Click here to switch to the map view.

The map label for this gene is gmd [H]

Identifier: 146339220

GI number: 146339220

Start: 2318493

End: 2319551

Strand: Reverse

Name: gmd [H]

Synonym: BRADO2185

Alternate gene names: 146339220

Gene position: 2319551-2318493 (Counterclockwise)

Preceding gene: 146339221

Following gene: 146339219

Centisome position: 31.11

GC content: 61.38

Gene sequence:

>1059_bases
GTGGCTCTGATCACGGGCGTCACCGGACAGGACGGCGCCTATCTTTCCGAATACCTTTTAGGCCTTGGCTACACCGTCCA
CGGCATCAAACGGCGGTCGTCGTCCTTCAATACGGCGCGCATCGATCATCTCTATGAGGACCGGCACGAAGGCAACACGC
CCTTCCTGCTGCACTACGGAGATATGACCGACTCGACTAATCTTATTCGGCTGGTGCAGCAGATCCGTCCCACCGAAATC
TACAATCTGGCGGCGCAAAGTCATGTCGGCGTCAGCTTCGAGAGCCCTGAATACACAGCCAATTCAGATGCGCTTGGCGT
GCTGCGCCTGCTCGAGGCCATCCGCATTCTCGGCATGGAGCGCGAGACTCGGTTCTACCAGGCATCCACATCCGAGCTCT
ACGGGCTTGTCCAGGAGACACCGCAGCGGGAAAGCACGCCCTTTTATCCGCGATCGCCCTACGGCTGCGCCAAGCTGTAC
GGTTACTGGATCACGGTGAACTATCGCGAAGCCTATGGAATGTTCGCGGCGAACGGCATCCTGTTCAACCATGAAAGCCC
GGTCCGCGGCGAGACGTTCGTGACGAGAAAGATCACGCGGGCGGTGGCGCGGATCGAGATGGGCCTGGAGAGCACCCTGT
ATCTCGGCAATCTCGAGGCAAAGCGCGACTGGGGACATGCCCGGGACTACGTCGAGGGCATGCACCAGATCCTCCAGATG
GACGAGCCCGATGATTTCGTTTTGGCGACCGGCGAGACGCGCTCGGTGCGCGAGTTCGTCGAGCTTGCCTTTGCCGAGGT
CGGTCGCCAGATCGAGTGGAGCGGCCGCGAGATCGAGGAAGTCGGGGTCGACCGCGAATCCGGACAAACCGTCGTTCGCA
TCGACCCGCGCTATTTCCGCCCGACCGAGGTGGATCTTCTGGTCGGCGATGCCAGCAAGGCGCGCGACAAGCTTGGTTGG
CGGCCGAAGCGAAGTCTGAGCGAGCTGATCCGCGAAATGGTCGCAAACGATCTGCAAGTCGCCCGGGGGGAGGTCGCCCA
TGGCTCCCGCCACCTTTGA

Upstream 100 bases:

>100_bases
AGTATCATGTCCATGTTATACTTCGATGTTACCGAGCGCTGCGCGGCGTGCGGGGCCGACGGACTTAAAGGGCGGATGCG
ACTTGAGTTCCAAACAGCCA

Downstream 100 bases:

>100_bases
TCTCGCCCACAAGCGCGTCTTCGTGGCTGGCCATCGCGGACTGGTCGGCAGCGCGCTGGTGCGGCGCTTGGCAAGCGAGG
GCGTCGAACTGCAGACCGTG

Product: GDP-D-mannose dehydratase, NAD(P)-binding, colanic acid synthesis

Products: NA

Alternate protein names: GDP-D-mannose dehydratase [H]

Number of amino acids: Translated: 352; Mature: 351

Protein sequence:

>352_residues
MALITGVTGQDGAYLSEYLLGLGYTVHGIKRRSSSFNTARIDHLYEDRHEGNTPFLLHYGDMTDSTNLIRLVQQIRPTEI
YNLAAQSHVGVSFESPEYTANSDALGVLRLLEAIRILGMERETRFYQASTSELYGLVQETPQRESTPFYPRSPYGCAKLY
GYWITVNYREAYGMFAANGILFNHESPVRGETFVTRKITRAVARIEMGLESTLYLGNLEAKRDWGHARDYVEGMHQILQM
DEPDDFVLATGETRSVREFVELAFAEVGRQIEWSGREIEEVGVDRESGQTVVRIDPRYFRPTEVDLLVGDASKARDKLGW
RPKRSLSELIREMVANDLQVARGEVAHGSRHL

Sequences:

>Translated_352_residues
MALITGVTGQDGAYLSEYLLGLGYTVHGIKRRSSSFNTARIDHLYEDRHEGNTPFLLHYGDMTDSTNLIRLVQQIRPTEI
YNLAAQSHVGVSFESPEYTANSDALGVLRLLEAIRILGMERETRFYQASTSELYGLVQETPQRESTPFYPRSPYGCAKLY
GYWITVNYREAYGMFAANGILFNHESPVRGETFVTRKITRAVARIEMGLESTLYLGNLEAKRDWGHARDYVEGMHQILQM
DEPDDFVLATGETRSVREFVELAFAEVGRQIEWSGREIEEVGVDRESGQTVVRIDPRYFRPTEVDLLVGDASKARDKLGW
RPKRSLSELIREMVANDLQVARGEVAHGSRHL
>Mature_351_residues
ALITGVTGQDGAYLSEYLLGLGYTVHGIKRRSSSFNTARIDHLYEDRHEGNTPFLLHYGDMTDSTNLIRLVQQIRPTEIY
NLAAQSHVGVSFESPEYTANSDALGVLRLLEAIRILGMERETRFYQASTSELYGLVQETPQRESTPFYPRSPYGCAKLYG
YWITVNYREAYGMFAANGILFNHESPVRGETFVTRKITRAVARIEMGLESTLYLGNLEAKRDWGHARDYVEGMHQILQMD
EPDDFVLATGETRSVREFVELAFAEVGRQIEWSGREIEEVGVDRESGQTVVRIDPRYFRPTEVDLLVGDASKARDKLGWR
PKRSLSELIREMVANDLQVARGEVAHGSRHL

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=345, Percent_Identity=60.8695652173913, Blast_Score=453, Evalue=1e-127,
Organism=Escherichia coli, GI1788366, Length=352, Percent_Identity=62.7840909090909, Blast_Score=483, Evalue=1e-137,
Organism=Escherichia coli, GI48994969, Length=292, Percent_Identity=26.3698630136986, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788353, Length=299, Percent_Identity=24.0802675585284, Blast_Score=65, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17539424, Length=340, Percent_Identity=64.1176470588235, Blast_Score=466, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI133901790, Length=340, Percent_Identity=64.1176470588235, Blast_Score=466, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI133901788, Length=340, Percent_Identity=64.1176470588235, Blast_Score=465, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI17539422, Length=340, Percent_Identity=64.1176470588235, Blast_Score=465, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI133901786, Length=340, Percent_Identity=64.1176470588235, Blast_Score=465, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI17507723, Length=346, Percent_Identity=63.2947976878613, Blast_Score=460, Evalue=1e-130,
Organism=Drosophila melanogaster, GI24158427, Length=344, Percent_Identity=62.5, Blast_Score=459, Evalue=1e-129,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 39982; Mature: 39850

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALITGVTGQDGAYLSEYLLGLGYTVHGIKRRSSSFNTARIDHLYEDRHEGNTPFLLHYG
CEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEEC
DMTDSTNLIRLVQQIRPTEIYNLAAQSHVGVSFESPEYTANSDALGVLRLLEAIRILGME
CCCCCHHHHHHHHHHCCHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHCCC
RETRFYQASTSELYGLVQETPQRESTPFYPRSPYGCAKLYGYWITVNYREAYGMFAANGI
HHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHEEEEEEEEEEEHHHHCEEEECCE
LFNHESPVRGETFVTRKITRAVARIEMGLESTLYLGNLEAKRDWGHARDYVEGMHQILQM
EECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHCCCHHHHHHHHHHHHHCC
DEPDDFVLATGETRSVREFVELAFAEVGRQIEWSGREIEEVGVDRESGQTVVRIDPRYFR
CCCCCEEEECCCCHHHHHHHHHHHHHHCCEEECCCCCHHHHCCCCCCCCEEEEECCCCCC
PTEVDLLVGDASKARDKLGWRPKRSLSELIREMVANDLQVARGEVAHGSRHL
CCEEEEEECCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
ALITGVTGQDGAYLSEYLLGLGYTVHGIKRRSSSFNTARIDHLYEDRHEGNTPFLLHYG
EEEECCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEEC
DMTDSTNLIRLVQQIRPTEIYNLAAQSHVGVSFESPEYTANSDALGVLRLLEAIRILGME
CCCCCHHHHHHHHHHCCHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHCCC
RETRFYQASTSELYGLVQETPQRESTPFYPRSPYGCAKLYGYWITVNYREAYGMFAANGI
HHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHEEEEEEEEEEEHHHHCEEEECCE
LFNHESPVRGETFVTRKITRAVARIEMGLESTLYLGNLEAKRDWGHARDYVEGMHQILQM
EECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHCCCHHHHHHHHHHHHHCC
DEPDDFVLATGETRSVREFVELAFAEVGRQIEWSGREIEEVGVDRESGQTVVRIDPRYFR
CCCCCEEEECCCCHHHHHHHHHHHHHHCCEEECCCCCHHHHCCCCCCCCEEEEECCCCCC
PTEVDLLVGDASKARDKLGWRPKRSLSELIREMVANDLQVARGEVAHGSRHL
CCEEEEEECCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10065558 [H]