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The map label for this gene is gsiB [H]

Identifier: 146338009

GI number: 146338009

Start: 961415

End: 962998

Strand: Reverse

Name: gsiB [H]

Synonym: BRADO0902

Alternate gene names: 146338009

Gene position: 962998-961415 (Counterclockwise)

Preceding gene: 146338020

Following gene: 146338006

Centisome position: 12.91

GC content: 63.7

Gene sequence:

>1584_bases
TTGTCCTTGCTCGCCGCCGCTCTTGCGCTCGGCCTTTCAGCCGTTGCGGCCCGCGCCGAAACTGTTCTGCGCTACGGCAT
CTCGATGGCCGACATCCCGCTCACGACAGGCCAGCCCGACCGCGGCGCCGGCGCCTATCAGTTTACCGGCTACACGATCT
ACGATCCGCTTGTCGCGTGGGACATGAGCGTCGCCGACAAGCCCGGCAAGCTGATCCCCGGCCTCGCCACCGAGTGGAAG
GTCGACGACGCCGACAAGAAGAAGTGGCGCTTCACCTTGCGCAAGGGCGTCAAGTTCCACGACGGCAGCGACTTCAATGC
CGACGCAGTGATCTGGAATCTGGACAAGGTGCTCAACGATAAGGCACCGCAGTTCGACAAGCGGCAGAGCGCACAGGTCA
AGACGCGGCTGCCCTCGGTCGCGAGCTACGCCAAGATCGACGACTCCACCGTGGAGATCACGACCAAGACGGTCGACTCG
TTCTTTCCTTATCAGATGCTGTGGTTCCTGATTTCGAGCCCGGCGCAGTATGAGAAGCTCGGCAAGGACTGGGACAAGTT
CGCCAGCCAGCCCTCGGGCACCGGCCCGTTCAAGCTGACCAGACTGGTGCCGCGCGAGCTCGCGGAACTGTCAAGGAATT
CCGAATATTGGAACAAGGACCGGATCCCGAAGACGGACAAGCTGGTGCTGGTGCCGATGCCGGAAGCGCTGACGCGTACC
AACGCGCTGCTCGCCGGCCAGGTCGATCTGATCGAGACCCCCGCGCCGGACGCCGTGCCGCAGCTCAAGGCCGCCGGCAT
GAAGCTCGTCGACAACGTCACGCCGCATGTCTGGAACTACCATCTCAGCGTTCTTCCAGGCTCGCCCTGGACCGACATCC
GCCTCCGCAAGGCCCTCAACCTCGCGATCAACCGCGACGAGGTCGTCGGCCTGATGAACGGTCTCGCCAAGCCGGCCAAG
GGCCAGGTCGACCCGTCGAGCCCGTGGTTCGGCAAGCCGACCTTCGAGCTGAAATATGATCTCGCTGCGGCCAAGAAGCT
GGTCGAGGAAGCCGGCTACTCGCCGGCCAAGCCGCTGAAGACCACCTTCATCATCGCGCAGGGCGGCACCGGGCAGATGC
TGTCGCTGCCGATGAACGAGTTCCTGCAGCAGAGCTTCAAGGAGATCGGCATCGACATCGACTTCAAGGTCGTCGAGCTG
GAGACGCTGTACACGCACTGGCGCAAGGGCGCGGCGGACGAGATGAACGCCGGCATCACCGCCAACAACATCGCCTATGT
CACGTCCGATCCGCTCTACGCCATCGTGCGCTTCTTCGCCTCCGACCAGATCGCGCCGACAGGCGTCAACTGGGGTGGCT
ACAAGAACCCGAAGGTCGATGCGCTGATCGCCGAGGCGAAGCAGACGTTCGATCCCGCCAAGCAGGACGAGCTGATCGCG
CAGGCGCATGCGGCTGTCGTCGATGATGCCGTGCTGGTCTGGGTCGTGCACGACACCAATCCGCACGCGCTGTCGCCGAA
GGTGAAGACATTCGTCCAGGCCCAGCACTGGTTCCAGGACCTGACGACGATCGGGGCGGAGTAG

Upstream 100 bases:

>100_bases
TGTGCTCAAACGATCCCGCGGGGCTTGGCACCCGGATTGCAGGTTTGGCTCATCGAATTTGATCCGGTTTCCACGGAGCT
TCACCCTGATGCGTATGAAA

Downstream 100 bases:

>100_bases
TGAACCGTCGTTCCGGCCTCGAGCCGGGACCCATATTCCGCGGCGGCGGTTGGCGGGCTGGCTAATGCCACGACCAGCGC
GGCCACCAATCAAAGCCGGT

Product: ABC transporter substrate-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 527; Mature: 526

Protein sequence:

>527_residues
MSLLAAALALGLSAVAARAETVLRYGISMADIPLTTGQPDRGAGAYQFTGYTIYDPLVAWDMSVADKPGKLIPGLATEWK
VDDADKKKWRFTLRKGVKFHDGSDFNADAVIWNLDKVLNDKAPQFDKRQSAQVKTRLPSVASYAKIDDSTVEITTKTVDS
FFPYQMLWFLISSPAQYEKLGKDWDKFASQPSGTGPFKLTRLVPRELAELSRNSEYWNKDRIPKTDKLVLVPMPEALTRT
NALLAGQVDLIETPAPDAVPQLKAAGMKLVDNVTPHVWNYHLSVLPGSPWTDIRLRKALNLAINRDEVVGLMNGLAKPAK
GQVDPSSPWFGKPTFELKYDLAAAKKLVEEAGYSPAKPLKTTFIIAQGGTGQMLSLPMNEFLQQSFKEIGIDIDFKVVEL
ETLYTHWRKGAADEMNAGITANNIAYVTSDPLYAIVRFFASDQIAPTGVNWGGYKNPKVDALIAEAKQTFDPAKQDELIA
QAHAAVVDDAVLVWVVHDTNPHALSPKVKTFVQAQHWFQDLTTIGAE

Sequences:

>Translated_527_residues
MSLLAAALALGLSAVAARAETVLRYGISMADIPLTTGQPDRGAGAYQFTGYTIYDPLVAWDMSVADKPGKLIPGLATEWK
VDDADKKKWRFTLRKGVKFHDGSDFNADAVIWNLDKVLNDKAPQFDKRQSAQVKTRLPSVASYAKIDDSTVEITTKTVDS
FFPYQMLWFLISSPAQYEKLGKDWDKFASQPSGTGPFKLTRLVPRELAELSRNSEYWNKDRIPKTDKLVLVPMPEALTRT
NALLAGQVDLIETPAPDAVPQLKAAGMKLVDNVTPHVWNYHLSVLPGSPWTDIRLRKALNLAINRDEVVGLMNGLAKPAK
GQVDPSSPWFGKPTFELKYDLAAAKKLVEEAGYSPAKPLKTTFIIAQGGTGQMLSLPMNEFLQQSFKEIGIDIDFKVVEL
ETLYTHWRKGAADEMNAGITANNIAYVTSDPLYAIVRFFASDQIAPTGVNWGGYKNPKVDALIAEAKQTFDPAKQDELIA
QAHAAVVDDAVLVWVVHDTNPHALSPKVKTFVQAQHWFQDLTTIGAE
>Mature_526_residues
SLLAAALALGLSAVAARAETVLRYGISMADIPLTTGQPDRGAGAYQFTGYTIYDPLVAWDMSVADKPGKLIPGLATEWKV
DDADKKKWRFTLRKGVKFHDGSDFNADAVIWNLDKVLNDKAPQFDKRQSAQVKTRLPSVASYAKIDDSTVEITTKTVDSF
FPYQMLWFLISSPAQYEKLGKDWDKFASQPSGTGPFKLTRLVPRELAELSRNSEYWNKDRIPKTDKLVLVPMPEALTRTN
ALLAGQVDLIETPAPDAVPQLKAAGMKLVDNVTPHVWNYHLSVLPGSPWTDIRLRKALNLAINRDEVVGLMNGLAKPAKG
QVDPSSPWFGKPTFELKYDLAAAKKLVEEAGYSPAKPLKTTFIIAQGGTGQMLSLPMNEFLQQSFKEIGIDIDFKVVELE
TLYTHWRKGAADEMNAGITANNIAYVTSDPLYAIVRFFASDQIAPTGVNWGGYKNPKVDALIAEAKQTFDPAKQDELIAQ
AHAAVVDDAVLVWVVHDTNPHALSPKVKTFVQAQHWFQDLTTIGAE

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=435, Percent_Identity=28.0459770114943, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1787495, Length=475, Percent_Identity=25.0526315789474, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI1789966, Length=554, Percent_Identity=25.0902527075812, Blast_Score=113, Evalue=4e-26,
Organism=Escherichia coli, GI1789397, Length=466, Percent_Identity=22.9613733905579, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1789887, Length=482, Percent_Identity=25.3112033195021, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787762, Length=473, Percent_Identity=25.369978858351, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI87081878, Length=480, Percent_Identity=22.5, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1787551, Length=316, Percent_Identity=27.8481012658228, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 58132; Mature: 58001

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLLAAALALGLSAVAARAETVLRYGISMADIPLTTGQPDRGAGAYQFTGYTIYDPLVAW
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCEEEECHHHHH
DMSVADKPGKLIPGLATEWKVDDADKKKWRFTLRKGVKFHDGSDFNADAVIWNLDKVLND
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEECCCCCCCCCEEEEEHHHHHCC
KAPQFDKRQSAQVKTRLPSVASYAKIDDSTVEITTKTVDSFFPYQMLWFLISSPAQYEKL
CCCCCCCHHHHHHHHHCCCHHHHHCCCCCEEEEEEHHHHHHCHHHHHHHHHCCCHHHHHH
GKDWDKFASQPSGTGPFKLTRLVPRELAELSRNSEYWNKDRIPKTDKLVLVPMPEALTRT
CCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCHHHHHC
NALLAGQVDLIETPAPDAVPQLKAAGMKLVDNVTPHVWNYHLSVLPGSPWTDIRLRKALN
CCEEECCEEEECCCCCCCHHHHHHHCHHHHHCCCCCEEEEEEEEECCCCCCHHHHHHHHH
LAINRDEVVGLMNGLAKPAKGQVDPSSPWFGKPTFELKYDLAAAKKLVEEAGYSPAKPLK
HHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHCCCCCCCCCE
TTFIIAQGGTGQMLSLPMNEFLQQSFKEIGIDIDFKVVELETLYTHWRKGAADEMNAGIT
EEEEEEECCCCCEEECCHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHCCCCHHHCCCEE
ANNIAYVTSDPLYAIVRFFASDQIAPTGVNWGGYKNPKVDALIAEAKQTFDPAKQDELIA
ECCEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHH
QAHAAVVDDAVLVWVVHDTNPHALSPKVKTFVQAQHWFQDLTTIGAE
HHHHHHHCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SLLAAALALGLSAVAARAETVLRYGISMADIPLTTGQPDRGAGAYQFTGYTIYDPLVAW
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCEEEECHHHHH
DMSVADKPGKLIPGLATEWKVDDADKKKWRFTLRKGVKFHDGSDFNADAVIWNLDKVLND
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEECCCCCCCCCEEEEEHHHHHCC
KAPQFDKRQSAQVKTRLPSVASYAKIDDSTVEITTKTVDSFFPYQMLWFLISSPAQYEKL
CCCCCCCHHHHHHHHHCCCHHHHHCCCCCEEEEEEHHHHHHCHHHHHHHHHCCCHHHHHH
GKDWDKFASQPSGTGPFKLTRLVPRELAELSRNSEYWNKDRIPKTDKLVLVPMPEALTRT
CCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCHHHHHC
NALLAGQVDLIETPAPDAVPQLKAAGMKLVDNVTPHVWNYHLSVLPGSPWTDIRLRKALN
CCEEECCEEEECCCCCCCHHHHHHHCHHHHHCCCCCEEEEEEEEECCCCCCHHHHHHHHH
LAINRDEVVGLMNGLAKPAKGQVDPSSPWFGKPTFELKYDLAAAKKLVEEAGYSPAKPLK
HHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHCCCCCCCCCE
TTFIIAQGGTGQMLSLPMNEFLQQSFKEIGIDIDFKVVELETLYTHWRKGAADEMNAGIT
EEEEEEECCCCCEEECCHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHCCCCHHHCCCEE
ANNIAYVTSDPLYAIVRFFASDQIAPTGVNWGGYKNPKVDALIAEAKQTFDPAKQDELIA
ECCEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHH
QAHAAVVDDAVLVWVVHDTNPHALSPKVKTFVQAQHWFQDLTTIGAE
HHHHHHHCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA