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The map label for this gene is ygcA [C]

Identifier: 146339198

GI number: 146339198

Start: 2293145

End: 2294380

Strand: Reverse

Name: ygcA [C]

Synonym: BRADO2158

Alternate gene names: 146339198

Gene position: 2294380-2293145 (Counterclockwise)

Preceding gene: 146339199

Following gene: 146339195

Centisome position: 30.77

GC content: 70.15

Gene sequence:

>1236_bases
ATGGTTGAGCGTCTCGTCATCGACCATGTCGGCCATCGCGGCGACGGCGTATCGCTGTCCTCGGGCGAGGCACTGTACGT
GCCGTATACGCTGGCTGGCGAGACCGTCGAGGCCGCGGCCATTCCCGGCCATCCCGACCGCCGCAAGCTGCTCGCGGTAG
AGGCACCGAGCCCGGAGCGCATCACGCCGTTCTGCCCGCATTTCGGCGTGTGCGGCGGCTGCGCCATCCAGCACTGGTCG
CCGGAGCCTTATCGGCAGTGGAAGCGCAACATCGTCGTCGAGACGCTGCGCGCCGCAAAGGTCGATTGCGAGGTGGCGCC
GCTGATCGACGCCCACGGCGCCGGCCGCCGCCGCGCGACGTTCCACGCGCGGATGGGCACGCACGATATTCTCAGGGTCG
GCTTCTCGGCCGCGAACAGCCACGAGATCGTCCCGATCGACCGCTGCCCGATCCTCGATCCCGCGCTCGATGGCGCGCTG
GAAGCTGCGTGGGCGCTGGCGGAGGCGTTGAAGCCGACCGGCAAGCCGCTCGACATCCAGGCCACCGCGACCAGCAACGG
TATCGACGTCGACATCCGCGGCTCGGGCCCGCTGCCGCCGCCGCGCATCGCGGCGCTCTCGCTGGTCGCCGAGAAGCACC
GGCTGGCACGGCTGACGCGGCATGGCGAGTTGGTGCTGATGCGTAATCCGCCCGTCATGACGATGGGCACGGCGCAGGTG
ACGCTGCCGCCCGGCTCGTTCCTGCAGGCGACCGCGCAGGGCGAGGAGACACTGGCGCAGCTGGTGCTGGATCGCGTCGG
CAAGGCGAAGCAGGTCGCCGACCTGTTCTGCGGCGTCGGCCCGTTCGCGCTGCGGCTGGCGTCGCGCGGGCGCGTCATCG
CGATGGACACTGACGCCGGCTCGATCGCAGCGCTGCAGAAGGCGGCGGGGACGCCTAGCCTGAAGCCGATCAAAGCCGAG
GCCCGCGACCTGTTCCGCCGCCCCCTAATGCCGCCGGAGCTGCGCGACATCGACGCCGTCGTGTTCGACCCGCCGCGCCA
GGGCGCCCAGGCACAAGCGAAGCAGCTCGCCGCGAGCAAGGTGCCGGTGATCGTCGCGGTGTCGTGCAACGTCACGACTT
TCGCCCGCGATGTGCGCATGCTGATCGACGGCGGCTACAAGCTCGAGACCGTGGTGCCCGTCGACCAGTTCCGCCACACG
CCGCATGTGGAACTGGTCGCGAGATTGGTACGGTGA

Upstream 100 bases:

>100_bases
GCATTTGCAGCCTCGCTCGGCTGTAGTGACATCGAGATCTTCCCGTCATCCATCAAGGGCGGCGACGGCAACGCCGAATT
CTTTCTGGGAGCCCGCCGCC

Downstream 100 bases:

>100_bases
GCTGACGCCCATTCAAGTCCTTGCGAGCGCAGCGAAGCAATCGAGAGTCGTTGCTGGGCCCTGGATTGCTTCGCTGCGCT
CGCAAGGACGAGGAGGCCGG

Product: putative RNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 411; Mature: 411

Protein sequence:

>411_residues
MVERLVIDHVGHRGDGVSLSSGEALYVPYTLAGETVEAAAIPGHPDRRKLLAVEAPSPERITPFCPHFGVCGGCAIQHWS
PEPYRQWKRNIVVETLRAAKVDCEVAPLIDAHGAGRRRATFHARMGTHDILRVGFSAANSHEIVPIDRCPILDPALDGAL
EAAWALAEALKPTGKPLDIQATATSNGIDVDIRGSGPLPPPRIAALSLVAEKHRLARLTRHGELVLMRNPPVMTMGTAQV
TLPPGSFLQATAQGEETLAQLVLDRVGKAKQVADLFCGVGPFALRLASRGRVIAMDTDAGSIAALQKAAGTPSLKPIKAE
ARDLFRRPLMPPELRDIDAVVFDPPRQGAQAQAKQLAASKVPVIVAVSCNVTTFARDVRMLIDGGYKLETVVPVDQFRHT
PHVELVARLVR

Sequences:

>Translated_411_residues
MVERLVIDHVGHRGDGVSLSSGEALYVPYTLAGETVEAAAIPGHPDRRKLLAVEAPSPERITPFCPHFGVCGGCAIQHWS
PEPYRQWKRNIVVETLRAAKVDCEVAPLIDAHGAGRRRATFHARMGTHDILRVGFSAANSHEIVPIDRCPILDPALDGAL
EAAWALAEALKPTGKPLDIQATATSNGIDVDIRGSGPLPPPRIAALSLVAEKHRLARLTRHGELVLMRNPPVMTMGTAQV
TLPPGSFLQATAQGEETLAQLVLDRVGKAKQVADLFCGVGPFALRLASRGRVIAMDTDAGSIAALQKAAGTPSLKPIKAE
ARDLFRRPLMPPELRDIDAVVFDPPRQGAQAQAKQLAASKVPVIVAVSCNVTTFARDVRMLIDGGYKLETVVPVDQFRHT
PHVELVARLVR
>Mature_411_residues
MVERLVIDHVGHRGDGVSLSSGEALYVPYTLAGETVEAAAIPGHPDRRKLLAVEAPSPERITPFCPHFGVCGGCAIQHWS
PEPYRQWKRNIVVETLRAAKVDCEVAPLIDAHGAGRRRATFHARMGTHDILRVGFSAANSHEIVPIDRCPILDPALDGAL
EAAWALAEALKPTGKPLDIQATATSNGIDVDIRGSGPLPPPRIAALSLVAEKHRLARLTRHGELVLMRNPPVMTMGTAQV
TLPPGSFLQATAQGEETLAQLVLDRVGKAKQVADLFCGVGPFALRLASRGRVIAMDTDAGSIAALQKAAGTPSLKPIKAE
ARDLFRRPLMPPELRDIDAVVFDPPRQGAQAQAKQLAASKVPVIVAVSCNVTTFARDVRMLIDGGYKLETVVPVDQFRHT
PHVELVARLVR

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789148, Length=428, Percent_Identity=28.2710280373832, Blast_Score=138, Evalue=5e-34,
Organism=Escherichia coli, GI1787083, Length=172, Percent_Identity=29.0697674418605, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR007848
- InterPro:   IPR010280 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: NA

Molecular weight: Translated: 44155; Mature: 44155

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVERLVIDHVGHRGDGVSLSSGEALYVPYTLAGETVEAAAIPGHPDRRKLLAVEAPSPER
CCCCEEHHHCCCCCCCCEECCCCEEEEEEEECCCCEEEEECCCCCCCCEEEEEECCCCCC
ITPFCPHFGVCGGCAIQHWSPEPYRQWKRNIVVETLRAAKVDCEVAPLIDAHGAGRRRAT
CCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEECEECCCCCCCCCEE
FHARMGTHDILRVGFSAANSHEIVPIDRCPILDPALDGALEAAWALAEALKPTGKPLDIQ
EEECCCCHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEE
ATATSNGIDVDIRGSGPLPPPRIAALSLVAEKHRLARLTRHGELVLMRNPPVMTMGTAQV
EECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEECCEEE
TLPPGSFLQATAQGEETLAQLVLDRVGKAKQVADLFCGVGPFALRLASRGRVIAMDTDAG
EECCCHHHEECCCCHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHCCCCEEEEECCCC
SIAALQKAAGTPSLKPIKAEARDLFRRPLMPPELRDIDAVVFDPPRQGAQAQAKQLAASK
HHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHCC
VPVIVAVSCNVTTFARDVRMLIDGGYKLETVVPVDQFRHTPHVELVARLVR
CCEEEEEECCHHHHHHHHHHHHCCCEEEEEECCHHHHCCCCHHHHHHHHHC
>Mature Secondary Structure
MVERLVIDHVGHRGDGVSLSSGEALYVPYTLAGETVEAAAIPGHPDRRKLLAVEAPSPER
CCCCEEHHHCCCCCCCCEECCCCEEEEEEEECCCCEEEEECCCCCCCCEEEEEECCCCCC
ITPFCPHFGVCGGCAIQHWSPEPYRQWKRNIVVETLRAAKVDCEVAPLIDAHGAGRRRAT
CCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEECEECCCCCCCCCEE
FHARMGTHDILRVGFSAANSHEIVPIDRCPILDPALDGALEAAWALAEALKPTGKPLDIQ
EEECCCCHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEE
ATATSNGIDVDIRGSGPLPPPRIAALSLVAEKHRLARLTRHGELVLMRNPPVMTMGTAQV
EECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEECCEEE
TLPPGSFLQATAQGEETLAQLVLDRVGKAKQVADLFCGVGPFALRLASRGRVIAMDTDAG
EECCCHHHEECCCCHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHCCCCEEEEECCCC
SIAALQKAAGTPSLKPIKAEARDLFRRPLMPPELRDIDAVVFDPPRQGAQAQAKQLAASK
HHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHCC
VPVIVAVSCNVTTFARDVRMLIDGGYKLETVVPVDQFRHTPHVELVARLVR
CCEEEEEECCHHHHHHHHHHHHCCCEEEEEECCHHHHCCCCHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]