BacMap

Definition	Bradyrhizobium sp. ORS278 chromosome, complete genome.
Accession	NC_009445
Length	7,456,587

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The map label for this gene is braF [H]

Identifier: 146340377

GI number: 146340377

Start: 3597904

End: 3598818

Strand: Direct

Name: braF [H]

Synonym: BRADO3406

Alternate gene names: 146340377

Gene position: 3597904-3598818 (Clockwise)

Preceding gene: 146340376

Following gene: 146340378

Centisome position: 48.25

GC content: 67.98

Gene sequence:

>915_bases
ATGATGTTGAGCGGGCTGACCACTCCGCTGGACGTGCTGGCGCTGGAGACCTGGGCTCCAGGGACGGCGCGTCAGGTGCT
CGCGGCAGAGCCGGCCGACGTGCCGACGTCGGCCGCGGGCGATGCGCGCACGGTGCTGCGGCTCGCCAACATCTCGCTGT
CATTCGGCGGTCTCGCTGCCCTCCGCAATATCGATCTCGATGTCGCAGAGGGCGAGATCCGCGCTGTGATCGGTCCGAAC
GGCGCCGGAAAGAGTTCGCTGCTCAACGTCGTCAGCGGGCTCTACCGGCCGGAGCAAGGGGAGGTCTGGTTCGGCGATCA
TGCGTTCCGGCAGGTGCCGACCGCGCAGCTCGCGCGCCATGGCGTCGCCCGCACCTTTCAGAACCTTGCTTTATTCAAGG
GGCTTTCCGTGTTCGACAATGTGCTGATGGGGCTGGCGCACCGCGTGCGCGCCGGCTTCATCCGCCAGGCCGTCGGCACG
CCCTTGGCGCGGCACATCGAGGCCGAGAACCGCGCCGCCGCGGAGGAGGTCGTTGGCTTCCTGCATCTGCGGGAGGCGCG
GGACCGTATCGCCGGAACGTTGCCCTACGGCCTGCAGAAGCGCGTCGAGCTGGCGCGCGCGCTGGTGGCGCGTCCAAGAC
TGCTGCTGCTGGACGAGCCGTTCGCCGGCGTCACCTTAAGCGAGAAGCAGGAGCTGGCGCAGCACGTGCGCGGCGCGCGC
GACCGCTACGGCACCACCATCGTGCTGATCGAGCACGACATCGGCATCGTCATGGATCTGTCCGACCACATCGCCGTGCT
CGACTACGGCCGCAAGATCGCCGACGGACCGCCGGCCGAGATCAAGACCAACCAGGCGGTGATCGATGCCTATCTCGGCG
TCGCCCATGAAGACGAGACGGAGCTCGCGATCTGA

Upstream 100 bases:

>100_bases
TATTTCGGCAAGCACGTGATCCCGCTGGTGCGCGAGCTCGAAGCCAAGGCCGCGGCCAAGGCGGGCGTGCAAGTGCAGGC
GGCGGAGTAGGGCGAGCGCG

Downstream 100 bases:

>100_bases
TGTTCGACTATCAGTTTCTCATCGAGGTGCTGGTCGGCGGCCTGCTCTCCGGGGTGATGTATTCGCTGGTCGCGATCGGC
TTCGTGCTGATCTACAAGAC

Product: putative branched-chain amino acid ABC transporter ATP-binding protein

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MMLSGLTTPLDVLALETWAPGTARQVLAAEPADVPTSAAGDARTVLRLANISLSFGGLAALRNIDLDVAEGEIRAVIGPN
GAGKSSLLNVVSGLYRPEQGEVWFGDHAFRQVPTAQLARHGVARTFQNLALFKGLSVFDNVLMGLAHRVRAGFIRQAVGT
PLARHIEAENRAAAEEVVGFLHLREARDRIAGTLPYGLQKRVELARALVARPRLLLLDEPFAGVTLSEKQELAQHVRGAR
DRYGTTIVLIEHDIGIVMDLSDHIAVLDYGRKIADGPPAEIKTNQAVIDAYLGVAHEDETELAI

Sequences:

>Translated_304_residues
MMLSGLTTPLDVLALETWAPGTARQVLAAEPADVPTSAAGDARTVLRLANISLSFGGLAALRNIDLDVAEGEIRAVIGPN
GAGKSSLLNVVSGLYRPEQGEVWFGDHAFRQVPTAQLARHGVARTFQNLALFKGLSVFDNVLMGLAHRVRAGFIRQAVGT
PLARHIEAENRAAAEEVVGFLHLREARDRIAGTLPYGLQKRVELARALVARPRLLLLDEPFAGVTLSEKQELAQHVRGAR
DRYGTTIVLIEHDIGIVMDLSDHIAVLDYGRKIADGPPAEIKTNQAVIDAYLGVAHEDETELAI
>Mature_304_residues
MMLSGLTTPLDVLALETWAPGTARQVLAAEPADVPTSAAGDARTVLRLANISLSFGGLAALRNIDLDVAEGEIRAVIGPN
GAGKSSLLNVVSGLYRPEQGEVWFGDHAFRQVPTAQLARHGVARTFQNLALFKGLSVFDNVLMGLAHRVRAGFIRQAVGT
PLARHIEAENRAAAEEVVGFLHLREARDRIAGTLPYGLQKRVELARALVARPRLLLLDEPFAGVTLSEKQELAQHVRGAR
DRYGTTIVLIEHDIGIVMDLSDHIAVLDYGRKIADGPPAEIKTNQAVIDAYLGVAHEDETELAI

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0411

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI27881501, Length=263, Percent_Identity=25.4752851711027, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI30795238, Length=263, Percent_Identity=25.4752851711027, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI27477115, Length=194, Percent_Identity=27.319587628866, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI21536378, Length=230, Percent_Identity=27.3913043478261, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI153792144, Length=194, Percent_Identity=28.3505154639175, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI105990541, Length=227, Percent_Identity=27.3127753303965, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI27262624, Length=243, Percent_Identity=26.7489711934156, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI27262626, Length=243, Percent_Identity=26.7489711934156, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI27436953, Length=258, Percent_Identity=23.6434108527132, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI150417984, Length=219, Percent_Identity=29.2237442922374, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI21536376, Length=249, Percent_Identity=25.3012048192771, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI47078218, Length=256, Percent_Identity=24.21875, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI116734710, Length=224, Percent_Identity=26.3392857142857, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI45446740, Length=223, Percent_Identity=24.6636771300448, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI9665248, Length=217, Percent_Identity=26.2672811059908, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI31657092, Length=231, Percent_Identity=27.7056277056277, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1789864, Length=257, Percent_Identity=39.2996108949416, Blast_Score=193, Evalue=1e-50,
Organism=Escherichia coli, GI1790467, Length=236, Percent_Identity=32.2033898305085, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI87081791, Length=237, Percent_Identity=29.957805907173, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI87081709, Length=239, Percent_Identity=26.7782426778243, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1787370, Length=237, Percent_Identity=30.379746835443, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1789593, Length=248, Percent_Identity=29.8387096774194, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1788761, Length=238, Percent_Identity=32.7731092436975, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI48995001, Length=244, Percent_Identity=31.5573770491803, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1788450, Length=243, Percent_Identity=29.2181069958848, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1787712, Length=224, Percent_Identity=31.6964285714286, Blast_Score=104, Evalue=8e-24,
Organism=Escherichia coli, GI87082268, Length=251, Percent_Identity=31.4741035856574, Blast_Score=104, Evalue=9e-24,
Organism=Escherichia coli, GI1787029, Length=241, Percent_Identity=30.2904564315353, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1789991, Length=233, Percent_Identity=31.3304721030043, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1790190, Length=257, Percent_Identity=28.7937743190661, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1786398, Length=243, Percent_Identity=28.8065843621399, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87082267, Length=237, Percent_Identity=29.957805907173, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1788472, Length=254, Percent_Identity=27.5590551181102, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1788225, Length=247, Percent_Identity=29.5546558704453, Blast_Score=97, Evalue=9e-22,
Organism=Escherichia coli, GI1790525, Length=242, Percent_Identity=26.0330578512397, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1786253, Length=252, Percent_Identity=28.968253968254, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1790162, Length=251, Percent_Identity=27.8884462151394, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1789032, Length=242, Percent_Identity=28.9256198347107, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1790739, Length=238, Percent_Identity=28.9915966386555, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788897, Length=247, Percent_Identity=25.9109311740891, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1786803, Length=246, Percent_Identity=26.8292682926829, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1786563, Length=228, Percent_Identity=28.9473684210526, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1790544, Length=257, Percent_Identity=29.9610894941634, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI1787758, Length=231, Percent_Identity=28.1385281385281, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI48994943, Length=245, Percent_Identity=29.7959183673469, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI48994997, Length=234, Percent_Identity=26.9230769230769, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1789586, Length=245, Percent_Identity=28.5714285714286, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1789672, Length=241, Percent_Identity=24.896265560166, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI87081782, Length=248, Percent_Identity=27.0161290322581, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1787089, Length=238, Percent_Identity=31.0924369747899, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1786345, Length=248, Percent_Identity=27.4193548387097, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1788210, Length=252, Percent_Identity=27.7777777777778, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI1789873, Length=232, Percent_Identity=29.3103448275862, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788053, Length=230, Percent_Identity=29.1304347826087, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787792, Length=233, Percent_Identity=27.0386266094421, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787164, Length=228, Percent_Identity=29.8245614035088, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1787547, Length=265, Percent_Identity=24.5283018867925, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1789891, Length=220, Percent_Identity=29.0909090909091, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI87081834, Length=218, Percent_Identity=27.0642201834862, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1789962, Length=254, Percent_Identity=24.8031496062992, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786703, Length=200, Percent_Identity=29, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1786872, Length=235, Percent_Identity=26.3829787234043, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787500, Length=233, Percent_Identity=25.3218884120172, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788506, Length=282, Percent_Identity=23.0496453900709, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1787548, Length=252, Percent_Identity=25, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI48994883, Length=261, Percent_Identity=24.1379310344828, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1787105, Length=231, Percent_Identity=29.004329004329, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI145693107, Length=262, Percent_Identity=24.4274809160305, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1786698, Length=214, Percent_Identity=27.1028037383178, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787143, Length=239, Percent_Identity=26.3598326359833, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787759, Length=254, Percent_Identity=24.4094488188976, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788644, Length=262, Percent_Identity=24.8091603053435, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1786981, Length=223, Percent_Identity=26.9058295964126, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI115533608, Length=265, Percent_Identity=27.5471698113208, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI115534520, Length=254, Percent_Identity=26.3779527559055, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI212646699, Length=264, Percent_Identity=25, Blast_Score=77, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI25146777, Length=216, Percent_Identity=25.9259259259259, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI193211015, Length=227, Percent_Identity=25.9911894273128, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI193211017, Length=227, Percent_Identity=25.9911894273128, Blast_Score=70, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI71996809, Length=227, Percent_Identity=25.9911894273128, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71983523, Length=241, Percent_Identity=23.6514522821577, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71983516, Length=241, Percent_Identity=23.6514522821577, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71983510, Length=241, Percent_Identity=23.6514522821577, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17558664, Length=224, Percent_Identity=28.125, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17510237, Length=229, Percent_Identity=23.5807860262009, Blast_Score=68, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6322640, Length=249, Percent_Identity=24.4979919678715, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324985, Length=230, Percent_Identity=23.9130434782609, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI221512771, Length=231, Percent_Identity=27.2727272727273, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24666092, Length=263, Percent_Identity=25.8555133079848, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24581191, Length=221, Percent_Identity=26.6968325791855, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24581189, Length=221, Percent_Identity=26.6968325791855, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24581193, Length=221, Percent_Identity=26.6968325791855, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24581195, Length=221, Percent_Identity=26.6968325791855, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24659289, Length=234, Percent_Identity=23.9316239316239, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24648314, Length=237, Percent_Identity=24.0506329113924, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24643674, Length=225, Percent_Identity=21.7777777777778, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 32745; Mature: 32745

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMLSGLTTPLDVLALETWAPGTARQVLAAEPADVPTSAAGDARTVLRLANISLSFGGLAA
CCCCCCCCCHHHHEECCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHH
LRNIDLDVAEGEIRAVIGPNGAGKSSLLNVVSGLYRPEQGEVWFGDHAFRQVPTAQLARH
HHHCCCEECCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCEEECCHHHHHCCHHHHHHH
GVARTFQNLALFKGLSVFDNVLMGLAHRVRAGFIRQAVGTPLARHIEAENRAAAEEVVGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
LHLREARDRIAGTLPYGLQKRVELARALVARPRLLLLDEPFAGVTLSEKQELAQHVRGAR
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHH
DRYGTTIVLIEHDIGIVMDLSDHIAVLDYGRKIADGPPAEIKTNQAVIDAYLGVAHEDET
HHCCCEEEEEECCCEEEEECCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCC
ELAI
CCCC
>Mature Secondary Structure
MMLSGLTTPLDVLALETWAPGTARQVLAAEPADVPTSAAGDARTVLRLANISLSFGGLAA
CCCCCCCCCHHHHEECCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHH
LRNIDLDVAEGEIRAVIGPNGAGKSSLLNVVSGLYRPEQGEVWFGDHAFRQVPTAQLARH
HHHCCCEECCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCEEECCHHHHHCCHHHHHHH
GVARTFQNLALFKGLSVFDNVLMGLAHRVRAGFIRQAVGTPLARHIEAENRAAAEEVVGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
LHLREARDRIAGTLPYGLQKRVELARALVARPRLLLLDEPFAGVTLSEKQELAQHVRGAR
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHH
DRYGTTIVLIEHDIGIVMDLSDHIAVLDYGRKIADGPPAEIKTNQAVIDAYLGVAHEDET
HHCCCEEEEEECCCEEEEECCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCC
ELAI
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]