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The map label for this gene is smeIM [H]

Identifier: 146339056

GI number: 146339056

Start: 2136827

End: 2137861

Strand: Reverse

Name: smeIM [H]

Synonym: BRADO2010

Alternate gene names: 146339056

Gene position: 2137861-2136827 (Counterclockwise)

Preceding gene: 146339059

Following gene: 146339050

Centisome position: 28.67

GC content: 66.38

Gene sequence:

>1035_bases
ATGTCGAAACTGCCAGGCGCATCCGTCGACCTGGTGTTCGCTGATCCGCCCTACAATCTGCAGCTCAAGGGTGAGCTCAA
ACGTCCCGACGAATCGCAGGTCGATGCCGTCGACGACGATTGGGACAAGTTCTCCTCGTTCACGGCCTACGACGATTTCA
CGCGCGCCTGGCTGCTCGCCGCGCGCCGCGTGATGAAGCCGAGCGCGACGATCTGGGTGATCGGCTCCTATCACAACATC
TTCCGCGTCGGCGCGATCATGCAGGATCTCGGCTTCTGGATCCTCAACGACATCGTCTGGCGCAAGTCGAACCCGATGCC
CAACTTCCGCGGCCGCCGCTTCACCAACGCGCACGAGACCATGATCTGGGCCGCGCGGGACGAGAAGGCGAAGGGCTACA
CCTTCAACTACGAAGCGCTGAAGGCGTCGAACGAGGACGTGCAGGCGCGCTCGGACTGGCTGATCCCGCTGTGCACCGGC
GAGGAGCGGCTGAAGGACGGCGACGGCAAGAAGGTTCATCCGACGCAAAAGCCGGAAGGCCTGCTCGCGCGCGTGCTGCT
GTCATCGTCGAAGCAGGGCGACCTGGTGATCGATCCTTTCAACGGCACCGGCACCACCGGCGCCGTGGCCAAGCGCCTCG
GCCGCCGCTACATCGGCTTCGAGCGCGACAAGGCCTATGCGGAAGCCGCCGAGCGGCGTATTGCCGCGGTCGAGCCGCTC
CCCGCGGAGACGCTCGCGCCGTTCATGACGGCGCGCGAGGCGCCGCGGGTGGCCTTCTCAGAACTGATCGAGCGCGGCAT
GATCGCGCCAGGCGCCAAGCTTGTCGACTCCAAGCGCCGCTACGGCGCGCTGGTGCGCGCCGACGGGGCGATCATGCTGG
GCGACAAGGTCGGTTCGATCCACCGCATCGGCGCAGTAGCGCAGGGATTGCCGGCCTGCAACGGCTGGACGTTCTGGCAC
GTCGAGACCAAGGGCGGCCTCAGGCTGATCGACGAGCTGCGCGCCGAGATCCGCTCCGAGATGAACGCCGCCTGA

Upstream 100 bases:

>100_bases
CATGGTTGAGTCGCGTCGCGGGGCGTCTGCAAGGGCGCCCCGCACTAAATTCCAGGCTCTTGAAGAGCACCGAATCATCG
TCGGCGACTGCGTCGCCGAG

Downstream 100 bases:

>100_bases
GACGTTACGACCTGCTCCGTCCCTGTCCGGAATAGCGCGCCAGGAGGTCCTCGACCTCGTACTGCTCGTCGTAGCGCGCG
TCGTCGCGCGGCACCACCGA

Product: adenine DNA methyltransferase

Products: NA

Alternate protein names: M.SmeI; Adenine-specific methyltransferase SmeIP; M.CcrMI ortholog; Modification methylase SmeIP; M.SmeIP [H]

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MSKLPGASVDLVFADPPYNLQLKGELKRPDESQVDAVDDDWDKFSSFTAYDDFTRAWLLAARRVMKPSATIWVIGSYHNI
FRVGAIMQDLGFWILNDIVWRKSNPMPNFRGRRFTNAHETMIWAARDEKAKGYTFNYEALKASNEDVQARSDWLIPLCTG
EERLKDGDGKKVHPTQKPEGLLARVLLSSSKQGDLVIDPFNGTGTTGAVAKRLGRRYIGFERDKAYAEAAERRIAAVEPL
PAETLAPFMTAREAPRVAFSELIERGMIAPGAKLVDSKRRYGALVRADGAIMLGDKVGSIHRIGAVAQGLPACNGWTFWH
VETKGGLRLIDELRAEIRSEMNAA

Sequences:

>Translated_344_residues
MSKLPGASVDLVFADPPYNLQLKGELKRPDESQVDAVDDDWDKFSSFTAYDDFTRAWLLAARRVMKPSATIWVIGSYHNI
FRVGAIMQDLGFWILNDIVWRKSNPMPNFRGRRFTNAHETMIWAARDEKAKGYTFNYEALKASNEDVQARSDWLIPLCTG
EERLKDGDGKKVHPTQKPEGLLARVLLSSSKQGDLVIDPFNGTGTTGAVAKRLGRRYIGFERDKAYAEAAERRIAAVEPL
PAETLAPFMTAREAPRVAFSELIERGMIAPGAKLVDSKRRYGALVRADGAIMLGDKVGSIHRIGAVAQGLPACNGWTFWH
VETKGGLRLIDELRAEIRSEMNAA
>Mature_343_residues
SKLPGASVDLVFADPPYNLQLKGELKRPDESQVDAVDDDWDKFSSFTAYDDFTRAWLLAARRVMKPSATIWVIGSYHNIF
RVGAIMQDLGFWILNDIVWRKSNPMPNFRGRRFTNAHETMIWAARDEKAKGYTFNYEALKASNEDVQARSDWLIPLCTGE
ERLKDGDGKKVHPTQKPEGLLARVLLSSSKQGDLVIDPFNGTGTTGAVAKRLGRRYIGFERDKAYAEAAERRIAAVEPLP
AETLAPFMTAREAPRVAFSELIERGMIAPGAKLVDSKRRYGALVRADGAIMLGDKVGSIHRIGAVAQGLPACNGWTFWHV
ETKGGLRLIDELRAEIRSEMNAA

Specific function: This methylase recognizes the double-stranded sequence GANTC and causes specific methylation on A-2 on both strands. Ccrm-mediated methylation has important cellular functions. Appears to contribute to the accurate cell-cycle control of DNA replication an

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=265, Percent_Identity=30.5660377358491, Blast_Score=107, Evalue=8e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR002052
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 38312; Mature: 38181

Theoretical pI: Translated: 9.24; Mature: 9.24

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLPGASVDLVFADPPYNLQLKGELKRPDESQVDAVDDDWDKFSSFTAYDDFTRAWLLA
CCCCCCCCEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHH
ARRVMKPSATIWVIGSYHNIFRVGAIMQDLGFWILNDIVWRKSNPMPNFRGRRFTNAHET
HHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCE
MIWAARDEKAKGYTFNYEALKASNEDVQARSDWLIPLCTGEERLKDGDGKKVHPTQKPEG
EEEEECCCCCCCEEEEHHHHCCCCCCCCCCCCCEEEEECCHHHHCCCCCCCCCCCCCCHH
LLARVLLSSSKQGDLVIDPFNGTGTTGAVAKRLGRRYIGFERDKAYAEAAERRIAAVEPL
HHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
PAETLAPFMTAREAPRVAFSELIERGMIAPGAKLVDSKRRYGALVRADGAIMLGDKVGSI
CHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHCHHHHCEEEEECCEEEECCCCCCH
HRIGAVAQGLPACNGWTFWHVETKGGLRLIDELRAEIRSEMNAA
HHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKLPGASVDLVFADPPYNLQLKGELKRPDESQVDAVDDDWDKFSSFTAYDDFTRAWLLA
CCCCCCCEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHH
ARRVMKPSATIWVIGSYHNIFRVGAIMQDLGFWILNDIVWRKSNPMPNFRGRRFTNAHET
HHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCE
MIWAARDEKAKGYTFNYEALKASNEDVQARSDWLIPLCTGEERLKDGDGKKVHPTQKPEG
EEEEECCCCCCCEEEEHHHHCCCCCCCCCCCCCEEEEECCHHHHCCCCCCCCCCCCCCHH
LLARVLLSSSKQGDLVIDPFNGTGTTGAVAKRLGRRYIGFERDKAYAEAAERRIAAVEPL
HHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
PAETLAPFMTAREAPRVAFSELIERGMIAPGAKLVDSKRRYGALVRADGAIMLGDKVGSI
CHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHCHHHHCEEEEECCEEEECCCCCCH
HRIGAVAQGLPACNGWTFWHVETKGGLRLIDELRAEIRSEMNAA
HHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9294447; 11481430 [H]