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The map label for this gene is pdxJ

Identifier: 146341380

GI number: 146341380

Start: 4666199

End: 4666969

Strand: Reverse

Name: pdxJ

Synonym: BRADO4467

Alternate gene names: 146341380

Gene position: 4666969-4666199 (Counterclockwise)

Preceding gene: 146341381

Following gene: 146341379

Centisome position: 62.59

GC content: 70.43

Gene sequence:

>771_bases
ATGTCCCATCCCCCGAAGCTGCGCCTCGGCGTCAATGTCGACCACATCGCTACGTTGCGGAATGCGCGCGGCGGCCGTGC
GCCGGATCCGGTACGCGCTGCGCTGCTCGCCATCGAGGCCGGCGCCGACGGCATCACCGCGCATCTGCGCGAGGACCGGC
GGCACATCCGCGACGACGACATGGCCCGCCTCAAGGCGCAGATCTCCAGGCCGCTGAACTTCGAGATGGCGGCCACCGAG
GAGATGGTGCGGATCGCGCTGGCGACGAAGCCGCATGCCTGCTGCCTGGTGCCCGAGCGGCGCGAGGAACTGACCACCGA
GGGCGGGCTCGACGTCGTCGGCCAGCACAATGCGCTGGCGCCGGCGATCGCCCGGCTCAGCGAGGCCGGCATCCGGGTCT
CGCTGTTCATCGCCGCCGACCCCGCCCAGATCGAGATGGCGGCGCGGCTGCGCGCGCCAGTCATCGAGATCCATACCGGC
GGCTGGTGCGACGCGATCACCGATGGCCACCATGACAAGGCGGACGCCGAATGGCAGCGCATCGTGGCCGGTGCCCGCCA
AGCCTATGCCGCAGGGCTCGAGGTCCATGCCGGCCATGGCCTGGATTACGCCACCGCCGAGCAGATCGCGGCCTTGCCTG
AAATCCGCGAGCTGAACATCGGCTACTTCATAATGGGCGAGGCGCTGTTCGTGGGCCTGGCCGAGAGCGTGCGGACGATG
CGCGCGGCGATGGATCGGGGCCGCGACCGGGCGACAGGGGCCACGGCATGA

Upstream 100 bases:

>100_bases
GACCGTGTGAATGGCTAATCTGCGGGTTTGGCCGCAGCGTTTCCTCCGGCGATGAGCTAAACCAGCAATTCCGTTTTGAG
AGAACACCGCGAGCCTGCCG

Downstream 100 bases:

>100_bases
TCATCGGCATCGGCTCGGACCTGATCGACATCCGCAGGGTCGGCGAGGTGATCGAGCGCCACGGCGAGCGCTTCCTGAAC
CGGATCTTCACCGAGGCCGA

Product: pyridoxine 5'-phosphate synthase

Products: NA

Alternate protein names: PNP synthase

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MSHPPKLRLGVNVDHIATLRNARGGRAPDPVRAALLAIEAGADGITAHLREDRRHIRDDDMARLKAQISRPLNFEMAATE
EMVRIALATKPHACCLVPERREELTTEGGLDVVGQHNALAPAIARLSEAGIRVSLFIAADPAQIEMAARLRAPVIEIHTG
GWCDAITDGHHDKADAEWQRIVAGARQAYAAGLEVHAGHGLDYATAEQIAALPEIRELNIGYFIMGEALFVGLAESVRTM
RAAMDRGRDRATGATA

Sequences:

>Translated_256_residues
MSHPPKLRLGVNVDHIATLRNARGGRAPDPVRAALLAIEAGADGITAHLREDRRHIRDDDMARLKAQISRPLNFEMAATE
EMVRIALATKPHACCLVPERREELTTEGGLDVVGQHNALAPAIARLSEAGIRVSLFIAADPAQIEMAARLRAPVIEIHTG
GWCDAITDGHHDKADAEWQRIVAGARQAYAAGLEVHAGHGLDYATAEQIAALPEIRELNIGYFIMGEALFVGLAESVRTM
RAAMDRGRDRATGATA
>Mature_255_residues
SHPPKLRLGVNVDHIATLRNARGGRAPDPVRAALLAIEAGADGITAHLREDRRHIRDDDMARLKAQISRPLNFEMAATEE
MVRIALATKPHACCLVPERREELTTEGGLDVVGQHNALAPAIARLSEAGIRVSLFIAADPAQIEMAARLRAPVIEIHTGG
WCDAITDGHHDKADAEWQRIVAGARQAYAAGLEVHAGHGLDYATAEQIAALPEIRELNIGYFIMGEALFVGLAESVRTMR
AAMDRGRDRATGATA

Specific function: Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate

COG id: COG0854

COG function: function code H; Pyridoxal phosphate biosynthesis protein

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP synthase family

Homologues:

Organism=Escherichia coli, GI1788917, Length=243, Percent_Identity=55.9670781893004, Blast_Score=259, Evalue=1e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXJ_BRASO (A4YWD1)

Other databases:

- EMBL:   CU234118
- RefSeq:   YP_001206428.1
- ProteinModelPortal:   A4YWD1
- SMR:   A4YWD1
- STRING:   A4YWD1
- GeneID:   5115078
- GenomeReviews:   CU234118_GR
- KEGG:   bra:BRADO4467
- eggNOG:   COG0854
- HOGENOM:   HBG299314
- OMA:   AGHGLNY
- ProtClustDB:   PRK05265
- BioCyc:   BSP376:BRADO4467-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00279
- InterPro:   IPR013785
- InterPro:   IPR004569
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00559

Pfam domain/function: PF03740 PdxJ; SSF63892 PyrdxlP_synth_PdxJ

EC number: =2.6.99.2

Molecular weight: Translated: 27615; Mature: 27484

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: ACT_SITE 48-48 ACT_SITE 75-75 ACT_SITE 199-199 BINDING 12-12 BINDING 23-23 BINDING 50-50 BINDING 55-55 BINDING 105-105 BINDING 200-200

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHPPKLRLGVNVDHIATLRNARGGRAPDPVRAALLAIEAGADGITAHLREDRRHIRDDD
CCCCCCEEECCCHHHHHHHHHCCCCCCCCHHHHHHHHEECCCCCHHHHHHHHHHHCCCCH
MARLKAQISRPLNFEMAATEEMVRIALATKPHACCLVPERREELTTEGGLDVVGQHNALA
HHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEECCHHHHHHCCCCCCEEECCCCHHH
PAIARLSEAGIRVSLFIAADPAQIEMAARLRAPVIEIHTGGWCDAITDGHHDKADAEWQR
HHHHHHHHCCCEEEEEEECCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCHHHHHH
IVAGARQAYAAGLEVHAGHGLDYATAEQIAALPEIRELNIGYFIMGEALFVGLAESVRTM
HHHHHHHHHHCCCEEECCCCCCHHHHHHHHHCCCHHHCCCCEEEHHHHHHHHHHHHHHHH
RAAMDRGRDRATGATA
HHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SHPPKLRLGVNVDHIATLRNARGGRAPDPVRAALLAIEAGADGITAHLREDRRHIRDDD
CCCCCEEECCCHHHHHHHHHCCCCCCCCHHHHHHHHEECCCCCHHHHHHHHHHHCCCCH
MARLKAQISRPLNFEMAATEEMVRIALATKPHACCLVPERREELTTEGGLDVVGQHNALA
HHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEECCHHHHHHCCCCCCEEECCCCHHH
PAIARLSEAGIRVSLFIAADPAQIEMAARLRAPVIEIHTGGWCDAITDGHHDKADAEWQR
HHHHHHHHCCCEEEEEEECCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCHHHHHH
IVAGARQAYAAGLEVHAGHGLDYATAEQIAALPEIRELNIGYFIMGEALFVGLAESVRTM
HHHHHHHHHHCCCEEECCCCCCHHHHHHHHHCCCHHHCCCCEEEHHHHHHHHHHHHHHHH
RAAMDRGRDRATGATA
HHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA