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The map label for this gene is gsiB [H]

Identifier: 146338010

GI number: 146338010

Start: 963205

End: 964878

Strand: Direct

Name: gsiB [H]

Synonym: BRADO0903

Alternate gene names: 146338010

Gene position: 963205-964878 (Clockwise)

Preceding gene: 146338008

Following gene: 146338011

Centisome position: 12.92

GC content: 65.95

Gene sequence:

>1674_bases
GTGCTTTTGCGTCTTGCGCCCTCAGTGACGAAGCGCGCGGCCATTGGGGCCGCGATGATGACGAGCGCCGCCATCCTCAT
GCTGCCGGCGACGGCCGGCGCGGAGAGCGTGCTGCGCATCGGCATGACCGCGGCGGACATTCCGCGCACGCTCGGCCAGC
CCGACCAGGGTTTCGAGGGCAACCGCTTCACCGGTCTCACGATGTACGACGCGCTCACGATGTGGGACCTGTCGTCGGCG
GACAAGCCGAGCGTCGTCATTCCCGGCCTCGCGACCGAGTGGAAGGTTGACGAGTCCGACAAGACCAAATGGACCTTCAA
GCTGCGTCCCGGCGTCACCTTCCATGATGGCGCGCCGTTCAATGCCGACGCCGTGGTGTGGAACGTCGAGAAGGTGCTCA
AGCAGGACGCGCCGCAATTCGATCCGAGTCAGGTCGGCGTCACGGCCTCGCGCATGCCGACGCTGGCGTCGGCGCGCAAG
ATCGACGATCTCACCGTCGAGCTCGTGACCAAGGAGCCGGACAGTTTCCTGCCGATCAATCTCACCAACCTGTTCATGGC
GAGCCCGGCGAAGTGGCAGAAGCTCTATGAATCCGCGACCGGCGCCGATGACAAGGCGAAGTCGCAGGCCGCGTGGGCGG
CGTTCGCCAAGGACGCCTCGGGCACCGGCCCGTGGAAGATGAGCAAGTTCACGCCGCGCGAGCGGCTGGAGCTCGTCAAG
AACGAGGCCTATTGGGACAAGGCGCGCGTGCCCAAGGCCGACAAGATGATCCTGCTGCCGATGCCCGAAGCCAATGCGCG
CACCGCGGCCCTGCTGTCGGGACAGGTCGACTGGGTCGAGGCGCCGGCGCCGGATGCGCTGCCGGAGATCAAGCAGCGCG
GCTTCAAGCTCTACGCCAATGAGCAGCCGCATGTCTGGCCCTGGCAGTTCTCGCGCGTCGAGGGCTCGCCCTGGAACGAC
ATCCGCGTGCGCAAGGCCGCCAATCTCTGCATCGATCGCGAGGGGCTGCGCGACGGTCTGCTGGCCGGACTGATGGTGCC
GGCGACCGGCACCTTCGAGAAGGGCCATCCCTGGCGCGGCAACGTCACCTTCGAGATCAAGTATGACAAGGCCGCCGCGC
AGAAGCTGATGCAGGAGGCCGGCTACGGCCCGAACAAGAAGCTCGAGGTGAAGACGCAGACCTCGGCCTCCGGCTCGGGC
CAGATGCAGCCGCTGCCGATGAACGAATATCTGCAGCAGGCGCTGGCGGAGTGCTATTTCGACGTCAAGCTCGATGTCAT
CGAGTGGAACACGCTGTTCACCAACTGGCGCCGCGGCGCCAAGGATGCGTCGGCGAACGGCGCCAATGCCACCAACGTCA
CCTATGCGGCGATGGACCCGTTCTTCGCGCTGGTGCGCTTCCTGCAGTCGTCGATGGCGCCGCCGGTGTCGAACAATTGG
GGCTTCATCAACAATCCGAAGTTCGATGAGCTGGTGAAGAAGGCGCGCCAGACCTTCGACCCCGCCGAGCGCGACAAGGC
GCTGGCCGAGCTGCATGCCGCGTCGGTGGATGACGCCGCGTTCCTCTACGTCGCCCACGACGTCGGCCCGCGCGCGATGA
GCCCGAAGGTGAAGGGCGTGGTGCAGCCGAAGAGCTGGTTCATCGACTTCTCGCCGATCTCGGTGGAGCCGTAA

Upstream 100 bases:

>100_bases
TGTGACCACGCGTCGCAGGCGTGCGATCGGGAGCATCCCTGGCATAGGCGTTGCATACTGCTGCCCAGAATTTAGCCAGC
CGATATCGGAGATCTTGATC

Downstream 100 bases:

>100_bases
GGCTCCATCCTCCACTCGTCGTCATGGCCGGGCTTGACCCGGCCATCCATCTTTGACCCGTGGAGGAGACCGCGTGGATG
CCCGGGTCAAGCCCGGGCAT

Product: putative oligopeptide ABC transporter substrate binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 557; Mature: 557

Protein sequence:

>557_residues
MLLRLAPSVTKRAAIGAAMMTSAAILMLPATAGAESVLRIGMTAADIPRTLGQPDQGFEGNRFTGLTMYDALTMWDLSSA
DKPSVVIPGLATEWKVDESDKTKWTFKLRPGVTFHDGAPFNADAVVWNVEKVLKQDAPQFDPSQVGVTASRMPTLASARK
IDDLTVELVTKEPDSFLPINLTNLFMASPAKWQKLYESATGADDKAKSQAAWAAFAKDASGTGPWKMSKFTPRERLELVK
NEAYWDKARVPKADKMILLPMPEANARTAALLSGQVDWVEAPAPDALPEIKQRGFKLYANEQPHVWPWQFSRVEGSPWND
IRVRKAANLCIDREGLRDGLLAGLMVPATGTFEKGHPWRGNVTFEIKYDKAAAQKLMQEAGYGPNKKLEVKTQTSASGSG
QMQPLPMNEYLQQALAECYFDVKLDVIEWNTLFTNWRRGAKDASANGANATNVTYAAMDPFFALVRFLQSSMAPPVSNNW
GFINNPKFDELVKKARQTFDPAERDKALAELHAASVDDAAFLYVAHDVGPRAMSPKVKGVVQPKSWFIDFSPISVEP

Sequences:

>Translated_557_residues
MLLRLAPSVTKRAAIGAAMMTSAAILMLPATAGAESVLRIGMTAADIPRTLGQPDQGFEGNRFTGLTMYDALTMWDLSSA
DKPSVVIPGLATEWKVDESDKTKWTFKLRPGVTFHDGAPFNADAVVWNVEKVLKQDAPQFDPSQVGVTASRMPTLASARK
IDDLTVELVTKEPDSFLPINLTNLFMASPAKWQKLYESATGADDKAKSQAAWAAFAKDASGTGPWKMSKFTPRERLELVK
NEAYWDKARVPKADKMILLPMPEANARTAALLSGQVDWVEAPAPDALPEIKQRGFKLYANEQPHVWPWQFSRVEGSPWND
IRVRKAANLCIDREGLRDGLLAGLMVPATGTFEKGHPWRGNVTFEIKYDKAAAQKLMQEAGYGPNKKLEVKTQTSASGSG
QMQPLPMNEYLQQALAECYFDVKLDVIEWNTLFTNWRRGAKDASANGANATNVTYAAMDPFFALVRFLQSSMAPPVSNNW
GFINNPKFDELVKKARQTFDPAERDKALAELHAASVDDAAFLYVAHDVGPRAMSPKVKGVVQPKSWFIDFSPISVEP
>Mature_557_residues
MLLRLAPSVTKRAAIGAAMMTSAAILMLPATAGAESVLRIGMTAADIPRTLGQPDQGFEGNRFTGLTMYDALTMWDLSSA
DKPSVVIPGLATEWKVDESDKTKWTFKLRPGVTFHDGAPFNADAVVWNVEKVLKQDAPQFDPSQVGVTASRMPTLASARK
IDDLTVELVTKEPDSFLPINLTNLFMASPAKWQKLYESATGADDKAKSQAAWAAFAKDASGTGPWKMSKFTPRERLELVK
NEAYWDKARVPKADKMILLPMPEANARTAALLSGQVDWVEAPAPDALPEIKQRGFKLYANEQPHVWPWQFSRVEGSPWND
IRVRKAANLCIDREGLRDGLLAGLMVPATGTFEKGHPWRGNVTFEIKYDKAAAQKLMQEAGYGPNKKLEVKTQTSASGSG
QMQPLPMNEYLQQALAECYFDVKLDVIEWNTLFTNWRRGAKDASANGANATNVTYAAMDPFFALVRFLQSSMAPPVSNNW
GFINNPKFDELVKKARQTFDPAERDKALAELHAASVDDAAFLYVAHDVGPRAMSPKVKGVVQPKSWFIDFSPISVEP

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=530, Percent_Identity=24.7169811320755, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1787052, Length=468, Percent_Identity=25, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1787762, Length=470, Percent_Identity=23.8297872340426, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1787495, Length=552, Percent_Identity=22.6449275362319, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI87081878, Length=487, Percent_Identity=23.6139630390144, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1787551, Length=475, Percent_Identity=23.1578947368421, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 61359; Mature: 61359

Theoretical pI: Translated: 8.16; Mature: 8.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLRLAPSVTKRAAIGAAMMTSAAILMLPATAGAESVLRIGMTAADIPRTLGQPDQGFEG
CEEECCCCHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHCCCHHHHHHHHCCCCCCCCC
NRFTGLTMYDALTMWDLSSADKPSVVIPGLATEWKVDESDKTKWTFKLRPGVTFHDGAPF
CCEEEEEHHHHEEEEECCCCCCCCEEECCCCCCCCCCCCCCCEEEEEECCCEEECCCCCC
NADAVVWNVEKVLKQDAPQFDPSQVGVTASRMPTLASARKIDDLTVELVTKEPDSFLPIN
CCCEEEEEHHHHHHHCCCCCCCHHCCEEHHHCCHHHHHHCCCCEEEEEEECCCCCEECEE
LTNLFMASPAKWQKLYESATGADDKAKSQAAWAAFAKDASGTGPWKMSKFTPRERLELVK
HHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHH
NEAYWDKARVPKADKMILLPMPEANARTAALLSGQVDWVEAPAPDALPEIKQRGFKLYAN
CCHHHHHHCCCCCCCEEEEECCCCCCCEEEEECCCCCEECCCCCCCCHHHHHCCEEEEEC
EQPHVWPWQFSRVEGSPWNDIRVRKAANLCIDREGLRDGLLAGLMVPATGTFEKGHPWRG
CCCCCCCCEEECCCCCCCCCEEHHHHHHHHCCHHHHHHHHHHHEEECCCCCCCCCCCCCC
NVTFEIKYDKAAAQKLMQEAGYGPNKKLEVKTQTSASGSGQMQPLPMNEYLQQALAECYF
CEEEEEEECHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHEE
DVKLDVIEWNTLFTNWRRGAKDASANGANATNVTYAAMDPFFALVRFLQSSMAPPVSNNW
CEEEEEEECHHHHHHHHCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCC
GFINNPKFDELVKKARQTFDPAERDKALAELHAASVDDAAFLYVAHDVGPRAMSPKVKGV
CCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCEE
VQPKSWFIDFSPISVEP
ECCCCEEEEECCCCCCC
>Mature Secondary Structure
MLLRLAPSVTKRAAIGAAMMTSAAILMLPATAGAESVLRIGMTAADIPRTLGQPDQGFEG
CEEECCCCHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHCCCHHHHHHHHCCCCCCCCC
NRFTGLTMYDALTMWDLSSADKPSVVIPGLATEWKVDESDKTKWTFKLRPGVTFHDGAPF
CCEEEEEHHHHEEEEECCCCCCCCEEECCCCCCCCCCCCCCCEEEEEECCCEEECCCCCC
NADAVVWNVEKVLKQDAPQFDPSQVGVTASRMPTLASARKIDDLTVELVTKEPDSFLPIN
CCCEEEEEHHHHHHHCCCCCCCHHCCEEHHHCCHHHHHHCCCCEEEEEEECCCCCEECEE
LTNLFMASPAKWQKLYESATGADDKAKSQAAWAAFAKDASGTGPWKMSKFTPRERLELVK
HHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHH
NEAYWDKARVPKADKMILLPMPEANARTAALLSGQVDWVEAPAPDALPEIKQRGFKLYAN
CCHHHHHHCCCCCCCEEEEECCCCCCCEEEEECCCCCEECCCCCCCCHHHHHCCEEEEEC
EQPHVWPWQFSRVEGSPWNDIRVRKAANLCIDREGLRDGLLAGLMVPATGTFEKGHPWRG
CCCCCCCCEEECCCCCCCCCEEHHHHHHHHCCHHHHHHHHHHHEEECCCCCCCCCCCCCC
NVTFEIKYDKAAAQKLMQEAGYGPNKKLEVKTQTSASGSGQMQPLPMNEYLQQALAECYF
CEEEEEEECHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHEE
DVKLDVIEWNTLFTNWRRGAKDASANGANATNVTYAAMDPFFALVRFLQSSMAPPVSNNW
CEEEEEEECHHHHHHHHCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCC
GFINNPKFDELVKKARQTFDPAERDKALAELHAASVDDAAFLYVAHDVGPRAMSPKVKGV
CCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCEE
VQPKSWFIDFSPISVEP
ECCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA