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The map label for this gene is livK [C]

Identifier: 146338079

GI number: 146338079

Start: 1034117

End: 1035340

Strand: Reverse

Name: livK [C]

Synonym: BRADO0975

Alternate gene names: 146338079

Gene position: 1035340-1034117 (Counterclockwise)

Preceding gene: 146338080

Following gene: 146338078

Centisome position: 13.88

GC content: 62.17

Gene sequence:

>1224_bases
TTGCAGGTCGTTCAACAACTACGTATCGCCGCCCTCGCGACGGCGGTCATCGCCCTCACGTCGGGCGCGGCACTCGCCCA
GAAGAAGTATGACACCGGCGCATCCGATACCGAGATCAAGATCGGCAACATCATGCCCTACAGCGGACCCGCGTCTGCCT
ACGGCGTGATCGGAAAGACCGAGGAGGCCTATTTCAAGAAGATCAACGCCGAGGGCGGCATCAACGGCCGCAAGATCAAC
TTCGTCACCTACGATGACGCCTACTCGCCGCCGAAGGCCGTGGAGCAGGTTCGCAAGCTGGTCGAGAGCGATGAGGTCCT
GGTCGTCTTCAACCCGCTGGGCACGCCGTCGAACTCGGCGATCCAGAAATATCTCAACGGCAAGAAGATCCCGCAGCTGT
TCGTCGCGACCGGCGCCACCAAGTGGAACGATCCCAAGAACTTCCCCTGGACCATGGGCTGGCAGCCGAGCTACCAGAGC
GAGGGCCGTATCTATGCGAAGTACCTGCTCAAGGAGAAGCCCGGCGCCAAGATCGGCGTGCTGTTCCAGAACGACGATTT
CGGCAAGGACTATCTGAAGGGCCTCAAGGACGGTCTCGGCGACAAGGCTGCGTCGATGATCGTGATGGAGGAGAGCTACG
AGACCTCCGAACCGTCGATCGACGGCCATATCGTCAAGCTGAAGGCTTCCGGCGCAGATACCTTCTTCAGCGTCACCACG
CCGAAATTCGCGGCGCAGGCGATCAAGAAGCTCGCCGAGATCGACTGGCATCCGCTGCATCTGGTGGTGAACGTCTCGGC
TTCGGTCGGCAGCGTGATCAAGCCGGCGGGCTTCGAGAACGCGCAGGGCATTCTCTCGGCCGCCTACGCCAAGGACGCCG
CCGATGCGCAGTGGGACAATGATCCCGGCATGAAGAAGTTCTTCGACTTCCTCGCGCAGTACGCGCCCGATGCCAACAAG
CTCGACAGCTCGACCGTCTATGGCTACGGCGCCGCGCAGACGCTGGTGAAGGTGCTGCAGATGTGCGGCGACGACCTCAC
CCGCGCCAACGTCATGAAGCAGGCCGCGAGCCTGAAGGATTTCGCGCCGGACACGCTGCTGCCCGGCGTCAAGATCAACA
CCTCGGCCACCGACTTCGCCCCGATCGAGCAGCTGCAGATGATGCGGTTCAAGGGCGAGAAGTGGGAGCTGTTCGGCGAG
GCCATCAGCGGCGCCGTCGAGTAA

Upstream 100 bases:

>100_bases
CTGTTCCTCGTCATCTTCCTCGTGCCGCACGGTTCGCGGCAGGTGGCGATGGTGGCCCAGCAGCTGCTCGGCAAACTCAG
GAAGAACTAAGGAGGAATTC

Downstream 100 bases:

>100_bases
CCGAAGAAGCGAGCCATCGTGCATCGCCGCAGAAAGCGATGCCGATCTACACTGGCCCTCCGTGCTACCGGCACGGAGGG
CTTTTTCATGCGCGCAGCTT

Product: branched chain amino acid ABC transporter periplasmic-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: None

Number of amino acids: Translated: 407; Mature: 407

Protein sequence:

>407_residues
MQVVQQLRIAALATAVIALTSGAALAQKKYDTGASDTEIKIGNIMPYSGPASAYGVIGKTEEAYFKKINAEGGINGRKIN
FVTYDDAYSPPKAVEQVRKLVESDEVLVVFNPLGTPSNSAIQKYLNGKKIPQLFVATGATKWNDPKNFPWTMGWQPSYQS
EGRIYAKYLLKEKPGAKIGVLFQNDDFGKDYLKGLKDGLGDKAASMIVMEESYETSEPSIDGHIVKLKASGADTFFSVTT
PKFAAQAIKKLAEIDWHPLHLVVNVSASVGSVIKPAGFENAQGILSAAYAKDAADAQWDNDPGMKKFFDFLAQYAPDANK
LDSSTVYGYGAAQTLVKVLQMCGDDLTRANVMKQAASLKDFAPDTLLPGVKINTSATDFAPIEQLQMMRFKGEKWELFGE
AISGAVE

Sequences:

>Translated_407_residues
MQVVQQLRIAALATAVIALTSGAALAQKKYDTGASDTEIKIGNIMPYSGPASAYGVIGKTEEAYFKKINAEGGINGRKIN
FVTYDDAYSPPKAVEQVRKLVESDEVLVVFNPLGTPSNSAIQKYLNGKKIPQLFVATGATKWNDPKNFPWTMGWQPSYQS
EGRIYAKYLLKEKPGAKIGVLFQNDDFGKDYLKGLKDGLGDKAASMIVMEESYETSEPSIDGHIVKLKASGADTFFSVTT
PKFAAQAIKKLAEIDWHPLHLVVNVSASVGSVIKPAGFENAQGILSAAYAKDAADAQWDNDPGMKKFFDFLAQYAPDANK
LDSSTVYGYGAAQTLVKVLQMCGDDLTRANVMKQAASLKDFAPDTLLPGVKINTSATDFAPIEQLQMMRFKGEKWELFGE
AISGAVE
>Mature_407_residues
MQVVQQLRIAALATAVIALTSGAALAQKKYDTGASDTEIKIGNIMPYSGPASAYGVIGKTEEAYFKKINAEGGINGRKIN
FVTYDDAYSPPKAVEQVRKLVESDEVLVVFNPLGTPSNSAIQKYLNGKKIPQLFVATGATKWNDPKNFPWTMGWQPSYQS
EGRIYAKYLLKEKPGAKIGVLFQNDDFGKDYLKGLKDGLGDKAASMIVMEESYETSEPSIDGHIVKLKASGADTFFSVTT
PKFAAQAIKKLAEIDWHPLHLVVNVSASVGSVIKPAGFENAQGILSAAYAKDAADAQWDNDPGMKKFFDFLAQYAPDANK
LDSSTVYGYGAAQTLVKVLQMCGDDLTRANVMKQAASLKDFAPDTLLPGVKINTSATDFAPIEQLQMMRFKGEKWELFGE
AISGAVE

Specific function: This Protein Is A Component Of The Leucine-Specific Transport System, Which Is One Of The Two Periplasmic Binding Protein-Dependent Transport Systems Of The High-Affinity Transport Of The Branched-Chain Amino Acids In E.Coli. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789867, Length=378, Percent_Identity=24.3386243386243, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 44117; Mature: 44117

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVVQQLRIAALATAVIALTSGAALAQKKYDTGASDTEIKIGNIMPYSGPASAYGVIGKT
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCC
EEAYFKKINAEGGINGRKINFVTYDDAYSPPKAVEQVRKLVESDEVLVVFNPLGTPSNSA
HHHHHHHHCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCHHH
IQKYLNGKKIPQLFVATGATKWNDPKNFPWTMGWQPSYQSEGRIYAKYLLKEKPGAKIGV
HHHHHCCCCCCEEEEEECCCCCCCCCCCCEECCCCCCCCCCCCEEEEEHHHCCCCCEEEE
LFQNDDFGKDYLKGLKDGLGDKAASMIVMEESYETSEPSIDGHIVKLKASGADTFFSVTT
EEECCCCCHHHHHHHHHCCCCHHHEEEEEECCCCCCCCCCCCEEEEEEECCCCCEEEECC
PKFAAQAIKKLAEIDWHPLHLVVNVSASVGSVIKPAGFENAQGILSAAYAKDAADAQWDN
HHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHCCCCCCCHHHHHHHHHHCCCCCCCCCC
DPGMKKFFDFLAQYAPDANKLDSSTVYGYGAAQTLVKVLQMCGDDLTRANVMKQAASLKD
CCCHHHHHHHHHHHCCCCCCCCCCEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
FAPDTLLPGVKINTSATDFAPIEQLQMMRFKGEKWELFGEAISGAVE
CCCCHHCCCCEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MQVVQQLRIAALATAVIALTSGAALAQKKYDTGASDTEIKIGNIMPYSGPASAYGVIGKT
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCC
EEAYFKKINAEGGINGRKINFVTYDDAYSPPKAVEQVRKLVESDEVLVVFNPLGTPSNSA
HHHHHHHHCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCHHH
IQKYLNGKKIPQLFVATGATKWNDPKNFPWTMGWQPSYQSEGRIYAKYLLKEKPGAKIGV
HHHHHCCCCCCEEEEEECCCCCCCCCCCCEECCCCCCCCCCCCEEEEEHHHCCCCCEEEE
LFQNDDFGKDYLKGLKDGLGDKAASMIVMEESYETSEPSIDGHIVKLKASGADTFFSVTT
EEECCCCCHHHHHHHHHCCCCHHHEEEEEECCCCCCCCCCCCEEEEEEECCCCCEEEECC
PKFAAQAIKKLAEIDWHPLHLVVNVSASVGSVIKPAGFENAQGILSAAYAKDAADAQWDN
HHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHCCCCCCCHHHHHHHHHHCCCCCCCCCC
DPGMKKFFDFLAQYAPDANKLDSSTVYGYGAAQTLVKVLQMCGDDLTRANVMKQAASLKD
CCCHHHHHHHHHHHCCCCCCCCCCEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
FAPDTLLPGVKINTSATDFAPIEQLQMMRFKGEKWELFGEAISGAVE
CCCCHHCCCCEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA