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The map label for this gene is gyaR [H]

Identifier: 146337240

GI number: 146337240

Start: 75734

End: 76735

Strand: Direct

Name: gyaR [H]

Synonym: BRADO0066

Alternate gene names: 146337240

Gene position: 75734-76735 (Clockwise)

Preceding gene: 146337238

Following gene: 146337242

Centisome position: 1.02

GC content: 66.17

Gene sequence:

>1002_bases
ATGTCAGGTAGGAAAAAGCCCCTCGTCGTCGTGACACGCAAGCTGCCGGACTCGGTCGAGACGCGCATGCGCGAGCTGTT
CGACGCGCGGCTCAATCTCGATGACGAGCCGATGAGCCAGGAGCAGCTCGCCGACGCCGTGCGCACCGCCGAGGTGCTGG
TTCCGACCGTCACCGATCACATCAGCGCGGATATTCTCAATCAACCCGACTGCAAGCTGAGGATGATCGCCAACTTCGGC
AACGGCGTCGACAACATCGACGTCGCCGCGGCCGTGGCGCGCGGCATCACCGTGACCAACACGCCGAAGGTGCTCACCGA
GGACACCGCCGACATGACCATGGCGCTGATCCTCGCCGTGCCGCGGCGGCTGATCGAGGGCGCCTCGGTGCTCACCGAGG
GCAAGAACTGGGCGGGCTGGTCGCCGACCTGGATGCTCGGCCATCGCATCGGCGGCAAGCGTCTCGGCATCATCGGCATG
GGCCGCATCGGCCAGGCGGTGGCGCGCCGCGCCCGCGCCTTCGGCCTGCAGATCCACTATCACAACCGCCGCCCGGTCGC
GCCGGTCATCGCCGACGAGCTTGGCGCTACCTACTGGGAAAGTCTCGACCAGATGCTGGCGCGGATGGACATCATCTCTG
TGAACTGTCCGCACACGCCTGCGACGTTCCACCTGCTGTCGGCGCGCCGGCTCAAGCTGATCCGCAAGGACGCCTATATC
GTCAACACCGCGCGCGGCGAGGTGATCGACGAGGAGACGATGACCCGGCTGATCGAAGCCGGCGACATCGCTGGCGCCGG
CCTCGACGTGTTCGAGCATGAGCCCGCCGTCAATCCGCGCCTCGTGCGGCTCGCCAAGGCCGGCAAGGTGACCTTGCTGC
CGCATATGGGCTCGGCGACCATCGAAGGCCGCGTCGAGATGGGCGAGAAGGTCATCATCAACATCAAGACCTTCCTCGAC
AACCACCGTCCGCCGGATCGCGTTCTGCCGAGCATGCTATAG

Upstream 100 bases:

>100_bases
ATCCGACACGACCAGTTCAGGGAACGCCTCTTCTCAGACGACGACGGTAGTTTCGGCGAACCCGGTTAATGAGGTCTGAA
GGATCTCATGAGAGCAGCAC

Downstream 100 bases:

>100_bases
GCCGACCTGCCGGCTACGCGATCGGTGGCGTCCCGGCCTGAGCCGTGACCCATACCGCGTGATCTCCGCAAGGAACGCGG
CTGGACGTGATCGTCCCACT

Product: putative D-isomer specific 2-hydroxyacid dehydrogenases family protein glyoxylate reductase (glycolate reductase)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MSGRKKPLVVVTRKLPDSVETRMRELFDARLNLDDEPMSQEQLADAVRTAEVLVPTVTDHISADILNQPDCKLRMIANFG
NGVDNIDVAAAVARGITVTNTPKVLTEDTADMTMALILAVPRRLIEGASVLTEGKNWAGWSPTWMLGHRIGGKRLGIIGM
GRIGQAVARRARAFGLQIHYHNRRPVAPVIADELGATYWESLDQMLARMDIISVNCPHTPATFHLLSARRLKLIRKDAYI
VNTARGEVIDEETMTRLIEAGDIAGAGLDVFEHEPAVNPRLVRLAKAGKVTLLPHMGSATIEGRVEMGEKVIINIKTFLD
NHRPPDRVLPSML

Sequences:

>Translated_333_residues
MSGRKKPLVVVTRKLPDSVETRMRELFDARLNLDDEPMSQEQLADAVRTAEVLVPTVTDHISADILNQPDCKLRMIANFG
NGVDNIDVAAAVARGITVTNTPKVLTEDTADMTMALILAVPRRLIEGASVLTEGKNWAGWSPTWMLGHRIGGKRLGIIGM
GRIGQAVARRARAFGLQIHYHNRRPVAPVIADELGATYWESLDQMLARMDIISVNCPHTPATFHLLSARRLKLIRKDAYI
VNTARGEVIDEETMTRLIEAGDIAGAGLDVFEHEPAVNPRLVRLAKAGKVTLLPHMGSATIEGRVEMGEKVIINIKTFLD
NHRPPDRVLPSML
>Mature_332_residues
SGRKKPLVVVTRKLPDSVETRMRELFDARLNLDDEPMSQEQLADAVRTAEVLVPTVTDHISADILNQPDCKLRMIANFGN
GVDNIDVAAAVARGITVTNTPKVLTEDTADMTMALILAVPRRLIEGASVLTEGKNWAGWSPTWMLGHRIGGKRLGIIGMG
RIGQAVARRARAFGLQIHYHNRRPVAPVIADELGATYWESLDQMLARMDIISVNCPHTPATFHLLSARRLKLIRKDAYIV
NTARGEVIDEETMTRLIEAGDIAGAGLDVFEHEPAVNPRLVRLAKAGKVTLLPHMGSATIEGRVEMGEKVIINIKTFLDN
HRPPDRVLPSML

Specific function: Unknown

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GyaR subfamily [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=302, Percent_Identity=34.1059602649007, Blast_Score=166, Evalue=2e-41,
Organism=Homo sapiens, GI23308577, Length=311, Percent_Identity=31.1897106109325, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI61743967, Length=263, Percent_Identity=33.8403041825095, Blast_Score=132, Evalue=3e-31,
Organism=Homo sapiens, GI4557497, Length=263, Percent_Identity=33.8403041825095, Blast_Score=132, Evalue=3e-31,
Organism=Homo sapiens, GI145580578, Length=254, Percent_Identity=34.251968503937, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI4557499, Length=254, Percent_Identity=34.251968503937, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI145580575, Length=254, Percent_Identity=34.251968503937, Blast_Score=128, Evalue=8e-30,
Organism=Escherichia coli, GI87082289, Length=312, Percent_Identity=36.5384615384615, Blast_Score=171, Evalue=7e-44,
Organism=Escherichia coli, GI1789279, Length=289, Percent_Identity=28.3737024221453, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1787645, Length=336, Percent_Identity=25.297619047619, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI87081824, Length=275, Percent_Identity=24.7272727272727, Blast_Score=61, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17532191, Length=258, Percent_Identity=32.5581395348837, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI25147481, Length=204, Percent_Identity=34.8039215686275, Blast_Score=114, Evalue=9e-26,
Organism=Saccharomyces cerevisiae, GI6324055, Length=247, Percent_Identity=38.4615384615385, Blast_Score=155, Evalue=6e-39,
Organism=Saccharomyces cerevisiae, GI6320925, Length=294, Percent_Identity=28.2312925170068, Blast_Score=125, Evalue=8e-30,
Organism=Saccharomyces cerevisiae, GI6322116, Length=259, Percent_Identity=29.7297297297297, Blast_Score=122, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6324964, Length=262, Percent_Identity=29.0076335877863, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6321253, Length=210, Percent_Identity=28.0952380952381, Blast_Score=77, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6325144, Length=165, Percent_Identity=32.1212121212121, Blast_Score=77, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6324980, Length=153, Percent_Identity=29.4117647058824, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24646446, Length=287, Percent_Identity=35.191637630662, Blast_Score=145, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24646448, Length=287, Percent_Identity=35.191637630662, Blast_Score=145, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24646452, Length=287, Percent_Identity=35.191637630662, Blast_Score=145, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24646450, Length=287, Percent_Identity=35.191637630662, Blast_Score=145, Evalue=3e-35,
Organism=Drosophila melanogaster, GI62472511, Length=287, Percent_Identity=35.191637630662, Blast_Score=144, Evalue=7e-35,
Organism=Drosophila melanogaster, GI28571528, Length=319, Percent_Identity=34.7962382445141, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI19921140, Length=271, Percent_Identity=30.6273062730627, Blast_Score=134, Evalue=9e-32,
Organism=Drosophila melanogaster, GI45552429, Length=272, Percent_Identity=30.5147058823529, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24585514, Length=272, Percent_Identity=30.5147058823529, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI28574282, Length=272, Percent_Identity=30.5147058823529, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI28574284, Length=272, Percent_Identity=30.5147058823529, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI45551003, Length=272, Percent_Identity=30.5147058823529, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI28574286, Length=302, Percent_Identity=29.1390728476821, Blast_Score=133, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24585516, Length=275, Percent_Identity=28, Blast_Score=115, Evalue=5e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.26 [H]

Molecular weight: Translated: 36673; Mature: 36542

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGRKKPLVVVTRKLPDSVETRMRELFDARLNLDDEPMSQEQLADAVRTAEVLVPTVTDH
CCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ISADILNQPDCKLRMIANFGNGVDNIDVAAAVARGITVTNTPKVLTEDTADMTMALILAV
HHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCEECCCCCEEECCHHHHHHHHHHHH
PRRLIEGASVLTEGKNWAGWSPTWMLGHRIGGKRLGIIGMGRIGQAVARRARAFGLQIHY
HHHHHCCHHHHCCCCCCCCCCCHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHEEEEEE
HNRRPVAPVIADELGATYWESLDQMLARMDIISVNCPHTPATFHLLSARRLKLIRKDAYI
CCCCCCCHHHHHHHCHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHCCEE
VNTARGEVIDEETMTRLIEAGDIAGAGLDVFEHEPAVNPRLVRLAKAGKVTLLPHMGSAT
EECCCCCEECHHHHHHHHHHCCCCCCCCHHHCCCCCCCHHHEEEECCCCEEEEECCCCCE
IEGRVEMGEKVIINIKTFLDNHRPPDRVLPSML
ECCCEECCCEEEEEEEHHHCCCCCHHHHCCCCC
>Mature Secondary Structure 
SGRKKPLVVVTRKLPDSVETRMRELFDARLNLDDEPMSQEQLADAVRTAEVLVPTVTDH
CCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ISADILNQPDCKLRMIANFGNGVDNIDVAAAVARGITVTNTPKVLTEDTADMTMALILAV
HHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCEECCCCCEEECCHHHHHHHHHHHH
PRRLIEGASVLTEGKNWAGWSPTWMLGHRIGGKRLGIIGMGRIGQAVARRARAFGLQIHY
HHHHHCCHHHHCCCCCCCCCCCHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHEEEEEE
HNRRPVAPVIADELGATYWESLDQMLARMDIISVNCPHTPATFHLLSARRLKLIRKDAYI
CCCCCCCHHHHHHHCHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHCCEE
VNTARGEVIDEETMTRLIEAGDIAGAGLDVFEHEPAVNPRLVRLAKAGKVTLLPHMGSAT
EECCCCCEECHHHHHHHHHHCCCCCCCCHHHCCCCCCCHHHEEEECCCCEEEEECCCCCE
IEGRVEMGEKVIINIKTFLDNHRPPDRVLPSML
ECCCEECCCEEEEEEEHHHCCCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA