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The map label for this gene is wcaJ [H]

Identifier: 146339261

GI number: 146339261

Start: 2379127

End: 2380596

Strand: Direct

Name: wcaJ [H]

Synonym: BRADO2232

Alternate gene names: 146339261

Gene position: 2379127-2380596 (Clockwise)

Preceding gene: 146339260

Following gene: 146339262

Centisome position: 31.91

GC content: 63.95

Gene sequence:

>1470_bases
GTGCTCGACATGGGGCGTGATGTGTCGGATCTGACGGGAATTGGACAGTCCAGGGCGGCCTTCGACCAGCGCGTCTGGCC
GCGGCTGCGGGTTGGCTTCGAGGTGATCGGGCCGCTGTTTGCAGCCCTCGATGTGGTGACCGTCATCGTATCGGGCTTGC
TCGGAGGCTTGGGTTACCACGCGGCGATCGGCGAGGGTCTGGGCGAGGTCACGGTCCATCTCGGCCTCGGCATGATTGCC
GGGTTCTGTCACTGCGCCGTCGCCAACCACATTGGACTCTACGCGCATGATCGCCTGCTGGAAGGTCAGCAGGACAAACG
CCGCGTGCTGGCAAGCTGGGCGCTCGCCATTCTCATTCTCACGGTCGCGCTGTTCTTGCTGAAATCAAGTGCCGACATCT
CGCGCGGCACCATGATCTGCGTCTTTCTTCTAGGCGGTGTGGCGCTGCCGCTGGTCCGGCGGGTGGCGCAGTGGCGCCTG
CGCTCAGCCATCCGGCGAGGGGACATCAAAGGGCGAATCGTGCTCGTGATGGGAACGCGGTCCGAATTGGCGCAGCTCTC
GCGGGCGGAATTGCTGTTGCGGTTCGGACTGGACGAGCTCGGTCGCATCGTTCTGCCGGACACGGCCGGAGCGCGCGGGG
AGGCCAGCACGCGCGACGATCTCATCGCCGCGTTGCGCAGGCAACCGGTGGAAGAGATCATCGTCGCGCTTCCCTGGTCG
CAACTCGGCGAGTTCGACTGGCTGCTCGCCTGCCTGCGGGCGGTGCCGTTGCCGGTTCGGTTGTTGCCCGACGAGGCGGT
CTCCTCCGTGGTGCGGCGGCAGACCGCGCAGCCGCAATACGCCTATATCGTCGAGATGCAGCGGGCCCCGCTCAGCGGGC
TCGAAAGGTGCGCCAAACGCATTCTCGACATCGTCGTCGCGGTCACGTCGTTGATGGCTCTGTCGCCGCTGCTGCTGCTC
GCCACCCTCGCGGTGAAGCTCGAGAGTGCCGGCCCCGTCATCTTTCGGCAGCGCCGGCGAGGCTTCAACGGCGAAACCTT
CATGATCTACAAGTTCCGGTCGATGACGGTGATGGAGGATGGCGCCGCGGTGCTTCAGGCGCAACGGCATGATCCCCGTG
TCACGCAGACCGGGAGGTTTCTGCGGGCTACCAGCATCGACGAGATTCCGCAGCTTTGGAACGTGCTGCGCGGCGAGATG
TCCATCGTGGGCCCACGGCCCCACGCCCTCGCGCATGACGATGAATACGGCAAGATGATCGCCAATTATGCACTCCGCCA
CCACGTCAAGCCCGGCATGACCGGCTGGGCTCAGGTCAACGGGTTGCGCGGCGGGACGCCGCGGCTCGAAATGATGCAAC
AGCGTGTCGCACTGGATTTATGGTACATTGATCACTGGAGTCTTTCGCTCGATGTCTATATCATGATACGTACTGCCTTT
GAGCTTATGCGGCACCGAAATGCCTATTGA

Upstream 100 bases:

>100_bases
CGTGGCGCGGCCGCCGGCCATGTAACATCGGGCGGTGCAATTCGCGCGATGTTAACCAACAATCATGATAATGTTATCTC
AGATATGATACGTCCCGTCG

Downstream 100 bases:

>100_bases
CGGCGTTATCGGCGAGCTGAATGACCGTAGAACGATATGGATAGCGCCGGCCGGATGTGAACCATCCGCGAGGAACCGGC
TCGATACCGCGGGCCAGATG

Product: putative UDP-glucose lipid carrier transferase membrane protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 489; Mature: 489

Protein sequence:

>489_residues
MLDMGRDVSDLTGIGQSRAAFDQRVWPRLRVGFEVIGPLFAALDVVTVIVSGLLGGLGYHAAIGEGLGEVTVHLGLGMIA
GFCHCAVANHIGLYAHDRLLEGQQDKRRVLASWALAILILTVALFLLKSSADISRGTMICVFLLGGVALPLVRRVAQWRL
RSAIRRGDIKGRIVLVMGTRSELAQLSRAELLLRFGLDELGRIVLPDTAGARGEASTRDDLIAALRRQPVEEIIVALPWS
QLGEFDWLLACLRAVPLPVRLLPDEAVSSVVRRQTAQPQYAYIVEMQRAPLSGLERCAKRILDIVVAVTSLMALSPLLLL
ATLAVKLESAGPVIFRQRRRGFNGETFMIYKFRSMTVMEDGAAVLQAQRHDPRVTQTGRFLRATSIDEIPQLWNVLRGEM
SIVGPRPHALAHDDEYGKMIANYALRHHVKPGMTGWAQVNGLRGGTPRLEMMQQRVALDLWYIDHWSLSLDVYIMIRTAF
ELMRHRNAY

Sequences:

>Translated_489_residues
MLDMGRDVSDLTGIGQSRAAFDQRVWPRLRVGFEVIGPLFAALDVVTVIVSGLLGGLGYHAAIGEGLGEVTVHLGLGMIA
GFCHCAVANHIGLYAHDRLLEGQQDKRRVLASWALAILILTVALFLLKSSADISRGTMICVFLLGGVALPLVRRVAQWRL
RSAIRRGDIKGRIVLVMGTRSELAQLSRAELLLRFGLDELGRIVLPDTAGARGEASTRDDLIAALRRQPVEEIIVALPWS
QLGEFDWLLACLRAVPLPVRLLPDEAVSSVVRRQTAQPQYAYIVEMQRAPLSGLERCAKRILDIVVAVTSLMALSPLLLL
ATLAVKLESAGPVIFRQRRRGFNGETFMIYKFRSMTVMEDGAAVLQAQRHDPRVTQTGRFLRATSIDEIPQLWNVLRGEM
SIVGPRPHALAHDDEYGKMIANYALRHHVKPGMTGWAQVNGLRGGTPRLEMMQQRVALDLWYIDHWSLSLDVYIMIRTAF
ELMRHRNAY
>Mature_489_residues
MLDMGRDVSDLTGIGQSRAAFDQRVWPRLRVGFEVIGPLFAALDVVTVIVSGLLGGLGYHAAIGEGLGEVTVHLGLGMIA
GFCHCAVANHIGLYAHDRLLEGQQDKRRVLASWALAILILTVALFLLKSSADISRGTMICVFLLGGVALPLVRRVAQWRL
RSAIRRGDIKGRIVLVMGTRSELAQLSRAELLLRFGLDELGRIVLPDTAGARGEASTRDDLIAALRRQPVEEIIVALPWS
QLGEFDWLLACLRAVPLPVRLLPDEAVSSVVRRQTAQPQYAYIVEMQRAPLSGLERCAKRILDIVVAVTSLMALSPLLLL
ATLAVKLESAGPVIFRQRRRGFNGETFMIYKFRSMTVMEDGAAVLQAQRHDPRVTQTGRFLRATSIDEIPQLWNVLRGEM
SIVGPRPHALAHDDEYGKMIANYALRHHVKPGMTGWAQVNGLRGGTPRLEMMQQRVALDLWYIDHWSLSLDVYIMIRTAF
ELMRHRNAY

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=437, Percent_Identity=34.0961098398169, Blast_Score=238, Evalue=5e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 54280; Mature: 54280

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDMGRDVSDLTGIGQSRAAFDQRVWPRLRVGFEVIGPLFAALDVVTVIVSGLLGGLGYH
CCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAIGEGLGEVTVHLGLGMIAGFCHCAVANHIGLYAHDRLLEGQQDKRRVLASWALAILIL
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHH
TVALFLLKSSADISRGTMICVFLLGGVALPLVRRVAQWRLRSAIRRGDIKGRIVLVMGTR
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCH
SELAQLSRAELLLRFGLDELGRIVLPDTAGARGEASTRDDLIAALRRQPVEEIIVALPWS
HHHHHHHHHHHHHHHCHHHHCCEEECCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHCCHH
QLGEFDWLLACLRAVPLPVRLLPDEAVSSVVRRQTAQPQYAYIVEMQRAPLSGLERCAKR
HHCCHHHHHHHHHHCCCCEEECCHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHH
ILDIVVAVTSLMALSPLLLLATLAVKLESAGPVIFRQRRRGFNGETFMIYKFRSMTVMED
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCEEEEEEECCCEEECC
GAAVLQAQRHDPRVTQTGRFLRATSIDEIPQLWNVLRGEMSIVGPRPHALAHDDEYGKMI
CHHHHHHHCCCCCCHHCCCCHHHCCHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHH
ANYALRHHVKPGMTGWAQVNGLRGGTPRLEMMQQRVALDLWYIDHWSLSLDVYIMIRTAF
HHHHHHHCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHH
ELMRHRNAY
HHHHHCCCC
>Mature Secondary Structure
MLDMGRDVSDLTGIGQSRAAFDQRVWPRLRVGFEVIGPLFAALDVVTVIVSGLLGGLGYH
CCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAIGEGLGEVTVHLGLGMIAGFCHCAVANHIGLYAHDRLLEGQQDKRRVLASWALAILIL
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHH
TVALFLLKSSADISRGTMICVFLLGGVALPLVRRVAQWRLRSAIRRGDIKGRIVLVMGTR
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCH
SELAQLSRAELLLRFGLDELGRIVLPDTAGARGEASTRDDLIAALRRQPVEEIIVALPWS
HHHHHHHHHHHHHHHCHHHHCCEEECCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHCCHH
QLGEFDWLLACLRAVPLPVRLLPDEAVSSVVRRQTAQPQYAYIVEMQRAPLSGLERCAKR
HHCCHHHHHHHHHHCCCCEEECCHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHH
ILDIVVAVTSLMALSPLLLLATLAVKLESAGPVIFRQRRRGFNGETFMIYKFRSMTVMED
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCEEEEEEECCCEEECC
GAAVLQAQRHDPRVTQTGRFLRATSIDEIPQLWNVLRGEMSIVGPRPHALAHDDEYGKMI
CHHHHHHHCCCCCCHHCCCCHHHCCHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHH
ANYALRHHVKPGMTGWAQVNGLRGGTPRLEMMQQRVALDLWYIDHWSLSLDVYIMIRTAF
HHHHHHHCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHH
ELMRHRNAY
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]