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The map label for this gene is ada [H]

Identifier: 146337323

GI number: 146337323

Start: 168384

End: 169283

Strand: Direct

Name: ada [H]

Synonym: BRADO0154

Alternate gene names: 146337323

Gene position: 168384-169283 (Clockwise)

Preceding gene: 146337322

Following gene: 146337334

Centisome position: 2.26

GC content: 70.33

Gene sequence:

>900_bases
ATGATGACCCTCGCCATTCACGATTCCGCCCTGGCCAAGCCGGGCCCGCAACAATCCGCCGCGCTGCGCGACTACGATGC
AGTGCGCCGCGCGATCGCGTTCATCTCCGAGCACTGGCGCCGGCAGCCGACCATCGAGGACATGGCCGACGCCGCCAGCC
TGACGCCGGACGAGCTGCACCATCTGTTCCGGCGCTGGGCCGGGCTGACGCCGAAGGCGTTCATGCAGGCGCTGACGCTC
GACCACGCCAAGGGCCTGCTGAAGGGCTCGGCCAGCGTGCTCGACGCCGCGCTCGACTCCGGCCTGTCGGGTCCCGGCCG
CCTGCACGATCTGTTCGTCACCCACGAGGCGATGTCTCCCGGCGAATGGAAGACCGGCGGCGCCGGCACCACCTTGCGCT
ACGGCTTCCATCCCTGCCCGTTCGGCATGGCGCTGGTCATGGCCACCCCGCGCGGCCTCGCCGGCCTGGCCTTCGCCGAC
CCCGGCGAGGAGGCTGGCGCGCTCGCCGACATGAAGAGCCGCTGGCCCCGCGCCACTTACGTCGAGGACGTCCCCGGCAC
CGCGGCGCTCGCGCAGCGCATCTTCGACAAGAACCACTGGCGCCCCGAGCAACCCTTACGCGTCGTCCTGATCGGCACCG
ATTTCGAGGTCCGCGTCTGGGAAGCCCTGCTCAAGATCCCCATGGGCCGCGCCGTCTGCTACTCGGACATCGCGACAAGG
CTGGAGTCACCGAAGGCCTCGCGCGCGGTCGGCGCGGCGGTGGGCAAGAACCCGATCTCGTTCGTGGTCCCGTGCCATCG
CGCGCTGGGGAAGACGGGCGCGTTGACAGGGTATCATTGGGGCCTCACGCGCAAGCAGGCGATGATCGGGTGGGAGGCTG
GGCAGGTGGGGATGGGGTAG

Upstream 100 bases:

>100_bases
TGAGGCCCGGAAAAACGCCGCGCGACCGCCCGCGCCACCCCTAGCCCCCCAATCAGAAATCGGGTGGAGTGCCCTCCCCG
GCCGCCCTACATCCGGACTC

Downstream 100 bases:

>100_bases
GCGTATCTACAATGCGCTATCTGGGTGACGTAGGCTGCGTTGAACGAGCCTTCCGAACTCGCCTGGCGGCGTGAACCCGA
TCGTACAGGCATCGCTCTAT

Product: putative methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: Regulatory protein of adaptative response; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MMTLAIHDSALAKPGPQQSAALRDYDAVRRAIAFISEHWRRQPTIEDMADAASLTPDELHHLFRRWAGLTPKAFMQALTL
DHAKGLLKGSASVLDAALDSGLSGPGRLHDLFVTHEAMSPGEWKTGGAGTTLRYGFHPCPFGMALVMATPRGLAGLAFAD
PGEEAGALADMKSRWPRATYVEDVPGTAALAQRIFDKNHWRPEQPLRVVLIGTDFEVRVWEALLKIPMGRAVCYSDIATR
LESPKASRAVGAAVGKNPISFVVPCHRALGKTGALTGYHWGLTRKQAMIGWEAGQVGMG

Sequences:

>Translated_299_residues
MMTLAIHDSALAKPGPQQSAALRDYDAVRRAIAFISEHWRRQPTIEDMADAASLTPDELHHLFRRWAGLTPKAFMQALTL
DHAKGLLKGSASVLDAALDSGLSGPGRLHDLFVTHEAMSPGEWKTGGAGTTLRYGFHPCPFGMALVMATPRGLAGLAFAD
PGEEAGALADMKSRWPRATYVEDVPGTAALAQRIFDKNHWRPEQPLRVVLIGTDFEVRVWEALLKIPMGRAVCYSDIATR
LESPKASRAVGAAVGKNPISFVVPCHRALGKTGALTGYHWGLTRKQAMIGWEAGQVGMG
>Mature_299_residues
MMTLAIHDSALAKPGPQQSAALRDYDAVRRAIAFISEHWRRQPTIEDMADAASLTPDELHHLFRRWAGLTPKAFMQALTL
DHAKGLLKGSASVLDAALDSGLSGPGRLHDLFVTHEAMSPGEWKTGGAGTTLRYGFHPCPFGMALVMATPRGLAGLAFAD
PGEEAGALADMKSRWPRATYVEDVPGTAALAQRIFDKNHWRPEQPLRVVLIGTDFEVRVWEALLKIPMGRAVCYSDIATR
LESPKASRAVGAAVGKNPISFVVPCHRALGKTGALTGYHWGLTRKQAMIGWEAGQVGMG

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB [H]

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788542, Length=254, Percent_Identity=29.5275590551181, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI1787596, Length=78, Percent_Identity=50, Blast_Score=85, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI115533070, Length=84, Percent_Identity=41.6666666666667, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI115533068, Length=84, Percent_Identity=41.6666666666667, Blast_Score=84, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6320001, Length=83, Percent_Identity=44.578313253012, Blast_Score=86, Evalue=5e-18,
Organism=Drosophila melanogaster, GI17137554, Length=175, Percent_Identity=29.7142857142857, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR016221
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF01035 DNA_binding_1; PF00165 HTH_AraC; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 32312; Mature: 32312

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: PS01124 HTH_ARAC_FAMILY_2 ; PS00678 WD_REPEATS_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTLAIHDSALAKPGPQQSAALRDYDAVRRAIAFISEHWRRQPTIEDMADAASLTPDELH
CEEEEECCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHH
HLFRRWAGLTPKAFMQALTLDHAKGLLKGSASVLDAALDSGLSGPGRLHDLFVTHEAMSP
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEEEECCCCCC
GEWKTGGAGTTLRYGFHPCPFGMALVMATPRGLAGLAFADPGEEAGALADMKSRWPRATY
CCCCCCCCCCEEECCCCCCCHHHEEEHCCCCCCCCCEECCCCCHHHHHHHHHHCCCCCCC
VEDVPGTAALAQRIFDKNHWRPEQPLRVVLIGTDFEVRVWEALLKIPMGRAVCYSDIATR
CCCCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHH
LESPKASRAVGAAVGKNPISFVVPCHRALGKTGALTGYHWGLTRKQAMIGWEAGQVGMG
HCCCCHHHHHHHHHCCCCCEEEEECHHHCCCCCCCCCEECCCCHHHHHEECCCCCCCCC
>Mature Secondary Structure
MMTLAIHDSALAKPGPQQSAALRDYDAVRRAIAFISEHWRRQPTIEDMADAASLTPDELH
CEEEEECCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHH
HLFRRWAGLTPKAFMQALTLDHAKGLLKGSASVLDAALDSGLSGPGRLHDLFVTHEAMSP
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEEEECCCCCC
GEWKTGGAGTTLRYGFHPCPFGMALVMATPRGLAGLAFADPGEEAGALADMKSRWPRATY
CCCCCCCCCCEEECCCCCCCHHHEEEHCCCCCCCCCEECCCCCHHHHHHHHHHCCCCCCC
VEDVPGTAALAQRIFDKNHWRPEQPLRVVLIGTDFEVRVWEALLKIPMGRAVCYSDIATR
CCCCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHH
LESPKASRAVGAAVGKNPISFVVPCHRALGKTGALTGYHWGLTRKQAMIGWEAGQVGMG
HCCCCHHHHHHHHHCCCCCEEEEECHHHCCCCCCCCCEECCCCHHHHHEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987251; 3887409; 9097040; 9278503; 2982792; 3536913; 3009022; 3529081; 1581309; 8202360; 8500619; 8156986 [H]