BacMap

Definition	Bradyrhizobium sp. ORS278 chromosome, complete genome.
Accession	NC_009445
Length	7,456,587

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The map label for this gene is cbpA [H]

Identifier: 146339188

GI number: 146339188

Start: 2283842

End: 2284804

Strand: Direct

Name: cbpA [H]

Synonym: BRADO2148

Alternate gene names: 146339188

Gene position: 2283842-2284804 (Clockwise)

Preceding gene: 146339187

Following gene: 146339191

Centisome position: 30.63

GC content: 67.29

Gene sequence:

>963_bases
ATGCGCGACCCCTATGAGGTCTTGGGGGTGCCGCGAAGCGCCAGCGCTGCGGCCATCAAGAGCGCCTATCGCAAGCTCGC
CAAGAAGCATCATCCCGACAGCAACAAGGATGACCCGAAGGCATCGGCCAGGTTTGCCGAGATCAACTCGGCCAACGAGA
TCCTGGGCGACGAGGAGAAGCGGAAGCAGTTCGATCGCGGGGAGATCGATGCCGACGGCAAGCCCCGCTTCCAGGGCTTT
CCTGGCGGCGGCGCCGGCCGTGGCGGTCCCGGCGGGTTCGAGAATTACACCTTCCGCAGCGGCGGCGGACCGGGCGGCGG
CTTCGGCGGCGGTGGCGGCGGCTTCGAGGATATTCTCAACAGCATGTTCGGCGGCGCGGGGCGTGGCGGACGCGGCGGCG
GCAGCCCGTTCGAGTTCGACACCGGCGGTGTCGGCATCGATCTCGACCTCAAGGTCGCGATGTCGGTGACGCTCGAGGAG
TCGGTCAAGGGTGGCGAAAAGCGCGTGCGCCTGCCCTCGGGCAAGGAGCTCAACGTCAAGGTCCCGCCCGGCGTCACCGC
CGGCCAGCAGATCCGGCTGAAAGGGCAGGGCGAGAGCGCGCCCGGTCATCGGCCCGGCGACCTCCTGATCACCATCCAGA
TCGCGCCGCATCCGTTCTTCAAGGTCGAGGGCAGCGACGTCAGGCTCGATCTGCCCATCACGCTGTACGAGGCGGTGCTG
GGCGCCAAGGTGCGCGCCCCGACCCTCGGCAGCGCGGTCGAATTGTCGATCCCGAAGAACACCTCCAGCGGGCGGACTTT
CCGGCTGAAGGGCAAGGGCCTGCCCAAAAGCGACGGCTCGGCGGGCGATCTGTTCATCACCACGAAGATCGTGCTGCCCG
ACGGCAACGATGCCGAGCTCGAGGCGCTGATGACCAAGTGGCGCGACAAGCACCCCTACAATCCGCGCAGCAATCTGTCG
TAA

Upstream 100 bases:

>100_bases
GGAACCCGTTGCAAAAATACAACGGACTCTCCAGATGAAATGCAAAGCGGGCGGGGAGCCCTTAGGTTAATATCTGACTT
TCTGTAAGGAGACGTGACGG

Downstream 100 bases:

>100_bases
CGAAAGATCACGCTGGCTGTTAGCTCCGTCTTGCCCGGGCTTGTCCCGGGCATTCACGCCTATCCCAGGGTGCGGGCCAA
CGTGGATGGCCGGGACAAGC

Product: putative heat shock protein DnaJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MRDPYEVLGVPRSASAAAIKSAYRKLAKKHHPDSNKDDPKASARFAEINSANEILGDEEKRKQFDRGEIDADGKPRFQGF
PGGGAGRGGPGGFENYTFRSGGGPGGGFGGGGGGFEDILNSMFGGAGRGGRGGGSPFEFDTGGVGIDLDLKVAMSVTLEE
SVKGGEKRVRLPSGKELNVKVPPGVTAGQQIRLKGQGESAPGHRPGDLLITIQIAPHPFFKVEGSDVRLDLPITLYEAVL
GAKVRAPTLGSAVELSIPKNTSSGRTFRLKGKGLPKSDGSAGDLFITTKIVLPDGNDAELEALMTKWRDKHPYNPRSNLS

Sequences:

>Translated_320_residues
MRDPYEVLGVPRSASAAAIKSAYRKLAKKHHPDSNKDDPKASARFAEINSANEILGDEEKRKQFDRGEIDADGKPRFQGF
PGGGAGRGGPGGFENYTFRSGGGPGGGFGGGGGGFEDILNSMFGGAGRGGRGGGSPFEFDTGGVGIDLDLKVAMSVTLEE
SVKGGEKRVRLPSGKELNVKVPPGVTAGQQIRLKGQGESAPGHRPGDLLITIQIAPHPFFKVEGSDVRLDLPITLYEAVL
GAKVRAPTLGSAVELSIPKNTSSGRTFRLKGKGLPKSDGSAGDLFITTKIVLPDGNDAELEALMTKWRDKHPYNPRSNLS
>Mature_320_residues
MRDPYEVLGVPRSASAAAIKSAYRKLAKKHHPDSNKDDPKASARFAEINSANEILGDEEKRKQFDRGEIDADGKPRFQGF
PGGGAGRGGPGGFENYTFRSGGGPGGGFGGGGGGFEDILNSMFGGAGRGGRGGGSPFEFDTGGVGIDLDLKVAMSVTLEE
SVKGGEKRVRLPSGKELNVKVPPGVTAGQQIRLKGQGESAPGHRPGDLLITIQIAPHPFFKVEGSDVRLDLPITLYEAVL
GAKVRAPTLGSAVELSIPKNTSSGRTFRLKGKGLPKSDGSAGDLFITTKIVLPDGNDAELEALMTKWRDKHPYNPRSNLS

Specific function: DNA-binding protein that preferentially recognizes a curved DNA sequence. It is probably a functional analog of DnaJ; displays overlapping activities with DnaJ, but functions under different conditions, probably acting as a molecular chaperone in an adapt

COG id: COG2214

COG function: function code O; DnaJ-class molecular chaperone

Gene ontology:

Cell location: Cytoplasm, nucleoid [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 J domain [H]

Homologues:

Organism=Homo sapiens, GI205360840, Length=345, Percent_Identity=31.0144927536232, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI205360838, Length=345, Percent_Identity=31.0144927536232, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI6631085, Length=344, Percent_Identity=31.1046511627907, Blast_Score=111, Evalue=7e-25,
Organism=Homo sapiens, GI201862587, Length=341, Percent_Identity=30.4985337243402, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI201862518, Length=341, Percent_Identity=30.4985337243402, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI56549115, Length=341, Percent_Identity=30.4985337243402, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI5453690, Length=335, Percent_Identity=29.5522388059701, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI39204547, Length=308, Percent_Identity=28.2467532467532, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI4504511, Length=327, Percent_Identity=26.605504587156, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI7706495, Length=64, Percent_Identity=59.375, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI56687498, Length=64, Percent_Identity=57.8125, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI29126218, Length=62, Percent_Identity=54.8387096774194, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI88501736, Length=152, Percent_Identity=38.1578947368421, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI47777312, Length=149, Percent_Identity=38.255033557047, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI9558755, Length=206, Percent_Identity=31.5533980582524, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI27151736, Length=152, Percent_Identity=38.1578947368421, Blast_Score=72, Evalue=9e-13,
Organism=Homo sapiens, GI45504382, Length=62, Percent_Identity=50, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI23503241, Length=127, Percent_Identity=38.5826771653543, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI24308127, Length=65, Percent_Identity=46.1538461538462, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI5031741, Length=62, Percent_Identity=51.6129032258064, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI1787235, Length=332, Percent_Identity=34.9397590361446, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1786197, Length=142, Percent_Identity=35.9154929577465, Blast_Score=99, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17534355, Length=345, Percent_Identity=31.5942028985507, Blast_Score=124, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI212645553, Length=65, Percent_Identity=53.8461538461538, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI115532378, Length=64, Percent_Identity=51.5625, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI115532476, Length=64, Percent_Identity=53.125, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17554644, Length=90, Percent_Identity=43.3333333333333, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17551190, Length=69, Percent_Identity=53.6231884057971, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17554900, Length=75, Percent_Identity=45.3333333333333, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17553096, Length=63, Percent_Identity=49.2063492063492, Blast_Score=66, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17553098, Length=63, Percent_Identity=49.2063492063492, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6324265, Length=345, Percent_Identity=28.695652173913, Blast_Score=86, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6323813, Length=140, Percent_Identity=42.8571428571429, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323119, Length=92, Percent_Identity=44.5652173913043, Blast_Score=67, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6322388, Length=117, Percent_Identity=36.7521367521368, Blast_Score=67, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6322194, Length=62, Percent_Identity=45.1612903225806, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323870, Length=70, Percent_Identity=41.4285714285714, Blast_Score=63, Evalue=5e-11,
Organism=Drosophila melanogaster, GI85726398, Length=344, Percent_Identity=30.2325581395349, Blast_Score=131, Evalue=7e-31,
Organism=Drosophila melanogaster, GI24658562, Length=329, Percent_Identity=35.8662613981763, Blast_Score=130, Evalue=9e-31,
Organism=Drosophila melanogaster, GI24658555, Length=329, Percent_Identity=35.8662613981763, Blast_Score=130, Evalue=9e-31,
Organism=Drosophila melanogaster, GI19920464, Length=328, Percent_Identity=28.3536585365854, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI45549272, Length=365, Percent_Identity=26.5753424657534, Blast_Score=110, Evalue=9e-25,
Organism=Drosophila melanogaster, GI45552813, Length=365, Percent_Identity=26.5753424657534, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI45552811, Length=365, Percent_Identity=26.5753424657534, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI18858081, Length=350, Percent_Identity=28, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24646562, Length=343, Percent_Identity=25.9475218658892, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24646560, Length=343, Percent_Identity=25.9475218658892, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24646558, Length=343, Percent_Identity=25.9475218658892, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24646556, Length=343, Percent_Identity=25.9475218658892, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI21357547, Length=343, Percent_Identity=25.9475218658892, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI21355911, Length=99, Percent_Identity=46.4646464646465, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI17737735, Length=62, Percent_Identity=48.3870967741936, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28574723, Length=62, Percent_Identity=48.3870967741936, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24668523, Length=62, Percent_Identity=48.3870967741936, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24654066, Length=66, Percent_Identity=50, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24654070, Length=66, Percent_Identity=50, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24654068, Length=66, Percent_Identity=50, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24654072, Length=66, Percent_Identity=50, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI161077138, Length=66, Percent_Identity=48.4848484848485, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002939
- InterPro:   IPR001623
- InterPro:   IPR018253
- InterPro:   IPR008971
- InterPro:   IPR015609
- InterPro:   IPR003095 [H]

Pfam domain/function: PF00226 DnaJ; PF01556 DnaJ_C [H]

EC number: NA

Molecular weight: Translated: 33608; Mature: 33608

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: PS00636 DNAJ_1 ; PS50076 DNAJ_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDPYEVLGVPRSASAAAIKSAYRKLAKKHHPDSNKDDPKASARFAEINSANEILGDEEK
CCCCHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHEEHHCCCHHHHCCCHHH
RKQFDRGEIDADGKPRFQGFPGGGAGRGGPGGFENYTFRSGGGPGGGFGGGGGGFEDILN
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHH
SMFGGAGRGGRGGGSPFEFDTGGVGIDLDLKVAMSVTLEESVKGGEKRVRLPSGKELNVK
HHHCCCCCCCCCCCCCCEECCCCEEEEEEEEEEEEEEEHHHHCCCCEEEECCCCCEEEEE
VPPGVTAGQQIRLKGQGESAPGHRPGDLLITIQIAPHPFFKVEGSDVRLDLPITLYEAVL
CCCCCCCCCEEEEECCCCCCCCCCCCCEEEEEEECCCCEEEECCCCEEEECCHHHHHHHH
GAKVRAPTLGSAVELSIPKNTSSGRTFRLKGKGLPKSDGSAGDLFITTKIVLPDGNDAEL
CCCCCCCCCCCEEEEEECCCCCCCCEEEEECCCCCCCCCCCCEEEEEEEEEECCCCCHHH
EALMTKWRDKHPYNPRSNLS
HHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MRDPYEVLGVPRSASAAAIKSAYRKLAKKHHPDSNKDDPKASARFAEINSANEILGDEEK
CCCCHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHEEHHCCCHHHHCCCHHH
RKQFDRGEIDADGKPRFQGFPGGGAGRGGPGGFENYTFRSGGGPGGGFGGGGGGFEDILN
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHH
SMFGGAGRGGRGGGSPFEFDTGGVGIDLDLKVAMSVTLEESVKGGEKRVRLPSGKELNVK
HHHCCCCCCCCCCCCCCEECCCCEEEEEEEEEEEEEEEHHHHCCCCEEEECCCCCEEEEE
VPPGVTAGQQIRLKGQGESAPGHRPGDLLITIQIAPHPFFKVEGSDVRLDLPITLYEAVL
CCCCCCCCCEEEEECCCCCCCCCCCCCEEEEEEECCCCEEEECCCCEEEECCHHHHHHHH
GAKVRAPTLGSAVELSIPKNTSSGRTFRLKGKGLPKSDGSAGDLFITTKIVLPDGNDAEL
CCCCCCCCCCCEEEEEECCCCCCCCEEEEECCCCCCCCCCCCEEEEEEEEEECCCCCHHH
EALMTKWRDKHPYNPRSNLS
HHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA