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The map label for this gene is pimB [H]

Identifier: 146338087

GI number: 146338087

Start: 1043904

End: 1045091

Strand: Direct

Name: pimB [H]

Synonym: BRADO0983

Alternate gene names: 146338087

Gene position: 1043904-1045091 (Clockwise)

Preceding gene: 146338086

Following gene: 146338088

Centisome position: 14.0

GC content: 68.52

Gene sequence:

>1188_bases
ATGACCGATGCCGTCATCGTTTCCACCGCCCGCACGCCGATCGGCAAGGCCTATCGCGGCGCGCTCAACGCCACCGGCGG
CGCGACGCTGCTCGGCCACGCCATCGGCGAAGCCGTCAAGCGCGCCAAGATCGATCCGGCCGAGGTCGAGGACGTCGTGA
TGGGCGCGGCCCTGCAGCAGGGCTCGACCGGCGGCAACATCGCACGCAAGGCGCTGTTGCGCGCCGGCCTGCCGGTGTCG
GTCGGCGGCACCACCATCGACCGGCAATGCGCTTCGGGTCTGCAGGCGATCGCGCTCGCGGCGCGCTCGGTGATCTTCGA
CGGCGTCGAGATCGCGGTCGGCGGCGGCGGCGAGTCGATCTCGCTGGTCCAGAACGACAAGATGAACAGCTTTCAGGCCA
CCGATGCGGCGCTGCTTGAGATCAAGGGCGACGTCTACATGCCGATGATCGACACCGCCGAGGTGGTCGCCAAGCGCTAC
GGCATCTCGCGCGAGAAGCAGGACGAATATGCGCTGGAGAGCCAGCGCCGCACCGCGGCCGCGCAGCAGGGCGGCAAGTT
CAACGAGGAACTGGCGCCGATCTCGACCAAGATGGCGGTGACAGACAAGGCGACCGGCAACATCTCGTTCAAGGACATCA
CGCTGTCGCAAGATGAGGGCCCGCGCCCGGAGACCACCGCCGAAGGTCTCGCCGGCCTCAAGGCCGTGCGCGGCGACGGC
TTCACCATCACCGCCGGCAATGCCAGCCAGCTGTCGGACGGCGCCTCGGCCTCGGTGATCATGAGCGACAAGGAAGCCGC
CAAGCGCGGCCTGCAGCCGCTCGGCATCTTCCGCGGCTTCGTCGCGCATGGCTGCGAGCCGGACGAGATGGGCATCGGCC
CGGTGTTCGCGGTGCCGCGCCTGCTGAAGCGCCACGGCCTCACCGTCGACGACATCGGCCTGTGGGAGCTCAACGAGGCC
TTCGCAGTGCAAGTGTTGTATTGCCGCGACAAGCTCGGCATCGACCCCGACAAGATCAACGTCAATGGCGGCGCCATCTC
GGTCGGCCATCCCTACGGCATGTCGGGCGCGCGCCTCACCGGCCACGCCCTGATCGAGGGCCGCCGCCGCAAGGCCAAAT
ACGCCGTCGTCACCATGTGCGTCGGCGGCGGCATGGGCGCCGCCGGCCTATTCGAGGTGCTGCAATAA

Upstream 100 bases:

>100_bases
CCGCGCACGCCGGTCGGCAAATTGTCGCGTCACGAATTACGCCAGCAGCAATCATCAACATCTCACAGCAAACAATCGTA
ACACCAACAGGAGGTCGCCC

Downstream 100 bases:

>100_bases
CAAAGACAGCTGTCGTCCCGGGCGAAGCTCGCGCGGGATTTCAAACAATTGTCGTCCCCGGGCCAAGGTCGCGCGGGATT
CAAAACAGGTGTCGTCCCGG

Product: acetyl-CoA acetyltransferase

Products: NA

Alternate protein names: Acetyl-CoA acyltransferase; Beta-ketothiolase [H]

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MTDAVIVSTARTPIGKAYRGALNATGGATLLGHAIGEAVKRAKIDPAEVEDVVMGAALQQGSTGGNIARKALLRAGLPVS
VGGTTIDRQCASGLQAIALAARSVIFDGVEIAVGGGGESISLVQNDKMNSFQATDAALLEIKGDVYMPMIDTAEVVAKRY
GISREKQDEYALESQRRTAAAQQGGKFNEELAPISTKMAVTDKATGNISFKDITLSQDEGPRPETTAEGLAGLKAVRGDG
FTITAGNASQLSDGASASVIMSDKEAAKRGLQPLGIFRGFVAHGCEPDEMGIGPVFAVPRLLKRHGLTVDDIGLWELNEA
FAVQVLYCRDKLGIDPDKINVNGGAISVGHPYGMSGARLTGHALIEGRRRKAKYAVVTMCVGGGMGAAGLFEVLQ

Sequences:

>Translated_395_residues
MTDAVIVSTARTPIGKAYRGALNATGGATLLGHAIGEAVKRAKIDPAEVEDVVMGAALQQGSTGGNIARKALLRAGLPVS
VGGTTIDRQCASGLQAIALAARSVIFDGVEIAVGGGGESISLVQNDKMNSFQATDAALLEIKGDVYMPMIDTAEVVAKRY
GISREKQDEYALESQRRTAAAQQGGKFNEELAPISTKMAVTDKATGNISFKDITLSQDEGPRPETTAEGLAGLKAVRGDG
FTITAGNASQLSDGASASVIMSDKEAAKRGLQPLGIFRGFVAHGCEPDEMGIGPVFAVPRLLKRHGLTVDDIGLWELNEA
FAVQVLYCRDKLGIDPDKINVNGGAISVGHPYGMSGARLTGHALIEGRRRKAKYAVVTMCVGGGMGAAGLFEVLQ
>Mature_394_residues
TDAVIVSTARTPIGKAYRGALNATGGATLLGHAIGEAVKRAKIDPAEVEDVVMGAALQQGSTGGNIARKALLRAGLPVSV
GGTTIDRQCASGLQAIALAARSVIFDGVEIAVGGGGESISLVQNDKMNSFQATDAALLEIKGDVYMPMIDTAEVVAKRYG
ISREKQDEYALESQRRTAAAQQGGKFNEELAPISTKMAVTDKATGNISFKDITLSQDEGPRPETTAEGLAGLKAVRGDGF
TITAGNASQLSDGASASVIMSDKEAAKRGLQPLGIFRGFVAHGCEPDEMGIGPVFAVPRLLKRHGLTVDDIGLWELNEAF
AVQVLYCRDKLGIDPDKINVNGGAISVGHPYGMSGARLTGHALIEGRRRKAKYAVVTMCVGGGMGAAGLFEVLQ

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4501853, Length=393, Percent_Identity=43.765903307888, Blast_Score=283, Evalue=3e-76,
Organism=Homo sapiens, GI167614485, Length=397, Percent_Identity=40.3022670025189, Blast_Score=256, Evalue=2e-68,
Organism=Homo sapiens, GI4557237, Length=403, Percent_Identity=37.9652605459057, Blast_Score=229, Evalue=2e-60,
Organism=Homo sapiens, GI148539872, Length=395, Percent_Identity=38.2278481012658, Blast_Score=223, Evalue=2e-58,
Organism=Homo sapiens, GI4504327, Length=442, Percent_Identity=28.2805429864253, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI194353979, Length=245, Percent_Identity=37.1428571428571, Blast_Score=119, Evalue=4e-27,
Organism=Escherichia coli, GI1787663, Length=412, Percent_Identity=41.747572815534, Blast_Score=253, Evalue=2e-68,
Organism=Escherichia coli, GI1788554, Length=407, Percent_Identity=41.7690417690418, Blast_Score=250, Evalue=1e-67,
Organism=Escherichia coli, GI87082165, Length=402, Percent_Identity=36.5671641791045, Blast_Score=230, Evalue=1e-61,
Organism=Escherichia coli, GI48994986, Length=405, Percent_Identity=37.2839506172839, Blast_Score=221, Evalue=6e-59,
Organism=Escherichia coli, GI1788683, Length=420, Percent_Identity=31.6666666666667, Blast_Score=165, Evalue=5e-42,
Organism=Caenorhabditis elegans, GI133906874, Length=397, Percent_Identity=36.5239294710327, Blast_Score=218, Evalue=4e-57,
Organism=Caenorhabditis elegans, GI25147385, Length=395, Percent_Identity=34.4303797468354, Blast_Score=214, Evalue=5e-56,
Organism=Caenorhabditis elegans, GI17535921, Length=407, Percent_Identity=36.6093366093366, Blast_Score=206, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI17551802, Length=441, Percent_Identity=29.2517006802721, Blast_Score=162, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17535917, Length=390, Percent_Identity=28.2051282051282, Blast_Score=139, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6322031, Length=399, Percent_Identity=37.34335839599, Blast_Score=226, Evalue=5e-60,
Organism=Saccharomyces cerevisiae, GI6325229, Length=402, Percent_Identity=30.8457711442786, Blast_Score=180, Evalue=3e-46,
Organism=Drosophila melanogaster, GI24655093, Length=405, Percent_Identity=40, Blast_Score=255, Evalue=4e-68,
Organism=Drosophila melanogaster, GI17648125, Length=399, Percent_Identity=37.844611528822, Blast_Score=236, Evalue=2e-62,
Organism=Drosophila melanogaster, GI24640423, Length=404, Percent_Identity=36.6336633663366, Blast_Score=234, Evalue=7e-62,
Organism=Drosophila melanogaster, GI17137578, Length=438, Percent_Identity=30.1369863013699, Blast_Score=173, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.16 [H]

Molecular weight: Translated: 41068; Mature: 40937

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDAVIVSTARTPIGKAYRGALNATGGATLLGHAIGEAVKRAKIDPAEVEDVVMGAALQQ
CCCEEEEECCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHC
GSTGGNIARKALLRAGLPVSVGGTTIDRQCASGLQAIALAARSVIFDGVEIAVGGGGESI
CCCCHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEE
SLVQNDKMNSFQATDAALLEIKGDVYMPMIDTAEVVAKRYGISREKQDEYALESQRRTAA
EEEECCCCCCCCCCCEEEEEECCCEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
AQQGGKFNEELAPISTKMAVTDKATGNISFKDITLSQDEGPRPETTAEGLAGLKAVRGDG
HHCCCCCCCHHCCCEEEEEEECCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCC
FTITAGNASQLSDGASASVIMSDKEAAKRGLQPLGIFRGFVAHGCEPDEMGIGPVFAVPR
EEEECCCHHHCCCCCCEEEEECCHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHH
LLKRHGLTVDDIGLWELNEAFAVQVLYCRDKLGIDPDKINVNGGAISVGHPYGMSGARLT
HHHHCCCCEECCCCEECCHHHEEEEEEEHHHCCCCCCEEEECCCEEECCCCCCCCCCCEE
GHALIEGRRRKAKYAVVTMCVGGGMGAAGLFEVLQ
HHHHHHCHHHCCEEEEEEEEECCCCCHHHHHHHHC
>Mature Secondary Structure 
TDAVIVSTARTPIGKAYRGALNATGGATLLGHAIGEAVKRAKIDPAEVEDVVMGAALQQ
CCEEEEECCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHC
GSTGGNIARKALLRAGLPVSVGGTTIDRQCASGLQAIALAARSVIFDGVEIAVGGGGESI
CCCCHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEE
SLVQNDKMNSFQATDAALLEIKGDVYMPMIDTAEVVAKRYGISREKQDEYALESQRRTAA
EEEECCCCCCCCCCCEEEEEECCCEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
AQQGGKFNEELAPISTKMAVTDKATGNISFKDITLSQDEGPRPETTAEGLAGLKAVRGDG
HHCCCCCCCHHCCCEEEEEEECCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCC
FTITAGNASQLSDGASASVIMSDKEAAKRGLQPLGIFRGFVAHGCEPDEMGIGPVFAVPR
EEEECCCHHHCCCCCCEEEEECCHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHH
LLKRHGLTVDDIGLWELNEAFAVQVLYCRDKLGIDPDKINVNGGAISVGHPYGMSGARLT
HHHHCCCCEECCCCEECCHHHEEEEEEEHHHCCCCCCEEEECCCEEECCCCCCCCCCCEE
GHALIEGRRRKAKYAVVTMCVGGGMGAAGLFEVLQ
HHHHHHCHHHCCEEEEEEEEECCCCCHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]