| Definition | Bradyrhizobium sp. ORS278 chromosome, complete genome. |
|---|---|
| Accession | NC_009445 |
| Length | 7,456,587 |
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The map label for this gene is nadE [H]
Identifier: 146341601
GI number: 146341601
Start: 4892767
End: 4894095
Strand: Reverse
Name: nadE [H]
Synonym: BRADO4702
Alternate gene names: 146341601
Gene position: 4894095-4892767 (Counterclockwise)
Preceding gene: 146341602
Following gene: 146341598
Centisome position: 65.63
GC content: 71.03
Gene sequence:
>1329_bases ATGGCCGCTGACAGCTTCAAGATCACGCTGGCCCAGCTCAATCCGACGGCGGGGGACATCGCCGGCAACGCCGCGAAGGT GCGCGAGGCCCGGGCGAAGGCCGCCACCGACGGCGCCGACCTCGTGGTGCTGCCGGAGCTGTTCCTGTCAGGCGGCCTGG CCTACGATGTCGAGCCGTTGTGCCGCGCGGCGATCGAGGCGCTGGCGCACGAGACCGCCGATAGCGGGCCGGCGGTGCTG ATCGGCACGCCCTGGGCGGACGACGGCAAGCTCCATGATGCGATGGCGCTGCTCGATGCCGGCCGCATCGCTGCCACACG CTTCAAGATCAATCTCGCGGACGGCCATCAGCAGCGCCTGTTCACGCGCGGCCCCGCCGCCGGCCCGGTCAGCGTCCGCG GTGTGCGCATCGGCATCGCGATCGGCGAGGACATCTGGCCGGAGGCTTTGGGCGGCGAGAACGTCGTCGAAACCCTGGCG GAGACCGGCGCGGAGCTGATCCTGGTGGCCGCGGCGGCGCGTTACGTCCTTGGCGAGGGAGATCGGCGCCTGTCGGCCGC CGTGGCCCGCGTCACCGAGAGCGACCTGCCGCTGATCTGGCTCAATCTGACCGGCGGGCAGGGGGACGACGTGTTCGACG GCGCGTCCTTCGCGCTCAACGCCGACCTCTCGATGGCCGTGCAACTGCCGGCCTTCGTCGACGATGTCAGCACGCTGGCG TGGCGCAGGGGCAATGACGGCTGGCAATGCCGCGGACCGGTCAGCCCATTGATCGACGGCGAAGAGGCTGACTACGCGGC CCGTGTGCTCGGCCTGCGCGACCGTGTCGCCAAGGGCGGCCTCACTGGCGTCGTGCTGGAGCTGACGGCGGATGTCGAAG CGGCGCTGTCGTTGGCGATCGCGGTCGATGCGCTCGGGCCCGCGCAGGTGCGCGCGGTGATGCTGTCCGGACCGGACACG CAGGCCCCGGACGAGGCGATCGCGGCCGCCACGCGGCTCGGCGTCGCCGCCGAGGTGGTGCCGATCGCGGCGGCCGTCGC GGGCTTCGAAAACATCCTCGCCGGACCGCTGTCGCACAGCGCCCATGACGATCTGCTGACGAGCGTGCGCGCGACCCTGT TGAAGGCGATGGCAAGCCTCTCCGGTGCGCTCGCCTGTCGTCCGCTCCAAGGATCGGGTCGGCAGACGTGCGATGTCACA TCGGGAGAGCTGATTCGCCTCGCAGCGTTGCGCAACCGGTGGAAGCCCGTCGGCGCATTGGGCGCCTCGGGCGAGGTGAT CCCGCCTGATCTCATCGACCGCGCGCCGCGCGATGCGGACCAAGGATGA
Upstream 100 bases:
>100_bases TCTCGGCTCCGCCGCGGTCGGCGTCGCGCTCTTGATGTCCGGCAGCTTCTGGCTGTTCGCGATCGCCGAGCGGCTCGGAT TGCTGTGAATGAGTTGAGCG
Downstream 100 bases:
>100_bases CATCGATGGCCCGGCGAACTCGCTCAGCGCGGCGGGATGAAGGTCGGGCCGACCGTGCGGATCTGCTTGTCGGCACCGGC GCCGGTCGTGGTGTCGGCGG
Product: putative glutamine-dependent NAD(+) synthetase (NadE-like)
Products: NA
Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]
Number of amino acids: Translated: 442; Mature: 441
Protein sequence:
>442_residues MAADSFKITLAQLNPTAGDIAGNAAKVREARAKAATDGADLVVLPELFLSGGLAYDVEPLCRAAIEALAHETADSGPAVL IGTPWADDGKLHDAMALLDAGRIAATRFKINLADGHQQRLFTRGPAAGPVSVRGVRIGIAIGEDIWPEALGGENVVETLA ETGAELILVAAAARYVLGEGDRRLSAAVARVTESDLPLIWLNLTGGQGDDVFDGASFALNADLSMAVQLPAFVDDVSTLA WRRGNDGWQCRGPVSPLIDGEEADYAARVLGLRDRVAKGGLTGVVLELTADVEAALSLAIAVDALGPAQVRAVMLSGPDT QAPDEAIAAATRLGVAAEVVPIAAAVAGFENILAGPLSHSAHDDLLTSVRATLLKAMASLSGALACRPLQGSGRQTCDVT SGELIRLAALRNRWKPVGALGASGEVIPPDLIDRAPRDADQG
Sequences:
>Translated_442_residues MAADSFKITLAQLNPTAGDIAGNAAKVREARAKAATDGADLVVLPELFLSGGLAYDVEPLCRAAIEALAHETADSGPAVL IGTPWADDGKLHDAMALLDAGRIAATRFKINLADGHQQRLFTRGPAAGPVSVRGVRIGIAIGEDIWPEALGGENVVETLA ETGAELILVAAAARYVLGEGDRRLSAAVARVTESDLPLIWLNLTGGQGDDVFDGASFALNADLSMAVQLPAFVDDVSTLA WRRGNDGWQCRGPVSPLIDGEEADYAARVLGLRDRVAKGGLTGVVLELTADVEAALSLAIAVDALGPAQVRAVMLSGPDT QAPDEAIAAATRLGVAAEVVPIAAAVAGFENILAGPLSHSAHDDLLTSVRATLLKAMASLSGALACRPLQGSGRQTCDVT SGELIRLAALRNRWKPVGALGASGEVIPPDLIDRAPRDADQG >Mature_441_residues AADSFKITLAQLNPTAGDIAGNAAKVREARAKAATDGADLVVLPELFLSGGLAYDVEPLCRAAIEALAHETADSGPAVLI GTPWADDGKLHDAMALLDAGRIAATRFKINLADGHQQRLFTRGPAAGPVSVRGVRIGIAIGEDIWPEALGGENVVETLAE TGAELILVAAAARYVLGEGDRRLSAAVARVTESDLPLIWLNLTGGQGDDVFDGASFALNADLSMAVQLPAFVDDVSTLAW RRGNDGWQCRGPVSPLIDGEEADYAARVLGLRDRVAKGGLTGVVLELTADVEAALSLAIAVDALGPAQVRAVMLSGPDTQ APDEAIAAATRLGVAAEVVPIAAAVAGFENILAGPLSHSAHDDLLTSVRATLLKAMASLSGALACRPLQGSGRQTCDVTS GELIRLAALRNRWKPVGALGASGEVIPPDLIDRAPRDADQG
Specific function: It is not known if this NAD synthase uses NH(3) or glutamine as the N donor [H]
COG id: COG0388
COG function: function code R; Predicted amidohydrolase
Gene ontology:
Cell location: Cytoplasmic
Metaboloic importance: NA
Operon status: Not Known
Operon components: None
Similarity: Contains 1 CN hydrolase domain [H]
Homologues:
None
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR014445 - InterPro: IPR022310 - InterPro: IPR003694 - InterPro: IPR003010 - InterPro: IPR014729 [H]
Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]
EC number: =6.3.5.1 [H]
Molecular weight: Translated: 45485; Mature: 45354
Theoretical pI: Translated: 4.43; Mature: 4.43
Prosite motif: PS50263 CN_HYDROLASE
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.9 %Cys (Translated Protein) 1.1 %Met (Translated Protein) 2.0 %Cys+Met (Translated Protein) 0.9 %Cys (Mature Protein) 0.9 %Met (Mature Protein) 1.8 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MAADSFKITLAQLNPTAGDIAGNAAKVREARAKAATDGADLVVLPELFLSGGLAYDVEPL CCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEHHHHHCCCCCCCCCHH CRAAIEALAHETADSGPAVLIGTPWADDGKLHDAMALLDAGRIAATRFKINLADGHQQRL HHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHCCCEEEEEEEEEECCCCHHHH FTRGPAAGPVSVRGVRIGIAIGEDIWPEALGGENVVETLAETGAELILVAAAARYVLGEG EECCCCCCCEEEEEEEEEEEECCCCCCHHCCCHHHHHHHHHCCHHEEHHHHHHHHHHCCC DRRLSAAVARVTESDLPLIWLNLTGGQGDDVFDGASFALNADLSMAVQLPAFVDDVSTLA CHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCEEEECCCEEEEECCHHHHHHHHHH WRRGNDGWQCRGPVSPLIDGEEADYAARVLGLRDRVAKGGLTGVVLELTADVEAALSLAI HHCCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHH AVDALGPAQVRAVMLSGPDTQAPDEAIAAATRLGVAAEVVPIAAAVAGFENILAGPLSHS HEECCCCCCEEEEEEECCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCC AHDDLLTSVRATLLKAMASLSGALACRPLQGSGRQTCDVTSGELIRLAALRNRWKPVGAL HHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEECCCCHHHHHHHHHHCCCCCCCC GASGEVIPPDLIDRAPRDADQG CCCCCCCCHHHHHCCCCCCCCC >Mature Secondary Structure AADSFKITLAQLNPTAGDIAGNAAKVREARAKAATDGADLVVLPELFLSGGLAYDVEPL CCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEHHHHHCCCCCCCCCHH CRAAIEALAHETADSGPAVLIGTPWADDGKLHDAMALLDAGRIAATRFKINLADGHQQRL HHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHCCCEEEEEEEEEECCCCHHHH FTRGPAAGPVSVRGVRIGIAIGEDIWPEALGGENVVETLAETGAELILVAAAARYVLGEG EECCCCCCCEEEEEEEEEEEECCCCCCHHCCCHHHHHHHHHCCHHEEHHHHHHHHHHCCC DRRLSAAVARVTESDLPLIWLNLTGGQGDDVFDGASFALNADLSMAVQLPAFVDDVSTLA CHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCEEEECCCEEEEECCHHHHHHHHHH WRRGNDGWQCRGPVSPLIDGEEADYAARVLGLRDRVAKGGLTGVVLELTADVEAALSLAI HHCCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHH AVDALGPAQVRAVMLSGPDTQAPDEAIAAATRLGVAAEVVPIAAAVAGFENILAGPLSHS HEECCCCCCEEEEEEECCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCC AHDDLLTSVRATLLKAMASLSGALACRPLQGSGRQTCDVTSGELIRLAALRNRWKPVGAL HHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEECCCCHHHHHHHHHHCCCCCCCC GASGEVIPPDLIDRAPRDADQG CCCCCCCCHHHHHCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 8431308; 8195100 [H]