BacMap

Definition	Bradyrhizobium sp. ORS278 chromosome, complete genome.
Accession	NC_009445
Length	7,456,587

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The map label for this gene is fixR1 [H]

Identifier: 146339185

GI number: 146339185

Start: 2281658

End: 2282377

Strand: Reverse

Name: fixR1 [H]

Synonym: BRADO2145

Alternate gene names: 146339185

Gene position: 2282377-2281658 (Counterclockwise)

Preceding gene: 146339186

Following gene: 146339182

Centisome position: 30.61

GC content: 67.64

Gene sequence:

>720_bases
ATGCTGCTGACCGGCGCCAGTCGCGGCATCGGTCATGCCACCGTGATCCGATTCTCCTCCGCCGGCTGGCGCGTCATCAC
CTGCTCGCGGCATCCCTTCCCGGAGGAGTGCCCGTGGGGCGCCGGACCCGAGGACCACATCCAGGTCGACCTCGCCGACC
CCCAGGATACGTTGCGCGCGATCGAGGAGATCAAGCAGCGCATGGACAGCGGCGAGCTGCATGCGCTCGTCAACAACGCC
GCGATCTCGCCGAAGGGCCAGGGCGGCGCGCGGCTCGGGACCGTGGATACCGATCTCGACACCTGGAATCAGGTCTTCCG
CGTCAACTTCTTCGCGCCGATCATGATGGCGCGCGGCCTGATCAAGGAGTTGAAGGCGGCCAAGGGCTCGGTGGTGAACG
TCACCTCGATCGCCGGCTCGCGCGTCCATCCGTTCGCCGGCGCGGCCTATGCCACCTCCAAGGCGGCGCTCGCCGCGCTC
ACGCGCGAGATGGCGTCGGACTTCGGCCGTGTCGGTGTGCGCGTCAACTCGATCGCGCCGGGCGAGATCGACACCTCGAT
CCTGTCGCCGGGCACCGAGAAGATCGTGCAGGAGCAGATCCCGATGCAGCGGCTCGGCACGCCCGACGAGGTCGCCAAGA
TCATCTACGTGCTGTGCACCGAGACGAGCTCCTACGTGAACGGCGCCGAGATCCACATCAACGGCGGCCAGCACGTGTGA

Upstream 100 bases:

>100_bases
AACGCGCACCGAACATGCAACCCTTGTGGTAAGCTGATCCCTCGTGGATCGGGAGAGATATCTCATCATGCCGACCTCCT
CCAACCAGCCGCGGCGGACG

Downstream 100 bases:

>100_bases
GGACGATCGGTCGCGGCCACGAGGACACGATGCGGTGTCTGCGGCTTGGTTCACACCAGCTTGCGCCGCTCAACGAACCA
CGGCTCGCCACACCAAGATG

Product: short-chain dehydrogenase

Products: (3R)-hydroxyacyl-[acyl-carrier-protein]; NADP; NADPH; Proton; beta-ketoacyl-ACP [C]

Alternate protein names: NA

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MLLTGASRGIGHATVIRFSSAGWRVITCSRHPFPEECPWGAGPEDHIQVDLADPQDTLRAIEEIKQRMDSGELHALVNNA
AISPKGQGGARLGTVDTDLDTWNQVFRVNFFAPIMMARGLIKELKAAKGSVVNVTSIAGSRVHPFAGAAYATSKAALAAL
TREMASDFGRVGVRVNSIAPGEIDTSILSPGTEKIVQEQIPMQRLGTPDEVAKIIYVLCTETSSYVNGAEIHINGGQHV

Sequences:

>Translated_239_residues
MLLTGASRGIGHATVIRFSSAGWRVITCSRHPFPEECPWGAGPEDHIQVDLADPQDTLRAIEEIKQRMDSGELHALVNNA
AISPKGQGGARLGTVDTDLDTWNQVFRVNFFAPIMMARGLIKELKAAKGSVVNVTSIAGSRVHPFAGAAYATSKAALAAL
TREMASDFGRVGVRVNSIAPGEIDTSILSPGTEKIVQEQIPMQRLGTPDEVAKIIYVLCTETSSYVNGAEIHINGGQHV
>Mature_239_residues
MLLTGASRGIGHATVIRFSSAGWRVITCSRHPFPEECPWGAGPEDHIQVDLADPQDTLRAIEEIKQRMDSGELHALVNNA
AISPKGQGGARLGTVDTDLDTWNQVFRVNFFAPIMMARGLIKELKAAKGSVVNVTSIAGSRVHPFAGAAYATSKAALAAL
TREMASDFGRVGVRVNSIAPGEIDTSILSPGTEKIVQEQIPMQRLGTPDEVAKIIYVLCTETSSYVNGAEIHINGGQHV

Specific function: Fatty acid biosynthesis pathway; first reduction step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI15277342, Length=257, Percent_Identity=29.9610894941634, Blast_Score=95, Evalue=6e-20,
Organism=Homo sapiens, GI32483357, Length=247, Percent_Identity=29.1497975708502, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI40254992, Length=235, Percent_Identity=27.2340425531915, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI7705925, Length=250, Percent_Identity=30, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI5031737, Length=249, Percent_Identity=29.718875502008, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI4503301, Length=202, Percent_Identity=32.1782178217822, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI59889578, Length=236, Percent_Identity=25.8474576271186, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI119392083, Length=192, Percent_Identity=32.2916666666667, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI126723750, Length=174, Percent_Identity=28.735632183908, Blast_Score=74, Evalue=8e-14,
Organism=Homo sapiens, GI66933014, Length=155, Percent_Identity=29.6774193548387, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI20149619, Length=199, Percent_Identity=28.643216080402, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI19923817, Length=262, Percent_Identity=26.7175572519084, Blast_Score=71, Evalue=8e-13,
Organism=Homo sapiens, GI4504503, Length=195, Percent_Identity=27.6923076923077, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI40548397, Length=211, Percent_Identity=24.1706161137441, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI214010158, Length=211, Percent_Identity=24.1706161137441, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI214010156, Length=211, Percent_Identity=24.1706161137441, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI22758144, Length=211, Percent_Identity=24.1706161137441, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI109715829, Length=237, Percent_Identity=27.4261603375527, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1787335, Length=244, Percent_Identity=26.6393442622951, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI87082100, Length=257, Percent_Identity=29.1828793774319, Blast_Score=91, Evalue=5e-20,
Organism=Escherichia coli, GI2367175, Length=250, Percent_Identity=30, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1788459, Length=201, Percent_Identity=30.8457711442786, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI2367365, Length=239, Percent_Identity=30.1255230125523, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1786812, Length=258, Percent_Identity=27.5193798449612, Blast_Score=80, Evalue=8e-17,
Organism=Escherichia coli, GI1787905, Length=246, Percent_Identity=30.0813008130081, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1789378, Length=264, Percent_Identity=29.5454545454545, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1790717, Length=254, Percent_Identity=26.3779527559055, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI87082160, Length=251, Percent_Identity=26.6932270916335, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1789208, Length=199, Percent_Identity=27.1356783919598, Blast_Score=64, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17562906, Length=257, Percent_Identity=34.2412451361868, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17560676, Length=248, Percent_Identity=30.6451612903226, Blast_Score=100, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI17555706, Length=243, Percent_Identity=28.3950617283951, Blast_Score=100, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI17531453, Length=254, Percent_Identity=33.4645669291339, Blast_Score=99, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17563726, Length=261, Percent_Identity=32.183908045977, Blast_Score=97, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17562908, Length=257, Percent_Identity=32.295719844358, Blast_Score=97, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI72000259, Length=260, Percent_Identity=32.6923076923077, Blast_Score=97, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17560150, Length=263, Percent_Identity=31.5589353612167, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17562990, Length=260, Percent_Identity=33.8461538461538, Blast_Score=94, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17560332, Length=258, Percent_Identity=32.5581395348837, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI193204405, Length=259, Percent_Identity=31.6602316602317, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17562910, Length=257, Percent_Identity=29.1828793774319, Blast_Score=90, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17559104, Length=260, Percent_Identity=30, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17562904, Length=263, Percent_Identity=31.5589353612167, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17544670, Length=265, Percent_Identity=29.0566037735849, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71994600, Length=257, Percent_Identity=28.4046692607004, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17561402, Length=249, Percent_Identity=28.5140562248996, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI115534694, Length=247, Percent_Identity=28.3400809716599, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17560220, Length=257, Percent_Identity=29.5719844357977, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17538480, Length=260, Percent_Identity=28.8461538461538, Blast_Score=81, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI71994604, Length=256, Percent_Identity=26.171875, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI25147288, Length=242, Percent_Identity=25.6198347107438, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17565030, Length=266, Percent_Identity=28.9473684210526, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17551412, Length=256, Percent_Identity=29.296875, Blast_Score=74, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17538486, Length=265, Percent_Identity=26.7924528301887, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17561272, Length=209, Percent_Identity=27.7511961722488, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17538182, Length=254, Percent_Identity=26.7716535433071, Blast_Score=67, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17536651, Length=257, Percent_Identity=26.4591439688716, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17536025, Length=260, Percent_Identity=26.1538461538462, Blast_Score=64, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6324126, Length=142, Percent_Identity=32.3943661971831, Blast_Score=71, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6322067, Length=189, Percent_Identity=29.1005291005291, Blast_Score=69, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6323882, Length=199, Percent_Identity=29.6482412060301, Blast_Score=65, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24639444, Length=244, Percent_Identity=31.5573770491803, Blast_Score=107, Evalue=7e-24,
Organism=Drosophila melanogaster, GI23397609, Length=249, Percent_Identity=32.9317269076305, Blast_Score=106, Evalue=1e-23,
Organism=Drosophila melanogaster, GI21355319, Length=246, Percent_Identity=31.3008130081301, Blast_Score=104, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24644339, Length=254, Percent_Identity=31.496062992126, Blast_Score=93, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24644337, Length=211, Percent_Identity=33.175355450237, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24643142, Length=251, Percent_Identity=33.4661354581673, Blast_Score=88, Evalue=6e-18,
Organism=Drosophila melanogaster, GI21357041, Length=254, Percent_Identity=27.9527559055118, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI28571526, Length=254, Percent_Identity=27.5590551181102, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI17737361, Length=258, Percent_Identity=28.2945736434109, Blast_Score=71, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.100 [C]

Molecular weight: Translated: 25542; Mature: 25542

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLTGASRGIGHATVIRFSSAGWRVITCSRHPFPEECPWGAGPEDHIQVDLADPQDTLRA
CEECCCCCCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEECCCHHHHHH
IEEIKQRMDSGELHALVNNAAISPKGQGGARLGTVDTDLDTWNQVFRVNFFAPIMMARGL
HHHHHHHCCCCCEEEEECCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
IKELKAAKGSVVNVTSIAGSRVHPFAGAAYATSKAALAALTREMASDFGRVGVRVNSIAP
HHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCC
GEIDTSILSPGTEKIVQEQIPMQRLGTPDEVAKIIYVLCTETSSYVNGAEIHINGGQHV
CCCCHHHCCCCHHHHHHHHCCHHHCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCC
>Mature Secondary Structure
MLLTGASRGIGHATVIRFSSAGWRVITCSRHPFPEECPWGAGPEDHIQVDLADPQDTLRA
CEECCCCCCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEECCCHHHHHH
IEEIKQRMDSGELHALVNNAAISPKGQGGARLGTVDTDLDTWNQVFRVNFFAPIMMARGL
HHHHHHHCCCCCEEEEECCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
IKELKAAKGSVVNVTSIAGSRVHPFAGAAYATSKAALAALTREMASDFGRVGVRVNSIAP
HHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCC
GEIDTSILSPGTEKIVQEQIPMQRLGTPDEVAKIIYVLCTETSSYVNGAEIHINGGQHV
CCCCHHHCCCCHHHHHHHHCCHHHCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Oxoacyl-[acyl-carrier-protein]; NADPH; D-3-hydroxy-acyl-ACP; NADP [C]

Specific reaction: Oxoacyl-[acyl-carrier-protein] + NADPH = (3R)-hydroxyacyl-[acyl-carrier-protein] + NADP+ D-3-hydroxy-acyl-ACP + NADP = NADPH + Proton + beta-ketoacyl-ACP [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3313281; 11157954; 12597275 [H]