCCGTCGACCATGGAGTGAAACAAGGGAGCGGCCACTTCGCGGGCGGCACCCTGGTCTG
CAAGTGCCACGATCGGCCGGTGAAGGTCGGCATCAAGGGCGACGTCGCGCACAACCATGCGTGCGGCTGCACCAAATGCT
GGAAGCCGTCGGGCGCAACCTTCTCCGTCGTAGCCGTCGTGCCGCGGCAGAACGTCACGGTGCTCGAGAACGGCGACAAG
TTGAAGATCGTCGATGCCTCGGCCGTGATCCAGCGCTACGCCTGCACGGGCTGCGGCACGCATATGTACGGGCGGATCGA
GAACACGGGTCATCCGTTCTATGGCCTCGACTTCATCCATCCCGAGCTGTTCCAGGAGCAGGGTTCGGCAGCCCCAGGCT
TTGCCGCCTTCGTCTCCTCGGTAATCGAATCGGGCGTGAAGCCGGAGCAGATGGGCGAGATTCGCGCGCGGCTGAAGGAG
CTGGGGCTCGAGCCCTATGACTGCCTGTCGCCGGCGCTGATGGATGCGATTGCCACACATGTGGCGAAAGCAAAGGCGGC
CTGA

Upstream 100 bases:

>100_bases
AGACATCAACAAGGGCTTCGACCTGATGCATGAGGGCAAGTCGATCCGTTCCGTCGTCGTGTTCTGACCCGAGCCACGCA
AAACCGAGGAGGATTGCGCC

Downstream 100 bases:

>100_bases
CGGCCGCGGACTACCAAGCGTAACGACTGAAGCGTTCCTTCCTGAAGACTTGGTGCCTCGCCCGCCTTCGCGCTGGCGAG
GCATTCTTTCTTGGTGGTGC

Product: glutathione-dependent formaldehyde-activating enzyme

Products: NA

Alternate protein names: S-(hydroxymethyl)glutathione synthase [H]

Number of amino acids: Translated: 187; Mature: 186

Protein sequence:

>187_residues
MTVHIHPSVDHGVKQGSGHFAGGTLVCKCHDRPVKVGIKGDVAHNHACGCTKCWKPSGATFSVVAVVPRQNVTVLENGDK
LKIVDASAVIQRYACTGCGTHMYGRIENTGHPFYGLDFIHPELFQEQGSAAPGFAAFVSSVIESGVKPEQMGEIRARLKE
LGLEPYDCLSPALMDAIATHVAKAKAA

Sequences:

>Translated_187_residues
MTVHIHPSVDHGVKQGSGHFAGGTLVCKCHDRPVKVGIKGDVAHNHACGCTKCWKPSGATFSVVAVVPRQNVTVLENGDK
LKIVDASAVIQRYACTGCGTHMYGRIENTGHPFYGLDFIHPELFQEQGSAAPGFAAFVSSVIESGVKPEQMGEIRARLKE
LGLEPYDCLSPALMDAIATHVAKAKAA
>Mature_186_residues
TVHIHPSVDHGVKQGSGHFAGGTLVCKCHDRPVKVGIKGDVAHNHACGCTKCWKPSGATFSVVAVVPRQNVTVLENGDKL
KIVDASAVIQRYACTGCGTHMYGRIENTGHPFYGLDFIHPELFQEQGSAAPGFAAFVSSVIESGVKPEQMGEIRARLKEL
GLEPYDCLSPALMDAIATHVAKAKAA

Specific function: Catalyzes the condensation of formaldehyde and glutathione to S-hydroxymethylglutathione [H]

COG id: COG3791

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gfa family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014185
- InterPro:   IPR006913
- InterPro:   IPR011057 [H]

Pfam domain/function: PF04828 GFA [H]

EC number: =4.4.1.22 [H]

Molecular weight: Translated: 19877; Mature: 19746

Theoretical pI: Translated: 7.81; Mature: 7.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
4.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVHIHPSVDHGVKQGSGHFAGGTLVCKCHDRPVKVGIKGDVAHNHACGCTKCWKPSGAT
CEEEECCCHHHHHCCCCCCCCCCEEEEEECCCEEEEEEECCCCCCCCCCCCCCCCCCCCE
FSVVAVVPRQNVTVLENGDKLKIVDASAVIQRYACTGCGTHMYGRIENTGHPFYGLDFIH
EEEEEEECCCCEEEEECCCEEEEEEHHHHHHHHHHCCCCCHHEEEECCCCCCEECCCCCC
PELFQEQGSAAPGFAAFVSSVIESGVKPEQMGEIRARLKELGLEPYDCLSPALMDAIATH
HHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
VAKAKAA
HHHHCCC
>Mature Secondary Structure 
TVHIHPSVDHGVKQGSGHFAGGTLVCKCHDRPVKVGIKGDVAHNHACGCTKCWKPSGAT
EEEECCCHHHHHCCCCCCCCCCEEEEEECCCEEEEEEECCCCCCCCCCCCCCCCCCCCE
FSVVAVVPRQNVTVLENGDKLKIVDASAVIQRYACTGCGTHMYGRIENTGHPFYGLDFIH
EEEEEEECCCCEEEEECCCEEEEEEHHHHHHHHHHCCCCCHHEEEECCCCCCEECCCCCC
PELFQEQGSAAPGFAAFVSSVIESGVKPEQMGEIRARLKELGLEPYDCLSPALMDAIATH
HHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
VAKAKAA
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA