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The map label for this gene is amdA

Identifier: 146338603

GI number: 146338603

Start: 1635162

End: 1636667

Strand: Direct

Name: amdA

Synonym: BRADO1531

Alternate gene names: NA

Gene position: 1635162-1636667 (Clockwise)

Preceding gene: 146338602

Following gene: 146338605

Centisome position: 21.93

GC content: 66.14

Gene sequence:

>1506_bases
ATGCCAGTCCGCCGTCCGACGGCACCCGAGTTGCAGCAAGCTGCGCGTGAGATCGGCCTCGATCTCGATGATGCCGATGC
GCTGTCGTTTCTCGCGCTGATGCAGGGCCAGCTCGACGCCTATGATCTCGTCGATGGCATGACCGCTCCGCTGCCTGAGG
TCAAATATCCAAGACCTCCGGGCGTCCGTCCGGCGCCGGAGGCCAATCCGTTCGGCGCCTGGGCGGTCACCACACATATT
CGTGGCGCTTCGGAGGGGAAGCTCAAGGGCAAGCGCGTCGCCATCAAGGACAATGTCTGCGTTGCCGGCGTGCAGATGAT
GAACGGCGCCACCATTCTCGAAGGCTACGTGCCGGAGATGGATGCGACCATCGTCACGCGTCTGCTGGACGCAGGCGCCG
ATATCCTCGGCAAGGCGGTCTGCGAGTATTACTGCGCCTCCGGCGGCAGTCACACCTCCGCCAATGGCGTCGTCGAAAAT
CCTGTTGTGCCCGGCTACAATGCCGGCGGATCGTCATCGGGATCCACGGCCCTGGTGATGGCTGATATCGTCGACATGGC
GACCGGAGGCGATCAGGGCGGCTCGATCCGAATTCCCGCGTCTTATTGTGGCGCGGTCGGTCTCAAGCCCACGCACGGCC
TCGTGCCTTATACGGGAATTTTTGCAGTCGAGCTCACCGTCGATCACGCCGGGCCGATCACCCGCACCGTGGCGGACAAT
GCGCTGATGCTGGAAGTCATGGCGGGGCCCGATGGCCTCGATCCGCGGCAGCGTGGCGCGGCCGCGCAGCCCTACACACA
GGCACTGTCGCAAGGCGCGGCAGGGTTGCGGATCGGCATCGTGCCCGAAGGGTTCGGCACGGCCGGCGCCGAGAGCGAGG
TCGACGACCGTGTGCGCGAGGCGGCGGGCCGCCTCCAGGCGAAGGGGGCGGATATTCGCGAGGTCTCCGTCCCGCTCCAT
GCCGCCGGCGCGGCGATTTGGACGCCAATCTTTCTCGAAGGCGCCACCGATCTGATGATGCGTGGCAACGCCTATGGCAC
CAACATGAAAGGCGTGTTTCTCGAAAGTCTGCTCGATGCCCATGCGCGCTGGCGCGATCGGGCGGGCGAGCTGTCGGACA
CGCTGAAGCTCGGCATCCTGACCGGCCACTACATGTCGTCGCGCAACCGTGGCCGCTACTACGGCAAGGCGCAGAACCTC
AACCGTCTGCTCACGGCAGATTATGATCGCGCGCTCGGGCAGGCCGACGTGCTGCTGATGCCGACCACGCCGATGGCGGC
GACGCGGCTGCCTGCCGCCGGAGCGTCGCGCGAGGAGATCATCGGCCGTGCCTTCGAGATGGTCGGCAACACCGCGCCGA
CCTGTCTCACCGGCCATCCCGCGATTTCCGTTCCCGTCGGAAAGACCTCCGACGGCCGGCCGATCGGCGCGATGCTGATC
GCGCGTCATCTCGACGAGATGACGCTGTACCGCGCCGCCGCGGCGCTGGAGGCGTGCTATCAGTAG

Upstream 100 bases:

>100_bases
GGAATGACCTCAGGGCTGGCTAGTCTCATCGCACAATGAACGCTGATGTCCACCCGCATCGTTGCGGATGGACCGGTTCT
CACCTTGACGAGAGGCATCG

Downstream 100 bases:

>100_bases
GGATGCTGCGGATCGGTCTACGGCAGCAGTGGCAACAGCCCAGCGAGCTCAGACAATCCCCGGATCCGGTGGTCGGGCGC
GAGCCCGAGCTCGTCCATCT

Product: amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 501; Mature: 500

Protein sequence:

>501_residues
MPVRRPTAPELQQAAREIGLDLDDADALSFLALMQGQLDAYDLVDGMTAPLPEVKYPRPPGVRPAPEANPFGAWAVTTHI
RGASEGKLKGKRVAIKDNVCVAGVQMMNGATILEGYVPEMDATIVTRLLDAGADILGKAVCEYYCASGGSHTSANGVVEN
PVVPGYNAGGSSSGSTALVMADIVDMATGGDQGGSIRIPASYCGAVGLKPTHGLVPYTGIFAVELTVDHAGPITRTVADN
ALMLEVMAGPDGLDPRQRGAAAQPYTQALSQGAAGLRIGIVPEGFGTAGAESEVDDRVREAAGRLQAKGADIREVSVPLH
AAGAAIWTPIFLEGATDLMMRGNAYGTNMKGVFLESLLDAHARWRDRAGELSDTLKLGILTGHYMSSRNRGRYYGKAQNL
NRLLTADYDRALGQADVLLMPTTPMAATRLPAAGASREEIIGRAFEMVGNTAPTCLTGHPAISVPVGKTSDGRPIGAMLI
ARHLDEMTLYRAAAALEACYQ

Sequences:

>Translated_501_residues
MPVRRPTAPELQQAAREIGLDLDDADALSFLALMQGQLDAYDLVDGMTAPLPEVKYPRPPGVRPAPEANPFGAWAVTTHI
RGASEGKLKGKRVAIKDNVCVAGVQMMNGATILEGYVPEMDATIVTRLLDAGADILGKAVCEYYCASGGSHTSANGVVEN
PVVPGYNAGGSSSGSTALVMADIVDMATGGDQGGSIRIPASYCGAVGLKPTHGLVPYTGIFAVELTVDHAGPITRTVADN
ALMLEVMAGPDGLDPRQRGAAAQPYTQALSQGAAGLRIGIVPEGFGTAGAESEVDDRVREAAGRLQAKGADIREVSVPLH
AAGAAIWTPIFLEGATDLMMRGNAYGTNMKGVFLESLLDAHARWRDRAGELSDTLKLGILTGHYMSSRNRGRYYGKAQNL
NRLLTADYDRALGQADVLLMPTTPMAATRLPAAGASREEIIGRAFEMVGNTAPTCLTGHPAISVPVGKTSDGRPIGAMLI
ARHLDEMTLYRAAAALEACYQ
>Mature_500_residues
PVRRPTAPELQQAAREIGLDLDDADALSFLALMQGQLDAYDLVDGMTAPLPEVKYPRPPGVRPAPEANPFGAWAVTTHIR
GASEGKLKGKRVAIKDNVCVAGVQMMNGATILEGYVPEMDATIVTRLLDAGADILGKAVCEYYCASGGSHTSANGVVENP
VVPGYNAGGSSSGSTALVMADIVDMATGGDQGGSIRIPASYCGAVGLKPTHGLVPYTGIFAVELTVDHAGPITRTVADNA
LMLEVMAGPDGLDPRQRGAAAQPYTQALSQGAAGLRIGIVPEGFGTAGAESEVDDRVREAAGRLQAKGADIREVSVPLHA
AGAAIWTPIFLEGATDLMMRGNAYGTNMKGVFLESLLDAHARWRDRAGELSDTLKLGILTGHYMSSRNRGRYYGKAQNLN
RLLTADYDRALGQADVLLMPTTPMAATRLPAAGASREEIIGRAFEMVGNTAPTCLTGHPAISVPVGKTSDGRPIGAMLIA
RHLDEMTLYRAAAALEACYQ

Specific function: Unknown

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=498, Percent_Identity=28.3132530120482, Blast_Score=159, Evalue=7e-39,
Organism=Homo sapiens, GI195972892, Length=401, Percent_Identity=24.4389027431421, Blast_Score=77, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17543272, Length=413, Percent_Identity=26.8765133171913, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17537465, Length=455, Percent_Identity=26.8131868131868, Blast_Score=72, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6323950, Length=466, Percent_Identity=27.0386266094421, Blast_Score=100, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24648113, Length=431, Percent_Identity=32.4825986078886, Blast_Score=170, Evalue=2e-42,
Organism=Drosophila melanogaster, GI161078093, Length=435, Percent_Identity=27.3563218390805, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24644968, Length=379, Percent_Identity=25.8575197889182, Blast_Score=90, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.1.4 [H]

Molecular weight: Translated: 52465; Mature: 52334

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVRRPTAPELQQAAREIGLDLDDADALSFLALMQGQLDAYDLVDGMTAPLPEVKYPRPP
CCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCC
GVRPAPEANPFGAWAVTTHIRGASEGKLKGKRVAIKDNVCVAGVQMMNGATILEGYVPEM
CCCCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEEECCEEEECHHHHCCCEEEECCCCCC
DATIVTRLLDAGADILGKAVCEYYCASGGSHTSANGVVENPVVPGYNAGGSSSGSTALVM
HHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEH
ADIVDMATGGDQGGSIRIPASYCGAVGLKPTHGLVPYTGIFAVELTVDHAGPITRTVADN
HHHHHHHCCCCCCCEEEECHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCHHHCCC
ALMLEVMAGPDGLDPRQRGAAAQPYTQALSQGAAGLRIGIVPEGFGTAGAESEVDDRVRE
EEEEEEECCCCCCCHHHCCCCCCHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHH
AAGRLQAKGADIREVSVPLHAAGAAIWTPIFLEGATDLMMRGNAYGTNMKGVFLESLLDA
HHHHHHCCCCCCEEEECCHHHCCHHHHHHHHHCCCHHHEECCCCCCCCCHHHHHHHHHHH
HARWRDRAGELSDTLKLGILTGHYMSSRNRGRYYGKAQNLNRLLTADYDRALGQADVLLM
HHHHHHHCCCHHHHEEEEEEECHHHCCCCCCCEECCHHCCHHHHHCCHHHHCCCCCEEEE
PTTPMAATRLPAAGASREEIIGRAFEMVGNTAPTCLTGHPAISVPVGKTSDGRPIGAMLI
CCCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCEEEECCCCCCCCCCHHHHHH
ARHLDEMTLYRAAAALEACYQ
HHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PVRRPTAPELQQAAREIGLDLDDADALSFLALMQGQLDAYDLVDGMTAPLPEVKYPRPP
CCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCC
GVRPAPEANPFGAWAVTTHIRGASEGKLKGKRVAIKDNVCVAGVQMMNGATILEGYVPEM
CCCCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEEECCEEEECHHHHCCCEEEECCCCCC
DATIVTRLLDAGADILGKAVCEYYCASGGSHTSANGVVENPVVPGYNAGGSSSGSTALVM
HHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEH
ADIVDMATGGDQGGSIRIPASYCGAVGLKPTHGLVPYTGIFAVELTVDHAGPITRTVADN
HHHHHHHCCCCCCCEEEECHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCHHHCCC
ALMLEVMAGPDGLDPRQRGAAAQPYTQALSQGAAGLRIGIVPEGFGTAGAESEVDDRVRE
EEEEEEECCCCCCCHHHCCCCCCHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHH
AAGRLQAKGADIREVSVPLHAAGAAIWTPIFLEGATDLMMRGNAYGTNMKGVFLESLLDA
HHHHHHCCCCCCEEEECCHHHCCHHHHHHHHHCCCHHHEECCCCCCCCCHHHHHHHHHHH
HARWRDRAGELSDTLKLGILTGHYMSSRNRGRYYGKAQNLNRLLTADYDRALGQADVLLM
HHHHHHHCCCHHHHEEEEEEECHHHCCCCCCCEECCHHCCHHHHHCCHHHHCCCCCEEEE
PTTPMAATRLPAAGASREEIIGRAFEMVGNTAPTCLTGHPAISVPVGKTSDGRPIGAMLI
CCCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCEEEECCCCCCCCCCHHHHHH
ARHLDEMTLYRAAAALEACYQ
HHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2013568 [H]