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The map label for this gene is fcl [H]

Identifier: 146339219

GI number: 146339219

Start: 2317556

End: 2318512

Strand: Reverse

Name: fcl [H]

Synonym: BRADO2184

Alternate gene names: 146339219

Gene position: 2318512-2317556 (Counterclockwise)

Preceding gene: 146339220

Following gene: 146339218

Centisome position: 31.09

GC content: 64.68

Gene sequence:

>957_bases
ATGGCTCCCGCCACCTTTGATCTCGCCCACAAGCGCGTCTTCGTGGCTGGCCATCGCGGACTGGTCGGCAGCGCGCTGGT
GCGGCGCTTGGCAAGCGAGGGCGTCGAACTGCAGACCGTGTCGCGTGGCGAGGTCGATCTGAGCGACCAGGCCGCGGTGT
TTGCTTGGTTCGCGCGCGCGAAGCCCCAGGTCGTGTTCCTCGCAGCGGCCAAGGTCGGCGGCATCGTCGCCAACAACACG
CTGCGCGCCGAATTCATCTACGACAACATCGCGATCGCCACCAACGTCATTCACGCCGCCCATGTGAACGGCGTCGAGAA
GCTGATGTTTCTCGGTTCGTCCTGCATCTACCCGAAGCTCGCGGCCCAGCCGCTGCGCGAGGATGCGATGCTGACCGGTC
CGCTGGAGCCGACCAACGAGCCCTATGCGATTGCCAAGATCGCAGGTATCAAGATGGTCGAGGCCTATCGCAGCCAGTAT
GGCGCCGACTTCATCAACGTCATGCCGACGAATCTCTATGGTCCCGGCGACAACTATCATCCCGAGTACAGCCACGTCGT
CGCGGCGTTGATCCGCCGCTTCCACGAGGCGAAGCTTGCGGACGCGCCGGAGGTCATCGTCTGGGGCACCGGCAAGCCGC
GGCGCGAATTCCTCTATGTCGACGACATGGCGGATGCCTGCGTGCACCTGATGAAGACATATTCGGAGCCCGGCCTCGTC
AACATCGGGACCGGAGAGGACATCAGCATCGCCGACTTCGCTGCAATGGTCGCGACAGCCGTCGGCTACGAGGGCCGGAT
CCGTTTCGACCCGTCGCGGCCCGACGGCACGCCGCGCAAGCTGCTCGACGTCGGCCGGCTGACGAGCCTTGGATGGTCGG
CGACAACCTCGCTTCAAGACGGTATCAGGGGAGCCTATCGGGCCTACCTCGCGGAGACGTCCCTCCAGCTGGTCTGA

Upstream 100 bases:

>100_bases
GCGCGCGACAAGCTTGGTTGGCGGCCGAAGCGAAGTCTGAGCGAGCTGATCCGCGAAATGGTCGCAAACGATCTGCAAGT
CGCCCGGGGGGAGGTCGCCC

Downstream 100 bases:

>100_bases
TCTGGGACAACAACGGCGCGGTGCCGTGCAGCGCTGTCGTGAAAGCTTGACTCGCGCGTCACCGCCGCGACACCCCATCA
TGGTACCACAACTACACGGT

Product: bifunctional GDP-fucose synthetase GDP-4-dehydro-6-deoxy-D-mannose epimerase/GDP-4-dehydro-6-L-deoxygalactose reductase

Products: NA

Alternate protein names: GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase [H]

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MAPATFDLAHKRVFVAGHRGLVGSALVRRLASEGVELQTVSRGEVDLSDQAAVFAWFARAKPQVVFLAAAKVGGIVANNT
LRAEFIYDNIAIATNVIHAAHVNGVEKLMFLGSSCIYPKLAAQPLREDAMLTGPLEPTNEPYAIAKIAGIKMVEAYRSQY
GADFINVMPTNLYGPGDNYHPEYSHVVAALIRRFHEAKLADAPEVIVWGTGKPRREFLYVDDMADACVHLMKTYSEPGLV
NIGTGEDISIADFAAMVATAVGYEGRIRFDPSRPDGTPRKLLDVGRLTSLGWSATTSLQDGIRGAYRAYLAETSLQLV

Sequences:

>Translated_318_residues
MAPATFDLAHKRVFVAGHRGLVGSALVRRLASEGVELQTVSRGEVDLSDQAAVFAWFARAKPQVVFLAAAKVGGIVANNT
LRAEFIYDNIAIATNVIHAAHVNGVEKLMFLGSSCIYPKLAAQPLREDAMLTGPLEPTNEPYAIAKIAGIKMVEAYRSQY
GADFINVMPTNLYGPGDNYHPEYSHVVAALIRRFHEAKLADAPEVIVWGTGKPRREFLYVDDMADACVHLMKTYSEPGLV
NIGTGEDISIADFAAMVATAVGYEGRIRFDPSRPDGTPRKLLDVGRLTSLGWSATTSLQDGIRGAYRAYLAETSLQLV
>Mature_317_residues
APATFDLAHKRVFVAGHRGLVGSALVRRLASEGVELQTVSRGEVDLSDQAAVFAWFARAKPQVVFLAAAKVGGIVANNTL
RAEFIYDNIAIATNVIHAAHVNGVEKLMFLGSSCIYPKLAAQPLREDAMLTGPLEPTNEPYAIAKIAGIKMVEAYRSQYG
ADFINVMPTNLYGPGDNYHPEYSHVVAALIRRFHEAKLADAPEVIVWGTGKPRREFLYVDDMADACVHLMKTYSEPGLVN
IGTGEDISIADFAAMVATAVGYEGRIRFDPSRPDGTPRKLLDVGRLTSLGWSATTSLQDGIRGAYRAYLAETSLQLV

Specific function: Two step NADP-dependent conversion of GDP-4-dehydro-6- deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fucose synthase family [H]

Homologues:

Organism=Homo sapiens, GI4507709, Length=282, Percent_Identity=34.0425531914894, Blast_Score=167, Evalue=9e-42,
Organism=Homo sapiens, GI7657641, Length=331, Percent_Identity=25.0755287009063, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI42516563, Length=322, Percent_Identity=26.3975155279503, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1788365, Length=313, Percent_Identity=57.5079872204473, Blast_Score=369, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17554428, Length=286, Percent_Identity=29.3706293706294, Blast_Score=137, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI115532424, Length=281, Percent_Identity=26.3345195729537, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI19922778, Length=312, Percent_Identity=33.6538461538462, Blast_Score=174, Evalue=5e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.271 [H]

Molecular weight: Translated: 34497; Mature: 34366

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPATFDLAHKRVFVAGHRGLVGSALVRRLASEGVELQTVSRGEVDLSDQAAVFAWFARA
CCCCCHHHHHCEEEEECCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHCC
KPQVVFLAAAKVGGIVANNTLRAEFIYDNIAIATNVIHAAHVNGVEKLMFLGSSCIYPKL
CCCEEEEEEECCCCEEECCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
AAQPLREDAMLTGPLEPTNEPYAIAKIAGIKMVEAYRSQYGADFINVMPTNLYGPGDNYH
HHCCCCCCCEEECCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCC
PEYSHVVAALIRRFHEAKLADAPEVIVWGTGKPRREFLYVDDMADACVHLMKTYSEPGLV
CCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCEEEEEECHHHHHHHHHHHHCCCCCEE
NIGTGEDISIADFAAMVATAVGYEGRIRFDPSRPDGTPRKLLDVGRLTSLGWSATTSLQD
ECCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHHHH
GIRGAYRAYLAETSLQLV
HHHHHHHHHHHHHHEECC
>Mature Secondary Structure 
APATFDLAHKRVFVAGHRGLVGSALVRRLASEGVELQTVSRGEVDLSDQAAVFAWFARA
CCCCHHHHHCEEEEECCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHCC
KPQVVFLAAAKVGGIVANNTLRAEFIYDNIAIATNVIHAAHVNGVEKLMFLGSSCIYPKL
CCCEEEEEEECCCCEEECCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
AAQPLREDAMLTGPLEPTNEPYAIAKIAGIKMVEAYRSQYGADFINVMPTNLYGPGDNYH
HHCCCCCCCEEECCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCC
PEYSHVVAALIRRFHEAKLADAPEVIVWGTGKPRREFLYVDDMADACVHLMKTYSEPGLV
CCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCEEEEEECHHHHHHHHHHHHCCCCCEE
NIGTGEDISIADFAAMVATAVGYEGRIRFDPSRPDGTPRKLLDVGRLTSLGWSATTSLQD
ECCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHHHH
GIRGAYRAYLAETSLQLV
HHHHHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7815923; 8759852; 9097040; 9278503; 9473059; 9862812 [H]