Click here to switch to the map view.

The map label for this gene is pgk [H]

Identifier: 146338223

GI number: 146338223

Start: 1197243

End: 1198442

Strand: Reverse

Name: pgk [H]

Synonym: BRADO1122

Alternate gene names: 146338223

Gene position: 1198442-1197243 (Counterclockwise)

Preceding gene: 146338224

Following gene: 146338222

Centisome position: 16.07

GC content: 66.33

Gene sequence:

>1200_bases
GTGGCTTCAATGACCAAATTCCGCACCCTCGATGACGTCGATCCAAAGGGCAAACGCGTGCTGCTGCGCGTCGATCTCAA
CGTGCCCATGGAGAACGGCCACGTGACCGATGCGACGCGCCTGGAGCGCGTTGCGCCGACCATTAATGAGCTTGCGACCA
AGGGCGGCAAGGTCATCCTGCTCGCCCATTTCGGCCGGCCGAAAGGCCGCGACGCCAAGGACTCGCTGAAGCCCGTTGCT
GACGCGCTGTCGAAGGTGATCAAGCGCCCGGTCGCCTTCGCCGATGATTGCATCGGTGAGCCCGCGGCGAAGGCCATTGC
CGCGATGAAGGACGGCGACGTGCTGTGCCTGGAGAACACCCGCTTCCACAAGGAAGAGGAGAAGAACGATCCGGCCTTCG
TCGCAGAACTCGCCAAGCTCGGCGACATCTGGGTCAACGACGCCTTCTCGGCCGCGCACCGCGCCCATGCCTCGACCGAG
GGCCTCGGCCATAAGCTGCCGGCCTATGCCGGCCGCACCATGCAGGCCGAACTCGACGCGCTCGACAAGGCGCTGGGGCG
CCCCGCCAAGCCTGTCATCGCGATCGTCGGCGGCGCCAAGGTGTCGACCAAGATCGACCTGTTGGAAAACCTCGTCACCA
AGGTGGATGCGCTGGTGATCGGTGGCGGCATGGCCAACACCTTCCTGCATGCGCAGGGCGTGCAGATCGGCAAGTCGCTG
GCCGAGAAGGATCTCGCCGCGACCGCGCTGCGGATCATGGAAAAGGCGGAAGCCGCGAACTGCGCGATCATCCTGCCGGT
GGATGCGACCGTGTCCTATCACTTCGCGGCCAACTCGCCGTCGTTCGCCTACGGCCTCGACGCGATCCCGCCTGACAGCA
TGATCCTCGACGTCGGCCCGCAATCGACCGCGCGCATCCATGCCGCGATCGACGACGCCGCGACGCTGGTGTGGAACGGC
CCGCTCGGCGCCTTCGAGCTGAACCCGTTCGACCGCGGCACCATGGTCGCCGCCAAGCACGCGGCGGAACGCACCAAGGC
CAAGAAGCTGATCTCCGTGGCCGGCGGCGGCGATACCGTCGCTGCGCTCAACCAGGCCGGCGTGGCCGGCGATTTCAGCT
ACGTCTCGACCGCTGGCGGCGCGTTCCTGGAATGGATGGAAGGCAAGCCGCTGCCGGGCGTCGAGGTGCTGAAAGCCTGA

Upstream 100 bases:

>100_bases
TGGCTTCCGCCAGGGCATGCGGGTGACGACCAAACAGGAAGGTAATCCGCCGGAGAACACCGACGGATTCAATCACCGCT
GATGAGTCCCTCGTATTACG

Downstream 100 bases:

>100_bases
CGCTCTGACACTTGTCGGCCATGGATGTTTCTGTTATGTTCTATCCATGGCCGAGACCCCGACAGACGCCGTCCATTCGA
TCCTGATCAAGATCCAGGAG

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MASMTKFRTLDDVDPKGKRVLLRVDLNVPMENGHVTDATRLERVAPTINELATKGGKVILLAHFGRPKGRDAKDSLKPVA
DALSKVIKRPVAFADDCIGEPAAKAIAAMKDGDVLCLENTRFHKEEEKNDPAFVAELAKLGDIWVNDAFSAAHRAHASTE
GLGHKLPAYAGRTMQAELDALDKALGRPAKPVIAIVGGAKVSTKIDLLENLVTKVDALVIGGGMANTFLHAQGVQIGKSL
AEKDLAATALRIMEKAEAANCAIILPVDATVSYHFAANSPSFAYGLDAIPPDSMILDVGPQSTARIHAAIDDAATLVWNG
PLGAFELNPFDRGTMVAAKHAAERTKAKKLISVAGGGDTVAALNQAGVAGDFSYVSTAGGAFLEWMEGKPLPGVEVLKA

Sequences:

>Translated_399_residues
MASMTKFRTLDDVDPKGKRVLLRVDLNVPMENGHVTDATRLERVAPTINELATKGGKVILLAHFGRPKGRDAKDSLKPVA
DALSKVIKRPVAFADDCIGEPAAKAIAAMKDGDVLCLENTRFHKEEEKNDPAFVAELAKLGDIWVNDAFSAAHRAHASTE
GLGHKLPAYAGRTMQAELDALDKALGRPAKPVIAIVGGAKVSTKIDLLENLVTKVDALVIGGGMANTFLHAQGVQIGKSL
AEKDLAATALRIMEKAEAANCAIILPVDATVSYHFAANSPSFAYGLDAIPPDSMILDVGPQSTARIHAAIDDAATLVWNG
PLGAFELNPFDRGTMVAAKHAAERTKAKKLISVAGGGDTVAALNQAGVAGDFSYVSTAGGAFLEWMEGKPLPGVEVLKA
>Mature_398_residues
ASMTKFRTLDDVDPKGKRVLLRVDLNVPMENGHVTDATRLERVAPTINELATKGGKVILLAHFGRPKGRDAKDSLKPVAD
ALSKVIKRPVAFADDCIGEPAAKAIAAMKDGDVLCLENTRFHKEEEKNDPAFVAELAKLGDIWVNDAFSAAHRAHASTEG
LGHKLPAYAGRTMQAELDALDKALGRPAKPVIAIVGGAKVSTKIDLLENLVTKVDALVIGGGMANTFLHAQGVQIGKSLA
EKDLAATALRIMEKAEAANCAIILPVDATVSYHFAANSPSFAYGLDAIPPDSMILDVGPQSTARIHAAIDDAATLVWNGP
LGAFELNPFDRGTMVAAKHAAERTKAKKLISVAGGGDTVAALNQAGVAGDFSYVSTAGGAFLEWMEGKPLPGVEVLKA

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI4505763, Length=416, Percent_Identity=42.3076923076923, Blast_Score=289, Evalue=3e-78,
Organism=Homo sapiens, GI31543397, Length=416, Percent_Identity=42.5480769230769, Blast_Score=284, Evalue=1e-76,
Organism=Escherichia coli, GI1789294, Length=392, Percent_Identity=41.5816326530612, Blast_Score=291, Evalue=7e-80,
Organism=Caenorhabditis elegans, GI17508823, Length=416, Percent_Identity=43.9903846153846, Blast_Score=305, Evalue=2e-83,
Organism=Saccharomyces cerevisiae, GI6319857, Length=414, Percent_Identity=39.3719806763285, Blast_Score=265, Evalue=7e-72,
Organism=Drosophila melanogaster, GI24581203, Length=412, Percent_Identity=42.7184466019417, Blast_Score=274, Evalue=7e-74,
Organism=Drosophila melanogaster, GI17136394, Length=410, Percent_Identity=40.9756097560976, Blast_Score=268, Evalue=5e-72,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 41949; Mature: 41817

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASMTKFRTLDDVDPKGKRVLLRVDLNVPMENGHVTDATRLERVAPTINELATKGGKVIL
CCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEE
LAHFGRPKGRDAKDSLKPVADALSKVIKRPVAFADDCIGEPAAKAIAAMKDGDVLCLENT
EEECCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHCCCCCEEEECCC
RFHKEEEKNDPAFVAELAKLGDIWVNDAFSAAHRAHASTEGLGHKLPAYAGRTMQAELDA
CCCCHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCHHHHHHH
LDKALGRPAKPVIAIVGGAKVSTKIDLLENLVTKVDALVIGGGMANTFLHAQGVQIGKSL
HHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHCCHHHHHHH
AEKDLAATALRIMEKAEAANCAIILPVDATVSYHFAANSPSFAYGLDAIPPDSMILDVGP
HHHHHHHHHHHHHHHHCCCCEEEEEEECCEEEEEEECCCCCCEECCCCCCCCCEEEECCC
QSTARIHAAIDDAATLVWNGPLGAFELNPFDRGTMVAAKHAAERTKAKKLISVAGGGDTV
CCCEEEEEEECCCEEEEECCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHEEECCCCCHH
AALNQAGVAGDFSYVSTAGGAFLEWMEGKPLPGVEVLKA
HHHHCCCCCCCCHHHHHCCCHHHHHHCCCCCCCHHHHCC
>Mature Secondary Structure 
ASMTKFRTLDDVDPKGKRVLLRVDLNVPMENGHVTDATRLERVAPTINELATKGGKVIL
CCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEE
LAHFGRPKGRDAKDSLKPVADALSKVIKRPVAFADDCIGEPAAKAIAAMKDGDVLCLENT
EEECCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHCCCCCEEEECCC
RFHKEEEKNDPAFVAELAKLGDIWVNDAFSAAHRAHASTEGLGHKLPAYAGRTMQAELDA
CCCCHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCHHHHHHH
LDKALGRPAKPVIAIVGGAKVSTKIDLLENLVTKVDALVIGGGMANTFLHAQGVQIGKSL
HHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHCCHHHHHHH
AEKDLAATALRIMEKAEAANCAIILPVDATVSYHFAANSPSFAYGLDAIPPDSMILDVGP
HHHHHHHHHHHHHHHHCCCCEEEEEEECCEEEEEEECCCCCCEECCCCCCCCCEEEECCC
QSTARIHAAIDDAATLVWNGPLGAFELNPFDRGTMVAAKHAAERTKAKKLISVAGGGDTV
CCCEEEEEEECCCEEEEECCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHEEECCCCCHH
AALNQAGVAGDFSYVSTAGGAFLEWMEGKPLPGVEVLKA
HHHHCCCCCCCCHHHHHCCCHHHHHHCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]