| Definition | Bradyrhizobium sp. ORS278 chromosome, complete genome. |
|---|---|
| Accession | NC_009445 |
| Length | 7,456,587 |
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The map label for this gene is mrcB [H]
Identifier: 146339720
GI number: 146339720
Start: 2914984
End: 2917263
Strand: Reverse
Name: mrcB [H]
Synonym: BRADO2718
Alternate gene names: 146339720
Gene position: 2917263-2914984 (Counterclockwise)
Preceding gene: 146339723
Following gene: 146339719
Centisome position: 39.12
GC content: 67.24
Gene sequence:
>2280_bases GTGCGGCAGATCATTCCAGACGATTGGAAGAAGCGGATCAACAATTTCCGCCTGGATCTCGACGCGCGTTTCGATTCGGC GCTGTTCTCCTCTCTGCGCGGCACCCGCGAGCTGTATGAGCGCTTCTCGGCATTCATGGACCGCTTCTACGTCGGCGGCT GGAAGCGCTGGGTGTTCGTCGAGCCGCTGTCGGAAGCCGCCACGATCGGCCTCGGCGGCCTGGTGCTGATGCTGGCGCTC GCCATCCCGGCGTTCCGCGAAACCGCCGACGAGGACTGGCTGAAGAAATCCGACCTCGCCGTCACCTTCCTCGACCGCTA CGGCAACCCGATCGGCAGCCGCGGCATCAAGCACAATGACTCGATCCCGCTGGAAGACTTCCCGGACAATCTGATCAAGG CGACGCTCGCGACCGAGGACCGCCGCTTCTACGACCATTTCGGCATCGACTTCCCCGGCCTTGCGCGCGCGCTCGTCACC AATGCCCAGGCCGGCGGCGTCCGCCAGGGTGGCTCCTCGATCAGCCAGCAGCTCGCCAAAAACCTGTTCCTGTCGAACGA GCGCACCATCGAGCGCAAGGTCAACGAGGCGTTCCTGGCGATCTGGCTGGAAACCCGGCTGACCAAGAACGAGATCCTGA AGCTCTATCTCGACCGCGCCTATATGGGCGGCGGCACTTTCGGCGTCGACGGCGCGGCGCATTTCTACTTCAACAAGTCG GTGCGCGACATCAACCTGGCGGAAGCCGCGATGCTGGCCGGCCTGTTCAAGGCGCCGACCAAGTTCGCGCCCCACATCAA CCTGCCCGCGGCGCGCGCCCGCGCCAACGTCGTGCTCGACAACCTCGTCGATGCCGGCTTCATGACCGAGGGCCAGGTGT TCGGCGCCCGCCGCAACCCCGCCTTCGCAGTCGACCGCCGCGACGAGAGCTCGCCGAACTATTTCCTCGACTATGCCTTC GACGAGATGCGCAAGCTGGTCGACACGTTCCCGAAATCCTACACCGAGCGCGTGTTCGTGGTGCGGACGTCGATCGACAT GAACGTGCAGCACGCCGCCGACGAGGCGATCGAGAACCAGCTGCGCCAGTTCGGCCGCGACTATCACGCGACCCAGGCCG CGACCGTGGTCTCTGACCTGGACGGCGGCATCCGCGCCATGGTCGGCGGCCGCGACTACGGCGCCAGCCAGTTCAACCGC GCCACCGACGCGTACCGGCAGCCCGGCTCGTCGTTCAAGCCGTACGTGTACACGACCGCGCTCCTCAACGGCTTCAAGCC AACCTCGATCGTGGTCGACGGTCCGGTCTGCATCGGCAATTGGTGCCCGCAGAACTATGGCCACTCCTATTCCGGCTCGG TGACGCTGACCCAGGCGATCACGCGCTCGATCAATGTCGTGCCGGTCAAGCTGTCGATCGCGCTCGGCGGCAAGGACGGC CCGAAGGCGGGCCGCGCCAAGATCGTTGAGGTCGCCCGCCGCTTCGGCATCAAGGCGCCGCTGCCCGACACGCCGTCGAT GCCGATCGGCTCAGACGAGGTCACCGTGATCGAGCATGCGGTGGCGTATGCGACGTTCCCGAACAAGGGCAAGGCGGTGA CGCCGCACTCCGTGCTCGAGGTGCGCACCGGCGCCGGCGATCTGGTGTGGCGCTGGGACCGCGACGGGCCGAAGCCGAAA CAGGCGATCCCCGCCTCCGTCGCCGCCGACATGGCGGGCATGATGAGCCACGTCGTCAGCGAAGGCACCGCGCGCCGCGC CGCGCTCGACGGCATTCCGACCGCCGGCAAAACCGGCACCACCAACGCCTATCGCGACGCCTGGTTCGTCGGCTACACCG GCAACTTCACCTGCGCGGTGTGGTACGGCAACGACGACTACTCGCCGACCAACCGCATGACCGGCGGCTCGCTGCCGGCG CAGACCTGGCACGACATCATGGTCGCCGCGCATCAGGGCGTCGAGGTCAAGGAGTTGCCCGGCGTCGGCATGGGCGAGAA GCTGCCGCAGCCGGTGTCCGCCGCGATGGCCCAGGCCGGCCAGCCGAAGACACTCGAGATCAAGCCGGGCCCGCCGCCGA TTCTGACGCGCCGTGGCGCCGAGATCCTGGTGCAGGTCGAGAAGATGCTGGACGACGCCGCCCGCGTCGCCGAGAAGGCC GCGCCGGCCGATCCGAAGAAGCCCGGCAAGCCGGTGTCGTCGAGCGCGCTCGCCTTCCCCGAGAACTACGCCACGGCGAC CGCCGACAGCGTAACCACCCCTGCCTTGCGCAAGAACTGA
Upstream 100 bases:
>100_bases TTGCCGCAACAAGCGCGCATGACCCGTCGATGGCCCCCAGCCTCGCGTGCTGAGTGCTTTCGAAGCCCGCTGACACGCTT TCGAAACGACCAGGGTTCCC
Downstream 100 bases:
>100_bases CGACAGCGAAGTCCCGTGCGGCTGCTCTTCATCACCTTGCTCGCGCTGCTGATCGCCACCGGCGTCGGCATCGGCGCGAC CTGGATCACCACCACCCGCG
Product: putative penicillin-binding protein (mrcA-like)
Products: NA
Alternate protein names: PBP-1b; PBP1b; Murein polymerase; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Peptidoglycan glycosyltransferase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]
Number of amino acids: Translated: 759; Mature: 759
Protein sequence:
>759_residues MRQIIPDDWKKRINNFRLDLDARFDSALFSSLRGTRELYERFSAFMDRFYVGGWKRWVFVEPLSEAATIGLGGLVLMLAL AIPAFRETADEDWLKKSDLAVTFLDRYGNPIGSRGIKHNDSIPLEDFPDNLIKATLATEDRRFYDHFGIDFPGLARALVT NAQAGGVRQGGSSISQQLAKNLFLSNERTIERKVNEAFLAIWLETRLTKNEILKLYLDRAYMGGGTFGVDGAAHFYFNKS VRDINLAEAAMLAGLFKAPTKFAPHINLPAARARANVVLDNLVDAGFMTEGQVFGARRNPAFAVDRRDESSPNYFLDYAF DEMRKLVDTFPKSYTERVFVVRTSIDMNVQHAADEAIENQLRQFGRDYHATQAATVVSDLDGGIRAMVGGRDYGASQFNR ATDAYRQPGSSFKPYVYTTALLNGFKPTSIVVDGPVCIGNWCPQNYGHSYSGSVTLTQAITRSINVVPVKLSIALGGKDG PKAGRAKIVEVARRFGIKAPLPDTPSMPIGSDEVTVIEHAVAYATFPNKGKAVTPHSVLEVRTGAGDLVWRWDRDGPKPK QAIPASVAADMAGMMSHVVSEGTARRAALDGIPTAGKTGTTNAYRDAWFVGYTGNFTCAVWYGNDDYSPTNRMTGGSLPA QTWHDIMVAAHQGVEVKELPGVGMGEKLPQPVSAAMAQAGQPKTLEIKPGPPPILTRRGAEILVQVEKMLDDAARVAEKA APADPKKPGKPVSSSALAFPENYATATADSVTTPALRKN
Sequences:
>Translated_759_residues MRQIIPDDWKKRINNFRLDLDARFDSALFSSLRGTRELYERFSAFMDRFYVGGWKRWVFVEPLSEAATIGLGGLVLMLAL AIPAFRETADEDWLKKSDLAVTFLDRYGNPIGSRGIKHNDSIPLEDFPDNLIKATLATEDRRFYDHFGIDFPGLARALVT NAQAGGVRQGGSSISQQLAKNLFLSNERTIERKVNEAFLAIWLETRLTKNEILKLYLDRAYMGGGTFGVDGAAHFYFNKS VRDINLAEAAMLAGLFKAPTKFAPHINLPAARARANVVLDNLVDAGFMTEGQVFGARRNPAFAVDRRDESSPNYFLDYAF DEMRKLVDTFPKSYTERVFVVRTSIDMNVQHAADEAIENQLRQFGRDYHATQAATVVSDLDGGIRAMVGGRDYGASQFNR ATDAYRQPGSSFKPYVYTTALLNGFKPTSIVVDGPVCIGNWCPQNYGHSYSGSVTLTQAITRSINVVPVKLSIALGGKDG PKAGRAKIVEVARRFGIKAPLPDTPSMPIGSDEVTVIEHAVAYATFPNKGKAVTPHSVLEVRTGAGDLVWRWDRDGPKPK QAIPASVAADMAGMMSHVVSEGTARRAALDGIPTAGKTGTTNAYRDAWFVGYTGNFTCAVWYGNDDYSPTNRMTGGSLPA QTWHDIMVAAHQGVEVKELPGVGMGEKLPQPVSAAMAQAGQPKTLEIKPGPPPILTRRGAEILVQVEKMLDDAARVAEKA APADPKKPGKPVSSSALAFPENYATATADSVTTPALRKN >Mature_759_residues MRQIIPDDWKKRINNFRLDLDARFDSALFSSLRGTRELYERFSAFMDRFYVGGWKRWVFVEPLSEAATIGLGGLVLMLAL AIPAFRETADEDWLKKSDLAVTFLDRYGNPIGSRGIKHNDSIPLEDFPDNLIKATLATEDRRFYDHFGIDFPGLARALVT NAQAGGVRQGGSSISQQLAKNLFLSNERTIERKVNEAFLAIWLETRLTKNEILKLYLDRAYMGGGTFGVDGAAHFYFNKS VRDINLAEAAMLAGLFKAPTKFAPHINLPAARARANVVLDNLVDAGFMTEGQVFGARRNPAFAVDRRDESSPNYFLDYAF DEMRKLVDTFPKSYTERVFVVRTSIDMNVQHAADEAIENQLRQFGRDYHATQAATVVSDLDGGIRAMVGGRDYGASQFNR ATDAYRQPGSSFKPYVYTTALLNGFKPTSIVVDGPVCIGNWCPQNYGHSYSGSVTLTQAITRSINVVPVKLSIALGGKDG PKAGRAKIVEVARRFGIKAPLPDTPSMPIGSDEVTVIEHAVAYATFPNKGKAVTPHSVLEVRTGAGDLVWRWDRDGPKPK QAIPASVAADMAGMMSHVVSEGTARRAALDGIPTAGKTGTTNAYRDAWFVGYTGNFTCAVWYGNDDYSPTNRMTGGSLPA QTWHDIMVAAHQGVEVKELPGVGMGEKLPQPVSAAMAQAGQPKTLEIKPGPPPILTRRGAEILVQVEKMLDDAARVAEKA APADPKKPGKPVSSSALAFPENYATATADSVTTPALRKN
Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal
COG id: COG0744
COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)
Gene ontology:
Cell location: Cell inner membrane [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: In the C-terminal section; belongs to the transpeptidase family [H]
Homologues:
Organism=Escherichia coli, GI1786343, Length=536, Percent_Identity=33.3955223880597, Blast_Score=230, Evalue=3e-61, Organism=Escherichia coli, GI87082258, Length=259, Percent_Identity=38.996138996139, Blast_Score=177, Evalue=2e-45, Organism=Escherichia coli, GI1788867, Length=517, Percent_Identity=29.2069632495164, Blast_Score=176, Evalue=4e-45, Organism=Escherichia coli, GI1789601, Length=183, Percent_Identity=34.9726775956284, Blast_Score=107, Evalue=2e-24,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR012338 - InterPro: IPR001264 - InterPro: IPR011813 - InterPro: IPR001460 [H]
Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]
EC number: =2.4.1.129 [H]
Molecular weight: Translated: 82872; Mature: 82872
Theoretical pI: Translated: 9.10; Mature: 9.10
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.4 %Cys (Translated Protein) 2.4 %Met (Translated Protein) 2.8 %Cys+Met (Translated Protein) 0.4 %Cys (Mature Protein) 2.4 %Met (Mature Protein) 2.8 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MRQIIPDDWKKRINNFRLDLDARFDSALFSSLRGTRELYERFSAFMDRFYVGGWKRWVFV CCCCCCHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEE EPLSEAATIGLGGLVLMLALAIPAFRETADEDWLKKSDLAVTFLDRYGNPIGSRGIKHND CCHHHHHHHCHHHHHHHHHHHHHHHHHCCCHHHHHHCCCEEEEHHHHCCCCCCCCCCCCC SIPLEDFPDNLIKATLATEDRRFYDHFGIDFPGLARALVTNAQAGGVRQGGSSISQQLAK CCCCCCCCHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHH NLFLSNERTIERKVNEAFLAIWLETRLTKNEILKLYLDRAYMGGGTFGVDGAAHFYFNKS HHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCC VRDINLAEAAMLAGLFKAPTKFAPHINLPAARARANVVLDNLVDAGFMTEGQVFGARRNP CCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEECCCCCC AFAVDRRDESSPNYFLDYAFDEMRKLVDTFPKSYTERVFVVRTSIDMNVQHAADEAIENQ CEEECCCCCCCCCEEEEHHHHHHHHHHHHHCHHHCCEEEEEEECCCCCHHHHHHHHHHHH LRQFGRDYHATQAATVVSDLDGGIRAMVGGRDYGASQFNRATDAYRQPGSSFKPYVYTTA HHHHCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCEEEHH LLNGFKPTSIVVDGPVCIGNWCPQNYGHSYSGSVTLTQAITRSINVVPVKLSIALGGKDG HHCCCCCCEEEEECCEEECCCCCCCCCCCCCCCEEHHHHHHCCCEEEEEEEEEEECCCCC PKAGRAKIVEVARRFGIKAPLPDTPSMPIGSDEVTVIEHAVAYATFPNKGKAVTPHSVLE CCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHEECCCCCCEECCHHHEE VRTGAGDLVWRWDRDGPKPKQAIPASVAADMAGMMSHVVSEGTARRAALDGIPTAGKTGT EECCCCCEEEEECCCCCCCHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCC TNAYRDAWFVGYTGNFTCAVWYGNDDYSPTNRMTGGSLPAQTWHDIMVAAHQGVEVKELP CCCCCCEEEEEECCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHCC GVGMGEKLPQPVSAAMAQAGQPKTLEIKPGPPPILTRRGAEILVQVEKMLDDAARVAEKA CCCCCCCCCCHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC APADPKKPGKPVSSSALAFPENYATATADSVTTPALRKN CCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCHHCCC >Mature Secondary Structure MRQIIPDDWKKRINNFRLDLDARFDSALFSSLRGTRELYERFSAFMDRFYVGGWKRWVFV CCCCCCHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEE EPLSEAATIGLGGLVLMLALAIPAFRETADEDWLKKSDLAVTFLDRYGNPIGSRGIKHND CCHHHHHHHCHHHHHHHHHHHHHHHHHCCCHHHHHHCCCEEEEHHHHCCCCCCCCCCCCC SIPLEDFPDNLIKATLATEDRRFYDHFGIDFPGLARALVTNAQAGGVRQGGSSISQQLAK CCCCCCCCHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHH NLFLSNERTIERKVNEAFLAIWLETRLTKNEILKLYLDRAYMGGGTFGVDGAAHFYFNKS HHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCC VRDINLAEAAMLAGLFKAPTKFAPHINLPAARARANVVLDNLVDAGFMTEGQVFGARRNP CCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEECCCCCC AFAVDRRDESSPNYFLDYAFDEMRKLVDTFPKSYTERVFVVRTSIDMNVQHAADEAIENQ CEEECCCCCCCCCEEEEHHHHHHHHHHHHHCHHHCCEEEEEEECCCCCHHHHHHHHHHHH LRQFGRDYHATQAATVVSDLDGGIRAMVGGRDYGASQFNRATDAYRQPGSSFKPYVYTTA HHHHCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCEEEHH LLNGFKPTSIVVDGPVCIGNWCPQNYGHSYSGSVTLTQAITRSINVVPVKLSIALGGKDG HHCCCCCCEEEEECCEEECCCCCCCCCCCCCCCEEHHHHHHCCCEEEEEEEEEEECCCCC PKAGRAKIVEVARRFGIKAPLPDTPSMPIGSDEVTVIEHAVAYATFPNKGKAVTPHSVLE CCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHEECCCCCCEECCHHHEE VRTGAGDLVWRWDRDGPKPKQAIPASVAADMAGMMSHVVSEGTARRAALDGIPTAGKTGT EECCCCCEEEEECCCCCCCHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCC TNAYRDAWFVGYTGNFTCAVWYGNDDYSPTNRMTGGSLPAQTWHDIMVAAHQGVEVKELP CCCCCCEEEEEECCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHCC GVGMGEKLPQPVSAAMAQAGQPKTLEIKPGPPPILTRRGAEILVQVEKMLDDAARVAEKA CCCCCCCCCCHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC APADPKKPGKPVSSSALAFPENYATATADSVTTPALRKN CCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCHHCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: 7542800 [H]