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The map label for this gene is fabI [H]

Identifier: 146339191

GI number: 146339191

Start: 2286872

End: 2287705

Strand: Direct

Name: fabI [H]

Synonym: BRADO2151

Alternate gene names: 146339191

Gene position: 2286872-2287705 (Clockwise)

Preceding gene: 146339188

Following gene: 146339192

Centisome position: 30.67

GC content: 64.27

Gene sequence:

>834_bases
ATGGCGCAGATTTCAGGTTTGATGCAGGGCAAGCGCGGGGTCATTCTCGGCGTCGCCAACAACCGCTCGATCGCCTGGGG
CATCGCCAAGGCCGCCCGCGCCGCCGGCGCCGAGATCGCGCTGACCTATCAGGGCGACGCGCTGAAGAAGCGGGTCGAGC
CGCTGGCCGCCGAGCTCGGCGGCCTCGTGCTCGGGCATTGCGACGTGACCGATCCCGCAACCATCGACGCCATCTTCGAC
GTGCTCAAGGAGAAATGGGGCAAGATCGACTTCGTCGTTCACGCCATCGCCTTCTCTGACAAGGACCAGCTCGACGGCCG
CTATATCGAGACCACGCAGGACAATTTCTCCAAGAGCATGCTGATCAGCTGCTACTCGCTGACCGCGGTCGCGCAGCGCG
CGGAGAAGCTGATGACCGATGGTGGCTCGATCATCACGCTGACCTATTACGGCGCCGAGAAGTGGATGCCCCATTATAAC
GTCATGGGTGTCGCGAAAGCCGCGCTGGAGGCCAGCGTGCGCTACCTCGCTGCCGACCTCGGCGAGAAGAACATCCGCGT
CAACGCCATCTCGGCCGGTCCGATCAAGACGCTGGCCGCCTCAGGCATCGGCGATTTCCGCTATATCCTGAAGTGGAACG
AGTACAACGCGCCGCTGCGCCGGACCGTGACGATCGAGGAGGTCGGTGACAGCGCCCTTTACCTGCTCTCCGACCTGTCG
CGCGGCGTGACCGGCGAGGTGCACCACGTCGATTCCGGCTATCATGTCGTCGGCATGAAGCGGCCGGAGGCACCGGACAT
CTCGCTGAGCAACTCGGCCGGCGCCAAGGACTAG

Upstream 100 bases:

>100_bases
TCTTGCCGGGAGTTTGCCACAGAGCGGGCGGAATGGGGCTGTACCGGGCCAGACCGGTGGTGTACGTCCAATCGTTAATA
ATCGCTCTATGGGAAGCCGC

Downstream 100 bases:

>100_bases
TTCATCGCGCGCATGCCCCGGCCCACGATCTACTACATCCGCCACGGCGAGACGGAATGGAATGCTCTCGGCCGGCTGCA
GGGCACGCAGGACATTCCGC

Product: enoyl-(acyl carrier protein) reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase 1 [H]

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MAQISGLMQGKRGVILGVANNRSIAWGIAKAARAAGAEIALTYQGDALKKRVEPLAAELGGLVLGHCDVTDPATIDAIFD
VLKEKWGKIDFVVHAIAFSDKDQLDGRYIETTQDNFSKSMLISCYSLTAVAQRAEKLMTDGGSIITLTYYGAEKWMPHYN
VMGVAKAALEASVRYLAADLGEKNIRVNAISAGPIKTLAASGIGDFRYILKWNEYNAPLRRTVTIEEVGDSALYLLSDLS
RGVTGEVHHVDSGYHVVGMKRPEAPDISLSNSAGAKD

Sequences:

>Translated_277_residues
MAQISGLMQGKRGVILGVANNRSIAWGIAKAARAAGAEIALTYQGDALKKRVEPLAAELGGLVLGHCDVTDPATIDAIFD
VLKEKWGKIDFVVHAIAFSDKDQLDGRYIETTQDNFSKSMLISCYSLTAVAQRAEKLMTDGGSIITLTYYGAEKWMPHYN
VMGVAKAALEASVRYLAADLGEKNIRVNAISAGPIKTLAASGIGDFRYILKWNEYNAPLRRTVTIEEVGDSALYLLSDLS
RGVTGEVHHVDSGYHVVGMKRPEAPDISLSNSAGAKD
>Mature_276_residues
AQISGLMQGKRGVILGVANNRSIAWGIAKAARAAGAEIALTYQGDALKKRVEPLAAELGGLVLGHCDVTDPATIDAIFDV
LKEKWGKIDFVVHAIAFSDKDQLDGRYIETTQDNFSKSMLISCYSLTAVAQRAEKLMTDGGSIITLTYYGAEKWMPHYNV
MGVAKAALEASVRYLAADLGEKNIRVNAISAGPIKTLAASGIGDFRYILKWNEYNAPLRRTVTIEEVGDSALYLLSDLSR
GVTGEVHHVDSGYHVVGMKRPEAPDISLSNSAGAKD

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787545, Length=259, Percent_Identity=51.3513513513513, Blast_Score=265, Evalue=2e-72,
Organism=Escherichia coli, GI1787905, Length=250, Percent_Identity=28.4, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1789378, Length=146, Percent_Identity=34.9315068493151, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI2367365, Length=253, Percent_Identity=28.4584980237154, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI87082100, Length=263, Percent_Identity=28.5171102661597, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787335, Length=259, Percent_Identity=25.0965250965251, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 29804; Mature: 29673

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQISGLMQGKRGVILGVANNRSIAWGIAKAARAAGAEIALTYQGDALKKRVEPLAAELG
CCCCCHHHCCCCEEEEEEECCCEEEHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHC
GLVLGHCDVTDPATIDAIFDVLKEKWGKIDFVVHAIAFSDKDQLDGRYIETTQDNFSKSM
CEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCCCEEEECCCCCHHHHH
LISCYSLTAVAQRAEKLMTDGGSIITLTYYGAEKWMPHYNVMGVAKAALEASVRYLAADL
HHHHHHHHHHHHHHHHHHHCCCCEEEEEEECHHHCCCCCCHHHHHHHHHHHHHHHHHHHC
GEKNIRVNAISAGPIKTLAASGIGDFRYILKWNEYNAPLRRTVTIEEVGDSALYLLSDLS
CCCCEEEEEEECCCHHHHHHCCCCCEEEEEEECCCCCCEEEEEEHHHHCCHHHHHHHHHC
RGVTGEVHHVDSGYHVVGMKRPEAPDISLSNSAGAKD
CCCCCEEEEECCCEEEEEECCCCCCCCEECCCCCCCC
>Mature Secondary Structure 
AQISGLMQGKRGVILGVANNRSIAWGIAKAARAAGAEIALTYQGDALKKRVEPLAAELG
CCCCHHHCCCCEEEEEEECCCEEEHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHC
GLVLGHCDVTDPATIDAIFDVLKEKWGKIDFVVHAIAFSDKDQLDGRYIETTQDNFSKSM
CEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCCCEEEECCCCCHHHHH
LISCYSLTAVAQRAEKLMTDGGSIITLTYYGAEKWMPHYNVMGVAKAALEASVRYLAADL
HHHHHHHHHHHHHHHHHHHCCCCEEEEEEECHHHCCCCCCHHHHHHHHHHHHHHHHHHHC
GEKNIRVNAISAGPIKTLAASGIGDFRYILKWNEYNAPLRRTVTIEEVGDSALYLLSDLS
CCCCEEEEEEECCCHHHHHHCCCCCEEEEEEECCCCCCEEEEEEHHHHCCHHHHHHHHHC
RGVTGEVHHVDSGYHVVGMKRPEAPDISLSNSAGAKD
CCCCCEEEEECCCEEEEEECCCCCCCCEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]