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The map label for this gene is trmJ [H]

Identifier: 146339760

GI number: 146339760

Start: 2967372

End: 2968571

Strand: Direct

Name: trmJ [H]

Synonym: BRADO2760

Alternate gene names: 146339760

Gene position: 2967372-2968571 (Clockwise)

Preceding gene: 146339756

Following gene: 146339762

Centisome position: 39.8

GC content: 69.83

Gene sequence:

>1200_bases
GTGTCGGGCACCGACAAGACCAAGGCAGGCGTGGAGATCGCAGGACCGGTGGTGATCCTGGTCGAGCCGCAGCTCGGCGA
GAACATCGGCATGGCCGCGCGCGCCATGGGCAATTTCGCGCTGACCGAGCTGCGCCTGGTCAATCCCCGCGACGGCTGGC
CGAACATCGCGGCCCAACGGGCGGCGGCCGGCGCCGACCACATCATCGACCACGTCCGGTTGTTCGAGACCCTGCCGGAG
GCGATCGCCGACCTCGACCTGGTGTTCGCGACGACAGCCCGCTCCCACGATCAGGCCAAGCCGGTGATGGGGCCGGAGGC
GGCGGCGCGGGAGATGCAGGGCCAGATCGCCAGTGGCGGCCGCGCCGGCATCCTGTTCGGCCGTGAGCGGTCGGGCCTGA
CCAATGAGGAGGTCGCCCGCGCCAACCGGATCATCACCTATCCGGTCAATCCGGGCTTCGCCTCGCTGAACCTCGCCCAG
GCCGTGCTGCTGATGGGCTATGAATGGTTCAAGCTGGTGACCTCGGGCGCGTTGCCGTTCGCGATGCCCGAGCGCTCGGA
GCGCGCGACCCAGGAGCAGATGGAGGCGTTCTTCGAGAACCTCGTCCGCGAGCTCGACCGTGTGGAATTCCTGCGCCCGG
CCGAGAAGCGCGAGACCATGCTGGTGAACCTGCGCAACATCTTCTTCCGCATGGAGCCGACGAAGCAGGACATGCACACG
CTGCACGGCGTGGTCATGGCGATCGCCGAGGGGCGCAAGGGCCCGGCCAAGGGCGGCGTGCTCGACAGCACCCAGGCGAC
GCGGCTGCGCGCGCTGATCGCCGAGCAGGGCACCGTCGCCAACCTGCCGGACTCGCGCGGCTCGCTGCGCGGCCTGGCGC
GGCTGCTCCGCCGCAACCCGACCGACGCCGAGCGCGCGCTGTGGCACGCGCTGTCGCGCGACCGCCGCTTCGCCGGCCAG
TTCAAGCGCCAGACCCCGGTCGGCCGCCATATCCCGGACTTCGTCTCCTTCATCCACCGCCTGGCCATCGAGCTGGTCGG
CACTGATGAGAGCAAAGGCGTGGTCGCCGATCGCGCCCAGCGCAAGGCGTGGCTGGAGAGCCGCGACTATCGCGTGACCG
AGGTGAAGGCGGCGGATGTCGAGGCCAATCTGGAGGCCGAGCTGGCCCGGCTGCACGGGATGATTGCCGAGACCAAGTAG

Upstream 100 bases:

>100_bases
CGATTATTTTGCTTGAGAATTCTCGCCAGCCGGGCCAACGGAGCGGGCGGGGCGCGCGCATGTTCGCGCCTCACGCGACA
TAGAAGGTGAGGACAGTCCC

Downstream 100 bases:

>100_bases
CTGATCACCGCGCATGCCGCGTGACGTTGTCGGGCCGGATCATGCTTGATATCCTTGATCGGAGGTCAGGAAGGGCAGAC
TGTGGCAAAGCTTGCGCGGC

Product: putative tRNA/rRNA methyltransferase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MSGTDKTKAGVEIAGPVVILVEPQLGENIGMAARAMGNFALTELRLVNPRDGWPNIAAQRAAAGADHIIDHVRLFETLPE
AIADLDLVFATTARSHDQAKPVMGPEAAAREMQGQIASGGRAGILFGRERSGLTNEEVARANRIITYPVNPGFASLNLAQ
AVLLMGYEWFKLVTSGALPFAMPERSERATQEQMEAFFENLVRELDRVEFLRPAEKRETMLVNLRNIFFRMEPTKQDMHT
LHGVVMAIAEGRKGPAKGGVLDSTQATRLRALIAEQGTVANLPDSRGSLRGLARLLRRNPTDAERALWHALSRDRRFAGQ
FKRQTPVGRHIPDFVSFIHRLAIELVGTDESKGVVADRAQRKAWLESRDYRVTEVKAADVEANLEAELARLHGMIAETK

Sequences:

>Translated_399_residues
MSGTDKTKAGVEIAGPVVILVEPQLGENIGMAARAMGNFALTELRLVNPRDGWPNIAAQRAAAGADHIIDHVRLFETLPE
AIADLDLVFATTARSHDQAKPVMGPEAAAREMQGQIASGGRAGILFGRERSGLTNEEVARANRIITYPVNPGFASLNLAQ
AVLLMGYEWFKLVTSGALPFAMPERSERATQEQMEAFFENLVRELDRVEFLRPAEKRETMLVNLRNIFFRMEPTKQDMHT
LHGVVMAIAEGRKGPAKGGVLDSTQATRLRALIAEQGTVANLPDSRGSLRGLARLLRRNPTDAERALWHALSRDRRFAGQ
FKRQTPVGRHIPDFVSFIHRLAIELVGTDESKGVVADRAQRKAWLESRDYRVTEVKAADVEANLEAELARLHGMIAETK
>Mature_398_residues
SGTDKTKAGVEIAGPVVILVEPQLGENIGMAARAMGNFALTELRLVNPRDGWPNIAAQRAAAGADHIIDHVRLFETLPEA
IADLDLVFATTARSHDQAKPVMGPEAAAREMQGQIASGGRAGILFGRERSGLTNEEVARANRIITYPVNPGFASLNLAQA
VLLMGYEWFKLVTSGALPFAMPERSERATQEQMEAFFENLVRELDRVEFLRPAEKRETMLVNLRNIFFRMEPTKQDMHTL
HGVVMAIAEGRKGPAKGGVLDSTQATRLRALIAEQGTVANLPDSRGSLRGLARLLRRNPTDAERALWHALSRDRRFAGQF
KRQTPVGRHIPDFVSFIHRLAIELVGTDESKGVVADRAQRKAWLESRDYRVTEVKAADVEANLEAELARLHGMIAETK

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=241, Percent_Identity=33.195020746888, Blast_Score=115, Evalue=6e-27,
Organism=Escherichia coli, GI1790865, Length=183, Percent_Identity=36.0655737704918, Blast_Score=104, Evalue=8e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 44009; Mature: 43878

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGTDKTKAGVEIAGPVVILVEPQLGENIGMAARAMGNFALTELRLVNPRDGWPNIAAQR
CCCCCCCCCCEEEECCEEEEECCCCCCCCCHHHHHHCCHHEEEEEEECCCCCCCCHHHHH
AAAGADHIIDHVRLFETLPEAIADLDLVFATTARSHDQAKPVMGPEAAAREMQGQIASGG
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCC
RAGILFGRERSGLTNEEVARANRIITYPVNPGFASLNLAQAVLLMGYEWFKLVTSGALPF
CEEEEEECCCCCCCHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
AMPERSERATQEQMEAFFENLVRELDRVEFLRPAEKRETMLVNLRNIFFRMEPTKQDMHT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEECCCCHHHHHH
LHGVVMAIAEGRKGPAKGGVLDSTQATRLRALIAEQGTVANLPDSRGSLRGLARLLRRNP
HHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCC
TDAERALWHALSRDRRFAGQFKRQTPVGRHIPDFVSFIHRLAIELVGTDESKGVVADRAQ
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
RKAWLESRDYRVTEVKAADVEANLEAELARLHGMIAETK
HHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SGTDKTKAGVEIAGPVVILVEPQLGENIGMAARAMGNFALTELRLVNPRDGWPNIAAQR
CCCCCCCCCEEEECCEEEEECCCCCCCCCHHHHHHCCHHEEEEEEECCCCCCCCHHHHH
AAAGADHIIDHVRLFETLPEAIADLDLVFATTARSHDQAKPVMGPEAAAREMQGQIASGG
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCC
RAGILFGRERSGLTNEEVARANRIITYPVNPGFASLNLAQAVLLMGYEWFKLVTSGALPF
CEEEEEECCCCCCCHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
AMPERSERATQEQMEAFFENLVRELDRVEFLRPAEKRETMLVNLRNIFFRMEPTKQDMHT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEECCCCHHHHHH
LHGVVMAIAEGRKGPAKGGVLDSTQATRLRALIAEQGTVANLPDSRGSLRGLARLLRRNP
HHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCC
TDAERALWHALSRDRRFAGQFKRQTPVGRHIPDFVSFIHRLAIELVGTDESKGVVADRAQ
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
RKAWLESRDYRVTEVKAADVEANLEAELARLHGMIAETK
HHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA