Click here to switch to the map view.

The map label for this gene is metC [H]

Identifier: 146341756

GI number: 146341756

Start: 5075401

End: 5076696

Strand: Direct

Name: metC [H]

Synonym: BRADO4873

Alternate gene names: 146341756

Gene position: 5075401-5076696 (Clockwise)

Preceding gene: 146341755

Following gene: 146341757

Centisome position: 68.07

GC content: 66.13

Gene sequence:

>1296_bases
ATGCCGCCGCCGAAACCGCCTGCCTTCGAAACCCTGAGCCTGCATGCGGGCCAGCATCCGGATCCCGCGACCGGCGCCCG
CGCGGTGCCGATCTATCAGACCACGTCCTACGTGTTCGACGACACTGAGCACGCCGCCGCTTTGTTCAACCTCGAACGTG
CCGGCCACATCTACACCCGCATCTCCAATCCGACGACCGCCGTGCTCGAAGAGCGGCTCGCCGCGCTGGAAGGCGGTGTC
GGCGCGGTCTGCACCGCGAGCGGCCAGGCCGCGCTGCATCTGGCGATCGCCACGATCCTCAACGCCGGCGACCACATCGT
CGCCTCCTCCTCGCTCTATGGCGGCACCATCAACCTGCTCGCCCACACGCTGCCGCGCTTCGGCATCAACACGAGCTTCG
TGAAGCCGCGCGACCTCGACGGCTTCCGCAGCACCATCAAGCCCAACACGCGGCTCGTGATCGGCGAGACTCTCGGCAAT
CCCGGCCTCGAAGTGCTGGACATCCCGAAAGTGTCCGCGATCGCGCACGAGGCGGGCATCCCGCTCCTGATCGACAACAC
TTTCGCGACGCCCTATTTGAGCCGGCCGATCGCGCAGGGCGCCGACATCGTGATGCATTCGGCGACCAAATGGCTCGGCG
GACATGGTATCGCGATCGGCGGCGTGATCGTCGATGGCGGCCGCTTCGACTGGCGGGCGTCAGGCAAATTCCCTGTGCTC
ACCGAACCCTATGCCGGCTATCACGGCATCGTCTTCGACGAGCAGTTCGGCACCGCCGCCTTCATCATGCGGGCGCGCAC
GGAAGGCTTGCGTGATTTCGGCGCCTGCCTGTCGCCGACCAACGCCTTTCATCTTCTGCAGGGCGTCGAGACCTTGCATC
TGCGCATGGAGCGCCATGTCGCGAACACGGCGGCCGTGCTCGCCGCGCTGACCGCCAACAAGGCCGTCGACTGGGTGCTG
CATCCGTCGCTGGAGACGCATCCCGATCACGCGCTGGCCAAGCAGCTGCTGCCGCGCGGCACCGGCTCGATCGTCTCGTT
CGGCATCAAGGGCGGCCGCGCCGCCGGACGCAAGTTCATCGAGAGCCTGAAGCTGATCAGCCATCTCGCCAATGTCGGCG
ACGCCAAGACGCTGGTGATTCACCCGGCCTCGACGACGCATCAGCAGATGGATGCCGCACAGCTTGCAGCAGCCGGGATC
GGCGAAGAGCTGATCCGGCTGTCGGTAGGCATCGAGGCCGCATCTGATATCATCGATGATCTCGGACAGGCGCTGCGCGC
CTCGCAGAAGGTGTGA

Upstream 100 bases:

>100_bases
GCGTTGGCATGCTGGGGGCGGCGTCATGGCGCCGCCCCTGACGCCCGGTGAAATCTATGGCAGAAAGCAGGACCATCGTC
AGACGAAAGCCGAGACCCGC

Downstream 100 bases:

>100_bases
GCCATGCAGCTGTCGATTGCTGATCGCGATGTGTTCGTGGCGACCGGCGGTCGCGCGTTCGATTCATCCCTGCCCGCTGC
CGTCTTCGTGCACGGCGCCG

Product: O-acetylhomoserine aminocarboxypropyltransferase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MPPPKPPAFETLSLHAGQHPDPATGARAVPIYQTTSYVFDDTEHAAALFNLERAGHIYTRISNPTTAVLEERLAALEGGV
GAVCTASGQAALHLAIATILNAGDHIVASSSLYGGTINLLAHTLPRFGINTSFVKPRDLDGFRSTIKPNTRLVIGETLGN
PGLEVLDIPKVSAIAHEAGIPLLIDNTFATPYLSRPIAQGADIVMHSATKWLGGHGIAIGGVIVDGGRFDWRASGKFPVL
TEPYAGYHGIVFDEQFGTAAFIMRARTEGLRDFGACLSPTNAFHLLQGVETLHLRMERHVANTAAVLAALTANKAVDWVL
HPSLETHPDHALAKQLLPRGTGSIVSFGIKGGRAAGRKFIESLKLISHLANVGDAKTLVIHPASTTHQQMDAAQLAAAGI
GEELIRLSVGIEAASDIIDDLGQALRASQKV

Sequences:

>Translated_431_residues
MPPPKPPAFETLSLHAGQHPDPATGARAVPIYQTTSYVFDDTEHAAALFNLERAGHIYTRISNPTTAVLEERLAALEGGV
GAVCTASGQAALHLAIATILNAGDHIVASSSLYGGTINLLAHTLPRFGINTSFVKPRDLDGFRSTIKPNTRLVIGETLGN
PGLEVLDIPKVSAIAHEAGIPLLIDNTFATPYLSRPIAQGADIVMHSATKWLGGHGIAIGGVIVDGGRFDWRASGKFPVL
TEPYAGYHGIVFDEQFGTAAFIMRARTEGLRDFGACLSPTNAFHLLQGVETLHLRMERHVANTAAVLAALTANKAVDWVL
HPSLETHPDHALAKQLLPRGTGSIVSFGIKGGRAAGRKFIESLKLISHLANVGDAKTLVIHPASTTHQQMDAAQLAAAGI
GEELIRLSVGIEAASDIIDDLGQALRASQKV
>Mature_430_residues
PPPKPPAFETLSLHAGQHPDPATGARAVPIYQTTSYVFDDTEHAAALFNLERAGHIYTRISNPTTAVLEERLAALEGGVG
AVCTASGQAALHLAIATILNAGDHIVASSSLYGGTINLLAHTLPRFGINTSFVKPRDLDGFRSTIKPNTRLVIGETLGNP
GLEVLDIPKVSAIAHEAGIPLLIDNTFATPYLSRPIAQGADIVMHSATKWLGGHGIAIGGVIVDGGRFDWRASGKFPVLT
EPYAGYHGIVFDEQFGTAAFIMRARTEGLRDFGACLSPTNAFHLLQGVETLHLRMERHVANTAAVLAALTANKAVDWVLH
PSLETHPDHALAKQLLPRGTGSIVSFGIKGGRAAGRKFIESLKLISHLANVGDAKTLVIHPASTTHQQMDAAQLAAAGIG
EELIRLSVGIEAASDIIDDLGQALRASQKV

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=423, Percent_Identity=34.2789598108747, Blast_Score=200, Evalue=3e-51,
Organism=Homo sapiens, GI299473759, Length=423, Percent_Identity=32.1513002364066, Blast_Score=165, Evalue=9e-41,
Organism=Homo sapiens, GI25453487, Length=422, Percent_Identity=30.5687203791469, Blast_Score=146, Evalue=3e-35,
Organism=Escherichia coli, GI1790375, Length=420, Percent_Identity=32.8571428571429, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI1789383, Length=424, Percent_Identity=25.9433962264151, Blast_Score=112, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17538055, Length=425, Percent_Identity=34.5882352941176, Blast_Score=216, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI17560056, Length=426, Percent_Identity=34.7417840375587, Blast_Score=211, Evalue=6e-55,
Organism=Caenorhabditis elegans, GI71980708, Length=429, Percent_Identity=31.9347319347319, Blast_Score=203, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6323333, Length=435, Percent_Identity=48.0459770114943, Blast_Score=364, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6319307, Length=427, Percent_Identity=33.7236533957845, Blast_Score=180, Evalue=4e-46,
Organism=Saccharomyces cerevisiae, GI6321170, Length=355, Percent_Identity=30.7042253521127, Blast_Score=137, Evalue=5e-33,
Organism=Saccharomyces cerevisiae, GI6321254, Length=447, Percent_Identity=26.6219239373602, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6321904, Length=429, Percent_Identity=25.8741258741259, Blast_Score=91, Evalue=4e-19,
Organism=Drosophila melanogaster, GI19922554, Length=431, Percent_Identity=34.1067285382831, Blast_Score=223, Evalue=2e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 45537; Mature: 45405

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPPKPPAFETLSLHAGQHPDPATGARAVPIYQTTSYVFDDTEHAAALFNLERAGHIYTR
CCCCCCCCCCEEEECCCCCCCCCCCCEEEEEEECCCEEECCCHHHHHHHHHHHCCEEEEE
ISNPTTAVLEERLAALEGGVGAVCTASGQAALHLAIATILNAGDHIVASSSLYGGTINLL
ECCCHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCCCCEEECCCCCCCHHHHH
AHTLPRFGINTSFVKPRDLDGFRSTIKPNTRLVIGETLGNPGLEVLDIPKVSAIAHEAGI
HHHHHHCCCCCCCCCCCCCCHHHHHCCCCCEEEEEECCCCCCCEEEECCCHHHHHHHCCC
PLLIDNTFATPYLSRPIAQGADIVMHSATKWLGGHGIAIGGVIVDGGRFDWRASGKFPVL
CEEEECCCCCCHHHCHHHCCCHHHHHHHHHHCCCCCEEEEEEEEECCCEEEECCCCCCEE
TEPYAGYHGIVFDEQFGTAAFIMRARTEGLRDFGACLSPTNAFHLLQGVETLHLRMERHV
ECCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
ANTAAVLAALTANKAVDWVLHPSLETHPDHALAKQLLPRGTGSIVSFGIKGGRAAGRKFI
HHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHCCCCCCCEEEECCCCCHHHHHHHH
ESLKLISHLANVGDAKTLVIHPASTTHQQMDAAQLAAAGIGEELIRLSVGIEAASDIIDD
HHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHHH
LGQALRASQKV
HHHHHHHHCCC
>Mature Secondary Structure 
PPPKPPAFETLSLHAGQHPDPATGARAVPIYQTTSYVFDDTEHAAALFNLERAGHIYTR
CCCCCCCCCEEEECCCCCCCCCCCCEEEEEEECCCEEECCCHHHHHHHHHHHCCEEEEE
ISNPTTAVLEERLAALEGGVGAVCTASGQAALHLAIATILNAGDHIVASSSLYGGTINLL
ECCCHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCCCCEEECCCCCCCHHHHH
AHTLPRFGINTSFVKPRDLDGFRSTIKPNTRLVIGETLGNPGLEVLDIPKVSAIAHEAGI
HHHHHHCCCCCCCCCCCCCCHHHHHCCCCCEEEEEECCCCCCCEEEECCCHHHHHHHCCC
PLLIDNTFATPYLSRPIAQGADIVMHSATKWLGGHGIAIGGVIVDGGRFDWRASGKFPVL
CEEEECCCCCCHHHCHHHCCCHHHHHHHHHHCCCCCEEEEEEEEECCCEEEECCCCCCEE
TEPYAGYHGIVFDEQFGTAAFIMRARTEGLRDFGACLSPTNAFHLLQGVETLHLRMERHV
ECCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
ANTAAVLAALTANKAVDWVLHPSLETHPDHALAKQLLPRGTGSIVSFGIKGGRAAGRKFI
HHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHCCCCCCCEEEECCCCCHHHHHHHH
ESLKLISHLANVGDAKTLVIHPASTTHQQMDAAQLAAAGIGEELIRLSVGIEAASDIIDD
HHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHHH
LGQALRASQKV
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]