Click here to switch to the map view.

The map label for this gene is aes [H]

Identifier: 146340453

GI number: 146340453

Start: 3676216

End: 3677223

Strand: Reverse

Name: aes [H]

Synonym: BRADO3493

Alternate gene names: 146340453

Gene position: 3677223-3676216 (Counterclockwise)

Preceding gene: 146340454

Following gene: 146340452

Centisome position: 49.32

GC content: 67.16

Gene sequence:

>1008_bases
GTGCTTCAACTCGGAAGATCTCTTCAACGCAACAGGTTCTCTGCAATGCCCGTTCAACTCGATGCCGATGCCGCTGCCGT
CTACAAAGCGTTCCAGGAGGCCGGCCGTCCGGCTTACGAGACGCTGACACCGCAGGAGGCGCGCGACTACTATCTGGCCG
CCCGCACCGTCAGCAATCCCGAGCCGCCAGAGCTCGCCGAAGTCAAGGAGCTGGCGATCTCCGGCCCGCACGGCGCGATC
CCTGCCCGGCTCTATCGGCCGAAGTCGGTGCGGCAGACCAACGGGCTCGCCCCGGGCCTGGTGTTCCTCCATGGCGGCGG
CTGGGTGATCGGCAATCTCGAGTCGCATGACGTGGTCTGCCGCACACTGGCGCACGAAGGCGAGCTGATCGTCGTCTCGG
TCGACTATCGCCTCGCGCCCGAGCACAAGTTTCCCGCCGCGGTCGACGATGCGGTCGCTGCGACCCAATGGGTGGCCGGC
AACGCCGCCTCGCTCGGCATCGATGCGGCGAGGCTCAGCGTCGGCGGCGATTCCGCCGGGGGCAATCTCGCCGCCGTTGT
CGCGTTGTCCGCGCGTGACGGCAACGGCCCGAAGCTGTCTGGCCAGGTGCTGATCTATCCAGCCACTGACTTCACGATGA
GCAATCCCTCGCACAGCGAGCCCGAGACCAGCGTGCTGCTGACGCATTCGGTGATCCGCTGGTTTCGCGACCATTATCTC
AATAGCGCCGCCGACATCCACGACTGGCGCGCCTCGCCCGCCAAGGCCGAGTCTCTCGTGGGCCTGCCGCCCGCCTATGT
GCTGACCGCCGGCGCCGACCCGCTGCGCGATGAAGGCGACGACTACGCGCGGCGCTTGCGCGAGGCCGGCGTGCCCGTGA
CCTATCGCACTCATCCCGGCCAATTCCACGGCTTCTTCACCATGGGCAAGCTGCTCGACCAGGCCAATGTCGCCGCCCGC
GACATTGGCGCCTGGTTGAAGCAGCTGGATCGGCCGGTGGGCATCTAG

Upstream 100 bases:

>100_bases
GCTCGGAGGTCGCGGCGATCGTCGCCTTCCTCGCGTCAGATGATGCGAGCTACGTCTCCGGCGCCGCCTACACGGTCGAC
GGCGGCCGCACCGCGGCCTA

Downstream 100 bases:

>100_bases
GGCTGTTTTGGCCCGTGCTGATCGGCTCTGCGCTGGCTGCGGCGCATTCCCGGCACGCAGCCGAGCATGGCCGAAATTCC
CGCTGTTTTTGCGCCGCAAC

Product: putative lipase/esterase

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MLQLGRSLQRNRFSAMPVQLDADAAAVYKAFQEAGRPAYETLTPQEARDYYLAARTVSNPEPPELAEVKELAISGPHGAI
PARLYRPKSVRQTNGLAPGLVFLHGGGWVIGNLESHDVVCRTLAHEGELIVVSVDYRLAPEHKFPAAVDDAVAATQWVAG
NAASLGIDAARLSVGGDSAGGNLAAVVALSARDGNGPKLSGQVLIYPATDFTMSNPSHSEPETSVLLTHSVIRWFRDHYL
NSAADIHDWRASPAKAESLVGLPPAYVLTAGADPLRDEGDDYARRLREAGVPVTYRTHPGQFHGFFTMGKLLDQANVAAR
DIGAWLKQLDRPVGI

Sequences:

>Translated_335_residues
MLQLGRSLQRNRFSAMPVQLDADAAAVYKAFQEAGRPAYETLTPQEARDYYLAARTVSNPEPPELAEVKELAISGPHGAI
PARLYRPKSVRQTNGLAPGLVFLHGGGWVIGNLESHDVVCRTLAHEGELIVVSVDYRLAPEHKFPAAVDDAVAATQWVAG
NAASLGIDAARLSVGGDSAGGNLAAVVALSARDGNGPKLSGQVLIYPATDFTMSNPSHSEPETSVLLTHSVIRWFRDHYL
NSAADIHDWRASPAKAESLVGLPPAYVLTAGADPLRDEGDDYARRLREAGVPVTYRTHPGQFHGFFTMGKLLDQANVAAR
DIGAWLKQLDRPVGI
>Mature_335_residues
MLQLGRSLQRNRFSAMPVQLDADAAAVYKAFQEAGRPAYETLTPQEARDYYLAARTVSNPEPPELAEVKELAISGPHGAI
PARLYRPKSVRQTNGLAPGLVFLHGGGWVIGNLESHDVVCRTLAHEGELIVVSVDYRLAPEHKFPAAVDDAVAATQWVAG
NAASLGIDAARLSVGGDSAGGNLAAVVALSARDGNGPKLSGQVLIYPATDFTMSNPSHSEPETSVLLTHSVIRWFRDHYL
NSAADIHDWRASPAKAESLVGLPPAYVLTAGADPLRDEGDDYARRLREAGVPVTYRTHPGQFHGFFTMGKLLDQANVAAR
DIGAWLKQLDRPVGI

Specific function: Displays esterase activity towards short chain fatty esters (acyl chain length of up to 8 carbons). Able to hydrolyze triacetylglycerol (triacetin) and tributyrylglycerol (tributyrin), but not trioleylglycerol (triolein) or cholesterol oleate. Negatively

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI157041239, Length=286, Percent_Identity=31.1188811188811, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI206597554, Length=286, Percent_Identity=31.1188811188811, Blast_Score=114, Evalue=8e-26,
Organism=Homo sapiens, GI68299767, Length=312, Percent_Identity=29.1666666666667, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI61966717, Length=288, Percent_Identity=27.7777777777778, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI68051721, Length=304, Percent_Identity=25.9868421052632, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI157041237, Length=251, Percent_Identity=28.6852589641434, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI226423947, Length=312, Percent_Identity=25.6410256410256, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI21328446, Length=178, Percent_Identity=33.7078651685393, Blast_Score=79, Evalue=4e-15,
Organism=Escherichia coli, GI1786682, Length=308, Percent_Identity=29.2207792207792, Blast_Score=126, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17567059, Length=302, Percent_Identity=33.7748344370861, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71996133, Length=131, Percent_Identity=41.2213740458015, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17540028, Length=130, Percent_Identity=39.2307692307692, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI72001146, Length=145, Percent_Identity=37.9310344827586, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI115533410, Length=108, Percent_Identity=36.1111111111111, Blast_Score=69, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI115533412, Length=108, Percent_Identity=36.1111111111111, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24656084, Length=96, Percent_Identity=40.625, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24656076, Length=96, Percent_Identity=40.625, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI20130169, Length=96, Percent_Identity=40.625, Blast_Score=72, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 35969; Mature: 35969

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQLGRSLQRNRFSAMPVQLDADAAAVYKAFQEAGRPAYETLTPQEARDYYLAARTVSNP
CCCCCCHHHHCCCCCCCEEECCHHHHHHHHHHHHCCCHHHCCCCHHHHCEEEEEEECCCC
EPPELAEVKELAISGPHGAIPARLYRPKSVRQTNGLAPGLVFLHGGGWVIGNLESHDVVC
CCCHHHHHHHHHCCCCCCCCCHHHCCCCHHHHHCCCCCCEEEEECCCEEEECCCCCHHHH
RTLAHEGELIVVSVDYRLAPEHKFPAAVDDAVAATQWVAGNAASLGIDAARLSVGGDSAG
HHHHCCCCEEEEEECCEECCCCCCCCHHHHHHHHHHHHCCCCHHCCCCEEEEEECCCCCC
GNLAAVVALSARDGNGPKLSGQVLIYPATDFTMSNPSHSEPETSVLLTHSVIRWFRDHYL
CCEEEEEEEECCCCCCCEECCEEEEEECCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHC
NSAADIHDWRASPAKAESLVGLPPAYVLTAGADPLRDEGDDYARRLREAGVPVTYRTHPG
CCCCHHHHHCCCCHHHHHHCCCCCCEEEECCCCCCCCCCHHHHHHHHHCCCCEEEECCCC
QFHGFFTMGKLLDQANVAARDIGAWLKQLDRPVGI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLQLGRSLQRNRFSAMPVQLDADAAAVYKAFQEAGRPAYETLTPQEARDYYLAARTVSNP
CCCCCCHHHHCCCCCCCEEECCHHHHHHHHHHHHCCCHHHCCCCHHHHCEEEEEEECCCC
EPPELAEVKELAISGPHGAIPARLYRPKSVRQTNGLAPGLVFLHGGGWVIGNLESHDVVC
CCCHHHHHHHHHCCCCCCCCCHHHCCCCHHHHHCCCCCCEEEEECCCEEEECCCCCHHHH
RTLAHEGELIVVSVDYRLAPEHKFPAAVDDAVAATQWVAGNAASLGIDAARLSVGGDSAG
HHHHCCCCEEEEEECCEECCCCCCCCHHHHHHHHHHHHCCCCHHCCCCEEEEEECCCCCC
GNLAAVVALSARDGNGPKLSGQVLIYPATDFTMSNPSHSEPETSVLLTHSVIRWFRDHYL
CCEEEEEEEECCCCCCCEECCEEEEEECCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHC
NSAADIHDWRASPAKAESLVGLPPAYVLTAGADPLRDEGDDYARRLREAGVPVTYRTHPG
CCCCHHHHHCCCCHHHHHHCCCCCCEEEECCCCCCCCCCHHHHHHHHHCCCCEEEECCCC
QFHGFFTMGKLLDQANVAARDIGAWLKQLDRPVGI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA