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The map label for this gene is thrS [H]

Identifier: 146340273

GI number: 146340273

Start: 3490388

End: 3491617

Strand: Direct

Name: thrS [H]

Synonym: BRADO3294

Alternate gene names: 146340273

Gene position: 3490388-3491617 (Clockwise)

Preceding gene: 146340271

Following gene: 146340274

Centisome position: 46.81

GC content: 68.37

Gene sequence:

>1230_bases
ATGGACGATCTCGATCACAGAAGCCTCGGACCCCGTCTCGATCTCTGGCACATCCAGGAGGATGCGCCGGGAATGGTGTT
CTGGCATCCGCGCGGCCACGCGCTCTATCGCGTGCTCGAAAACTTTATCCGCGGCAAGATGCGCCGGCTCGGCTATGCCG
AGGTCAGGACGCCGCAGCTGCTGCCGCTCGACTTGTGGATGCAAAGCGGGCATTGGGAGAAGTTCGGCGCGCAGATGTTC
GCCCTCGGCGAGGGCGAGCGCGCGATGGCGCTGAAGCCGATGTCGTGTCCCTGCCATGTCCAGATCTTCAACAGGAATTT
GAGGTCGTGGCGCGAGCTGCCGCTGCGCTACGCCGAGTTCGGCAGCTGCCATCGCGACGAGCCGTCTGGCGCGCTGCACG
GTCTGATGCGCACGCGCGGCTTCGAGCAGGACGATGCGCATGTGTTCTGCCGCGCCGACGATGTCGCGGGCGAGGTCGCC
CGCTTCGCCGCGCTGCTCTCGGAGGTCTATGCCGCGTTCGGCTTCGACGCGCCGGAGGTGTCGCTGTCGACGCGACCGCA
ACTGCGCGCCGGATCGGACGCGTCATGGGATTGGGCGGAGCAGGCTCTGGCCGAAGCCGCCCGCAGCTGTGGCCTGTCCT
ATGCGATCCAGCCCGGCGAGGGCGCGTTCTACGGACCGAAGCTGGAGTTTGCGCTGCGCGACCGGCTCGGCCGCTCCTGG
CAGTGCGGCACGGTTCAGCTCGACAGCGTGCTTCCGGAACGTCTCGGCGCCTCCTATGTCGCCGCCCACGGTGCCCGGGC
GACGCCGGTCCTGATCCACCACGCGGTGCTCGGCTCGATCGGTCGCTTCATCGCGATGCTGCTCGAGCACCACGCCGGCG
CGCTGCCGTTCTGGCTGTCGCCCGACCAGGTCGCGGTCGTGCCGATCACGAGGGATCAGCTGGACGATGCGTTGAAGCTC
CGCGAACTTCTCTTCAGCGCCGAGCTGCGCGCCGTTCTATCAGCTGATGGCGAGACGCTGTCTCGGCGCATCGTCGCGGC
GCATGCGGCGAGCATTCCCGTCGTTGCGGTGGTCGGGGCTCGTGAAGCAGCGCAGGGCACCGTGAGCCTGCGCGAGCGCG
ACGGGACGACCTCCGTGCTCACGTGGGATCGGGCGGTCAGCACGCTGCGCGAGCGGAATCGGCCGCCTCGCGGTAGCGTC
GATCGCGCGTGCGGCCCCGATCTTGACTGA

Upstream 100 bases:

>100_bases
AGATCGCATTCCCAGAACAGGCCCCGCCGGCGACGGACGGGGCCTTTTCTGTGTCTGCACAAACCCTCGTCCGCGCGGCT
TCATGCAAAGGAAGCTAGTC

Downstream 100 bases:

>100_bases
ACCAGTCAGTGCCGCGGCGCGACCAATACGACGCGCCGCAGGCGCGTTGGGGCAGGGCGCTCACCGCGACGGCCGAAGAG
CAAGAGGGAGGATCTATGAG

Product: putative threonine tRNA synthetase (fragment)

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MDDLDHRSLGPRLDLWHIQEDAPGMVFWHPRGHALYRVLENFIRGKMRRLGYAEVRTPQLLPLDLWMQSGHWEKFGAQMF
ALGEGERAMALKPMSCPCHVQIFNRNLRSWRELPLRYAEFGSCHRDEPSGALHGLMRTRGFEQDDAHVFCRADDVAGEVA
RFAALLSEVYAAFGFDAPEVSLSTRPQLRAGSDASWDWAEQALAEAARSCGLSYAIQPGEGAFYGPKLEFALRDRLGRSW
QCGTVQLDSVLPERLGASYVAAHGARATPVLIHHAVLGSIGRFIAMLLEHHAGALPFWLSPDQVAVVPITRDQLDDALKL
RELLFSAELRAVLSADGETLSRRIVAAHAASIPVVAVVGAREAAQGTVSLRERDGTTSVLTWDRAVSTLRERNRPPRGSV
DRACGPDLD

Sequences:

>Translated_409_residues
MDDLDHRSLGPRLDLWHIQEDAPGMVFWHPRGHALYRVLENFIRGKMRRLGYAEVRTPQLLPLDLWMQSGHWEKFGAQMF
ALGEGERAMALKPMSCPCHVQIFNRNLRSWRELPLRYAEFGSCHRDEPSGALHGLMRTRGFEQDDAHVFCRADDVAGEVA
RFAALLSEVYAAFGFDAPEVSLSTRPQLRAGSDASWDWAEQALAEAARSCGLSYAIQPGEGAFYGPKLEFALRDRLGRSW
QCGTVQLDSVLPERLGASYVAAHGARATPVLIHHAVLGSIGRFIAMLLEHHAGALPFWLSPDQVAVVPITRDQLDDALKL
RELLFSAELRAVLSADGETLSRRIVAAHAASIPVVAVVGAREAAQGTVSLRERDGTTSVLTWDRAVSTLRERNRPPRGSV
DRACGPDLD
>Mature_409_residues
MDDLDHRSLGPRLDLWHIQEDAPGMVFWHPRGHALYRVLENFIRGKMRRLGYAEVRTPQLLPLDLWMQSGHWEKFGAQMF
ALGEGERAMALKPMSCPCHVQIFNRNLRSWRELPLRYAEFGSCHRDEPSGALHGLMRTRGFEQDDAHVFCRADDVAGEVA
RFAALLSEVYAAFGFDAPEVSLSTRPQLRAGSDASWDWAEQALAEAARSCGLSYAIQPGEGAFYGPKLEFALRDRLGRSW
QCGTVQLDSVLPERLGASYVAAHGARATPVLIHHAVLGSIGRFIAMLLEHHAGALPFWLSPDQVAVVPITRDQLDDALKL
RELLFSAELRAVLSADGETLSRRIVAAHAASIPVVAVVGAREAAQGTVSLRERDGTTSVLTWDRAVSTLRERNRPPRGSV
DRACGPDLD

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI38202255, Length=390, Percent_Identity=41.7948717948718, Blast_Score=319, Evalue=3e-87,
Organism=Homo sapiens, GI20070344, Length=389, Percent_Identity=43.9588688946015, Blast_Score=302, Evalue=4e-82,
Organism=Homo sapiens, GI38570111, Length=373, Percent_Identity=39.6782841823056, Blast_Score=300, Evalue=1e-81,
Organism=Escherichia coli, GI1788013, Length=371, Percent_Identity=48.5175202156334, Blast_Score=378, Evalue=1e-106,
Organism=Escherichia coli, GI1786392, Length=162, Percent_Identity=31.4814814814815, Blast_Score=88, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71984184, Length=389, Percent_Identity=39.3316195372751, Blast_Score=316, Evalue=2e-86,
Organism=Caenorhabditis elegans, GI71984192, Length=389, Percent_Identity=39.3316195372751, Blast_Score=315, Evalue=2e-86,
Organism=Saccharomyces cerevisiae, GI6322112, Length=402, Percent_Identity=38.3084577114428, Blast_Score=301, Evalue=2e-82,
Organism=Saccharomyces cerevisiae, GI6322654, Length=397, Percent_Identity=35.7682619647355, Blast_Score=250, Evalue=3e-67,
Organism=Drosophila melanogaster, GI24583842, Length=372, Percent_Identity=43.5483870967742, Blast_Score=325, Evalue=5e-89,
Organism=Drosophila melanogaster, GI28574141, Length=372, Percent_Identity=43.5483870967742, Blast_Score=325, Evalue=5e-89,
Organism=Drosophila melanogaster, GI24583839, Length=372, Percent_Identity=43.5483870967742, Blast_Score=324, Evalue=6e-89,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 45285; Mature: 45285

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDLDHRSLGPRLDLWHIQEDAPGMVFWHPRGHALYRVLENFIRGKMRRLGYAEVRTPQL
CCCCCCCCCCCCCEEEEECCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCHHHCCCCCE
LPLDLWMQSGHWEKFGAQMFALGEGERAMALKPMSCPCHVQIFNRNLRSWRELPLRYAEF
EEHHHHHCCCCHHHHCCEEEEECCCCCEEEECCCCCCEEEHHHHHHHHHHHHCCHHHHHH
GSCHRDEPSGALHGLMRTRGFEQDDAHVFCRADDVAGEVARFAALLSEVYAAFGFDAPEV
CCCCCCCCCHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCC
SLSTRPQLRAGSDASWDWAEQALAEAARSCGLSYAIQPGEGAFYGPKLEFALRDRLGRSW
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCEECCCHHHHHHHHCCCCC
QCGTVQLDSVLPERLGASYVAAHGARATPVLIHHAVLGSIGRFIAMLLEHHAGALPFWLS
CCCCEEHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEC
PDQVAVVPITRDQLDDALKLRELLFSAELRAVLSADGETLSRRIVAAHAASIPVVAVVGA
CCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEECC
REAAQGTVSLRERDGTTSVLTWDRAVSTLRERNRPPRGSVDRACGPDLD
CHHCCCCEEEECCCCCCEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDDLDHRSLGPRLDLWHIQEDAPGMVFWHPRGHALYRVLENFIRGKMRRLGYAEVRTPQL
CCCCCCCCCCCCCEEEEECCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCHHHCCCCCE
LPLDLWMQSGHWEKFGAQMFALGEGERAMALKPMSCPCHVQIFNRNLRSWRELPLRYAEF
EEHHHHHCCCCHHHHCCEEEEECCCCCEEEECCCCCCEEEHHHHHHHHHHHHCCHHHHHH
GSCHRDEPSGALHGLMRTRGFEQDDAHVFCRADDVAGEVARFAALLSEVYAAFGFDAPEV
CCCCCCCCCHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCC
SLSTRPQLRAGSDASWDWAEQALAEAARSCGLSYAIQPGEGAFYGPKLEFALRDRLGRSW
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCEECCCHHHHHHHHCCCCC
QCGTVQLDSVLPERLGASYVAAHGARATPVLIHHAVLGSIGRFIAMLLEHHAGALPFWLS
CCCCEEHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEC
PDQVAVVPITRDQLDDALKLRELLFSAELRAVLSADGETLSRRIVAAHAASIPVVAVVGA
CCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEECC
REAAQGTVSLRERDGTTSVLTWDRAVSTLRERNRPPRGSVDRACGPDLD
CHHCCCCEEEECCCCCCEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA