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The map label for this gene is eda [H]

Identifier: 146338867

GI number: 146338867

Start: 1930536

End: 1931180

Strand: Direct

Name: eda [H]

Synonym: BRADO1806

Alternate gene names: 146338867

Gene position: 1930536-1931180 (Clockwise)

Preceding gene: 146338866

Following gene: 146338868

Centisome position: 25.89

GC content: 69.46

Gene sequence:

>645_bases
GTGATGAGCAATCCGGACAATCGCGCGAAGCTCGATGCGGTGTTTGCGCTCGCGCCGGTGATCCCGGTGATCACGATCGA
GGACGCGGCCCAGGCCGTGCCGCTCGCGCGCGCGCTCGTCGCGGGTGGACTTCGGGTCATCGAGATCACCTTGCGGACTC
AGGCGGGCTTCGATGCCGCGCGCGCGATCATCGGCGAGGTGCCGGAGGCCAAGGTCGGCATCGGCACGGTGCTGACTCCG
GCAGACTACGAGCGCGCGGCGAAGCTCGGCGCGGCCTTCGCGATCAGTCCGGGGATGTCGATCGAGCTGCTCGACGCCGC
CAGCTCAGGCGATCTGCCGTTTGCGCCGGGCATCCAGACGCCGTCCGATCTGATCGCCTGCGTGACGCGCGGCTATGAGC
TCGTGAAGTTCTTCCCTGCTGTCCCAGCCGGCGGCCTCGCCGCGCTCGATGCATTGGCCGGACCCTTCCCGAGCGTCCGC
TTCTGTCCGACCGGTGGCATCAATGCCGCCAACGCCGCACAGTGGCTCGCGCACAAGAAGGTCGTTGCGATCGGCGGCTC
CTGGGTCGCACCGGCGGCCGACATCAAGGCCGGCGCCTGGGTCGCGATCCAGCAGCGCGCCCGCGAGGCAACGGCATTGC
GCTGA

Upstream 100 bases:

>100_bases
CGTTGGCGGGGCATCGGCTCGCGGGTGCGGTGATCATGGCGCCAGGCGCGGTGATTCCGGCGGCGGCGATGCCGCGGGAC
ATTCTGGCACTGAGCGAGGC

Downstream 100 bases:

>100_bases
TGCGTCTCTCTCCAAATCGCCTGCCGATATCCCGCCGACATCCCGCCGACATCCCGCCGACGTGGTGCTGCGCTCTGGCG
CGGCAGGCGCGCTCAGGTGC

Product: KHG/KDPG aldolase

Products: NA

Alternate protein names: 4-hydroxy-2-oxoglutarate aldolase; 2-keto-4-hydroxyglutarate aldolase; KHG-aldolase; 2-dehydro-3-deoxy-phosphogluconate aldolase; 2-keto-3-deoxy-6-phosphogluconate aldolase; KDPG-aldolase; Phospho-2-dehydro-3-deoxygluconate aldolase; Phospho-2-keto-3-deoxygluconate aldolase [H]

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MMSNPDNRAKLDAVFALAPVIPVITIEDAAQAVPLARALVAGGLRVIEITLRTQAGFDAARAIIGEVPEAKVGIGTVLTP
ADYERAAKLGAAFAISPGMSIELLDAASSGDLPFAPGIQTPSDLIACVTRGYELVKFFPAVPAGGLAALDALAGPFPSVR
FCPTGGINAANAAQWLAHKKVVAIGGSWVAPAADIKAGAWVAIQQRAREATALR

Sequences:

>Translated_214_residues
MMSNPDNRAKLDAVFALAPVIPVITIEDAAQAVPLARALVAGGLRVIEITLRTQAGFDAARAIIGEVPEAKVGIGTVLTP
ADYERAAKLGAAFAISPGMSIELLDAASSGDLPFAPGIQTPSDLIACVTRGYELVKFFPAVPAGGLAALDALAGPFPSVR
FCPTGGINAANAAQWLAHKKVVAIGGSWVAPAADIKAGAWVAIQQRAREATALR
>Mature_214_residues
MMSNPDNRAKLDAVFALAPVIPVITIEDAAQAVPLARALVAGGLRVIEITLRTQAGFDAARAIIGEVPEAKVGIGTVLTP
ADYERAAKLGAAFAISPGMSIELLDAASSGDLPFAPGIQTPSDLIACVTRGYELVKFFPAVPAGGLAALDALAGPFPSVR
FCPTGGINAANAAQWLAHKKVVAIGGSWVAPAADIKAGAWVAIQQRAREATALR

Specific function: KEY ENZYME IN THE ENTNER-DOUDOROFF PATHWAY. PARTICIPATES IN THE REGULATION OF THE INTRACELLULAR LEVEL OF GLYOXYLATE. [C]

COG id: COG0800

COG function: function code G; 2-keto-3-deoxy-6-phosphogluconate aldolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the KHG/KDPG aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1788156, Length=180, Percent_Identity=54.4444444444444, Blast_Score=185, Evalue=2e-48,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000887
- InterPro:   IPR013785 [H]

Pfam domain/function: PF01081 Aldolase [H]

EC number: =4.1.3.16; =4.1.2.14 [H]

Molecular weight: Translated: 21889; Mature: 21889

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: PS00159 ALDOLASE_KDPG_KHG_1 ; PS00160 ALDOLASE_KDPG_KHG_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSNPDNRAKLDAVFALAPVIPVITIEDAAQAVPLARALVAGGLRVIEITLRTQAGFDAA
CCCCCCCHHHHHHHHHHHHHHEEEEECHHHHHHHHHHHHHHCCEEEEEEEEECCCCHHHH
RAIIGEVPEAKVGIGTVLTPADYERAAKLGAAFAISPGMSIELLDAASSGDLPFAPGIQT
HHHHHCCCCCCCCCCEEECCCCHHHHHHHCCEEEECCCCEEEEEECCCCCCCCCCCCCCC
PSDLIACVTRGYELVKFFPAVPAGGLAALDALAGPFPSVRFCPTGGINAANAAQWLAHKK
HHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHCCCCCCCEECCCCCCCHHHHHHHHHHCE
VVAIGGSWVAPAADIKAGAWVAIQQRAREATALR
EEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MMSNPDNRAKLDAVFALAPVIPVITIEDAAQAVPLARALVAGGLRVIEITLRTQAGFDAA
CCCCCCCHHHHHHHHHHHHHHEEEEECHHHHHHHHHHHHHHCCEEEEEEEEECCCCHHHH
RAIIGEVPEAKVGIGTVLTPADYERAAKLGAAFAISPGMSIELLDAASSGDLPFAPGIQT
HHHHHCCCCCCCCCCEEECCCCHHHHHHHCCEEEECCCCEEEEEECCCCCCCCCCCCCCC
PSDLIACVTRGYELVKFFPAVPAGGLAALDALAGPFPSVRFCPTGGINAANAAQWLAHKK
HHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHCCCCCCCEECCCCCCCHHHHHHHHHHCE
VVAIGGSWVAPAADIKAGAWVAIQQRAREATALR
EEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]