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The map label for this gene is linC [H]

Identifier: 146338517

GI number: 146338517

Start: 1535631

End: 1536425

Strand: Direct

Name: linC [H]

Synonym: BRADO1435

Alternate gene names: 146338517

Gene position: 1535631-1536425 (Clockwise)

Preceding gene: 146338516

Following gene: 146338518

Centisome position: 20.59

GC content: 66.54

Gene sequence:

>795_bases
ATGCAGGTGACCGGCAAGATTGTAGTGGTCACCGGTGGAGCCCGTGGCATCGGCAAGGCGCTGTGCGAGGCGTTTGCCCG
CGACGGCGCAGCCAAGGTGATCGTGGCAGATCTCGACGAGGCCGGCGCCAAGGCGGTCGCTGACAGCATCGGCGGCGCTT
CGTTCCGCTGCGACGTCTCGCAGGAAGCCGACATCAAGCACCTCATCGAGGAGACCGAGCGGCAGTTCGGGCCGATCGCA
TTGTTCTGCTCCAATGCCGGCATCGGCGGCGGCTTCGATCCATTGTCGGTGAATGTCGGCGGCACCTCCGACGAGCCGTG
GGCGCGCAGCTGGGCCATTCACGTGATGGCCCATGTCTGGGCGGCGCGGCACCTGATCCCGCGCTACAAGGCGCGCGGCG
GCGGATACTTCCTCAACACGATCTCCGCGGCCGGCCTGTTGTCCCAGGTCGGCAGCGCGCCTTACTCGACCACCAAGCAC
GCCGCCGTCGGCTTCGCCGAGAATCTGGCGATCTCGCACAAGGTCGACAATATCAAGGTCTCGATCCTCTGCCCGCAGGG
TGTCGACACCGACATGCTGCGCGCGATCCCCAAGGGCCCCCAATCCGGCGACGGCGATCTCACGCCGGAGCAGGTCGCGC
AGGACGTGCTGAAGGGCCTCGCCGAGGAGAGCTTCCTGATCCTGCCGCATCCCCAGGTGCTCGGCTACATGCGCAAGAAG
ACCGAGAACTACGACCGCTGGCTCGGCGGCATGGCCAAGATCCAGGCCAGGATGCGCGAGGAGTTCGGGACGTAG

Upstream 100 bases:

>100_bases
TGGTTTCGTGTTGCTGGGCGCCAGTTTCCATTATACGGCGGTGCTCGATCTCGTCCTGACATAGATCATTCCAGGACAAA
TTCCGTGCGGAGGGCTTTGC

Downstream 100 bases:

>100_bases
GCGGAGGCGATGCATGCCTGCGGTGGGTGGACGACGTCCATCAACACCCTCCGCTGTCGTCCCGGCGAAAGCCGGGACCC
ATAACCCCAGGAAGTAGTTC

Product: putative short-chain dehydrogenase/reductase

Products: (3R)-hydroxyacyl-[acyl-carrier-protein]; NADP; NADPH; Proton; beta-ketoacyl-ACP [C]

Alternate protein names: 2,5-DDOL dehydrogenase [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MQVTGKIVVVTGGARGIGKALCEAFARDGAAKVIVADLDEAGAKAVADSIGGASFRCDVSQEADIKHLIEETERQFGPIA
LFCSNAGIGGGFDPLSVNVGGTSDEPWARSWAIHVMAHVWAARHLIPRYKARGGGYFLNTISAAGLLSQVGSAPYSTTKH
AAVGFAENLAISHKVDNIKVSILCPQGVDTDMLRAIPKGPQSGDGDLTPEQVAQDVLKGLAEESFLILPHPQVLGYMRKK
TENYDRWLGGMAKIQARMREEFGT

Sequences:

>Translated_264_residues
MQVTGKIVVVTGGARGIGKALCEAFARDGAAKVIVADLDEAGAKAVADSIGGASFRCDVSQEADIKHLIEETERQFGPIA
LFCSNAGIGGGFDPLSVNVGGTSDEPWARSWAIHVMAHVWAARHLIPRYKARGGGYFLNTISAAGLLSQVGSAPYSTTKH
AAVGFAENLAISHKVDNIKVSILCPQGVDTDMLRAIPKGPQSGDGDLTPEQVAQDVLKGLAEESFLILPHPQVLGYMRKK
TENYDRWLGGMAKIQARMREEFGT
>Mature_264_residues
MQVTGKIVVVTGGARGIGKALCEAFARDGAAKVIVADLDEAGAKAVADSIGGASFRCDVSQEADIKHLIEETERQFGPIA
LFCSNAGIGGGFDPLSVNVGGTSDEPWARSWAIHVMAHVWAARHLIPRYKARGGGYFLNTISAAGLLSQVGSAPYSTTKH
AAVGFAENLAISHKVDNIKVSILCPQGVDTDMLRAIPKGPQSGDGDLTPEQVAQDVLKGLAEESFLILPHPQVLGYMRKK
TENYDRWLGGMAKIQARMREEFGT

Specific function: Catalyzes the degradation of 2,5-dichloro-2,5- cyclohexadiene-1,4-diol (2,5-DDOL) into 2,5-dichlorohydroquinone (2,5-DCHQ) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI40807363, Length=245, Percent_Identity=27.3469387755102, Blast_Score=101, Evalue=8e-22,
Organism=Homo sapiens, GI142976729, Length=237, Percent_Identity=27.4261603375527, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI310128830, Length=256, Percent_Identity=26.171875, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI310115468, Length=256, Percent_Identity=26.171875, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI40254992, Length=241, Percent_Identity=28.6307053941909, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI210032110, Length=245, Percent_Identity=24.0816326530612, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI32483357, Length=198, Percent_Identity=26.7676767676768, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI31542939, Length=216, Percent_Identity=27.3148148148148, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI25282469, Length=262, Percent_Identity=22.9007633587786, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI4503817, Length=236, Percent_Identity=26.6949152542373, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI59889578, Length=170, Percent_Identity=28.8235294117647, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI33667109, Length=199, Percent_Identity=27.1356783919598, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI5031737, Length=194, Percent_Identity=27.319587628866, Blast_Score=71, Evalue=8e-13,
Organism=Homo sapiens, GI126723191, Length=185, Percent_Identity=27.5675675675676, Blast_Score=70, Evalue=1e-12,
Organism=Escherichia coli, GI1787335, Length=205, Percent_Identity=29.7560975609756, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1787905, Length=226, Percent_Identity=28.7610619469027, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI87082100, Length=229, Percent_Identity=29.6943231441048, Blast_Score=76, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17564282, Length=206, Percent_Identity=29.126213592233, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17508895, Length=235, Percent_Identity=26.8085106382979, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI193203117, Length=237, Percent_Identity=24.0506329113924, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI193203115, Length=235, Percent_Identity=24.2553191489362, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17538182, Length=213, Percent_Identity=28.6384976525822, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17555706, Length=200, Percent_Identity=28.5, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17561402, Length=218, Percent_Identity=28.4403669724771, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI25147288, Length=200, Percent_Identity=30, Blast_Score=71, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17567345, Length=200, Percent_Identity=26.5, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6322861, Length=197, Percent_Identity=29.4416243654822, Blast_Score=69, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24581726, Length=243, Percent_Identity=24.6913580246914, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24640492, Length=220, Percent_Identity=26.8181818181818, Blast_Score=78, Evalue=7e-15,
Organism=Drosophila melanogaster, GI281364892, Length=261, Percent_Identity=25.6704980842912, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24649181, Length=258, Percent_Identity=25.1937984496124, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21355319, Length=213, Percent_Identity=26.2910798122066, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21356301, Length=235, Percent_Identity=28.0851063829787, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI21355631, Length=173, Percent_Identity=24.8554913294798, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI221510726, Length=213, Percent_Identity=25.8215962441315, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.100 [C]

Molecular weight: Translated: 28045; Mature: 28045

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVTGKIVVVTGGARGIGKALCEAFARDGAAKVIVADLDEAGAKAVADSIGGASFRCDVS
CCCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHCCCCEEEECCC
QEADIKHLIEETERQFGPIALFCSNAGIGGGFDPLSVNVGGTSDEPWARSWAIHVMAHVW
CCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHH
AARHLIPRYKARGGGYFLNTISAAGLLSQVGSAPYSTTKHAAVGFAENLAISHKVDNIKV
HHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCEEEEEECCEEE
SILCPQGVDTDMLRAIPKGPQSGDGDLTPEQVAQDVLKGLAEESFLILPHPQVLGYMRKK
EEECCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHH
TENYDRWLGGMAKIQARMREEFGT
CCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQVTGKIVVVTGGARGIGKALCEAFARDGAAKVIVADLDEAGAKAVADSIGGASFRCDVS
CCCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHCCCCEEEECCC
QEADIKHLIEETERQFGPIALFCSNAGIGGGFDPLSVNVGGTSDEPWARSWAIHVMAHVW
CCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHH
AARHLIPRYKARGGGYFLNTISAAGLLSQVGSAPYSTTKHAAVGFAENLAISHKVDNIKV
HHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCEEEEEECCEEE
SILCPQGVDTDMLRAIPKGPQSGDGDLTPEQVAQDVLKGLAEESFLILPHPQVLGYMRKK
EEECCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHH
TENYDRWLGGMAKIQARMREEFGT
CCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Oxoacyl-[acyl-carrier-protein]; NADPH; D-3-hydroxy-acyl-ACP; NADP [C]

Specific reaction: Oxoacyl-[acyl-carrier-protein] + NADPH = (3R)-hydroxyacyl-[acyl-carrier-protein] + NADP+ D-3-hydroxy-acyl-ACP + NADP = NADPH + Proton + beta-ketoacyl-ACP [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7515041; 12450824 [H]