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The map label for this gene is yafV [C]

Identifier: 146339663

GI number: 146339663

Start: 2858554

End: 2859384

Strand: Reverse

Name: yafV [C]

Synonym: BRADO2656

Alternate gene names: 146339663

Gene position: 2859384-2858554 (Counterclockwise)

Preceding gene: 146339664

Following gene: 146339662

Centisome position: 38.35

GC content: 66.67

Gene sequence:

>831_bases
ATGCAGCCGATGGTCGGCGAGTTGGCCACCAACCTCGCCCACAGCCTCGATTTGATCCAACAGGCGGTGGCGCAAGGCGC
CAAGCTCGTGGTGCTCCCCGAACTCGCCTCCTCCGGCTACGTGTTCAAATCCCGCGAGGAGGCCTTCGCCGCCTCGGAGA
CAATTCCCAACGGCCCCGCGATGACCGCATGGAGCGAGATCGCGGCCAAGCACGGCCTGCATCTCGTCGCCGGCATATGC
GAGCGCGACGGGACCAAGCTCTACAACAGCGCGGTGCTGATCGGGCCGAAGGGCTACATCGGCACGTTCCGCAAGGTGCA
CCTGTGGAACGAGGAGAACCTGTATTTCGAGCCCGGCAATCTGGGCTTCCCCGTCTACCACACCGAGATCGGCCGCATCG
GCATGGCGATCTGCTATGACGGGTGGTTCCCGGAGACCTATCGCCTCAACGCGCTGCAGGGCGCCGACATCGTCTGCGTG
CCGACGAACTGGGTGCCGATCCCTGGCCAGGCGCCGGGACGCGAGGCCATGGCCAACATCCTGGCGATGGCGGCGGCGCA
TTCCAACTCCATCTTCATCGCCTGCGCCGACCGCGTCGGCGTCGAGCGCGGTCAGCCGTTCGAAGGCCAGAGCCTGATCG
TCAGCTACACCGGCTGGCCGGTCGCCGGTCCTGCGAGCCGCGACAAGGAGGAGATCGTGATCGCCGATGTGGCGCTCGGC
GAGGCCCGCCGCGCCCGCAACTGGAACGCCTTCAACCAGGTCCTGCGCGACCGTCGCTCCGACGTCTACGACGAGATGCT
CGGCAGCGAGGTCAAGCGCGGTTGGTACTGA

Upstream 100 bases:

>100_bases
GGGGGCTTTTTGTTTGGCGCGCGCGATCCTCGCGAACGCAACGGAGACTCGTGCCTTGACGACGGATGACGACAGCAAAC
TGCGGATCGCCTGCATCCAG

Downstream 100 bases:

>100_bases
CAACTTCGCCTTTCTGTTTTGACCATTCATGGAGGACCAACGATGACATCACGTTTGCTCACCACTTCACTCGCGCTCGC
CTTCGCGGCGACCGCGCTCT

Product: putative N-carbamoyl-D-amino acid hydrolase

Products: NA

Alternate protein names: D-N-alpha-carbamilase [H]

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MQPMVGELATNLAHSLDLIQQAVAQGAKLVVLPELASSGYVFKSREEAFAASETIPNGPAMTAWSEIAAKHGLHLVAGIC
ERDGTKLYNSAVLIGPKGYIGTFRKVHLWNEENLYFEPGNLGFPVYHTEIGRIGMAICYDGWFPETYRLNALQGADIVCV
PTNWVPIPGQAPGREAMANILAMAAAHSNSIFIACADRVGVERGQPFEGQSLIVSYTGWPVAGPASRDKEEIVIADVALG
EARRARNWNAFNQVLRDRRSDVYDEMLGSEVKRGWY

Sequences:

>Translated_276_residues
MQPMVGELATNLAHSLDLIQQAVAQGAKLVVLPELASSGYVFKSREEAFAASETIPNGPAMTAWSEIAAKHGLHLVAGIC
ERDGTKLYNSAVLIGPKGYIGTFRKVHLWNEENLYFEPGNLGFPVYHTEIGRIGMAICYDGWFPETYRLNALQGADIVCV
PTNWVPIPGQAPGREAMANILAMAAAHSNSIFIACADRVGVERGQPFEGQSLIVSYTGWPVAGPASRDKEEIVIADVALG
EARRARNWNAFNQVLRDRRSDVYDEMLGSEVKRGWY
>Mature_276_residues
MQPMVGELATNLAHSLDLIQQAVAQGAKLVVLPELASSGYVFKSREEAFAASETIPNGPAMTAWSEIAAKHGLHLVAGIC
ERDGTKLYNSAVLIGPKGYIGTFRKVHLWNEENLYFEPGNLGFPVYHTEIGRIGMAICYDGWFPETYRLNALQGADIVCV
PTNWVPIPGQAPGREAMANILAMAAAHSNSIFIACADRVGVERGQPFEGQSLIVSYTGWPVAGPASRDKEEIVIADVALG
EARRARNWNAFNQVLRDRRSDVYDEMLGSEVKRGWY

Specific function: The enzyme catalyzes the hydrolysis of N-carbamoyl-D- amino acids to the corresponding which are useful intermediates in the preparation of beta-lactam antibiotics. Industrial production of beta-lactam antibiotics is now being developed using this enzyme

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=267, Percent_Identity=29.2134831460674, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI7706509, Length=196, Percent_Identity=30.6122448979592, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI297632348, Length=274, Percent_Identity=27.7372262773723, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI5031947, Length=274, Percent_Identity=27.7372262773723, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI297632350, Length=274, Percent_Identity=27.7372262773723, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI297632346, Length=178, Percent_Identity=31.4606741573034, Blast_Score=65, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17533173, Length=280, Percent_Identity=31.7857142857143, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17557111, Length=148, Percent_Identity=29.0540540540541, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6323383, Length=275, Percent_Identity=28, Blast_Score=73, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6322027, Length=178, Percent_Identity=32.0224719101124, Blast_Score=67, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21358471, Length=270, Percent_Identity=29.6296296296296, Blast_Score=97, Evalue=9e-21,
Organism=Drosophila melanogaster, GI17933642, Length=269, Percent_Identity=28.996282527881, Blast_Score=77, Evalue=9e-15,
Organism=Drosophila melanogaster, GI21355835, Length=267, Percent_Identity=26.2172284644195, Blast_Score=75, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.77 [H]

Molecular weight: Translated: 30194; Mature: 30194

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPMVGELATNLAHSLDLIQQAVAQGAKLVVLPELASSGYVFKSREEAFAASETIPNGPA
CCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEECHHHHHHHHCCCCCCCC
MTAWSEIAAKHGLHLVAGICERDGTKLYNSAVLIGPKGYIGTFRKVHLWNEENLYFEPGN
HHHHHHHHHHCCHHEEHHHHHCCCCEEECCEEEECCCCCCCCEEEEEEECCCCEEECCCC
LGFPVYHTEIGRIGMAICYDGWFPETYRLNALQGADIVCVPTNWVPIPGQAPGREAMANI
CCCCEEECHHHHEEEEEEECCCCCCCEEEEEECCCCEEEECCCCEECCCCCCCHHHHHHH
LAMAAAHSNSIFIACADRVGVERGQPFEGQSLIVSYTGWPVAGPASRDKEEIVIADVALG
HHHHHHCCCCEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEEEEECC
EARRARNWNAFNQVLRDRRSDVYDEMLGSEVKRGWY
CHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQPMVGELATNLAHSLDLIQQAVAQGAKLVVLPELASSGYVFKSREEAFAASETIPNGPA
CCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEECHHHHHHHHCCCCCCCC
MTAWSEIAAKHGLHLVAGICERDGTKLYNSAVLIGPKGYIGTFRKVHLWNEENLYFEPGN
HHHHHHHHHHCCHHEEHHHHHCCCCEEECCEEEECCCCCCCCEEEEEEECCCCEEECCCC
LGFPVYHTEIGRIGMAICYDGWFPETYRLNALQGADIVCVPTNWVPIPGQAPGREAMANI
CCCCEEECHHHHEEEEEEECCCCCCCEEEEEECCCCEEEECCCCEECCCCCCCHHHHHHH
LAMAAAHSNSIFIACADRVGVERGQPFEGQSLIVSYTGWPVAGPASRDKEEIVIADVALG
HHHHHHCCCCEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEEEEECC
EARRARNWNAFNQVLRDRRSDVYDEMLGSEVKRGWY
CHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9648217; 10903946 [H]