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The map label for this gene is lepB [H]

Identifier: 146341378

GI number: 146341378

Start: 4664735

End: 4665496

Strand: Reverse

Name: lepB [H]

Synonym: BRADO4464

Alternate gene names: 146341378

Gene position: 4665496-4664735 (Counterclockwise)

Preceding gene: 146341379

Following gene: 146341377

Centisome position: 62.57

GC content: 60.24

Gene sequence:

>762_bases
ATGAGCGTGACCTCCGGAACCAAGACTGAGAGCGGCATCGGTGAAACCGTTCGTGTGGTCGTTCATGCCCTCCTGATCGC
ACTCGTGATCCGCACGTTCCTGTTCCAGCCCTTCAACATCCCCTCGGGATCAATGAAGGCGACCTTGCTGGTCGGCGATT
ATCTGTTCGTGTCGAAATATTCGTACGGCTATAGCCATTTCTCGATTCCGCAGTCGCCCAACATCTTCTCGGGCCGCATC
TTCGGCTCGGAGCCTCGGCGCGGCGACATCGTCGTGTTTCGCCTGCCGAAGGACACCGAAATCGACTACATCAAGCGCGT
GATCGGACTGCCGGGCGACCGCATCCAGATGCGTGACGGCCTGCTGTACATCAACGACGCGCCGGTCCAGCGCGAGCGGC
TGACCGACTTCGTCGGCGAGGATCCCTGCGGCACCGCCGATTCGATCGCGCGGGTGAAGCGCTGGAAGGAGACGCTGCCG
AACGGCGTCAGCTACGAAACCCTGGATTGCTTCGACCACGGCCCTTACGATAGTACGGACGTGTATACGGTGCCGCCCGG
CAACTTCTTCATGATGGGCGACAACCGCGACAACTCGACTGACAGCCGTGTTCAGACGGCGGTCGGCTATGTCCCGTTCG
AGAACATCATCGGCCGCGCCCAGATGATCTTCTTCTCCATTCGGGAGAACGAGCATGCCTGGGCGTTCTGGCGCTGGCCG
ATGTCCATCCGTTGGGAACGTCTGTTCAAGATCGTACGATGA

Upstream 100 bases:

>100_bases
TCCCTTAAGCTGCGGACCCATACGTTGTTCGCGGTCGGCGGGGTGTTATGCAACGGCTGGCGAATCATCGGACCATTCGC
CCAGGGACAGTGAAAGAGCG

Downstream 100 bases:

>100_bases
TCGACGACTCACCCGAGATCCAGACGACGCAGGCTGCTGCAACGAGCCCGCAAGCCGGCGCGGGCGCGCTCGAGGCTGCG
CCCAAGACGTCGACGCGACC

Product: signal peptidase I

Products: NA

Alternate protein names: SPase I; Leader peptidase I [H]

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MSVTSGTKTESGIGETVRVVVHALLIALVIRTFLFQPFNIPSGSMKATLLVGDYLFVSKYSYGYSHFSIPQSPNIFSGRI
FGSEPRRGDIVVFRLPKDTEIDYIKRVIGLPGDRIQMRDGLLYINDAPVQRERLTDFVGEDPCGTADSIARVKRWKETLP
NGVSYETLDCFDHGPYDSTDVYTVPPGNFFMMGDNRDNSTDSRVQTAVGYVPFENIIGRAQMIFFSIRENEHAWAFWRWP
MSIRWERLFKIVR

Sequences:

>Translated_253_residues
MSVTSGTKTESGIGETVRVVVHALLIALVIRTFLFQPFNIPSGSMKATLLVGDYLFVSKYSYGYSHFSIPQSPNIFSGRI
FGSEPRRGDIVVFRLPKDTEIDYIKRVIGLPGDRIQMRDGLLYINDAPVQRERLTDFVGEDPCGTADSIARVKRWKETLP
NGVSYETLDCFDHGPYDSTDVYTVPPGNFFMMGDNRDNSTDSRVQTAVGYVPFENIIGRAQMIFFSIRENEHAWAFWRWP
MSIRWERLFKIVR
>Mature_252_residues
SVTSGTKTESGIGETVRVVVHALLIALVIRTFLFQPFNIPSGSMKATLLVGDYLFVSKYSYGYSHFSIPQSPNIFSGRIF
GSEPRRGDIVVFRLPKDTEIDYIKRVIGLPGDRIQMRDGLLYINDAPVQRERLTDFVGEDPCGTADSIARVKRWKETLPN
GVSYETLDCFDHGPYDSTDVYTVPPGNFFMMGDNRDNSTDSRVQTAVGYVPFENIIGRAQMIFFSIRENEHAWAFWRWPM
SIRWERLFKIVR

Specific function: Unknown

COG id: COG0681

COG function: function code U; Signal peptidase I

Gene ontology:

Cell location: Cell membrane; Single-pass type II membrane protein (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S26 family [H]

Homologues:

Organism=Escherichia coli, GI1788921, Length=273, Percent_Identity=35.5311355311355, Blast_Score=143, Evalue=9e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000223
- InterPro:   IPR019758
- InterPro:   IPR019757
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056 [H]

Pfam domain/function: PF00717 Peptidase_S24 [H]

EC number: =3.4.21.89 [H]

Molecular weight: Translated: 28885; Mature: 28754

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: PS00760 SPASE_I_2 ; PS00761 SPASE_I_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVTSGTKTESGIGETVRVVVHALLIALVIRTFLFQPFNIPSGSMKATLLVGDYLFVSKY
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECEEEEEEC
SYGYSHFSIPQSPNIFSGRIFGSEPRRGDIVVFRLPKDTEIDYIKRVIGLPGDRIQMRDG
CCCCCEECCCCCCCEEECEEECCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCEEEEECC
LLYINDAPVQRERLTDFVGEDPCGTADSIARVKRWKETLPNGVSYETLDCFDHGPYDSTD
EEEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCC
VYTVPPGNFFMMGDNRDNSTDSRVQTAVGYVPFENIIGRAQMIFFSIRENEHAWAFWRWP
EEEECCCCEEEEECCCCCCCHHHHHHHHCCCCHHHHHCCEEEEEEEEECCCCEEEEEECC
MSIRWERLFKIVR
CCCCHHHHHHHCC
>Mature Secondary Structure 
SVTSGTKTESGIGETVRVVVHALLIALVIRTFLFQPFNIPSGSMKATLLVGDYLFVSKY
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECEEEEEEC
SYGYSHFSIPQSPNIFSGRIFGSEPRRGDIVVFRLPKDTEIDYIKRVIGLPGDRIQMRDG
CCCCCEECCCCCCCEEECEEECCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCEEEEECC
LLYINDAPVQRERLTDFVGEDPCGTADSIARVKRWKETLPNGVSYETLDCFDHGPYDSTD
EEEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCC
VYTVPPGNFFMMGDNRDNSTDSRVQTAVGYVPFENIIGRAQMIFFSIRENEHAWAFWRWP
EEEECCCCEEEEECCCCCCCHHHHHHHHCCCCHHHHHCCEEEEEEEEECCCCEEEEEECC
MSIRWERLFKIVR
CCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]