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The map label for this gene is potB [H]

Identifier: 146338620

GI number: 146338620

Start: 1657054

End: 1657911

Strand: Direct

Name: potB [H]

Synonym: BRADO1548

Alternate gene names: 146338620

Gene position: 1657054-1657911 (Clockwise)

Preceding gene: 146338619

Following gene: 146338621

Centisome position: 22.22

GC content: 66.08

Gene sequence:

>858_bases
ATGAGCAGCGCACGCGCCGGCACGACGCCCTGGCTGTTGGCCGCCCCCGGCGCGCTACTGTTCACCGCGCTCGTGGTCGT
GCCGCTCGTACTTACCTTGATCCTGTCGTTCCACGTCTACGACACGGCGACTGGCGTCAGAGACGAGACCACGCTGACTC
ACTACACGACCATCCTCACCGACAGCTACTTCCTCAACATCTTCTGGCGCACCTTGCGGCTATCAGCGCTGACGACCCTG
ATCTGCGCCGTCATCGGCGCGCCCGAGGCCTACATCCTGTCGCGCATGCGCGAGCCCTGGCGTTCGATCTTCCTGCTCGC
GATCATCGGGCCGCTGCTGGTCTCGGTCGTGGTGCGGACCTTCGGCTGGAGCATGCTGCTCGGTCCTACAGGCCTCGTCA
ACGAGGCGTTCCGCGCGCTCGGCCTGGGCGCCATCAAGATCCTCTACACCGAGACCGCGATCGTCATCGCGCTGGTCCAT
GTGATGCTGCCGTTCATGGTGATCCCGGTCTGGACCTCGCTGCAGAAGCTTGATCCGATGGTGGAAGCCGCCGCCTGGAC
GCTCGGCGCCTCCCGCTTCACCGCGTTGCGCCGGGTGGTGCTGCCGCAGGTGACGATGGGTCTGCTGTCGGGCAGCCTCG
TCGTGTTCGGCCTCTCGGCGTCAGCCTTCGCGATTCCCGCGCTGCTCGGCGGCCGCCGGCTGAAGATGGCGGCGACCCTG
GTCTATGACGAATACATGCACGAGCTGAACTGGCCGCTCGGCGCCGCGATCGCGATCCTGGTGCTGGTCATCAACCTCGT
CATCTTGCTGGCCTATCAGCGTGTGGTCGAAGCCCGCGCGCGCCGGACATTGGGGTGA

Upstream 100 bases:

>100_bases
CCAATGCCGGCAAGGAGCCCGCGCGCGGGGGCGATCCGATCGGCCTCGACTGGAGCCGATCGAGCGCGAAGGCGGCCAAG
CCCGCTGCAGAACAGCCGGC

Downstream 100 bases:

>100_bases
GCGGCATGCACCGCAACGGCCCGATCGCCTTGCTGTTCCACACGCTCGTCGTCGGCTTCGTGCTGGCGCCGCTCATCGTG
ATCTGCCTCGTCGCGTTCAC

Product: ABC transporter permease

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MSSARAGTTPWLLAAPGALLFTALVVVPLVLTLILSFHVYDTATGVRDETTLTHYTTILTDSYFLNIFWRTLRLSALTTL
ICAVIGAPEAYILSRMREPWRSIFLLAIIGPLLVSVVVRTFGWSMLLGPTGLVNEAFRALGLGAIKILYTETAIVIALVH
VMLPFMVIPVWTSLQKLDPMVEAAAWTLGASRFTALRRVVLPQVTMGLLSGSLVVFGLSASAFAIPALLGGRRLKMAATL
VYDEYMHELNWPLGAAIAILVLVINLVILLAYQRVVEARARRTLG

Sequences:

>Translated_285_residues
MSSARAGTTPWLLAAPGALLFTALVVVPLVLTLILSFHVYDTATGVRDETTLTHYTTILTDSYFLNIFWRTLRLSALTTL
ICAVIGAPEAYILSRMREPWRSIFLLAIIGPLLVSVVVRTFGWSMLLGPTGLVNEAFRALGLGAIKILYTETAIVIALVH
VMLPFMVIPVWTSLQKLDPMVEAAAWTLGASRFTALRRVVLPQVTMGLLSGSLVVFGLSASAFAIPALLGGRRLKMAATL
VYDEYMHELNWPLGAAIAILVLVINLVILLAYQRVVEARARRTLG
>Mature_284_residues
SSARAGTTPWLLAAPGALLFTALVVVPLVLTLILSFHVYDTATGVRDETTLTHYTTILTDSYFLNIFWRTLRLSALTTLI
CAVIGAPEAYILSRMREPWRSIFLLAIIGPLLVSVVVRTFGWSMLLGPTGLVNEAFRALGLGAIKILYTETAIVIALVHV
MLPFMVIPVWTSLQKLDPMVEAAAWTLGASRFTALRRVVLPQVTMGLLSGSLVVFGLSASAFAIPALLGGRRLKMAATLV
YDEYMHELNWPLGAAIAILVLVINLVILLAYQRVVEARARRTLG

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=253, Percent_Identity=31.6205533596838, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1787080, Length=226, Percent_Identity=29.2035398230088, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI1787713, Length=258, Percent_Identity=26.3565891472868, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788764, Length=240, Percent_Identity=25, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31076; Mature: 30945

Theoretical pI: Translated: 10.18; Mature: 10.18

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSARAGTTPWLLAAPGALLFTALVVVPLVLTLILSFHVYDTATGVRDETTLTHYTTILT
CCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
DSYFLNIFWRTLRLSALTTLICAVIGAPEAYILSRMREPWRSIFLLAIIGPLLVSVVVRT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGWSMLLGPTGLVNEAFRALGLGAIKILYTETAIVIALVHVMLPFMVIPVWTSLQKLDPM
HCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VEAAAWTLGASRFTALRRVVLPQVTMGLLSGSLVVFGLSASAFAIPALLGGRRLKMAATL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCHHHHHHHHHHCCCHHHHHHHH
VYDEYMHELNWPLGAAIAILVLVINLVILLAYQRVVEARARRTLG
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SSARAGTTPWLLAAPGALLFTALVVVPLVLTLILSFHVYDTATGVRDETTLTHYTTILT
CCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
DSYFLNIFWRTLRLSALTTLICAVIGAPEAYILSRMREPWRSIFLLAIIGPLLVSVVVRT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGWSMLLGPTGLVNEAFRALGLGAIKILYTETAIVIALVHVMLPFMVIPVWTSLQKLDPM
HCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VEAAAWTLGASRFTALRRVVLPQVTMGLLSGSLVVFGLSASAFAIPALLGGRRLKMAATL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCHHHHHHHHHHCCCHHHHHHHH
VYDEYMHELNWPLGAAIAILVLVINLVILLAYQRVVEARARRTLG
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]