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The map label for this gene is potH [H]

Identifier: 146339554

GI number: 146339554

Start: 2719414

End: 2720367

Strand: Reverse

Name: potH [H]

Synonym: BRADO2545

Alternate gene names: 146339554

Gene position: 2720367-2719414 (Counterclockwise)

Preceding gene: 146339555

Following gene: 146339553

Centisome position: 36.48

GC content: 62.68

Gene sequence:

>954_bases
TTGACGACAGGTATTGCCGTGGCCGTGACGATCGACAATTCGCCCGACACCATCGCGCCCGCGGAGAGCACGTTGCGCAA
GCTCACGATGCGGTCGATCGCCTGGCTGCAGGCCGGGCCGATGATGCTGGTGTTCGCGCTGTTCTTCCTGCTGCCGCTGG
TGCTCGTCGTCGTCGTCAGCGCCTGGGACTACAACGAATACGAGATGATCCCGGCGTTCAGCTTCCGCGGCTACACGGAC
ACGTTCGAGGGCTGTGTCGCCAACCTGCCCGAGCTGTGCACCATCCTGAAGACCTATCTGAAGACGCTGAAGCTCTGCCT
GCTCACCTGGAGCCTCACGCTGCTGATCGGGTTCTGGGTCGCCTATTTCCTCGCCTTCCACGTCCGCAGCAAGACCTGGC
AGATCGTGCTGTCGCTGTTGTGCACGATCCCGTTCTGGACCTCCAACGTGATCCGGATGATCGCCTGGATCCCCCTGCTC
GGCCGCAACGGCCTGGTCAACCAGGGCCTCGTCGGCGCTGGCGTCATCAACAAGCCGGTCGAGTGGCTGCTGTTCTCGGA
ATTCTCGGTGGTGCTGGCGCTGGTGCACCTGTTCACCTTCTTCATGGTGGTGCCGATCTTCAATTCGATGATCCGCATCG
ACAAGCGGCTGATCGAGGCCGCCTATGACGCCGGTGCGTCGGGCTGGCAGACGCTGGTCAACATCGTGATTCCCTTGTCG
AAGCCGGGCATCGTGATCGGCTCGATCTTCGTCATCACCATCGTGATGGGCGATTTCGTCACCATCGGCGTGATGGGCGG
CCAGCAGATCGCCTCGGCCGGCAAGATCATCGAGGCGCGCCTCTCGGCGCTGCAATTCCCGGCCGCCGCGGCGAATGCCG
TGATCCTGCTCGGCATCACCATCATCATCATCTCCGCGCTGACGCGGATCGTCGACGTGCGCAAGGAGCTCTGA

Upstream 100 bases:

>100_bases
TCCGCAAGTGGAACGAGTTCATCGCCGCCTGATGTGACCACCATAGCTGCCGCGCCCGCGATGGTCGTGGGCGCGGCAGA
TCTTCTCAGTCATCTGCGAC

Downstream 100 bases:

>100_bases
GAGATGGGAGACAAGCGCCCCCGCTCCTTCTACATCCTCGCGGCGTTCTTCTCGGCCTATGTGCTGTTCCTGTACGGGCC
GATGATCGCGATCTACATCC

Product: ABC transporter permease

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MTTGIAVAVTIDNSPDTIAPAESTLRKLTMRSIAWLQAGPMMLVFALFFLLPLVLVVVVSAWDYNEYEMIPAFSFRGYTD
TFEGCVANLPELCTILKTYLKTLKLCLLTWSLTLLIGFWVAYFLAFHVRSKTWQIVLSLLCTIPFWTSNVIRMIAWIPLL
GRNGLVNQGLVGAGVINKPVEWLLFSEFSVVLALVHLFTFFMVVPIFNSMIRIDKRLIEAAYDAGASGWQTLVNIVIPLS
KPGIVIGSIFVITIVMGDFVTIGVMGGQQIASAGKIIEARLSALQFPAAAANAVILLGITIIIISALTRIVDVRKEL

Sequences:

>Translated_317_residues
MTTGIAVAVTIDNSPDTIAPAESTLRKLTMRSIAWLQAGPMMLVFALFFLLPLVLVVVVSAWDYNEYEMIPAFSFRGYTD
TFEGCVANLPELCTILKTYLKTLKLCLLTWSLTLLIGFWVAYFLAFHVRSKTWQIVLSLLCTIPFWTSNVIRMIAWIPLL
GRNGLVNQGLVGAGVINKPVEWLLFSEFSVVLALVHLFTFFMVVPIFNSMIRIDKRLIEAAYDAGASGWQTLVNIVIPLS
KPGIVIGSIFVITIVMGDFVTIGVMGGQQIASAGKIIEARLSALQFPAAAANAVILLGITIIIISALTRIVDVRKEL
>Mature_316_residues
TTGIAVAVTIDNSPDTIAPAESTLRKLTMRSIAWLQAGPMMLVFALFFLLPLVLVVVVSAWDYNEYEMIPAFSFRGYTDT
FEGCVANLPELCTILKTYLKTLKLCLLTWSLTLLIGFWVAYFLAFHVRSKTWQIVLSLLCTIPFWTSNVIRMIAWIPLLG
RNGLVNQGLVGAGVINKPVEWLLFSEFSVVLALVHLFTFFMVVPIFNSMIRIDKRLIEAAYDAGASGWQTLVNIVIPLSK
PGIVIGSIFVITIVMGDFVTIGVMGGQQIASAGKIIEARLSALQFPAAAANAVILLGITIIIISALTRIVDVRKEL

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=209, Percent_Identity=35.4066985645933, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1787369, Length=222, Percent_Identity=33.3333333333333, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1787713, Length=177, Percent_Identity=25.9887005649718, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 34860; Mature: 34729

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTGIAVAVTIDNSPDTIAPAESTLRKLTMRSIAWLQAGPMMLVFALFFLLPLVLVVVVS
CCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
AWDYNEYEMIPAFSFRGYTDTFEGCVANLPELCTILKTYLKTLKLCLLTWSLTLLIGFWV
CCCCCCCEECCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AYFLAFHVRSKTWQIVLSLLCTIPFWTSNVIRMIAWIPLLGRNGLVNQGLVGAGVINKPV
HHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCHH
EWLLFSEFSVVLALVHLFTFFMVVPIFNSMIRIDKRLIEAAYDAGASGWQTLVNIVIPLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
KPGIVIGSIFVITIVMGDFVTIGVMGGQQIASAGKIIEARLSALQFPAAAANAVILLGIT
CCCHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
IIIISALTRIVDVRKEL
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTGIAVAVTIDNSPDTIAPAESTLRKLTMRSIAWLQAGPMMLVFALFFLLPLVLVVVVS
CCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH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HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]