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The map label for this gene is potF [H]

Identifier: 146341580

GI number: 146341580

Start: 4869602

End: 4870702

Strand: Reverse

Name: potF [H]

Synonym: BRADO4681

Alternate gene names: 146341580

Gene position: 4870702-4869602 (Counterclockwise)

Preceding gene: 146341581

Following gene: 146341572

Centisome position: 65.32

GC content: 61.76

Gene sequence:

>1101_bases
ATGACGCGCGGCTGCATTCGCTCGGCTCTGGCCCTGGCGGCCGCAGTACTGACGCTCTCAGCTGCGGCAGCTGAGGAGCG
CGTCGTGAACTTCTACAACTGGTCGAACTACATGGCGCCTGGTGTCCTTGAGGATTTCACCAAGGAGACCGGCATCAAGG
TCGTCTACGATACCTTCGACGCCAACGAGACGCTGGAGACAAGGCTGCTCGCCGGCAAGTCCGGCTACGACGTCGTGGTG
CCCACGGCGTATTTCCTCCAGCGCCAGATCAAGGCCAAGATCTTCCAGCCGCTCGACAAGGCGAAGCTGCCGAACCTCGC
CAATGCCTGGACGGTAGTCACCGCGCGTCTCGCCACCTACGATCCCGGCAACCAGTTCGCCGCCAACTACATGTGGGGCA
CGACCGGCATCGGCTACAATGTGAAGGCAATCGAGAAGATCCTGGGCGCGGGCGTGCGGATCGACAGCTGGGACATGGTG
TTCAAGCCGGAGAACCTTGCCAGGTTCAAGGATTGCGGCGTCCATATGCTGGATTCCGCCGACGACATCTTCCCGGCGGC
GCTGAACTATCTCGGCCTCGATCCCAACTCGACCAAGCAGGCTGACCTGGAGAAGGCGGCCGAAGTGGTCGGCAAGGTCG
CGCCCTTCGTGCGCAAGTTTCATTCGTCGGAATATCTGAGCGCGCTCGCCACCGGTGAGATCTGCCTCGTTGTCGGCTGG
TCCGGCGACATCATGCAGGCCCGCAGCCGCGCAGAGGAGGCCAAGAACGGCATCGAGATCGGCTACGCGATCCCGAAAGA
GGGCGCGCAGATGTTCTTCGACAATTTTGCCATCCCAGCCGATGCCCAGCACGTGCCCGAGGCGTATGCGCTGATCAACT
ATCTCTACCGGCCTGAAGTCGCGGCCAAGAACTCGGACTTCCTGTCCTACGCCAACGGCAATCTGGCGAGCCAGAAGCTG
ATCGATCCGAAGATCCTCGGTGATAAGAACATATATCCCGATGACGCGACCCTGAAGAAGCTGTTCGTCATCGCCGCCCG
CGAGCCGGCGACGCAGCGCCTGATCAACCGGCTCTGGACGAAGGTGAAGACGGGGCGGTAG

Upstream 100 bases:

>100_bases
CTCGGTCGTCCGCTTCTGCTTCGCCAAGAAGGACGCCACGCTCGACACGGCGCTGGAGCGCCTGTCCGATGCCATCCACC
GACGCTAGGAGATGAAGGAC

Downstream 100 bases:

>100_bases
TTCGCGCTGTCGTCATTGCGAAGCAATCCAGGGCGAAGACGACGACTCTGGATTGCTTCGTCGCGTTGCTCCTCGCAATG
ACGCCGGAGAGATCAGGCCA

Product: putrescine ABC transporter periplasmic binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 365

Protein sequence:

>366_residues
MTRGCIRSALALAAAVLTLSAAAAEERVVNFYNWSNYMAPGVLEDFTKETGIKVVYDTFDANETLETRLLAGKSGYDVVV
PTAYFLQRQIKAKIFQPLDKAKLPNLANAWTVVTARLATYDPGNQFAANYMWGTTGIGYNVKAIEKILGAGVRIDSWDMV
FKPENLARFKDCGVHMLDSADDIFPAALNYLGLDPNSTKQADLEKAAEVVGKVAPFVRKFHSSEYLSALATGEICLVVGW
SGDIMQARSRAEEAKNGIEIGYAIPKEGAQMFFDNFAIPADAQHVPEAYALINYLYRPEVAAKNSDFLSYANGNLASQKL
IDPKILGDKNIYPDDATLKKLFVIAAREPATQRLINRLWTKVKTGR

Sequences:

>Translated_366_residues
MTRGCIRSALALAAAVLTLSAAAAEERVVNFYNWSNYMAPGVLEDFTKETGIKVVYDTFDANETLETRLLAGKSGYDVVV
PTAYFLQRQIKAKIFQPLDKAKLPNLANAWTVVTARLATYDPGNQFAANYMWGTTGIGYNVKAIEKILGAGVRIDSWDMV
FKPENLARFKDCGVHMLDSADDIFPAALNYLGLDPNSTKQADLEKAAEVVGKVAPFVRKFHSSEYLSALATGEICLVVGW
SGDIMQARSRAEEAKNGIEIGYAIPKEGAQMFFDNFAIPADAQHVPEAYALINYLYRPEVAAKNSDFLSYANGNLASQKL
IDPKILGDKNIYPDDATLKKLFVIAAREPATQRLINRLWTKVKTGR
>Mature_365_residues
TRGCIRSALALAAAVLTLSAAAAEERVVNFYNWSNYMAPGVLEDFTKETGIKVVYDTFDANETLETRLLAGKSGYDVVVP
TAYFLQRQIKAKIFQPLDKAKLPNLANAWTVVTARLATYDPGNQFAANYMWGTTGIGYNVKAIEKILGAGVRIDSWDMVF
KPENLARFKDCGVHMLDSADDIFPAALNYLGLDPNSTKQADLEKAAEVVGKVAPFVRKFHSSEYLSALATGEICLVVGWS
GDIMQARSRAEEAKNGIEIGYAIPKEGAQMFFDNFAIPADAQHVPEAYALINYLYRPEVAAKNSDFLSYANGNLASQKLI
DPKILGDKNIYPDDATLKKLFVIAAREPATQRLINRLWTKVKTGR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=364, Percent_Identity=49.4505494505495, Blast_Score=379, Evalue=1e-106,
Organism=Escherichia coli, GI1787367, Length=323, Percent_Identity=36.8421052631579, Blast_Score=201, Evalue=7e-53,
Organism=Escherichia coli, GI1787711, Length=331, Percent_Identity=24.773413897281, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40299; Mature: 40168

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRGCIRSALALAAAVLTLSAAAAEERVVNFYNWSNYMAPGVLEDFTKETGIKVVYDTFD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCEEEEECCC
ANETLETRLLAGKSGYDVVVPTAYFLQRQIKAKIFQPLDKAKLPNLANAWTVVTARLATY
CCCHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHCC
DPGNQFAANYMWGTTGIGYNVKAIEKILGAGVRIDSWDMVFKPENLARFKDCGVHMLDSA
CCCCCHHHHEEECCCCCCCCHHHHHHHHCCCCEECCCCEEECCCHHHHHHHCCCEEECCC
DDIFPAALNYLGLDPNSTKQADLEKAAEVVGKVAPFVRKFHSSEYLSALATGEICLVVGW
CHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEEEC
SGDIMQARSRAEEAKNGIEIGYAIPKEGAQMFFDNFAIPADAQHVPEAYALINYLYRPEV
CCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCC
AAKNSDFLSYANGNLASQKLIDPKILGDKNIYPDDATLKKLFVIAAREPATQRLINRLWT
CCCCCCCEEECCCCCCHHHCCCCCCCCCCCCCCCCHHHHHEEEEECCCHHHHHHHHHHHH
KVKTGR
HHCCCC
>Mature Secondary Structure 
TRGCIRSALALAAAVLTLSAAAAEERVVNFYNWSNYMAPGVLEDFTKETGIKVVYDTFD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCEEEEECCC
ANETLETRLLAGKSGYDVVVPTAYFLQRQIKAKIFQPLDKAKLPNLANAWTVVTARLATY
CCCHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHCC
DPGNQFAANYMWGTTGIGYNVKAIEKILGAGVRIDSWDMVFKPENLARFKDCGVHMLDSA
CCCCCHHHHEEECCCCCCCCHHHHHHHHCCCCEECCCCEEECCCHHHHHHHCCCEEECCC
DDIFPAALNYLGLDPNSTKQADLEKAAEVVGKVAPFVRKFHSSEYLSALATGEICLVVGW
CHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEEEC
SGDIMQARSRAEEAKNGIEIGYAIPKEGAQMFFDNFAIPADAQHVPEAYALINYLYRPEV
CCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCC
AAKNSDFLSYANGNLASQKLIDPKILGDKNIYPDDATLKKLFVIAAREPATQRLINRLWT
CCCCCCCEEECCCCCCHHHCCCCCCCCCCCCCCCCHHHHHEEEEECCCHHHHHHHHHHHH
KVKTGR
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]