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The map label for this gene is nadB [H]

Identifier: 146341439

GI number: 146341439

Start: 4729425

End: 4730978

Strand: Reverse

Name: nadB [H]

Synonym: BRADO4530

Alternate gene names: 146341439

Gene position: 4730978-4729425 (Counterclockwise)

Preceding gene: 146341440

Following gene: 146341438

Centisome position: 63.45

GC content: 70.72

Gene sequence:

>1554_bases
ATGAGCACCGATCTCTCCAGTCTCATCGGCCGTCCCGTGATCATCGGCGGCGGCGCCGCCGGGCTGATGACGGCGCTGCA
ACTGGCACCCGAGCCCGTCGTACTCGTGTCGAAGTCGCCGCTCGGCGCGGAGGCGTCGAGCATGTGGGCGCAGGGTGGTC
TCGCGGCTGCGGTCGGTCGCGACGATGAGCCGGCGCTGCATTTCGCCGATACGATTGCCGCCGGCGCAGGCCTGTGTGAC
GAGGCCGCGGCGCGTCGCATCATCGATGCTGCGCCTGCGGCGGTCGAGCGCCTCGCGGAGCTCGGCGTCGCGTTCGACCG
CGATGCCGACGGGCGCTGGCGCCTCGGCCTCGAGGCGGCACACAGCCGCAAGCGCATCGTGCACGCCACCGGTGACGGCA
CCGGGCGCGAGATCATGCGCGCGCTGATCGCGGCCGTCAGGCGATGTCCGTCGGTGACCTTGCTCGAAGGTGTCGAGGCG
CGCCGGCTGATCGTCGTGGACAATGCGATCCAGGGGGTGGTCGCGGTGAGCGGCGAGGAGCCGGTCGTGCTCGCCACCTC
GCGCGTCGTGATCGCGACCGGCGGCATCGGCGGCCTGTTCCTCGACAGCACCAATCCGTCCGGCTGCTTCGGCCAGGGGT
TGGCGCTGGCGGCGCGGGCAGGGGCGGTGCTGTCCGATCTCGAATTCGTGCAGTTTCATCCCACCGCCTTCGACGGTCCG
TCGCGGCCGCTGCCGCTGCTGACCGAGGCGATCCGCGGCGATGGCGCGGTGTTGATCGACGAGACCGGCACGCGCTTCAT
GGCGGACGAGCCCGGCGCCGAGCTGGCGCCACGCGACGTGGTGGCGCGGGCGGTGTGGCGTCAGCGCGCGCAGGGCCATC
GCGTCTTTCTCGATGCGCGCGAGGCGCTGGGGGGCACCTTCGCCGCGCGCTATCCCGTGATCAGCGCGTTCTGCCGCATG
GCCGGAATTGATCCGGCCGTCGATCCCATACCGGTTCGACCCGCCGTGCACTACCATATGGGCGGTATCTCCGTTGATGC
GATGGGCCGCAGTTCGGTCGACGGGTTGTGGGCTTGCGGCGAGGTCAGCCGCACCGGACTGCACGGCGCCAATCGCCTCG
CCAGCAACTCGCTGATGGAAGCAATCGTCTGCGCGCAATGGGTGGCCGAGAGCACGGCCGGGACGCCGCGGCGCGCTGCC
AAGGTCGGAGAGCCCGCGGCAAGCCCCGCTGCATCTGATCCGTCCTCGGTGCGTCCGCTGCTGTCCGGCGCACTCGGCGT
GCTGCGCGACCGCGATCGGATGGTGTCCGCCATCCGGAGCCTGCTCCCGCTGGCGATAGGAGCTGGAGCGACGGCCGATC
CAGCCCTGGTCGGGCTGATGATGTCGGTGGCTGCCCATGCGCGCGAGGAGAGCCGCGGCGGGCATTGCCGCACCGACTGT
CCGGACACGCTGCCCGCCGCGCAGCCGTCCCGTTTTACGTCGTCCTCAGCGATCGCCGCCGCGTGGGAGATTGCGGAGAC
AGGGCATCCTTCATTCAGGAGCATCGTATCATGA

Upstream 100 bases:

>100_bases
AGAACATCCGTCATGCGCTCGAGACCAACACGCATGAGGTGACGATCGATCCCGCGATGGCCGAGCGCGCGCGGCGTTCA
GTGGAAAGGATGCTGGCGCT

Downstream 100 bases:

>100_bases
GCCTCAATCCACTCTTTCCCTTGCTCTACGAGCCACTGCTCCGTGCGGCGCTGCTGGAGGATCTCGGCCGCGCCGGCGAC
ATCACCACCGATGCGATCGT

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 517; Mature: 516

Protein sequence:

>517_residues
MSTDLSSLIGRPVIIGGGAAGLMTALQLAPEPVVLVSKSPLGAEASSMWAQGGLAAAVGRDDEPALHFADTIAAGAGLCD
EAAARRIIDAAPAAVERLAELGVAFDRDADGRWRLGLEAAHSRKRIVHATGDGTGREIMRALIAAVRRCPSVTLLEGVEA
RRLIVVDNAIQGVVAVSGEEPVVLATSRVVIATGGIGGLFLDSTNPSGCFGQGLALAARAGAVLSDLEFVQFHPTAFDGP
SRPLPLLTEAIRGDGAVLIDETGTRFMADEPGAELAPRDVVARAVWRQRAQGHRVFLDAREALGGTFAARYPVISAFCRM
AGIDPAVDPIPVRPAVHYHMGGISVDAMGRSSVDGLWACGEVSRTGLHGANRLASNSLMEAIVCAQWVAESTAGTPRRAA
KVGEPAASPAASDPSSVRPLLSGALGVLRDRDRMVSAIRSLLPLAIGAGATADPALVGLMMSVAAHAREESRGGHCRTDC
PDTLPAAQPSRFTSSSAIAAAWEIAETGHPSFRSIVS

Sequences:

>Translated_517_residues
MSTDLSSLIGRPVIIGGGAAGLMTALQLAPEPVVLVSKSPLGAEASSMWAQGGLAAAVGRDDEPALHFADTIAAGAGLCD
EAAARRIIDAAPAAVERLAELGVAFDRDADGRWRLGLEAAHSRKRIVHATGDGTGREIMRALIAAVRRCPSVTLLEGVEA
RRLIVVDNAIQGVVAVSGEEPVVLATSRVVIATGGIGGLFLDSTNPSGCFGQGLALAARAGAVLSDLEFVQFHPTAFDGP
SRPLPLLTEAIRGDGAVLIDETGTRFMADEPGAELAPRDVVARAVWRQRAQGHRVFLDAREALGGTFAARYPVISAFCRM
AGIDPAVDPIPVRPAVHYHMGGISVDAMGRSSVDGLWACGEVSRTGLHGANRLASNSLMEAIVCAQWVAESTAGTPRRAA
KVGEPAASPAASDPSSVRPLLSGALGVLRDRDRMVSAIRSLLPLAIGAGATADPALVGLMMSVAAHAREESRGGHCRTDC
PDTLPAAQPSRFTSSSAIAAAWEIAETGHPSFRSIVS
>Mature_516_residues
STDLSSLIGRPVIIGGGAAGLMTALQLAPEPVVLVSKSPLGAEASSMWAQGGLAAAVGRDDEPALHFADTIAAGAGLCDE
AAARRIIDAAPAAVERLAELGVAFDRDADGRWRLGLEAAHSRKRIVHATGDGTGREIMRALIAAVRRCPSVTLLEGVEAR
RLIVVDNAIQGVVAVSGEEPVVLATSRVVIATGGIGGLFLDSTNPSGCFGQGLALAARAGAVLSDLEFVQFHPTAFDGPS
RPLPLLTEAIRGDGAVLIDETGTRFMADEPGAELAPRDVVARAVWRQRAQGHRVFLDAREALGGTFAARYPVISAFCRMA
GIDPAVDPIPVRPAVHYHMGGISVDAMGRSSVDGLWACGEVSRTGLHGANRLASNSLMEAIVCAQWVAESTAGTPRRAAK
VGEPAASPAASDPSSVRPLLSGALGVLRDRDRMVSAIRSLLPLAIGAGATADPALVGLMMSVAAHAREESRGGHCRTDCP
DTLPAAQPSRFTSSSAIAAAWEIAETGHPSFRSIVS

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=526, Percent_Identity=30.4182509505703, Blast_Score=176, Evalue=4e-44,
Organism=Escherichia coli, GI1788928, Length=520, Percent_Identity=37.3076923076923, Blast_Score=266, Evalue=3e-72,
Organism=Escherichia coli, GI1790597, Length=467, Percent_Identity=30.8351177730193, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI1786942, Length=506, Percent_Identity=30.0395256916996, Blast_Score=148, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17550100, Length=513, Percent_Identity=31.5789473684211, Blast_Score=177, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI17505833, Length=500, Percent_Identity=29.8, Blast_Score=162, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI71986328, Length=437, Percent_Identity=27.9176201372998, Blast_Score=91, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6322416, Length=501, Percent_Identity=28.9421157684631, Blast_Score=166, Evalue=7e-42,
Organism=Saccharomyces cerevisiae, GI6322701, Length=503, Percent_Identity=27.6341948310139, Blast_Score=165, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6320788, Length=476, Percent_Identity=28.1512605042017, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI17137288, Length=506, Percent_Identity=30.6324110671937, Blast_Score=172, Evalue=4e-43,
Organism=Drosophila melanogaster, GI24655642, Length=506, Percent_Identity=30.6324110671937, Blast_Score=172, Evalue=4e-43,
Organism=Drosophila melanogaster, GI24655647, Length=506, Percent_Identity=30.6324110671937, Blast_Score=172, Evalue=4e-43,
Organism=Drosophila melanogaster, GI24663005, Length=366, Percent_Identity=32.2404371584699, Blast_Score=149, Evalue=4e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 53335; Mature: 53204

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTDLSSLIGRPVIIGGGAAGLMTALQLAPEPVVLVSKSPLGAEASSMWAQGGLAAAVGR
CCCCHHHHHCCCEEEECCHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHCCCEEEECCC
DDEPALHFADTIAAGAGLCDEAAARRIIDAAPAAVERLAELGVAFDRDADGRWRLGLEAA
CCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCEEECHHHH
HSRKRIVHATGDGTGREIMRALIAAVRRCPSVTLLEGVEARRLIVVDNAIQGVVAVSGEE
CCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHCCCCCCEEEEEECCCCCEEEECCCC
PVVLATSRVVIATGGIGGLFLDSTNPSGCFGQGLALAARAGAVLSDLEFVQFHPTAFDGP
CEEEEECEEEEEECCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCHHEEECCCCCCCC
SRPLPLLTEAIRGDGAVLIDETGTRFMADEPGAELAPRDVVARAVWRQRAQGHRVFLDAR
CCCHHHHHHHHCCCCEEEEECCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECH
EALGGTFAARYPVISAFCRMAGIDPAVDPIPVRPAVHYHMGGISVDAMGRSSVDGLWACG
HHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCEEEECCCCCCCCCEEECC
EVSRTGLHGANRLASNSLMEAIVCAQWVAESTAGTPRRAAKVGEPAASPAASDPSSVRPL
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCHHHHHHH
LSGALGVLRDRDRMVSAIRSLLPLAIGAGATADPALVGLMMSVAAHAREESRGGHCRTDC
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
PDTLPAAQPSRFTSSSAIAAAWEIAETGHPSFRSIVS
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHCC
>Mature Secondary Structure 
STDLSSLIGRPVIIGGGAAGLMTALQLAPEPVVLVSKSPLGAEASSMWAQGGLAAAVGR
CCCHHHHHCCCEEEECCHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHCCCEEEECCC
DDEPALHFADTIAAGAGLCDEAAARRIIDAAPAAVERLAELGVAFDRDADGRWRLGLEAA
CCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCEEECHHHH
HSRKRIVHATGDGTGREIMRALIAAVRRCPSVTLLEGVEARRLIVVDNAIQGVVAVSGEE
CCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHCCCCCCEEEEEECCCCCEEEECCCC
PVVLATSRVVIATGGIGGLFLDSTNPSGCFGQGLALAARAGAVLSDLEFVQFHPTAFDGP
CEEEEECEEEEEECCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCHHEEECCCCCCCC
SRPLPLLTEAIRGDGAVLIDETGTRFMADEPGAELAPRDVVARAVWRQRAQGHRVFLDAR
CCCHHHHHHHHCCCCEEEEECCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECH
EALGGTFAARYPVISAFCRMAGIDPAVDPIPVRPAVHYHMGGISVDAMGRSSVDGLWACG
HHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCEEEECCCCCCCCCEEECC
EVSRTGLHGANRLASNSLMEAIVCAQWVAESTAGTPRRAAKVGEPAASPAASDPSSVRPL
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCHHHHHHH
LSGALGVLRDRDRMVSAIRSLLPLAIGAGATADPALVGLMMSVAAHAREESRGGHCRTDC
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
PDTLPAAQPSRFTSSSAIAAAWEIAETGHPSFRSIVS
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]