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The map label for this gene is tilS [H]

Identifier: 146338275

GI number: 146338275

Start: 1250266

End: 1251369

Strand: Direct

Name: tilS [H]

Synonym: BRADO1179

Alternate gene names: 146338275

Gene position: 1250266-1251369 (Clockwise)

Preceding gene: 146338274

Following gene: 146338276

Centisome position: 16.77

GC content: 69.84

Gene sequence:

>1104_bases
ATGGCAGCCCACGAGACCGCGCAGGCGAATCCTTCCGACGACGGCCGTCCCATCGGCGTGGCCGAAGCGGAGGTGCTGTT
CGCTTCGCTCGCCGATGCGCCTGCGCTGGTGCTTGCCGTGTCCGGCGGCCCCGATTCGATGGCGCTGCTATGGCTGGCGG
CTCGCTGGCGGCAGGCGCTGGCAGACGGGCCGCGACTGGTTGCAATCACCGTCGATCATGGCTTGCGGCCGGAAGCCCGG
CGCGAAGCTGCGATGGTCAAGCGGTTCGCGCGGCAGCTCGAGGTTTCGCACCGGATGCTGCGCTGGACCGAGCCGAAGCC
GACCACGGGAATTCCCGAAGCGGCGCGTCTGGCCAGATATCGCCTGCTGGCACGGGCTGCGCGGCAGGCCGGTGCAAGCC
ACGTCCTCACCGCTCACACCCGCGACGATCAGGCCGAGACGGTGCTGATGCGGTTGCTGCGTGGCAGCGGCATCGTCGGG
CTCGCGGCCATGGCACCGGTCAGCGCCCGCGACGGGCTGCTGCTGGCGCGCCCGCTGCTGGAGATTTCGAAGGCCCGGCT
CGTCGCCACCCTTCGCTCGGCCGGGATCGAGTTTGCCGATGACCCCACCAACCACGATCCGGCCTATACGCGGCCGCGCT
TGCGCGCGCTGATGCCGGCGCTGGCAGCCGAGGGTGGTGATTCGCGGACGCTGGCGACGCTCGCCGGCCGCCTCGCCCGC
GCCAACGCGGCCATCGAGCTGATGGTCGATGGTGCCGAGAAGCACCTCGCGCTCCGCGCTTCGCGCGGCGGCTCGATCCG
GCCTGCCCAGGGGCAGACGTTCGAGGCGCGCGCCTTTGTCGCACTTCCGGCGGAGATCAGGCTGAGATTGCTGATGCGGG
CCATCAACCAGATTGGAACCGAGGGGCCGGTGGAACTCGGGAAGGCGGAAGCGCTGCTCGATCGCATGGACCGGACGATG
GACCGGACTCTTGCGGGGGTCGCAAATGGTGGTACCTCTGCGGCGGGCAGGCTAAAACAAACCTTGGCTGGTGCGCTGGT
AAGCATGACGCGCGAGGCCATTCGCATCACTCCGGCGCCGCCTCGTCGTCCGCGCCGGAGTTGA

Upstream 100 bases:

>100_bases
AGGAGCAGAAGCGCGTGAAGTGCTGACCGCGCGCGGCGCTTGTCGCGCGCGAAGGCCTTGAGTTTGTTGTTCCGGCGTCC
GACGACTAATCTGGGTCGTC

Downstream 100 bases:

>100_bases
GACGGAGGCAGCCTTCGCTGACCCTTAACCACCTGCGTCGGCGGCGCTTTTCCCGCGCCTTTTTTTGAGCGGGAATCGGG
CTAGGATGCGTTAAATAGTC

Product: tRNA(Ile)-lysidine synthase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 367; Mature: 366

Protein sequence:

>367_residues
MAAHETAQANPSDDGRPIGVAEAEVLFASLADAPALVLAVSGGPDSMALLWLAARWRQALADGPRLVAITVDHGLRPEAR
REAAMVKRFARQLEVSHRMLRWTEPKPTTGIPEAARLARYRLLARAARQAGASHVLTAHTRDDQAETVLMRLLRGSGIVG
LAAMAPVSARDGLLLARPLLEISKARLVATLRSAGIEFADDPTNHDPAYTRPRLRALMPALAAEGGDSRTLATLAGRLAR
ANAAIELMVDGAEKHLALRASRGGSIRPAQGQTFEARAFVALPAEIRLRLLMRAINQIGTEGPVELGKAEALLDRMDRTM
DRTLAGVANGGTSAAGRLKQTLAGALVSMTREAIRITPAPPRRPRRS

Sequences:

>Translated_367_residues
MAAHETAQANPSDDGRPIGVAEAEVLFASLADAPALVLAVSGGPDSMALLWLAARWRQALADGPRLVAITVDHGLRPEAR
REAAMVKRFARQLEVSHRMLRWTEPKPTTGIPEAARLARYRLLARAARQAGASHVLTAHTRDDQAETVLMRLLRGSGIVG
LAAMAPVSARDGLLLARPLLEISKARLVATLRSAGIEFADDPTNHDPAYTRPRLRALMPALAAEGGDSRTLATLAGRLAR
ANAAIELMVDGAEKHLALRASRGGSIRPAQGQTFEARAFVALPAEIRLRLLMRAINQIGTEGPVELGKAEALLDRMDRTM
DRTLAGVANGGTSAAGRLKQTLAGALVSMTREAIRITPAPPRRPRRS
>Mature_366_residues
AAHETAQANPSDDGRPIGVAEAEVLFASLADAPALVLAVSGGPDSMALLWLAARWRQALADGPRLVAITVDHGLRPEARR
EAAMVKRFARQLEVSHRMLRWTEPKPTTGIPEAARLARYRLLARAARQAGASHVLTAHTRDDQAETVLMRLLRGSGIVGL
AAMAPVSARDGLLLARPLLEISKARLVATLRSAGIEFADDPTNHDPAYTRPRLRALMPALAAEGGDSRTLATLAGRLARA
NAAIELMVDGAEKHLALRASRGGSIRPAQGQTFEARAFVALPAEIRLRLLMRAINQIGTEGPVELGKAEALLDRMDRTMD
RTLAGVANGGTSAAGRLKQTLAGALVSMTREAIRITPAPPRRPRRS

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=180, Percent_Identity=37.2222222222222, Blast_Score=89, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 39190; Mature: 39059

Theoretical pI: Translated: 11.73; Mature: 11.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAHETAQANPSDDGRPIGVAEAEVLFASLADAPALVLAVSGGPDSMALLWLAARWRQAL
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHH
ADGPRLVAITVDHGLRPEARREAAMVKRFARQLEVSHRMLRWTEPKPTTGIPEAARLARY
CCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
RLLARAARQAGASHVLTAHTRDDQAETVLMRLLRGSGIVGLAAMAPVSARDGLLLARPLL
HHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCCEEHHHHCCCCCCCCCHHHHHHH
EISKARLVATLRSAGIEFADDPTNHDPAYTRPRLRALMPALAAEGGDSRTLATLAGRLAR
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
ANAAIELMVDGAEKHLALRASRGGSIRPAQGQTFEARAFVALPAEIRLRLLMRAINQIGT
CCCEEEEEECCCHHHEEEEECCCCCCCCCCCCCEECEEEEECCHHHHHHHHHHHHHHHCC
EGPVELGKAEALLDRMDRTMDRTLAGVANGGTSAAGRLKQTLAGALVSMTREAIRITPAP
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCC
PRRPRRS
CCCCCCC
>Mature Secondary Structure 
AAHETAQANPSDDGRPIGVAEAEVLFASLADAPALVLAVSGGPDSMALLWLAARWRQAL
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHH
ADGPRLVAITVDHGLRPEARREAAMVKRFARQLEVSHRMLRWTEPKPTTGIPEAARLARY
CCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
RLLARAARQAGASHVLTAHTRDDQAETVLMRLLRGSGIVGLAAMAPVSARDGLLLARPLL
HHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCCEEHHHHCCCCCCCCCHHHHHHH
EISKARLVATLRSAGIEFADDPTNHDPAYTRPRLRALMPALAAEGGDSRTLATLAGRLAR
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
ANAAIELMVDGAEKHLALRASRGGSIRPAQGQTFEARAFVALPAEIRLRLLMRAINQIGT
CCCEEEEEECCCHHHEEEEECCCCCCCCCCCCCEECEEEEECCHHHHHHHHHHHHHHHCC
EGPVELGKAEALLDRMDRTMDRTLAGVANGGTSAAGRLKQTLAGALVSMTREAIRITPAP
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCC
PRRPRRS
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA