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The map label for this gene is amiC [H]

Identifier: 146339662

GI number: 146339662

Start: 2857351

End: 2858511

Strand: Reverse

Name: amiC [H]

Synonym: BRADO2655

Alternate gene names: 146339662

Gene position: 2858511-2857351 (Counterclockwise)

Preceding gene: 146339663

Following gene: 146339661

Centisome position: 38.34

GC content: 64.34

Gene sequence:

>1161_bases
ATGACATCACGTTTGCTCACCACTTCACTCGCGCTCGCCTTCGCGGCGACCGCGCTCTCGTCCGCTTTCGCCGCCGATCC
GATCAAGATCGGCGTTCCCGTCGGCCTGTCCGGCGCCAACTCCGTCGTCGCGCCGTCGGTGGTGCAATCCGCCGAGCTGG
CGGTCGAGGAGATCAACGCCAAGGGCGGCGTGCTCGGCCGCAAGCTGGAGCTGGAAGTCGCTGACGACGCTTCTGGCGCT
GCCGGCGCGCAGAAGGCGTTCGACGCGCTGATCTTCCAGAAGAAGGTCAACGTGCTGATCTCGATGGAGACCAGCGCGGC
CCGCAATGCCGGCCTGCCGATCGTCGCGCGCGGCAAGACGCCCTACATCTACACCTCGTTCTACGAGGGTAAGTCGTGCA
GCCCCTATCTCTACGTCAACGCCTGGGTGCCGGACCAGCAGGTGCCGCCGATCGTCGACTACTTCAACAAGGAGAAGAAG
GCGAAGAACTACTTCCTGATCGGCTCCGATTATGCCTTCGGCCGCGGCATGCTCGCGTTTACGAAGATGTATATCGAGAA
GACCGGCGGCAAAGTCGTCGGCGAGGAATATCTGCCGATGGACGGCTCCGACTGGACCTCGATCATCTCCAAGCTGAAGG
CCTCTGGCGCCGATGCGCTGATCACCTCGACCGCGGGCGGCGCGCCGAACGTCACCCTCACCAAGCAGCTGCGCGCCGCC
GGCGTGAACCTGCCCTACGGCAATCTCGCCGTGGACGAAGGCACAGCCAAGGGCATGGGCGCCGATGCTGCCGGCATCTA
TCTGTCTGCCTCCTACGTGACCGGCATCGACACGCCGGCCAACAAGACCTTCCTGGCCTCGATGGCGAAGAAGTTCGGCG
CCGAGCTCAAGACCCCGAATGATCTCTCGGTTCCGCAGTACGAGGCCGTCTACGCCTACAAGGCCGCGGTCGAGAAGGCC
GGCAGCACCGATCAGGACAAAGTACTGAAGGCGCTCGCCGAGGTCTCGGTCGATGGTCCCCGCGGCACCATCAAGATGGA
CAAGCAGCGCCACGCGCCGCTGACCATGTATCTCGGCCAGGTTCAGGCCGACGGCAGCGTCAAGGTGATCCAGAGCTTCA
AGGACGTCGATCCCGGCGCGCAGTGCCCCGGCAAGTCGTGA

Upstream 100 bases:

>100_bases
TCGCTCCGACGTCTACGACGAGATGCTCGGCAGCGAGGTCAAGCGCGGTTGGTACTGACAACTTCGCCTTTCTGTTTTGA
CCATTCATGGAGGACCAACG

Downstream 100 bases:

>100_bases
AACAGCACGCCTGATCGCGTATGATCCCGCGGGGCGGACTACAGCCCGCCCCGCTTCTCTCAAGTGGAAGTGACCATGCT
GACCGTCGCCCTCGATGCCG

Product: putative signal peptide

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MTSRLLTTSLALAFAATALSSAFAADPIKIGVPVGLSGANSVVAPSVVQSAELAVEEINAKGGVLGRKLELEVADDASGA
AGAQKAFDALIFQKKVNVLISMETSAARNAGLPIVARGKTPYIYTSFYEGKSCSPYLYVNAWVPDQQVPPIVDYFNKEKK
AKNYFLIGSDYAFGRGMLAFTKMYIEKTGGKVVGEEYLPMDGSDWTSIISKLKASGADALITSTAGGAPNVTLTKQLRAA
GVNLPYGNLAVDEGTAKGMGADAAGIYLSASYVTGIDTPANKTFLASMAKKFGAELKTPNDLSVPQYEAVYAYKAAVEKA
GSTDQDKVLKALAEVSVDGPRGTIKMDKQRHAPLTMYLGQVQADGSVKVIQSFKDVDPGAQCPGKS

Sequences:

>Translated_386_residues
MTSRLLTTSLALAFAATALSSAFAADPIKIGVPVGLSGANSVVAPSVVQSAELAVEEINAKGGVLGRKLELEVADDASGA
AGAQKAFDALIFQKKVNVLISMETSAARNAGLPIVARGKTPYIYTSFYEGKSCSPYLYVNAWVPDQQVPPIVDYFNKEKK
AKNYFLIGSDYAFGRGMLAFTKMYIEKTGGKVVGEEYLPMDGSDWTSIISKLKASGADALITSTAGGAPNVTLTKQLRAA
GVNLPYGNLAVDEGTAKGMGADAAGIYLSASYVTGIDTPANKTFLASMAKKFGAELKTPNDLSVPQYEAVYAYKAAVEKA
GSTDQDKVLKALAEVSVDGPRGTIKMDKQRHAPLTMYLGQVQADGSVKVIQSFKDVDPGAQCPGKS
>Mature_385_residues
TSRLLTTSLALAFAATALSSAFAADPIKIGVPVGLSGANSVVAPSVVQSAELAVEEINAKGGVLGRKLELEVADDASGAA
GAQKAFDALIFQKKVNVLISMETSAARNAGLPIVARGKTPYIYTSFYEGKSCSPYLYVNAWVPDQQVPPIVDYFNKEKKA
KNYFLIGSDYAFGRGMLAFTKMYIEKTGGKVVGEEYLPMDGSDWTSIISKLKASGADALITSTAGGAPNVTLTKQLRAAG
VNLPYGNLAVDEGTAKGMGADAAGIYLSASYVTGIDTPANKTFLASMAKKFGAELKTPNDLSVPQYEAVYAYKAAVEKAG
STDQDKVLKALAEVSVDGPRGTIKMDKQRHAPLTMYLGQVQADGSVKVIQSFKDVDPGAQCPGKS

Specific function: Negatively regulates the expression of the aliphatic amidase operon. AmiC functions by inhibiting the action of AmiR at the protein level. It exhibits protein kinase activity [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000709 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 40486; Mature: 40354

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSRLLTTSLALAFAATALSSAFAADPIKIGVPVGLSGANSVVAPSVVQSAELAVEEINA
CCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCC
KGGVLGRKLELEVADDASGAAGAQKAFDALIFQKKVNVLISMETSAARNAGLPIVARGKT
CCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCEEEEEECCHHHHCCCCEEEECCCC
PYIYTSFYEGKSCSPYLYVNAWVPDQQVPPIVDYFNKEKKAKNYFLIGSDYAFGRGMLAF
CEEEEEEECCCCCCCEEEEEEECCCCCCCHHHHHHCCHHCCCCEEEEECCCCCCCHHHHH
TKMYIEKTGGKVVGEEYLPMDGSDWTSIISKLKASGADALITSTAGGAPNVTLTKQLRAA
HHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCEEHHHHHHC
GVNLPYGNLAVDEGTAKGMGADAAGIYLSASYVTGIDTPANKTFLASMAKKFGAELKTPN
CCCCCCCCEEECCCCCCCCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCC
DLSVPQYEAVYAYKAAVEKAGSTDQDKVLKALAEVSVDGPRGTIKMDKQRHAPLTMYLGQ
CCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCCCCEEEEEEE
VQADGSVKVIQSFKDVDPGAQCPGKS
EECCCCHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TSRLLTTSLALAFAATALSSAFAADPIKIGVPVGLSGANSVVAPSVVQSAELAVEEINA
CCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCC
KGGVLGRKLELEVADDASGAAGAQKAFDALIFQKKVNVLISMETSAARNAGLPIVARGKT
CCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCEEEEEECCHHHHCCCCEEEECCCC
PYIYTSFYEGKSCSPYLYVNAWVPDQQVPPIVDYFNKEKKAKNYFLIGSDYAFGRGMLAF
CEEEEEEECCCCCCCEEEEEEECCCCCCCHHHHHHCCHHCCCCEEEEECCCCCCCHHHHH
TKMYIEKTGGKVVGEEYLPMDGSDWTSIISKLKASGADALITSTAGGAPNVTLTKQLRAA
HHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCEEHHHHHHC
GVNLPYGNLAVDEGTAKGMGADAAGIYLSASYVTGIDTPANKTFLASMAKKFGAELKTPN
CCCCCCCCEEECCCCCCCCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCC
DLSVPQYEAVYAYKAAVEKAGSTDQDKVLKALAEVSVDGPRGTIKMDKQRHAPLTMYLGQ
CCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCCCCEEEEEEE
VQADGSVKVIQSFKDVDPGAQCPGKS
EECCCCHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1907262; 10984043; 1762155; 7813419; 10508151; 10708652 [H]