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The map label for this gene is pleD [H]

Identifier: 146339674

GI number: 146339674

Start: 2870732

End: 2871880

Strand: Reverse

Name: pleD [H]

Synonym: BRADO2669

Alternate gene names: 146339674

Gene position: 2871880-2870732 (Counterclockwise)

Preceding gene: 146339677

Following gene: 146339673

Centisome position: 38.51

GC content: 66.84

Gene sequence:

>1149_bases
ATGGACACTACGGCGCCGCCCTATCGAAATGCCGATGTTGCCGAGGTGGACGGCTCGCGCGCGCTGCCGCCGCCCGATCT
GCATGTCCGCCGCGCCGGACGGCGCCGGCAGATGCTGGCGATGATCGCCGGCTGCTACCTGATCGACGCGGTGATCCTGT
TCATCTATGCCCAGGCCGGTACCGTACCGCCCACGATCGCGCCCTCTTATGCCGCGATTGGCGTGCTGACCGTCGCTCTG
TGGACGATGCTGTCGGAGAGCGGGTTCAACGACCGCTTCCGCGACCACTATCTGGTCGTGCCGCAGTCGATCGCCAGCAT
GATGATGACGGTCGGTTTCTGCTACATTGCGCCGGAAGTCAGCATCGTCTTCCTGTGCACGCTGTATCTGGTCGTCGCCT
TCGGCTCGCTCCGCGCCACGCCACGCCAGAGCGCGATCTGCTGGACCTTCCTGGCGCTCGGCCTCGCCGGCCTATTCCTC
CTGACGGACAAGCCACTGGGCATGCCAACCGGCAACGCCCTGGAGCGGCTCGCGACCCTCCTGGTGTTCCTGCTCACCAT
CGCCGGCTGCATGTTCCTCGGCATCTTCTCCTCGGGCCTGCGCGAGACGCTGTATCTACGCGGTCAGAAGCTGAAGGAGG
CCTATCGCCGCATCGAGGAGCTCGCCGAGCTCGACGAGCTGACCGGCGCGCTGAACCGCCGTTCGATCATGCAGGTGCTC
GATGAGGAAATGGCGCGCTGCCGCCAGGCCGGCCGGCCCTGCTCGATCGTGCTGATCGACCTCGACTGGTTCAAGCGCAT
CAACGACACCCATGGCCATCCAACCGGCGACGACGTGCTGCGCACCTTCGCGATCACGATGTACGCCAACATTCGCGGCA
GTGACCGTTTTGGACGCTATGGCGGAGAGGAATTCCTGCTGGTGCTCCCGGGCGCGCCGGCGGAGCCCGCCGCACGGCTC
GCCGAACGGCTGCGCCGGATTATTGCCGAGCTCGACTGGAGCGCGTTCTCCGCCGGCCTGCAGGTCACGTTCTCGGCGGG
CGTCGCGACGCGGCGCGGCGACGAGACCGCCGAGTCCCTGCTCGCCCGCGCGGATCATGCGCTCTACGACTCCAAGGCCA
ATGGGCGCAATCGCGTCACCGGCACCTGA

Upstream 100 bases:

>100_bases
TCACGACGCGCGGCCTCGCGCGCATTCCAGTTCCCTAGATACCAAAGTTAAAGGCTTTCCCGCGTAGATTTTCGGTTGAA
CTCCGAAGAAATGCGAGCAA

Downstream 100 bases:

>100_bases
GCATCCCAGCATGCCCGCGAAGTCCGCGCCGAACGAGAGCCTGCTGGACGAGCTGGAGGACACGCTCGCGCACGGCACCG
TGGCGCGACGGGTCGAGACG

Product: hypothetical protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MDTTAPPYRNADVAEVDGSRALPPPDLHVRRAGRRRQMLAMIAGCYLIDAVILFIYAQAGTVPPTIAPSYAAIGVLTVAL
WTMLSESGFNDRFRDHYLVVPQSIASMMMTVGFCYIAPEVSIVFLCTLYLVVAFGSLRATPRQSAICWTFLALGLAGLFL
LTDKPLGMPTGNALERLATLLVFLLTIAGCMFLGIFSSGLRETLYLRGQKLKEAYRRIEELAELDELTGALNRRSIMQVL
DEEMARCRQAGRPCSIVLIDLDWFKRINDTHGHPTGDDVLRTFAITMYANIRGSDRFGRYGGEEFLLVLPGAPAEPAARL
AERLRRIIAELDWSAFSAGLQVTFSAGVATRRGDETAESLLARADHALYDSKANGRNRVTGT

Sequences:

>Translated_382_residues
MDTTAPPYRNADVAEVDGSRALPPPDLHVRRAGRRRQMLAMIAGCYLIDAVILFIYAQAGTVPPTIAPSYAAIGVLTVAL
WTMLSESGFNDRFRDHYLVVPQSIASMMMTVGFCYIAPEVSIVFLCTLYLVVAFGSLRATPRQSAICWTFLALGLAGLFL
LTDKPLGMPTGNALERLATLLVFLLTIAGCMFLGIFSSGLRETLYLRGQKLKEAYRRIEELAELDELTGALNRRSIMQVL
DEEMARCRQAGRPCSIVLIDLDWFKRINDTHGHPTGDDVLRTFAITMYANIRGSDRFGRYGGEEFLLVLPGAPAEPAARL
AERLRRIIAELDWSAFSAGLQVTFSAGVATRRGDETAESLLARADHALYDSKANGRNRVTGT
>Mature_382_residues
MDTTAPPYRNADVAEVDGSRALPPPDLHVRRAGRRRQMLAMIAGCYLIDAVILFIYAQAGTVPPTIAPSYAAIGVLTVAL
WTMLSESGFNDRFRDHYLVVPQSIASMMMTVGFCYIAPEVSIVFLCTLYLVVAFGSLRATPRQSAICWTFLALGLAGLFL
LTDKPLGMPTGNALERLATLLVFLLTIAGCMFLGIFSSGLRETLYLRGQKLKEAYRRIEELAELDELTGALNRRSIMQVL
DEEMARCRQAGRPCSIVLIDLDWFKRINDTHGHPTGDDVLRTFAITMYANIRGSDRFGRYGGEEFLLVLPGAPAEPAARL
AERLRRIIAELDWSAFSAGLQVTFSAGVATRRGDETAESLLARADHALYDSKANGRNRVTGT

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=209, Percent_Identity=35.8851674641148, Blast_Score=121, Evalue=7e-29,
Organism=Escherichia coli, GI87081881, Length=168, Percent_Identity=39.2857142857143, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1786584, Length=177, Percent_Identity=36.7231638418079, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1787262, Length=170, Percent_Identity=35.2941176470588, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI87082007, Length=201, Percent_Identity=34.3283582089552, Blast_Score=98, Evalue=8e-22,
Organism=Escherichia coli, GI1787816, Length=159, Percent_Identity=35.2201257861635, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI1788381, Length=170, Percent_Identity=33.5294117647059, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1787802, Length=252, Percent_Identity=25.7936507936508, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87081974, Length=154, Percent_Identity=29.8701298701299, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787056, Length=231, Percent_Identity=30.7359307359307, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1788085, Length=166, Percent_Identity=32.5301204819277, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787541, Length=172, Percent_Identity=29.0697674418605, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 42019; Mature: 42019

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure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HHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA