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The map label for this gene is 146341477

Identifier: 146341477

GI number: 146341477

Start: 4766408

End: 4767904

Strand: Reverse

Name: 146341477

Synonym: BRADO4570

Alternate gene names: NA

Gene position: 4767904-4766408 (Counterclockwise)

Preceding gene: 146341481

Following gene: 146341476

Centisome position: 63.94

GC content: 70.41

Gene sequence:

>1497_bases
GTGACGGCCCTCTGCGATCAAACTGCCAATGAGCTGGTCAAGCTCCTGAAATTCAAGGCGATCTCGCCAGTCGCGCTGCT
CGATTCGTGCCTGGAGCGGATCGGCGCGGTCAATCCGGCGGTCAACGCGGTGGTGACCTTGGACGAGCCGGGAGCGCGGG
CGGCGGCCCGGCGCGCCGAGTCGGAGCTTCTCCGCGGCGAGGACAAGGGTCCGCTGCACGGCCTGCCGGTGCTGATCAAG
GACACCCAGGACACCGCCGGGATGCGCTCGACCTATGGCAGCCCGATCCTGGCCGGCAACATTCCGGCGATCGACCAGGC
GTCGGTGGCGCGGCTGCGCGCGGCCGGCGCGATCATCTTCGGAAAGACCAACACGCCGGAATGGGCGGCCGGCGGCAATA
CCCGCAATCCCGTGTTCGGCGCCACCGGCAATCCGTTCGACACGCTGCGCTCGGCGGCCGGCTCCTCCGGCGGCTCGGCG
GCGGCGCTGGCCTGCGGCATGGCGCCGCTCGCCTCGGGCTCGGACACCGGCGGCTCCTTGCGCAATCCGGCCGGCTTCTG
CGGCATCGTCGGTTTCAGGCCGTCCTATGGGCTCGTCGCGAGCGAGAAGCGCGCGCATGGCTGGTCGTGCCTGTCGACCG
ACGGGCCGATGGCGCGCGACGTCGCCGACACCGCGCTGATGCTGTCGGTGATGGCAAGCGACGATGTGCGCGATCCGCTC
GCCTATACGCTGCCGGGCGAAGCGGTGCGCGGACAGCCTGCGCGCTGGGCCGTGCCGCCGCAGGTGGAGTTGCGCAAGCT
CAGGCTCGCCTTCACCGAGGATTTTAATTTCGCGCCGACCGAGCAACTGATCCGCCGCGCCTTTCGCGCCCGCGTGTCGC
GCATCGCGCCGCTGTTTGCCGAGGCCGCCGAGGCCGCGCCGGATTGCAGCGGCGCCGACGAGACCTTCGCGGTGCTGCGC
GCGTCGCTGTTCCTCACCCAGCACGGCAAGAACTATCGCGAGCGGCCCGAGATGATCGGGCCGAACGTGCGCGCCAATGT
CGAGGAGGGGCTGTCCTACAGCCTCGCGGATTTTTCGCGCGCCGCGGCCAACCAGACCCGGATCTATCGCGCCTATCAGA
GTTTCTTCGCGAAGCACGACGTGCTGATCAGTCCGACCATCACGCTGAGCCCGCGGCCCTGGTCGGAGCTGTATCCGGCC
GAGATCGACGGCGCGCCGACCAAGTCCTACTTCCACTGGCTGGCGCTGGCCTATGCGGTGACGCTCGCGGGGCATCCCGC
GCTCAGCCTGCCGCTCGGCGTCGACAACAACGGGCTGCCGTTCGGGCTGCAGATCGTGGGCCCCCGCGGCGGCGATGCGC
TGGTGCTCGGCGTGGCCGCGGCGCTGGAGCAGGCGTTCGCCGGCGATGCCGAGCTGCGCCGTCCGCAGCCGGATCTGACC
AGGCTCGCCGCGTTGCCGCCGCTGTCGCAGACGCCCGGCTTCATGGCCTGGGATTGA

Upstream 100 bases:

>100_bases
ATGGGCATGCATGAACTGCGGTGGAACTGTGCGTTTTCGCCTCGTGCATATCATTTCTTATGTGCTAAATAGTTTTCCGA
TTTGGAAAGAGAAGTGCTCC

Downstream 100 bases:

>100_bases
AGCGTGGCATCATCAATACAGTCTTCTGGAGCTAGCAATGTCCGACGCAAACTCATGGCCGTTCTCGCTGGTGCGCCGCG
CCGGCGGTCTCGTGTTCCTG

Product: putative amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 498; Mature: 497

Protein sequence:

>498_residues
MTALCDQTANELVKLLKFKAISPVALLDSCLERIGAVNPAVNAVVTLDEPGARAAARRAESELLRGEDKGPLHGLPVLIK
DTQDTAGMRSTYGSPILAGNIPAIDQASVARLRAAGAIIFGKTNTPEWAAGGNTRNPVFGATGNPFDTLRSAAGSSGGSA
AALACGMAPLASGSDTGGSLRNPAGFCGIVGFRPSYGLVASEKRAHGWSCLSTDGPMARDVADTALMLSVMASDDVRDPL
AYTLPGEAVRGQPARWAVPPQVELRKLRLAFTEDFNFAPTEQLIRRAFRARVSRIAPLFAEAAEAAPDCSGADETFAVLR
ASLFLTQHGKNYRERPEMIGPNVRANVEEGLSYSLADFSRAAANQTRIYRAYQSFFAKHDVLISPTITLSPRPWSELYPA
EIDGAPTKSYFHWLALAYAVTLAGHPALSLPLGVDNNGLPFGLQIVGPRGGDALVLGVAAALEQAFAGDAELRRPQPDLT
RLAALPPLSQTPGFMAWD

Sequences:

>Translated_498_residues
MTALCDQTANELVKLLKFKAISPVALLDSCLERIGAVNPAVNAVVTLDEPGARAAARRAESELLRGEDKGPLHGLPVLIK
DTQDTAGMRSTYGSPILAGNIPAIDQASVARLRAAGAIIFGKTNTPEWAAGGNTRNPVFGATGNPFDTLRSAAGSSGGSA
AALACGMAPLASGSDTGGSLRNPAGFCGIVGFRPSYGLVASEKRAHGWSCLSTDGPMARDVADTALMLSVMASDDVRDPL
AYTLPGEAVRGQPARWAVPPQVELRKLRLAFTEDFNFAPTEQLIRRAFRARVSRIAPLFAEAAEAAPDCSGADETFAVLR
ASLFLTQHGKNYRERPEMIGPNVRANVEEGLSYSLADFSRAAANQTRIYRAYQSFFAKHDVLISPTITLSPRPWSELYPA
EIDGAPTKSYFHWLALAYAVTLAGHPALSLPLGVDNNGLPFGLQIVGPRGGDALVLGVAAALEQAFAGDAELRRPQPDLT
RLAALPPLSQTPGFMAWD
>Mature_497_residues
TALCDQTANELVKLLKFKAISPVALLDSCLERIGAVNPAVNAVVTLDEPGARAAARRAESELLRGEDKGPLHGLPVLIKD
TQDTAGMRSTYGSPILAGNIPAIDQASVARLRAAGAIIFGKTNTPEWAAGGNTRNPVFGATGNPFDTLRSAAGSSGGSAA
ALACGMAPLASGSDTGGSLRNPAGFCGIVGFRPSYGLVASEKRAHGWSCLSTDGPMARDVADTALMLSVMASDDVRDPLA
YTLPGEAVRGQPARWAVPPQVELRKLRLAFTEDFNFAPTEQLIRRAFRARVSRIAPLFAEAAEAAPDCSGADETFAVLRA
SLFLTQHGKNYRERPEMIGPNVRANVEEGLSYSLADFSRAAANQTRIYRAYQSFFAKHDVLISPTITLSPRPWSELYPAE
IDGAPTKSYFHWLALAYAVTLAGHPALSLPLGVDNNGLPFGLQIVGPRGGDALVLGVAAALEQAFAGDAELRRPQPDLTR
LAALPPLSQTPGFMAWD

Specific function: Unknown

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=504, Percent_Identity=26.3888888888889, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI195972892, Length=498, Percent_Identity=26.3052208835341, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI166795287, Length=281, Percent_Identity=30.6049822064057, Blast_Score=84, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17537465, Length=506, Percent_Identity=26.6798418972332, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17543272, Length=418, Percent_Identity=27.2727272727273, Blast_Score=118, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI17538252, Length=281, Percent_Identity=30.2491103202847, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17556264, Length=233, Percent_Identity=32.1888412017167, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI71990152, Length=238, Percent_Identity=31.9327731092437, Blast_Score=95, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17538254, Length=267, Percent_Identity=27.3408239700375, Blast_Score=75, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17556276, Length=198, Percent_Identity=32.8282828282828, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17556278, Length=198, Percent_Identity=32.8282828282828, Blast_Score=72, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=472, Percent_Identity=24.1525423728814, Blast_Score=93, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6319685, Length=394, Percent_Identity=27.1573604060914, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6320448, Length=450, Percent_Identity=24.4444444444444, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21356731, Length=493, Percent_Identity=26.5720081135903, Blast_Score=136, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24644968, Length=507, Percent_Identity=27.4161735700197, Blast_Score=128, Evalue=9e-30,
Organism=Drosophila melanogaster, GI24648435, Length=505, Percent_Identity=26.1386138613861, Blast_Score=127, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24648437, Length=505, Percent_Identity=26.1386138613861, Blast_Score=127, Evalue=3e-29,
Organism=Drosophila melanogaster, GI45550774, Length=505, Percent_Identity=26.1386138613861, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24652985, Length=521, Percent_Identity=26.4875239923225, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI19922090, Length=521, Percent_Identity=26.4875239923225, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24652981, Length=521, Percent_Identity=26.4875239923225, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24652983, Length=521, Percent_Identity=26.4875239923225, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI161078093, Length=504, Percent_Identity=27.7777777777778, Blast_Score=118, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24648113, Length=476, Percent_Identity=26.890756302521, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24648441, Length=433, Percent_Identity=26.3279445727483, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24648439, Length=433, Percent_Identity=26.3279445727483, Blast_Score=106, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.1.4 [H]

Molecular weight: Translated: 52630; Mature: 52499

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTALCDQTANELVKLLKFKAISPVALLDSCLERIGAVNPAVNAVVTLDEPGARAAARRAE
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHH
SELLRGEDKGPLHGLPVLIKDTQDTAGMRSTYGSPILAGNIPAIDQASVARLRAAGAIIF
HHHHCCCCCCCCCCCCEEEECCCCCCCHHHHCCCCEEECCCCCCCHHHHHHHHHCCEEEE
GKTNTPEWAAGGNTRNPVFGATGNPFDTLRSAAGSSGGSAAALACGMAPLASGSDTGGSL
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCCCCC
RNPAGFCGIVGFRPSYGLVASEKRAHGWSCLSTDGPMARDVADTALMLSVMASDDVRDPL
CCCCCEEEEEECCCCCCCEECCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCC
AYTLPGEAVRGQPARWAVPPQVELRKLRLAFTEDFNFAPTEQLIRRAFRARVSRIAPLFA
EECCCCHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
EAAEAAPDCSGADETFAVLRASLFLTQHGKNYRERPEMIGPNVRANVEEGLSYSLADFSR
HHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHCHHHCCCCCCCCHHHCCCHHHHHHHH
AAANQTRIYRAYQSFFAKHDVLISPTITLSPRPWSELYPAEIDGAPTKSYFHWLALAYAV
HHHHHHHHHHHHHHHHHCCCEEECCEEEECCCCCHHCCCCCCCCCCCHHHHHHHHHHHHH
TLAGHPALSLPLGVDNNGLPFGLQIVGPRGGDALVLGVAAALEQAFAGDAELRRPQPDLT
HHCCCCCEEEECCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHH
RLAALPPLSQTPGFMAWD
HHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TALCDQTANELVKLLKFKAISPVALLDSCLERIGAVNPAVNAVVTLDEPGARAAARRAE
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHH
SELLRGEDKGPLHGLPVLIKDTQDTAGMRSTYGSPILAGNIPAIDQASVARLRAAGAIIF
HHHHCCCCCCCCCCCCEEEECCCCCCCHHHHCCCCEEECCCCCCCHHHHHHHHHCCEEEE
GKTNTPEWAAGGNTRNPVFGATGNPFDTLRSAAGSSGGSAAALACGMAPLASGSDTGGSL
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCCCCC
RNPAGFCGIVGFRPSYGLVASEKRAHGWSCLSTDGPMARDVADTALMLSVMASDDVRDPL
CCCCCEEEEEECCCCCCCEECCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCC
AYTLPGEAVRGQPARWAVPPQVELRKLRLAFTEDFNFAPTEQLIRRAFRARVSRIAPLFA
EECCCCHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
EAAEAAPDCSGADETFAVLRASLFLTQHGKNYRERPEMIGPNVRANVEEGLSYSLADFSR
HHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHCHHHCCCCCCCCHHHCCCHHHHHHHH
AAANQTRIYRAYQSFFAKHDVLISPTITLSPRPWSELYPAEIDGAPTKSYFHWLALAYAV
HHHHHHHHHHHHHHHHHCCCEEECCEEEECCCCCHHCCCCCCCCCCCHHHHHHHHHHHHH
TLAGHPALSLPLGVDNNGLPFGLQIVGPRGGDALVLGVAAALEQAFAGDAELRRPQPDLT
HHCCCCCEEEECCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHH
RLAALPPLSQTPGFMAWD
HHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]