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The map label for this gene is pucL [H]

Identifier: 146337975

GI number: 146337975

Start: 918891

End: 920318

Strand: Direct

Name: pucL [H]

Synonym: BRADO0867

Alternate gene names: 146337975

Gene position: 918891-920318 (Clockwise)

Preceding gene: 146337974

Following gene: 146337978

Centisome position: 12.32

GC content: 65.13

Gene sequence:

>1428_bases
ATGAGCGCTGACGTTTACCCGCGCGACATGGTCGGTTACGGCCGCACGCCGCCGCATCCGCGCTGGCCAGGCGACGCCCG
CATCGCCGTGCAGTTCGTGATCAACTACGAGGAAGGTGGCGAGAACAACATCCTCCACGGCGACACGGCCTCCGAAGCGT
TCCTGTCCGAGATCGTCGGCGCGCAGCCCTGGTGGGGCCAGCGCCACATGAACATGGAATCGATCTACGAGTTCGGCTCG
CGGGCCGGCTTCTGGCGGCTGTGGCGGATGTTCACCGCACGCAAGCTGCCGGTGACGGTGTTCGCCATCGCCACCGCAAT
GGCTCGCAACCCGGATGCTGTGGCCGCCATGAAGGAGGCGGGTTGGGAGATCGCCTCTCACGGCCTGAAATGGATCGACT
ATCGCGACTTCTCCGAGGCCGACGAGCGTGCCCATATCGAGCAGGCAGTCCGTATCCACACCGAGCTGACCGGCGAACGG
CCGCTCGGCTTCTACCAGGGTCGCACCTCGGCGCACACGCTCGACATCGTCCTCAACGAGCAGGGCTTCGTCTACAGCGC
CGACTCCTATGCCGACGAGCTGCCGTACTGGATCCAGGGTCCGCACGGTCCGCAGCTGATCGTGCCCTATACGCTCGACG
CCAATGACATGCGCTTTGCGACACCGCAGGGCTTCAATGCGGGCGACCAGTTCTTTGCCTATCTGAAGGACAGTTTTGAT
ACGCTCTATGCCGAGGGTGCCGAAAGCCCGAAGATGATGTCGGTCGGGCTGCACTGTCGTCTCGTCGGCCGCCCGGGCCG
CGCCGCGGCGCTGGCGCGCTTCCTCGACTACGTCGTGGGTCATGAGAAGGTCTGGGTTGCGACACGTCTCGACATCGCCC
GGCATTGGATCCGGACGCATCCGCCGGCCGGTGTCTGGCGTCCGTCACAGATGTCGCGCGTGCTGTTCGTTGAGCGCTAT
GGCGACATCTTCGAGCATACGCCCGAGATCGCCGACCGTGCCTATTGTGCCGGCCTCAGCGCGTTCGACGATGCAGCGGA
CGCGTTGCATACGACCTTAATGAACGTCGTCCGCGCCATGAGCCGCGAAGAGAAGCTGAAGCTGATTCGCGCCCATCCCG
AGCTCGCCGGCCGCGAGGCGCTGGCTGGCCAGATGACCAGCGACTCCGTGGGCGAGCAGGGCCGGCTCGGCTTCACCTCG
CTCTCACGTTCCGAATTCGAGCGCGTCGCCGACATCAACCGCCGCTACCGCGACAAGTTCGACATGCCGCTGATCGTCGC
CCTGGCGCTGCACGCCGACCGGGCCTCCGTCATCGCCGAAATGGACCGCCGCATCAGCAACGACCTCGACACCGAGATCG
CCAACGCGATCGACCAGATCGGCCACATCACGCGGGCGCGGTTGGCGAAGAAGTTCGATCAGGAGTGA

Upstream 100 bases:

>100_bases
TCCCGCTCCGCTTTGCGGTCGCCGATGCCGGCGCGCATTATCACGTGCCGCTGCTCGCGACGCCCTGGAGCTATCAGACC
TATCGCGGGAGTTGAGTGAG

Downstream 100 bases:

>100_bases
GAGCGAACGGTCTGGTGATCTGGGTCGACGCTGGCTGCCATGAGAGGCGGTGCGCCCAACTCTCCGCGCAAGCCCAGCGA
GGTGAGCACCCGCAATGCTG

Product: putative polysaccharide deacetylase family protein

Products: NA

Alternate protein names: 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase; OHCU decarboxylase; Uricase; Urate oxidase [H]

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MSADVYPRDMVGYGRTPPHPRWPGDARIAVQFVINYEEGGENNILHGDTASEAFLSEIVGAQPWWGQRHMNMESIYEFGS
RAGFWRLWRMFTARKLPVTVFAIATAMARNPDAVAAMKEAGWEIASHGLKWIDYRDFSEADERAHIEQAVRIHTELTGER
PLGFYQGRTSAHTLDIVLNEQGFVYSADSYADELPYWIQGPHGPQLIVPYTLDANDMRFATPQGFNAGDQFFAYLKDSFD
TLYAEGAESPKMMSVGLHCRLVGRPGRAAALARFLDYVVGHEKVWVATRLDIARHWIRTHPPAGVWRPSQMSRVLFVERY
GDIFEHTPEIADRAYCAGLSAFDDAADALHTTLMNVVRAMSREEKLKLIRAHPELAGREALAGQMTSDSVGEQGRLGFTS
LSRSEFERVADINRRYRDKFDMPLIVALALHADRASVIAEMDRRISNDLDTEIANAIDQIGHITRARLAKKFDQE

Sequences:

>Translated_475_residues
MSADVYPRDMVGYGRTPPHPRWPGDARIAVQFVINYEEGGENNILHGDTASEAFLSEIVGAQPWWGQRHMNMESIYEFGS
RAGFWRLWRMFTARKLPVTVFAIATAMARNPDAVAAMKEAGWEIASHGLKWIDYRDFSEADERAHIEQAVRIHTELTGER
PLGFYQGRTSAHTLDIVLNEQGFVYSADSYADELPYWIQGPHGPQLIVPYTLDANDMRFATPQGFNAGDQFFAYLKDSFD
TLYAEGAESPKMMSVGLHCRLVGRPGRAAALARFLDYVVGHEKVWVATRLDIARHWIRTHPPAGVWRPSQMSRVLFVERY
GDIFEHTPEIADRAYCAGLSAFDDAADALHTTLMNVVRAMSREEKLKLIRAHPELAGREALAGQMTSDSVGEQGRLGFTS
LSRSEFERVADINRRYRDKFDMPLIVALALHADRASVIAEMDRRISNDLDTEIANAIDQIGHITRARLAKKFDQE
>Mature_474_residues
SADVYPRDMVGYGRTPPHPRWPGDARIAVQFVINYEEGGENNILHGDTASEAFLSEIVGAQPWWGQRHMNMESIYEFGSR
AGFWRLWRMFTARKLPVTVFAIATAMARNPDAVAAMKEAGWEIASHGLKWIDYRDFSEADERAHIEQAVRIHTELTGERP
LGFYQGRTSAHTLDIVLNEQGFVYSADSYADELPYWIQGPHGPQLIVPYTLDANDMRFATPQGFNAGDQFFAYLKDSFDT
LYAEGAESPKMMSVGLHCRLVGRPGRAAALARFLDYVVGHEKVWVATRLDIARHWIRTHPPAGVWRPSQMSRVLFVERYG
DIFEHTPEIADRAYCAGLSAFDDAADALHTTLMNVVRAMSREEKLKLIRAHPELAGREALAGQMTSDSVGEQGRLGFTSL
SRSEFERVADINRRYRDKFDMPLIVALALHADRASVIAEMDRRISNDLDTEIANAIDQIGHITRARLAKKFDQE

Specific function: Catalyzes two steps in the degradation of uric acid, i.e. the oxidation of uric acid to 5-hydroxyisourate (HIU) and the stereoselective decarboxylation of 2-oxo-4-hydroxy-4-carboxy-5- ureidoimidazoline (OHCU) to (S)-allantoin [H]

COG id: COG0726

COG function: function code G; Predicted xylanase/chitin deacetylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the uricase family [H]

Homologues:

Organism=Homo sapiens, GI157743280, Length=154, Percent_Identity=31.8181818181818, Blast_Score=71, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018020
- InterPro:   IPR017580
- InterPro:   IPR002042
- InterPro:   IPR019842 [H]

Pfam domain/function: PF09349 OHCU_decarbox; PF01014 Uricase [H]

EC number: =1.7.3.3 [H]

Molecular weight: Translated: 53621; Mature: 53490

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSADVYPRDMVGYGRTPPHPRWPGDARIAVQFVINYEEGGENNILHGDTASEAFLSEIVG
CCCCCCCHHHCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHC
AQPWWGQRHMNMESIYEFGSRAGFWRLWRMFTARKLPVTVFAIATAMARNPDAVAAMKEA
CCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHH
GWEIASHGLKWIDYRDFSEADERAHIEQAVRIHTELTGERPLGFYQGRTSAHTLDIVLNE
CHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECC
QGFVYSADSYADELPYWIQGPHGPQLIVPYTLDANDMRFATPQGFNAGDQFFAYLKDSFD
CCCEEECCCHHHHCCCEEECCCCCEEEEEEEECCCCCEECCCCCCCCHHHHHHHHHHHHH
TLYAEGAESPKMMSVGLHCRLVGRPGRAAALARFLDYVVGHEKVWVATRLDIARHWIRTH
HHHHCCCCCCCEEEECEEEEEECCCCHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHCC
PPAGVWRPSQMSRVLFVERYGDIFEHTPEIADRAYCAGLSAFDDAADALHTTLMNVVRAM
CCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
SREEKLKLIRAHPELAGREALAGQMTSDSVGEQGRLGFTSLSRSEFERVADINRRYRDKF
HHHHHHHHHHCCCHHHCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
DMPLIVALALHADRASVIAEMDRRISNDLDTEIANAIDQIGHITRARLAKKFDQE
CCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SADVYPRDMVGYGRTPPHPRWPGDARIAVQFVINYEEGGENNILHGDTASEAFLSEIVG
CCCCCCHHHCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHC
AQPWWGQRHMNMESIYEFGSRAGFWRLWRMFTARKLPVTVFAIATAMARNPDAVAAMKEA
CCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHH
GWEIASHGLKWIDYRDFSEADERAHIEQAVRIHTELTGERPLGFYQGRTSAHTLDIVLNE
CHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECC
QGFVYSADSYADELPYWIQGPHGPQLIVPYTLDANDMRFATPQGFNAGDQFFAYLKDSFD
CCCEEECCCHHHHCCCEEECCCCCEEEEEEEECCCCCEECCCCCCCCHHHHHHHHHHHHH
TLYAEGAESPKMMSVGLHCRLVGRPGRAAALARFLDYVVGHEKVWVATRLDIARHWIRTH
HHHHCCCCCCCEEEECEEEEEECCCCHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHCC
PPAGVWRPSQMSRVLFVERYGDIFEHTPEIADRAYCAGLSAFDDAADALHTTLMNVVRAM
CCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
SREEKLKLIRAHPELAGREALAGQMTSDSVGEQGRLGFTSLSRSEFERVADINRRYRDKF
HHHHHHHHHHCCCHHHCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
DMPLIVALALHADRASVIAEMDRRISNDLDTEIANAIDQIGHITRARLAKKFDQE
CCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11344136 [H]