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The map label for this gene is ruvC [H]

Identifier: 146338240

GI number: 146338240

Start: 1217482

End: 1218009

Strand: Direct

Name: ruvC [H]

Synonym: BRADO1144

Alternate gene names: 146338240

Gene position: 1217482-1218009 (Clockwise)

Preceding gene: 146338239

Following gene: 146338241

Centisome position: 16.33

GC content: 68.37

Gene sequence:

>528_bases
ATGAAAGCCCAGCCGATTCGCGCAGCTATCCGCATCATCGGGATCGATCCCGGCCTCCGCCGCACCGGCTGGGGCGTGAT
CGAGAGCGAGGGCAACCGGCTGATCTATGTCGGCTGCGGCTCGGTCGAGCCGCCCGACGACCTGCCGCTGGCCAGCCGCC
TGCTGGCGATCCACGAGGGGCTGGCGAAGGTGCTCGCCGATTTTCAGCCGCTGGAGGCCGCGGTTGAGCAGACCTTCGTC
AACAAGGACGGCGTCGCCACCCTGAAGCTCGGCCAAGCCCGCGGCATCGCGATGCTGGCACCGGCGATGTTCGGAATTAC
GGTCGCCGAATACGCCCCCAACCAGGTCAAGAAGACGGTCGTCGGGGCGGGTCACGCCGACAAGGGCCAGATCGCGGTGA
TGCTGAAGATCCTGCTGCCCAAGGCTGAGCCGCCCTCGGCCGACGCGGCCGATGCGCTGGCGATCGCGATCACCCACGCC
CATCACCGCCAGGGCCAGGCGCTGCGCATGAAGGTGGCCGGGCTATGA

Upstream 100 bases:

>100_bases
GAAGATGGGCGGTTGACGGACGGTTAACCATCTGGCCGCATGAGCAGCGGGCTGTTCCGTTTATGTTTCCTCCTGGGCGG
CGCGAGGCTATCTCTAACCC

Downstream 100 bases:

>100_bases
TCGGCAAGCTCAGGGGCCTGATCGATTCCTATGCCGAGGACTTCGTGATCATCGACGTCGGCGGCGTCGGCTATCAGGTG
CACTGCTCGGCGCGCACCCT

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC [H]

Number of amino acids: Translated: 175; Mature: 175

Protein sequence:

>175_residues
MKAQPIRAAIRIIGIDPGLRRTGWGVIESEGNRLIYVGCGSVEPPDDLPLASRLLAIHEGLAKVLADFQPLEAAVEQTFV
NKDGVATLKLGQARGIAMLAPAMFGITVAEYAPNQVKKTVVGAGHADKGQIAVMLKILLPKAEPPSADAADALAIAITHA
HHRQGQALRMKVAGL

Sequences:

>Translated_175_residues
MKAQPIRAAIRIIGIDPGLRRTGWGVIESEGNRLIYVGCGSVEPPDDLPLASRLLAIHEGLAKVLADFQPLEAAVEQTFV
NKDGVATLKLGQARGIAMLAPAMFGITVAEYAPNQVKKTVVGAGHADKGQIAVMLKILLPKAEPPSADAADALAIAITHA
HHRQGQALRMKVAGL
>Mature_175_residues
MKAQPIRAAIRIIGIDPGLRRTGWGVIESEGNRLIYVGCGSVEPPDDLPLASRLLAIHEGLAKVLADFQPLEAAVEQTFV
NKDGVATLKLGQARGIAMLAPAMFGITVAEYAPNQVKKTVVGAGHADKGQIAVMLKILLPKAEPPSADAADALAIAITHA
HHRQGQALRMKVAGL

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family [H]

Homologues:

Organism=Escherichia coli, GI1788170, Length=165, Percent_Identity=49.0909090909091, Blast_Score=120, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176 [H]

Pfam domain/function: PF02075 RuvC [H]

EC number: =3.1.22.4 [H]

Molecular weight: Translated: 18390; Mature: 18390

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAQPIRAAIRIIGIDPGLRRTGWGVIESEGNRLIYVGCGSVEPPDDLPLASRLLAIHEG
CCCCCCEEEEEEEECCCCCCCCCCEEEECCCCEEEEEECCCCCCCCCCCHHHHHHHHHHH
LAKVLADFQPLEAAVEQTFVNKDGVATLKLGQARGIAMLAPAMFGITVAEYAPNQVKKTV
HHHHHHCCCHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHH
VGAGHADKGQIAVMLKILLPKAEPPSADAADALAIAITHAHHRQGQALRMKVAGL
CCCCCCCCCHHEEEEEECCCCCCCCCCCCHHHEEEEEEHHHCCCCCEEEEEECCC
>Mature Secondary Structure
MKAQPIRAAIRIIGIDPGLRRTGWGVIESEGNRLIYVGCGSVEPPDDLPLASRLLAIHEG
CCCCCCEEEEEEEECCCCCCCCCCEEEECCCCEEEEEECCCCCCCCCCCHHHHHHHHHHH
LAKVLADFQPLEAAVEQTFVNKDGVATLKLGQARGIAMLAPAMFGITVAEYAPNQVKKTV
HHHHHHCCCHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHH
VGAGHADKGQIAVMLKILLPKAEPPSADAADALAIAITHAHHRQGQALRMKVAGL
CCCCCCCCCHHEEEEEECCCCCCCCCCCCHHHEEEEEEHHHCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA