Click here to switch to the map view.

The map label for this gene is alkJ [H]

Identifier: 146339648

GI number: 146339648

Start: 2835647

End: 2837266

Strand: Reverse

Name: alkJ [H]

Synonym: BRADO2641

Alternate gene names: 146339648

Gene position: 2837266-2835647 (Counterclockwise)

Preceding gene: 146339650

Following gene: 146339646

Centisome position: 38.05

GC content: 67.35

Gene sequence:

>1620_bases
ATGCCGCGTCAGCTCGAGGGAGAATACGACTACATCGTGGTGGGCGCCGGCACTGCCGGCTGCATCCTGGCGAACCGGCT
GTCGGCCGATCCGAAGACCCGGGTGCTGATCCTCGAGGCCGGCGGCTACGACAATTGGATCTGGTTCCATATTCCGGTCG
GCTACCTCTTCGCGATCGGCAATCCGCGCTCCGACTGGATGTTTCGCACCGAATCCGAGCCCGGCCTCAACGGCCGCTCC
CTGGCCTATCCGCGCGGCAAGGTGATCGGCGGCTGCTCGGCGATCAATGCGATGATCTCGATGCGCGGCCAGGCCGCCGA
CTACGACCATTGGCGCCAGCTGGGCCTGCCCGGCTGGTCCTACTCTGACGTGCTGGCGGTGTTCCGGCGGCTGGAGGACC
ACTTTCTGGGCGAGAGCGAGCACCACGGCGTGGGCGGCGGCTGGCGGATCGAGGCGCCGCGGTTGTCCTGGGCGGTGCTG
GATGCCGTCGGCGATGCCGCCGAACAGATGGGCATCCGCCGCATCCCGGATTTCAATACCGGCGACAATGAAGGCATCAG
CTATTTCCACGTCAACCAGCGGCGCGGCCGGCGCTGGTCCTCGGCACGGGGTTTTCTCAAGCCGGCACTGAAGCGGCAGA
ACCTACGCCTCGAAACCAACGTCCTGGTCGATCGCCTGATCGTCGAGAACGGCCGCGTGACCGGCGTCCGCTTCCAGCAG
GACGGGCAGGCCATCGAGGCGCGAGCCAAGGGCGAGGTGATCCTGAGCGCCGGCTCGATCGGCTCGGTTCAGGTGCTGCA
GCGCTCGGGTATCGGGCCGGCCGAGTGGCTCGGCGAGCTCGGCATCGATGTCGTATTGGACAAGCCCGGCGTCGGGCGCA
ATCTGCAGGACCATCTGCAGCAGCGCGCGATCTACAAGGTTTCAGGCGTGAAGACGCTGAATGAGACCTACTACTCGCTG
GTCAAGCGCGGCCTGATGGGGCTCGACTACGCCTTCCGCCGCCGCGGGCCGCTGACCATGGCGCCATCCCAGCTCGGCAT
CTTCACGCGCTCCGATCCCCGGCGTGACCGCGCCAACATCCAGTTCCACGTGCAGCCGCTGTCGCTCGACAAGTTCGGCG
ATCCGCTGCACCGTTTCCCGGCAATCACGGTGAGCGCCTGCAACCTGCGCCCGACCTCGCGCGGCACGGTGCGCATCCGC
TCCGCAAAGATCGATGAGGCGCCGTCGATCGCGCCGAACTATCTCGCGACCGAGGACGACCGCGAGGTCGCCGCCGACGC
CATCCGCATCACCCGGCGGCTGATGAAGCAGAGCGCGCTGGCGGCCTACCGGCCGGAAGAATATTTGCCGGGCCCGTCCG
TCGGCGATGACGATGCGGCGCTGGCGAAGGCCGCCGGCGACATCGGCACCACGATCTTCCATCCCGTCGGCACGGCGAAG
ATGGGCACTGCTGACGATCCGATGGCGGTGGTCGACGAACGGCTGCGCTTCCGCGGCCTTGACGGCCTGCGCATCGCCGA
CGCCTCGATCATGCCGACGATCACGTCGGGAAATACCAACACGCCGACGGCGATGATCGCGGAGAAGGCGGCGGGGATGA
TCCTGGAGGACGGACGCTGA

Upstream 100 bases:

>100_bases
CGCCTGTTTCATGGCTCGGTCAACCAGCCATGAACGAGGCGCTTGTCCGGCCCCGCTCCGCCTGACTAAAAGCCTCGCAA
CGATTGGGAAGGGAATGCCG

Downstream 100 bases:

>100_bases
CGACCTCTATGCCGCTCGCCTGTAGCGAGCGGCATGACGCGAGATCGCACTCCTGACGCTGCACGCTATGGGCTGTACGC
TTGACGCCGCGCGAACGGGC

Product: GMC-type oxidoreductase

Products: Betaine Aldehyde; Reduced Acceptor. [C]

Alternate protein names: NA

Number of amino acids: Translated: 539; Mature: 538

Protein sequence:

>539_residues
MPRQLEGEYDYIVVGAGTAGCILANRLSADPKTRVLILEAGGYDNWIWFHIPVGYLFAIGNPRSDWMFRTESEPGLNGRS
LAYPRGKVIGGCSAINAMISMRGQAADYDHWRQLGLPGWSYSDVLAVFRRLEDHFLGESEHHGVGGGWRIEAPRLSWAVL
DAVGDAAEQMGIRRIPDFNTGDNEGISYFHVNQRRGRRWSSARGFLKPALKRQNLRLETNVLVDRLIVENGRVTGVRFQQ
DGQAIEARAKGEVILSAGSIGSVQVLQRSGIGPAEWLGELGIDVVLDKPGVGRNLQDHLQQRAIYKVSGVKTLNETYYSL
VKRGLMGLDYAFRRRGPLTMAPSQLGIFTRSDPRRDRANIQFHVQPLSLDKFGDPLHRFPAITVSACNLRPTSRGTVRIR
SAKIDEAPSIAPNYLATEDDREVAADAIRITRRLMKQSALAAYRPEEYLPGPSVGDDDAALAKAAGDIGTTIFHPVGTAK
MGTADDPMAVVDERLRFRGLDGLRIADASIMPTITSGNTNTPTAMIAEKAAGMILEDGR

Sequences:

>Translated_539_residues
MPRQLEGEYDYIVVGAGTAGCILANRLSADPKTRVLILEAGGYDNWIWFHIPVGYLFAIGNPRSDWMFRTESEPGLNGRS
LAYPRGKVIGGCSAINAMISMRGQAADYDHWRQLGLPGWSYSDVLAVFRRLEDHFLGESEHHGVGGGWRIEAPRLSWAVL
DAVGDAAEQMGIRRIPDFNTGDNEGISYFHVNQRRGRRWSSARGFLKPALKRQNLRLETNVLVDRLIVENGRVTGVRFQQ
DGQAIEARAKGEVILSAGSIGSVQVLQRSGIGPAEWLGELGIDVVLDKPGVGRNLQDHLQQRAIYKVSGVKTLNETYYSL
VKRGLMGLDYAFRRRGPLTMAPSQLGIFTRSDPRRDRANIQFHVQPLSLDKFGDPLHRFPAITVSACNLRPTSRGTVRIR
SAKIDEAPSIAPNYLATEDDREVAADAIRITRRLMKQSALAAYRPEEYLPGPSVGDDDAALAKAAGDIGTTIFHPVGTAK
MGTADDPMAVVDERLRFRGLDGLRIADASIMPTITSGNTNTPTAMIAEKAAGMILEDGR
>Mature_538_residues
PRQLEGEYDYIVVGAGTAGCILANRLSADPKTRVLILEAGGYDNWIWFHIPVGYLFAIGNPRSDWMFRTESEPGLNGRSL
AYPRGKVIGGCSAINAMISMRGQAADYDHWRQLGLPGWSYSDVLAVFRRLEDHFLGESEHHGVGGGWRIEAPRLSWAVLD
AVGDAAEQMGIRRIPDFNTGDNEGISYFHVNQRRGRRWSSARGFLKPALKRQNLRLETNVLVDRLIVENGRVTGVRFQQD
GQAIEARAKGEVILSAGSIGSVQVLQRSGIGPAEWLGELGIDVVLDKPGVGRNLQDHLQQRAIYKVSGVKTLNETYYSLV
KRGLMGLDYAFRRRGPLTMAPSQLGIFTRSDPRRDRANIQFHVQPLSLDKFGDPLHRFPAITVSACNLRPTSRGTVRIRS
AKIDEAPSIAPNYLATEDDREVAADAIRITRRLMKQSALAAYRPEEYLPGPSVGDDDAALAKAAGDIGTTIFHPVGTAKM
GTADDPMAVVDERLRFRGLDGLRIADASIMPTITSGNTNTPTAMIAEKAAGMILEDGR

Specific function: Converts aliphatic medium-chain-length alcohols into aldehydes. May be linked to the electron transfer chain [H]

COG id: COG2303

COG function: function code E; Choline dehydrogenase and related flavoproteins

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GMC oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI217272839, Length=537, Percent_Identity=40.4096834264432, Blast_Score=379, Evalue=1e-105,
Organism=Escherichia coli, GI1786503, Length=536, Percent_Identity=39.365671641791, Blast_Score=332, Evalue=5e-92,
Organism=Caenorhabditis elegans, GI17532301, Length=551, Percent_Identity=31.5789473684211, Blast_Score=292, Evalue=2e-79,
Organism=Drosophila melanogaster, GI24642055, Length=583, Percent_Identity=37.0497427101201, Blast_Score=310, Evalue=2e-84,
Organism=Drosophila melanogaster, GI24642042, Length=575, Percent_Identity=35.6521739130435, Blast_Score=308, Evalue=8e-84,
Organism=Drosophila melanogaster, GI24642048, Length=578, Percent_Identity=34.2560553633218, Blast_Score=293, Evalue=2e-79,
Organism=Drosophila melanogaster, GI24642039, Length=572, Percent_Identity=35.1398601398601, Blast_Score=283, Evalue=3e-76,
Organism=Drosophila melanogaster, GI18859995, Length=564, Percent_Identity=36.1702127659575, Blast_Score=280, Evalue=3e-75,
Organism=Drosophila melanogaster, GI45551458, Length=576, Percent_Identity=32.1180555555556, Blast_Score=274, Evalue=1e-73,
Organism=Drosophila melanogaster, GI45549471, Length=576, Percent_Identity=32.1180555555556, Blast_Score=273, Evalue=2e-73,
Organism=Drosophila melanogaster, GI17137792, Length=565, Percent_Identity=34.5132743362832, Blast_Score=268, Evalue=8e-72,
Organism=Drosophila melanogaster, GI24642059, Length=577, Percent_Identity=33.9688041594454, Blast_Score=265, Evalue=6e-71,
Organism=Drosophila melanogaster, GI24650267, Length=568, Percent_Identity=33.4507042253521, Blast_Score=252, Evalue=4e-67,
Organism=Drosophila melanogaster, GI24642051, Length=579, Percent_Identity=31.6062176165803, Blast_Score=244, Evalue=2e-64,
Organism=Drosophila melanogaster, GI18859993, Length=577, Percent_Identity=32.9289428076257, Blast_Score=242, Evalue=5e-64,
Organism=Drosophila melanogaster, GI24642035, Length=583, Percent_Identity=32.0754716981132, Blast_Score=234, Evalue=1e-61,
Organism=Drosophila melanogaster, GI24642037, Length=301, Percent_Identity=36.5448504983389, Blast_Score=171, Evalue=1e-42,
Organism=Drosophila melanogaster, GI24642057, Length=583, Percent_Identity=29.5025728987993, Blast_Score=161, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24645930, Length=595, Percent_Identity=28.0672268907563, Blast_Score=152, Evalue=5e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012132
- InterPro:   IPR000172
- InterPro:   IPR007867 [H]

Pfam domain/function: PF05199 GMC_oxred_C; PF00732 GMC_oxred_N [H]

EC number: 1.1.99.1 [C]

Molecular weight: Translated: 59187; Mature: 59056

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRQLEGEYDYIVVGAGTAGCILANRLSADPKTRVLILEAGGYDNWIWFHIPVGYLFAIG
CCCCCCCCCCEEEEECCCCHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEECCEEEEEEC
NPRSDWMFRTESEPGLNGRSLAYPRGKVIGGCSAINAMISMRGQAADYDHWRQLGLPGWS
CCCCCCEEEECCCCCCCCCEECCCCCCEEECHHHHHHHHHHCCCCCCHHHHHHHCCCCCC
YSDVLAVFRRLEDHFLGESEHHGVGGGWRIEAPRLSWAVLDAVGDAAEQMGIRRIPDFNT
HHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCC
GDNEGISYFHVNQRRGRRWSSARGFLKPALKRQNLRLETNVLVDRLIVENGRVTGVRFQQ
CCCCCEEEEEECHHCCCCCHHHCHHHHHHHHHCCCEEHHHHHHHHHHHCCCCEEEEEECC
DGQAIEARAKGEVILSAGSIGSVQVLQRSGIGPAEWLGELGIDVVLDKPGVGRNLQDHLQ
CCCCEECCCCCCEEEECCCCCCHHHHHHCCCCHHHHHHHCCCEEEECCCCCCCCHHHHHH
QRAIYKVSGVKTLNETYYSLVKRGLMGLDYAFRRRGPLTMAPSQLGIFTRSDPRRDRANI
HHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCEECCCCCEEEECCCCCCCCCCE
QFHVQPLSLDKFGDPLHRFPAITVSACNLRPTSRGTVRIRSAKIDEAPSIAPNYLATEDD
EEEEEEECHHHCCCHHHHCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCC
REVAADAIRITRRLMKQSALAAYRPEEYLPGPSVGDDDAALAKAAGDIGTTIFHPVGTAK
HHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCHHHHHHHHCCCCCHHCCCCCCCC
MGTADDPMAVVDERLRFRGLDGLRIADASIMPTITSGNTNTPTAMIAEKAAGMILEDGR
CCCCCCCHHHHHHHHHHCCCCCCEEECCCCCEEEECCCCCCCHHHHHHHHCCCEEECCC
>Mature Secondary Structure 
PRQLEGEYDYIVVGAGTAGCILANRLSADPKTRVLILEAGGYDNWIWFHIPVGYLFAIG
CCCCCCCCCEEEEECCCCHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEECCEEEEEEC
NPRSDWMFRTESEPGLNGRSLAYPRGKVIGGCSAINAMISMRGQAADYDHWRQLGLPGWS
CCCCCCEEEECCCCCCCCCEECCCCCCEEECHHHHHHHHHHCCCCCCHHHHHHHCCCCCC
YSDVLAVFRRLEDHFLGESEHHGVGGGWRIEAPRLSWAVLDAVGDAAEQMGIRRIPDFNT
HHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCC
GDNEGISYFHVNQRRGRRWSSARGFLKPALKRQNLRLETNVLVDRLIVENGRVTGVRFQQ
CCCCCEEEEEECHHCCCCCHHHCHHHHHHHHHCCCEEHHHHHHHHHHHCCCCEEEEEECC
DGQAIEARAKGEVILSAGSIGSVQVLQRSGIGPAEWLGELGIDVVLDKPGVGRNLQDHLQ
CCCCEECCCCCCEEEECCCCCCHHHHHHCCCCHHHHHHHCCCEEEECCCCCCCCHHHHHH
QRAIYKVSGVKTLNETYYSLVKRGLMGLDYAFRRRGPLTMAPSQLGIFTRSDPRRDRANI
HHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCEECCCCCEEEECCCCCCCCCCE
QFHVQPLSLDKFGDPLHRFPAITVSACNLRPTSRGTVRIRSAKIDEAPSIAPNYLATEDD
EEEEEEECHHHCCCHHHHCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCC
REVAADAIRITRRLMKQSALAAYRPEEYLPGPSVGDDDAALAKAAGDIGTTIFHPVGTAK
HHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCHHHHHHHHCCCCCHHCCCCCCCC
MGTADDPMAVVDERLRFRGLDGLRIADASIMPTITSGNTNTPTAMIAEKAAGMILEDGR
CCCCCCCHHHHHHHHHHCCCCCCEEECCCCCEEEECCCCCCCHHHHHHHHCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Coenzyme Q [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 7 {choline}} 1.1 {phenazine}} [C]

Substrates: Choline; Acceptor [C]

Specific reaction: Choline + Acceptor = Betaine Aldehyde + Reduced Acceptor. [C]

General reaction: Redox reaction [C]

Inhibitor: 2-Dimethylaminoethanol; Betaine aldehyde; Monoethanolamine; Semicarbazide [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11390693 [H]