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The map label for this gene is livM [H]

Identifier: 146338080

GI number: 146338080

Start: 1035351

End: 1036367

Strand: Reverse

Name: livM [H]

Synonym: BRADO0976

Alternate gene names: 146338080

Gene position: 1036367-1035351 (Counterclockwise)

Preceding gene: 146338081

Following gene: 146338079

Centisome position: 13.9

GC content: 63.42

Gene sequence:

>1017_bases
ATGAGCGCAGTCGAGGAAATCGCATCCCAGGCGCCCGCGGTCGAGGCCGTTCCGAAGCGGGCCATGACACTGAGCTCGGG
CACAACCTTCGTCGTCGTGCTTCTGCTTCTAATCGTTCCGCTGTTCGTCAAGAACTTCATCATCTTCCAGATGACGATGC
TCCTGATCTATGGCCTGGCCGTGCTGGCGCTGAACATCCTGACCGGCGGCTCCGGACAGTTCTCGCTTGGGCAGAGCGCG
TTCTACGCCGTCGGCGCTTACACGTCTGCGATCCTGATGGAGCAATACAACGTCAACTATGCGCTGACCCTGCCGATCGC
GGGCGTCATCTGCTTCGGCTTCGGCTTCCTGTTCGGACAGCCGGCGCTGCGGCTGTCCGGCGTCTATCTCGCGCTCGCCA
CCTTCGCACTCGCCACAGCGATGCCCCAGATCCTCAAGCTCGGCTTCTTCGAGCACTGGACCGGCGGCGTGCAGGGCCTC
GTCGTCACCAAGCCCGATGCGCCGTTCGGCCTGCCGATGTCGCAAGATATGTGGCTTTACTACTTTACGCTCGCGATCAC
GATCGCGATCTACATCTTCTCGGTGAACCTGCTGCGCTCGCGCTCCGGCCGCGCCTTCATGGCGATCCGTGACAATGAGA
TCGCCGCCTCAGCCATGGGCATCGACGTGGCGACCTACAAGACGCTGGCGTTCGGCGTCAGTGCCGGCATCACCGGCGTG
GCCGGTGGGCTCGGTGCCATCGCGGTGCAGTTCGTCGCGCCCGACAGCTACACGATCGTGCTCGCGATCTCGCTGTTCCT
CGGCATGGTCGTCGGCGGCGTTGGCTGGCTGCCCGGCTCTTTCGTGGGCGCCGCCTTCATCATTTTCGTCCCCAATATCG
CCGAAGGCATCTCCAAGGGCCTGTCGGGCGCGGTGTTCGGCGTGCTGCTGTTCCTCGTCATCTTCCTCGTGCCGCACGGT
TCGCGGCAGGTGGCGATGGTGGCCCAGCAGCTGCTCGGCAAACTCAGGAAGAACTAA

Upstream 100 bases:

>100_bases
ATGAGATGAAGCTGCCGATCGCGCTGGCGCTGATCATCTCGGTCCTGGTCATCAAACCCGCCGGCCTGTTCGGTCGCAAC
ATCGTGAAGCGAGTCTGATC

Downstream 100 bases:

>100_bases
GGAGGAATTCTTGCAGGTCGTTCAACAACTACGTATCGCCGCCCTCGCGACGGCGGTCATCGCCCTCACGTCGGGCGCGG
CACTCGCCCAGAAGAAGTAT

Product: high-affinity branched-chain amino acid ABC transporter permease

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: LIV-I protein M [H]

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MSAVEEIASQAPAVEAVPKRAMTLSSGTTFVVVLLLLIVPLFVKNFIIFQMTMLLIYGLAVLALNILTGGSGQFSLGQSA
FYAVGAYTSAILMEQYNVNYALTLPIAGVICFGFGFLFGQPALRLSGVYLALATFALATAMPQILKLGFFEHWTGGVQGL
VVTKPDAPFGLPMSQDMWLYYFTLAITIAIYIFSVNLLRSRSGRAFMAIRDNEIAASAMGIDVATYKTLAFGVSAGITGV
AGGLGAIAVQFVAPDSYTIVLAISLFLGMVVGGVGWLPGSFVGAAFIIFVPNIAEGISKGLSGAVFGVLLFLVIFLVPHG
SRQVAMVAQQLLGKLRKN

Sequences:

>Translated_338_residues
MSAVEEIASQAPAVEAVPKRAMTLSSGTTFVVVLLLLIVPLFVKNFIIFQMTMLLIYGLAVLALNILTGGSGQFSLGQSA
FYAVGAYTSAILMEQYNVNYALTLPIAGVICFGFGFLFGQPALRLSGVYLALATFALATAMPQILKLGFFEHWTGGVQGL
VVTKPDAPFGLPMSQDMWLYYFTLAITIAIYIFSVNLLRSRSGRAFMAIRDNEIAASAMGIDVATYKTLAFGVSAGITGV
AGGLGAIAVQFVAPDSYTIVLAISLFLGMVVGGVGWLPGSFVGAAFIIFVPNIAEGISKGLSGAVFGVLLFLVIFLVPHG
SRQVAMVAQQLLGKLRKN
>Mature_337_residues
SAVEEIASQAPAVEAVPKRAMTLSSGTTFVVVLLLLIVPLFVKNFIIFQMTMLLIYGLAVLALNILTGGSGQFSLGQSAF
YAVGAYTSAILMEQYNVNYALTLPIAGVICFGFGFLFGQPALRLSGVYLALATFALATAMPQILKLGFFEHWTGGVQGLV
VTKPDAPFGLPMSQDMWLYYFTLAITIAIYIFSVNLLRSRSGRAFMAIRDNEIAASAMGIDVATYKTLAFGVSAGITGVA
GGLGAIAVQFVAPDSYTIVLAISLFLGMVVGGVGWLPGSFVGAAFIIFVPNIAEGISKGLSGAVFGVLLFLVIFLVPHGS
RQVAMVAQQLLGKLRKN

Specific function: Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=259, Percent_Identity=33.976833976834, Blast_Score=105, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 35681; Mature: 35550

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAVEEIASQAPAVEAVPKRAMTLSSGTTFVVVLLLLIVPLFVKNFIIFQMTMLLIYGLA
CCHHHHHHHCCCCHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLALNILTGGSGQFSLGQSAFYAVGAYTSAILMEQYNVNYALTLPIAGVICFGFGFLFGQ
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHCC
PALRLSGVYLALATFALATAMPQILKLGFFEHWTGGVQGLVVTKPDAPFGLPMSQDMWLY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHH
YFTLAITIAIYIFSVNLLRSRSGRAFMAIRDNEIAASAMGIDVATYKTLAFGVSAGITGV
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHCCHHHHHHHHHHHHHCCCHHH
AGGLGAIAVQFVAPDSYTIVLAISLFLGMVVGGVGWLPGSFVGAAFIIFVPNIAEGISKG
HCCHHHHEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHC
LSGAVFGVLLFLVIFLVPHGSRQVAMVAQQLLGKLRKN
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SAVEEIASQAPAVEAVPKRAMTLSSGTTFVVVLLLLIVPLFVKNFIIFQMTMLLIYGLA
CHHHHHHHCCCCHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLALNILTGGSGQFSLGQSAFYAVGAYTSAILMEQYNVNYALTLPIAGVICFGFGFLFGQ
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHCC
PALRLSGVYLALATFALATAMPQILKLGFFEHWTGGVQGLVVTKPDAPFGLPMSQDMWLY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHH
YFTLAITIAIYIFSVNLLRSRSGRAFMAIRDNEIAASAMGIDVATYKTLAFGVSAGITGV
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHCCHHHHHHHHHHHHHCCCHHH
AGGLGAIAVQFVAPDSYTIVLAISLFLGMVVGGVGWLPGSFVGAAFIIFVPNIAEGISKG
HCCHHHHEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHC
LSGAVFGVLLFLVIFLVPHGSRQVAMVAQQLLGKLRKN
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2195019; 8041620; 9278503 [H]