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The map label for this gene is fadD [H]

Identifier: 146338201

GI number: 146338201

Start: 1170335

End: 1172197

Strand: Reverse

Name: fadD [H]

Synonym: BRADO1099

Alternate gene names: 146338201

Gene position: 1172197-1170335 (Counterclockwise)

Preceding gene: 146338203

Following gene: 146338200

Centisome position: 15.72

GC content: 67.69

Gene sequence:

>1863_bases
ATGGGTAGCCCCGAGCGCCGTCAAGATCCCGCGCCTGAGATGTTCGCCGCGCCATCCATCGACGCGCTGAAGCGTGACGA
CGGCGCCATCATTCTGCGCTCGCGCATGCCGCTGGAGCCTTCTGCGCGCTGTACGGGCGAATGGCTGGAGACGTGGGCGC
GCAAGGCGCCGGACCGCACCTTCCTGGCGGAGCGCCGCGGCGACACCTGGACGTGGCTGACCTACGGCGAGACGCTGACG
CAGGTGCGCGCCGCGGCGGCCTGGATGCTGGCGCAGGGCCTTGGGCCGGAGCGGCCGCTCGCGATCCTCTCCGACAACAG
CATCGCGCATGCGGTGCTGGCGCTCGCCGCGCAGCATGTCGGCATCCCCGTCTGCGCGATCTCGTCGGCCTACTCGCTGA
TGTCGACCGACCATGAGAAGCTCAGGACGATGATCCGGCTGCTCGATCCCGGCGCCATCTTCGTCGCTGCGCACGGCCCC
TTCGTCCGCGCGCTGGCCGCAATCGAAGGCTTGCATCGCGCTGTGATCATCAGCGGCGATGGCGAGGGCTCCGGCGCGAT
GCCACTGCAGGCCTTGCTGACGACGAAGTCCGGTCACGAGGTCGATCGAGCCTTCGCGGCGGTGACGCCCGATACGATCG
CAAAGCTGCTGTTCACCTCCGGCTCGACCGGGCAGCCGAAGGCCGTGATCAACACGCACGGAATGCTGACGATGAGTCAG
CAGGCCAAGGGCCAGCTCTGGACCTTTCTGCGCGAGGTCGAAGGCGGCCCCGTCATCGTCGACTGGCTGCCCTGGAGCCA
CACCTTCGGGGCCAACCACAATTTCAATCTGGTGCTCGCCCATGGCGGCTCGATCTATATCGACGGCGGCAAGCCGGCGC
CCGGCCTGTTCGAGACCTCGCTCGCCAACCTCCGCAGCATGGTGCCGACGATCTATTTCAACGTCCCGCGCGGCTTCGAC
ATGCTGGTCGCGGCGCTCAAGGCAGACGACGAGTTGCGGACAAAGTTCTTCTCCGGCGTGCAGTGCATCCTCTATGCGGC
CGCCGCGCTGCCGCAGAATCTCTGGGATGCGCTCAACGAGATGAGCCTGGCCACGGTCGGCCGGAAGGCGCCGCTGGTGT
CGGCCTGGGGCGCCACGGAAACGTCGCCGCTTGCCACCGACTGCCACTTCTTCGCTGAGCGCGCCGGCAATATCGGCGTA
CCGATCCCCGGCACCGAGCTGAAGCTGGTGCCCGCCGGCGAGAAGCTCGAAGTGCGCGTGCGCGGCCCCAACGTGATGCC
GGGCTACTGGAAGGCGCCGGAGATCACCGCCGCCGCCTTCGACGAGGAAGGTTTCTACAAGATCGGCGACGCCGTGAAGC
TCGCCGACCCCGATCATCCGGAAGCCGGCCTGTTCTTCGACGGCCGCATCACCGAAGATTTCAAACTCTCCTCCGGCACC
TGGGTGAATGTCGGCGCGCTGCGCGTCGCCGGCATTGCTGCGCTGGCGCCGCTGGCACAGGACATCGTCATCGCCGGCCA
CGGCACCGACGAGGTGCATTTCCTGATCGTGCCGAACATCATCGCATGCCGGAGCAAGGCAGCGCTGGCCGACACATGTT
CAATCGGCGAGGTGCTCGCGCATCCTGCCGTGCGCGAGGCGATCGCGCGGGGGCTGGCCAAGCTGCGCGCCGAGGCGGTC
GGCTCGTCGCGCTATGCGACACGCGCGCTGCTGCTGGCCGAGCCGCCGTCGCTCGATGCCGGCGAGATCACCGACAAGGG
CTACATCAATCAGCGCGCCGTTCTCACCCGCCGCGCCAATCTGGTGGCAAGGCTGCAGGACGAGGCTGCGGCGGATCTGA
TCAGGCCAACTCCGAACACCTGA

Upstream 100 bases:

>100_bases
GAGAGAGCGGTGCACGGCACGGTCCGCATCGGCGCGCGGGACGTCGCATCGGCCAACGGCCCTCTTTTTTGCGCAGCCTC
CGACGGGACGGAGGCTTCTC

Downstream 100 bases:

>100_bases
TGACAATGCGGCCGGCAGGGCAAAGCGACTGATGAGGTCACGGGGGCATCGTGGACCATTGCCGCGATCCCTGTTCCGCG
CTGAAATCCCGTAGAACTAC

Product: feruloyl-CoA synthase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 620; Mature: 619

Protein sequence:

>620_residues
MGSPERRQDPAPEMFAAPSIDALKRDDGAIILRSRMPLEPSARCTGEWLETWARKAPDRTFLAERRGDTWTWLTYGETLT
QVRAAAAWMLAQGLGPERPLAILSDNSIAHAVLALAAQHVGIPVCAISSAYSLMSTDHEKLRTMIRLLDPGAIFVAAHGP
FVRALAAIEGLHRAVIISGDGEGSGAMPLQALLTTKSGHEVDRAFAAVTPDTIAKLLFTSGSTGQPKAVINTHGMLTMSQ
QAKGQLWTFLREVEGGPVIVDWLPWSHTFGANHNFNLVLAHGGSIYIDGGKPAPGLFETSLANLRSMVPTIYFNVPRGFD
MLVAALKADDELRTKFFSGVQCILYAAAALPQNLWDALNEMSLATVGRKAPLVSAWGATETSPLATDCHFFAERAGNIGV
PIPGTELKLVPAGEKLEVRVRGPNVMPGYWKAPEITAAAFDEEGFYKIGDAVKLADPDHPEAGLFFDGRITEDFKLSSGT
WVNVGALRVAGIAALAPLAQDIVIAGHGTDEVHFLIVPNIIACRSKAALADTCSIGEVLAHPAVREAIARGLAKLRAEAV
GSSRYATRALLLAEPPSLDAGEITDKGYINQRAVLTRRANLVARLQDEAAADLIRPTPNT

Sequences:

>Translated_620_residues
MGSPERRQDPAPEMFAAPSIDALKRDDGAIILRSRMPLEPSARCTGEWLETWARKAPDRTFLAERRGDTWTWLTYGETLT
QVRAAAAWMLAQGLGPERPLAILSDNSIAHAVLALAAQHVGIPVCAISSAYSLMSTDHEKLRTMIRLLDPGAIFVAAHGP
FVRALAAIEGLHRAVIISGDGEGSGAMPLQALLTTKSGHEVDRAFAAVTPDTIAKLLFTSGSTGQPKAVINTHGMLTMSQ
QAKGQLWTFLREVEGGPVIVDWLPWSHTFGANHNFNLVLAHGGSIYIDGGKPAPGLFETSLANLRSMVPTIYFNVPRGFD
MLVAALKADDELRTKFFSGVQCILYAAAALPQNLWDALNEMSLATVGRKAPLVSAWGATETSPLATDCHFFAERAGNIGV
PIPGTELKLVPAGEKLEVRVRGPNVMPGYWKAPEITAAAFDEEGFYKIGDAVKLADPDHPEAGLFFDGRITEDFKLSSGT
WVNVGALRVAGIAALAPLAQDIVIAGHGTDEVHFLIVPNIIACRSKAALADTCSIGEVLAHPAVREAIARGLAKLRAEAV
GSSRYATRALLLAEPPSLDAGEITDKGYINQRAVLTRRANLVARLQDEAAADLIRPTPNT
>Mature_619_residues
GSPERRQDPAPEMFAAPSIDALKRDDGAIILRSRMPLEPSARCTGEWLETWARKAPDRTFLAERRGDTWTWLTYGETLTQ
VRAAAAWMLAQGLGPERPLAILSDNSIAHAVLALAAQHVGIPVCAISSAYSLMSTDHEKLRTMIRLLDPGAIFVAAHGPF
VRALAAIEGLHRAVIISGDGEGSGAMPLQALLTTKSGHEVDRAFAAVTPDTIAKLLFTSGSTGQPKAVINTHGMLTMSQQ
AKGQLWTFLREVEGGPVIVDWLPWSHTFGANHNFNLVLAHGGSIYIDGGKPAPGLFETSLANLRSMVPTIYFNVPRGFDM
LVAALKADDELRTKFFSGVQCILYAAAALPQNLWDALNEMSLATVGRKAPLVSAWGATETSPLATDCHFFAERAGNIGVP
IPGTELKLVPAGEKLEVRVRGPNVMPGYWKAPEITAAAFDEEGFYKIGDAVKLADPDHPEAGLFFDGRITEDFKLSSGTW
VNVGALRVAGIAALAPLAQDIVIAGHGTDEVHFLIVPNIIACRSKAALADTCSIGEVLAHPAVREAIARGLAKLRAEAVG
SSRYATRALLLAEPPSLDAGEITDKGYINQRAVLTRRANLVARLQDEAAADLIRPTPNT

Specific function: Catalyzes the esterification, concomitant with transport, of exogenous long-chain fatty acids into metabolically active CoA thioesters for subsequent degradation or incorporation into phospholipids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Membrane; Peripheral membrane protein (Potential). Note=Partially membrane-associated (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=416, Percent_Identity=24.7596153846154, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI187761343, Length=416, Percent_Identity=24.7596153846154, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI40807491, Length=400, Percent_Identity=24.75, Blast_Score=74, Evalue=4e-13,
Organism=Escherichia coli, GI1788107, Length=445, Percent_Identity=25.1685393258427, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI145693145, Length=256, Percent_Identity=28.125, Blast_Score=73, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17564090, Length=397, Percent_Identity=27.9596977329975, Blast_Score=90, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17558820, Length=273, Percent_Identity=24.1758241758242, Blast_Score=74, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI32563687, Length=267, Percent_Identity=24.7191011235955, Blast_Score=69, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 66351; Mature: 66220

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSPERRQDPAPEMFAAPSIDALKRDDGAIILRSRMPLEPSARCTGEWLETWARKAPDRT
CCCCCCCCCCCCCCCCCCCHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCH
FLAERRGDTWTWLTYGETLTQVRAAAAWMLAQGLGPERPLAILSDNSIAHAVLALAAQHV
HHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHC
GIPVCAISSAYSLMSTDHEKLRTMIRLLDPGAIFVAAHGPFVRALAAIEGLHRAVIISGD
CCEEEHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHCCCEEEEEECC
GEGSGAMPLQALLTTKSGHEVDRAFAAVTPDTIAKLLFTSGSTGQPKAVINTHGMLTMSQ
CCCCCCCCHHHEEECCCCCHHHHHHHHCCHHHHHHHHHCCCCCCCCCEEEECCCEEEECH
QAKGQLWTFLREVEGGPVIVDWLPWSHTFGANHNFNLVLAHGGSIYIDGGKPAPGLFETS
HHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCEEEEEEECCEEEECCCCCCCCHHHHH
LANLRSMVPTIYFNVPRGFDMLVAALKADDELRTKFFSGVQCILYAAAALPQNLWDALNE
HHHHHHHCCEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
MSLATVGRKAPLVSAWGATETSPLATDCHFFAERAGNIGVPIPGTELKLVPAGEKLEVRV
HHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECCCEEEEEE
RGPNVMPGYWKAPEITAAAFDEEGFYKIGDAVKLADPDHPEAGLFFDGRITEDFKLSSGT
CCCCCCCCCCCCCCEEEEEECCCCCEECCCEEEECCCCCCCCCEEECCCCCCCEECCCCC
WVNVGALRVAGIAALAPLAQDIVIAGHGTDEVHFLIVPNIIACRSKAALADTCSIGEVLA
EEEECHHHHHHHHHHHHHHCCEEEEECCCCCEEEEECCCHHHHCCCCHHHHHCCHHHHHC
HPAVREAIARGLAKLRAEAVGSSRYATRALLLAEPPSLDAGEITDKGYINQRAVLTRRAN
CHHHHHHHHHHHHHHHHHHCCCCCHHEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHH
LVARLQDEAAADLIRPTPNT
HHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
GSPERRQDPAPEMFAAPSIDALKRDDGAIILRSRMPLEPSARCTGEWLETWARKAPDRT
CCCCCCCCCCCCCCCCCCHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCH
FLAERRGDTWTWLTYGETLTQVRAAAAWMLAQGLGPERPLAILSDNSIAHAVLALAAQHV
HHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHC
GIPVCAISSAYSLMSTDHEKLRTMIRLLDPGAIFVAAHGPFVRALAAIEGLHRAVIISGD
CCEEEHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHCCCEEEEEECC
GEGSGAMPLQALLTTKSGHEVDRAFAAVTPDTIAKLLFTSGSTGQPKAVINTHGMLTMSQ
CCCCCCCCHHHEEECCCCCHHHHHHHHCCHHHHHHHHHCCCCCCCCCEEEECCCEEEECH
QAKGQLWTFLREVEGGPVIVDWLPWSHTFGANHNFNLVLAHGGSIYIDGGKPAPGLFETS
HHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCEEEEEEECCEEEECCCCCCCCHHHHH
LANLRSMVPTIYFNVPRGFDMLVAALKADDELRTKFFSGVQCILYAAAALPQNLWDALNE
HHHHHHHCCEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
MSLATVGRKAPLVSAWGATETSPLATDCHFFAERAGNIGVPIPGTELKLVPAGEKLEVRV
HHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECCCEEEEEE
RGPNVMPGYWKAPEITAAAFDEEGFYKIGDAVKLADPDHPEAGLFFDGRITEDFKLSSGT
CCCCCCCCCCCCCCEEEEEECCCCCEECCCEEEECCCCCCCCCEEECCCCCCCEECCCCC
WVNVGALRVAGIAALAPLAQDIVIAGHGTDEVHFLIVPNIIACRSKAALADTCSIGEVLA
EEEECHHHHHHHHHHHHHHCCEEEEECCCCCEEEEECCCHHHHCCCCHHHHHCCHHHHHC
HPAVREAIARGLAKLRAEAVGSSRYATRALLLAEPPSLDAGEITDKGYINQRAVLTRRAN
CHHHHHHHHHHHHHHHHHHCCCCCHHEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHH
LVARLQDEAAADLIRPTPNT
HHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]