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The map label for this gene is mltB [H]

Identifier: 146340537

GI number: 146340537

Start: 3773829

End: 3775049

Strand: Reverse

Name: mltB [H]

Synonym: BRADO3580

Alternate gene names: 146340537

Gene position: 3775049-3773829 (Counterclockwise)

Preceding gene: 146340538

Following gene: 146340535

Centisome position: 50.63

GC content: 69.53

Gene sequence:

>1221_bases
ATGACCCAGCCCGATTCCCCGACCCGCCGCACCCTGCTCCGCTCCGGCCTCGGTGCCGCCGCGCTGCTCGTGCCTGCCGT
GCCGGCACTGGCCGCACCGGCCGAATTCGACGCCTGGCGCGATGGCTTCCGCGCCCGCGCGCTCGCCAAGGGCATCTCGC
AGGCGACCTGGACGCGCGTCATGGCGCGGCTCGAGCCCGACATGACTGTCTTCAGGGAGTTGCGCAATCAGCCGGAGTTC
AAGGAGCAGCTCTGGCAGTACGTCAATCGCCGCGTCTCCGACTGGCGCGTCATCAACGGCAAGGTCGCGCTGAAGCAGAA
CGAGGCGCTGTTCGCCCGAATCGAGAAGGATTTCGGCGTCGAGCGCGGCACGCTGCTGGCGCTATGGGGCGTCGAGTCGG
CCTATGGCGATCCGCTGGTGCAGCAGAACCACATGCGCCCGGTGTTTCCGGCGCTCGCCGCGCTCGCCTGGAACGAGCCG
CGCCGCAAGGCCTATTGGGAGACCGAGCTCACCAACGCGCTCAAAATTGTCGACCGCGGCTGGAGCACGCCGGAGGAGAT
GCGCGGCTCCTGGGCCGGCGCGATGGGGCATACGCAATGGATGCCGGAGGTCTGGCTCAATGTCGGCTTCGACTATGACG
GCGACGGCAAGATCTCGCCTTTCGGCAAGCCGGACGACGCGCTCGGCTCGACCGCGCGCTATCTCGTCAACCGCGGCAAG
TATCACCGCGGCGAGCATTGGGGCTACGAGGTGCGCGCGCCCGGCAACATGTCGGGCAGCAGGACCTACGCGGCCTGGCA
GGCCGCCGGCGTCACCCGCGCCGACGGCGAGCCGTTTCCGCAACCGAACGCGTCGGCGCAACTCTGGATCCCGGTCGAGG
GCGGCCCGGCCTTCCTGCTGGGGCCGAACTTCTACTCGGTGAAGTCCTACAATCCGGCGATGAGCTACGCGCTCGCGATC
TGCCATCTCGGCGACCGCATCCTGGGCGCCCCGCCCTTCCTGCAGCCCTTCCCCGGCTCCGAGCGCGCGCTCACGCTCGC
CGAAGTGCAGGAGATGCAGAAGCGCCTGACCGCGGCCGGCTTCGCGACCGGCGGCACCGACGGCCGCGTCGGCAACGACA
CGATGAAGGCGATCCGCGACTTCCAGGCCAAGGCCGGCCTCACGCCCGCCGACGGCTATGGCGGCCTGAAGGTGCTGACG
AAGCTGCGACAGGGCGGTTGA

Upstream 100 bases:

>100_bases
GATTCGACGCTGGCTGGCGCGCATCGCCGCCGAGTTCCATCCGGATTGAGCGCTGCGGGGGGTCTGCCGTCCGCAGGCCG
CAGCGGCTAGACTGCCGGAC

Downstream 100 bases:

>100_bases
CAGTTCAAGGTTCGTAGGGTGGGCAAAGGCGCTGCTGTGCTCTCGCCGCCTACGAGATCGTCGTGGCGCCGTGCCCACCC
TCCGCAATCCTGGAAAAGCT

Product: putative membrane-bound lytic transglycosylase protein signal peptide

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MTQPDSPTRRTLLRSGLGAAALLVPAVPALAAPAEFDAWRDGFRARALAKGISQATWTRVMARLEPDMTVFRELRNQPEF
KEQLWQYVNRRVSDWRVINGKVALKQNEALFARIEKDFGVERGTLLALWGVESAYGDPLVQQNHMRPVFPALAALAWNEP
RRKAYWETELTNALKIVDRGWSTPEEMRGSWAGAMGHTQWMPEVWLNVGFDYDGDGKISPFGKPDDALGSTARYLVNRGK
YHRGEHWGYEVRAPGNMSGSRTYAAWQAAGVTRADGEPFPQPNASAQLWIPVEGGPAFLLGPNFYSVKSYNPAMSYALAI
CHLGDRILGAPPFLQPFPGSERALTLAEVQEMQKRLTAAGFATGGTDGRVGNDTMKAIRDFQAKAGLTPADGYGGLKVLT
KLRQGG

Sequences:

>Translated_406_residues
MTQPDSPTRRTLLRSGLGAAALLVPAVPALAAPAEFDAWRDGFRARALAKGISQATWTRVMARLEPDMTVFRELRNQPEF
KEQLWQYVNRRVSDWRVINGKVALKQNEALFARIEKDFGVERGTLLALWGVESAYGDPLVQQNHMRPVFPALAALAWNEP
RRKAYWETELTNALKIVDRGWSTPEEMRGSWAGAMGHTQWMPEVWLNVGFDYDGDGKISPFGKPDDALGSTARYLVNRGK
YHRGEHWGYEVRAPGNMSGSRTYAAWQAAGVTRADGEPFPQPNASAQLWIPVEGGPAFLLGPNFYSVKSYNPAMSYALAI
CHLGDRILGAPPFLQPFPGSERALTLAEVQEMQKRLTAAGFATGGTDGRVGNDTMKAIRDFQAKAGLTPADGYGGLKVLT
KLRQGG
>Mature_405_residues
TQPDSPTRRTLLRSGLGAAALLVPAVPALAAPAEFDAWRDGFRARALAKGISQATWTRVMARLEPDMTVFRELRNQPEFK
EQLWQYVNRRVSDWRVINGKVALKQNEALFARIEKDFGVERGTLLALWGVESAYGDPLVQQNHMRPVFPALAALAWNEPR
RKAYWETELTNALKIVDRGWSTPEEMRGSWAGAMGHTQWMPEVWLNVGFDYDGDGKISPFGKPDDALGSTARYLVNRGKY
HRGEHWGYEVRAPGNMSGSRTYAAWQAAGVTRADGEPFPQPNASAQLWIPVEGGPAFLLGPNFYSVKSYNPAMSYALAIC
HLGDRILGAPPFLQPFPGSERALTLAEVQEMQKRLTAAGFATGGTDGRVGNDTMKAIRDFQAKAGLTPADGYGGLKVLTK
LRQGG

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=293, Percent_Identity=29.0102389078498, Blast_Score=102, Evalue=6e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 44764; Mature: 44633

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQPDSPTRRTLLRSGLGAAALLVPAVPALAAPAEFDAWRDGFRARALAKGISQATWTRV
CCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MARLEPDMTVFRELRNQPEFKEQLWQYVNRRVSDWRVINGKVALKQNEALFARIEKDFGV
HHHCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEECCEEEEECCCHHHHHHHHHHCC
ERGTLLALWGVESAYGDPLVQQNHMRPVFPALAALAWNEPRRKAYWETELTNALKIVDRG
CCCCEEEEECCCHHHCCCCHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
WSTPEEMRGSWAGAMGHTQWMPEVWLNVGFDYDGDGKISPFGKPDDALGSTARYLVNRGK
CCCHHHHCCCHHCCCCCCHHCHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
YHRGEHWGYEVRAPGNMSGSRTYAAWQAAGVTRADGEPFPQPNASAQLWIPVEGGPAFLL
CCCCCCCCEEEECCCCCCCCCEEHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEE
GPNFYSVKSYNPAMSYALAICHLGDRILGAPPFLQPFPGSERALTLAEVQEMQKRLTAAG
CCCEEEECCCCHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHC
FATGGTDGRVGNDTMKAIRDFQAKAGLTPADGYGGLKVLTKLRQGG
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TQPDSPTRRTLLRSGLGAAALLVPAVPALAAPAEFDAWRDGFRARALAKGISQATWTRV
CCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MARLEPDMTVFRELRNQPEFKEQLWQYVNRRVSDWRVINGKVALKQNEALFARIEKDFGV
HHHCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEECCEEEEECCCHHHHHHHHHHCC
ERGTLLALWGVESAYGDPLVQQNHMRPVFPALAALAWNEPRRKAYWETELTNALKIVDRG
CCCCEEEEECCCHHHCCCCHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
WSTPEEMRGSWAGAMGHTQWMPEVWLNVGFDYDGDGKISPFGKPDDALGSTARYLVNRGK
CCCHHHHCCCHHCCCCCCHHCHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
YHRGEHWGYEVRAPGNMSGSRTYAAWQAAGVTRADGEPFPQPNASAQLWIPVEGGPAFLL
CCCCCCCCEEEECCCCCCCCCEEHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEE
GPNFYSVKSYNPAMSYALAICHLGDRILGAPPFLQPFPGSERALTLAEVQEMQKRLTAAG
CCCEEEECCCCHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHC
FATGGTDGRVGNDTMKAIRDFQAKAGLTPADGYGGLKVLTKLRQGG
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]