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The map label for this gene is purK [H]

Identifier: 146338301

GI number: 146338301

Start: 1280830

End: 1281939

Strand: Reverse

Name: purK [H]

Synonym: BRADO1205

Alternate gene names: 146338301

Gene position: 1281939-1280830 (Counterclockwise)

Preceding gene: 146338302

Following gene: 146338300

Centisome position: 17.19

GC content: 66.31

Gene sequence:

>1110_bases
GTGACCGGCTCCAATCGGGTGAAGCTCAAGCCCGGGGACACCATCGGCATTCTCGGCGGCGGCCAGCTCGGCCGCATGCT
GGCGTTGGCGGCCGCGCGGCTCGGACTGCGTTGCCAGGTGTTCTCGCCCGACCCTGACTCGCCGGCCTTTGACGTGGTCC
TGAACGCCACCTGCGCCGAATATGCGGACGTCGAGGCGCTGGAATTGTTCGCGAACGACGTCGACGTCGTCACCTACGAG
TTCGAAAACGTCCCGTCGGCGGCGGCGCTGGTCCTGGCCGCACGACGTCCCGTGCTGCCCAGCCGCTCCGCGCTGGAGAC
CACCCAGGACCGACTGACGGAAAAGGATTTCGTCACCTCGCTGGGCATCCACACCGCCAATTACGCCGACGTCTCGTCGG
TCGGCACATTGCGAGAGGCCATCCTGCGCATCGGCCTGCCGGCCGTGCTGAAAACCCGCCGCTTCGGCTACGACGGCAAG
GGTCAGGTCAAGATCCGCCAGGGCGACGACCTCGAACGGATCTGGGCCGAGCTCGGCACCAAATCCGCCATCCTCGAGGC
CTTCGTGCCCTTCGAGCGCGAGATCTCCGTAGTGGCTGCGCGTGCCGCCGATGGCCAGGTCGAATGCTTCGACGTCACCG
AGAACGAGCATCGCGATCACATTCTCAAGGTGTCGCGGGCACCCGCTTCCATTCCGGACGCGCTCGCTGACGAGGCCCGG
GACATCGCCAGCCGGATCGCGACGGCACTGGACTATGTGGGGGTCCTCGCGGTGGAGATGTTCGTCGTGACCGCTGGGCC
TAAACCCGGCGTGGTTGTCAACGAGATTGCGCCACGGGTGCACAATTCCGGCCATTGGACGCTCGACGGCGCGTCCATCT
CGCAGTTCGAACAGCACATCCGGGCGATCGCTGGCTGGCCTCTCGGCAAACCAGTGCGCCATGGCCATGTCACGATGACC
AATCTGATCGGCGATGAGATCGACAGCTATGAGCAGTGGCTGACCGTTCCCGGAGCGACCGTTCACCTCTATGGCAAGGG
CGCCCCCCGCCCGGGCCGCAAGATGGGCCATGTGACGCAGGTCTCGCCGCTCCCGCCCCGGATGGGCTGA

Upstream 100 bases:

>100_bases
CAAGCGTCCTGGCGCTGTCGGACCCGGCGCTGGCCGAGCGTCTCTCCGCCTGGCGGCAGTCGCAGACCAACGCCATCGCC
GAACGTCCGGAGGGCGCGGC

Downstream 100 bases:

>100_bases
AATATACCAGATCTGAAGTGCTAACTTTGGCGTTAAGAGCCTATCAGCGGGTGGACAGCGTTGCGCATGTCTGATACACG
GGCGCCCTCAAGGTTAAGGG

Product: phosphoribosylaminoimidazole carboxylase ATPase subunit

Products: NA

Alternate protein names: N5-CAIR synthase; 5-(carboxyamino)imidazole ribonucleotide synthetase [H]

Number of amino acids: Translated: 369; Mature: 368

Protein sequence:

>369_residues
MTGSNRVKLKPGDTIGILGGGQLGRMLALAAARLGLRCQVFSPDPDSPAFDVVLNATCAEYADVEALELFANDVDVVTYE
FENVPSAAALVLAARRPVLPSRSALETTQDRLTEKDFVTSLGIHTANYADVSSVGTLREAILRIGLPAVLKTRRFGYDGK
GQVKIRQGDDLERIWAELGTKSAILEAFVPFEREISVVAARAADGQVECFDVTENEHRDHILKVSRAPASIPDALADEAR
DIASRIATALDYVGVLAVEMFVVTAGPKPGVVVNEIAPRVHNSGHWTLDGASISQFEQHIRAIAGWPLGKPVRHGHVTMT
NLIGDEIDSYEQWLTVPGATVHLYGKGAPRPGRKMGHVTQVSPLPPRMG

Sequences:

>Translated_369_residues
MTGSNRVKLKPGDTIGILGGGQLGRMLALAAARLGLRCQVFSPDPDSPAFDVVLNATCAEYADVEALELFANDVDVVTYE
FENVPSAAALVLAARRPVLPSRSALETTQDRLTEKDFVTSLGIHTANYADVSSVGTLREAILRIGLPAVLKTRRFGYDGK
GQVKIRQGDDLERIWAELGTKSAILEAFVPFEREISVVAARAADGQVECFDVTENEHRDHILKVSRAPASIPDALADEAR
DIASRIATALDYVGVLAVEMFVVTAGPKPGVVVNEIAPRVHNSGHWTLDGASISQFEQHIRAIAGWPLGKPVRHGHVTMT
NLIGDEIDSYEQWLTVPGATVHLYGKGAPRPGRKMGHVTQVSPLPPRMG
>Mature_368_residues
TGSNRVKLKPGDTIGILGGGQLGRMLALAAARLGLRCQVFSPDPDSPAFDVVLNATCAEYADVEALELFANDVDVVTYEF
ENVPSAAALVLAARRPVLPSRSALETTQDRLTEKDFVTSLGIHTANYADVSSVGTLREAILRIGLPAVLKTRRFGYDGKG
QVKIRQGDDLERIWAELGTKSAILEAFVPFEREISVVAARAADGQVECFDVTENEHRDHILKVSRAPASIPDALADEARD
IASRIATALDYVGVLAVEMFVVTAGPKPGVVVNEIAPRVHNSGHWTLDGASISQFEQHIRAIAGWPLGKPVRHGHVTMTN
LIGDEIDSYEQWLTVPGATVHLYGKGAPRPGRKMGHVTQVSPLPPRMG

Specific function: Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR) [H]

COG id: COG0026

COG function: function code F; Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786733, Length=345, Percent_Identity=37.1014492753623, Blast_Score=189, Evalue=4e-49,
Organism=Escherichia coli, GI1788155, Length=365, Percent_Identity=26.8493150684932, Blast_Score=102, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6324702, Length=370, Percent_Identity=32.4324324324324, Blast_Score=197, Evalue=2e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005875
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp [H]

EC number: =6.3.4.18 [H]

Molecular weight: Translated: 39736; Mature: 39605

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGSNRVKLKPGDTIGILGGGQLGRMLALAAARLGLRCQVFSPDPDSPAFDVVLNATCAE
CCCCCEEEECCCCEEEEECCCHHHHHHHHHHHHHCCEEEEECCCCCCCCEEEEEECHHHH
YADVEALELFANDVDVVTYEFENVPSAAALVLAARRPVLPSRSALETTQDRLTEKDFVTS
HCCHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
LGIHTANYADVSSVGTLREAILRIGLPAVLKTRRFGYDGKGQVKIRQGDDLERIWAELGT
HCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHCC
KSAILEAFVPFEREISVVAARAADGQVECFDVTENEHRDHILKVSRAPASIPDALADEAR
HHHHHHHHCCCCCCEEEEEEECCCCCEEEEECCCCCCCHHEEEECCCCCCCCHHHHHHHH
DIASRIATALDYVGVLAVEMFVVTAGPKPGVVVNEIAPRVHNSGHWTLDGASISQFEQHI
HHHHHHHHHHHHHHHHHHHHEEEECCCCCCEEHHHHHHHHCCCCCEEECCCCHHHHHHHH
RAIAGWPLGKPVRHGHVTMTNLIGDEIDSYEQWLTVPGATVHLYGKGAPRPGRKMGHVTQ
HHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHHHEECCCCEEEEEECCCCCCCCCCCCEEE
VSPLPPRMG
CCCCCCCCC
>Mature Secondary Structure 
TGSNRVKLKPGDTIGILGGGQLGRMLALAAARLGLRCQVFSPDPDSPAFDVVLNATCAE
CCCCEEEECCCCEEEEECCCHHHHHHHHHHHHHCCEEEEECCCCCCCCEEEEEECHHHH
YADVEALELFANDVDVVTYEFENVPSAAALVLAARRPVLPSRSALETTQDRLTEKDFVTS
HCCHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
LGIHTANYADVSSVGTLREAILRIGLPAVLKTRRFGYDGKGQVKIRQGDDLERIWAELGT
HCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHCC
KSAILEAFVPFEREISVVAARAADGQVECFDVTENEHRDHILKVSRAPASIPDALADEAR
HHHHHHHHCCCCCCEEEEEEECCCCCEEEEECCCCCCCHHEEEECCCCCCCCHHHHHHHH
DIASRIATALDYVGVLAVEMFVVTAGPKPGVVVNEIAPRVHNSGHWTLDGASISQFEQHI
HHHHHHHHHHHHHHHHHHHHEEEECCCCCCEEHHHHHHHHCCCCCEEECCCCHHHHHHHH
RAIAGWPLGKPVRHGHVTMTNLIGDEIDSYEQWLTVPGATVHLYGKGAPRPGRKMGHVTQ
HHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHHHEECCCCEEEEEECCCCCCCCCCCCEEE
VSPLPPRMG
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]