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The map label for this gene is gyrB [H]

Identifier: 146337179

GI number: 146337179

Start: 5434

End: 7872

Strand: Direct

Name: gyrB [H]

Synonym: BRADO0004

Alternate gene names: 146337179

Gene position: 5434-7872 (Clockwise)

Preceding gene: 146337178

Following gene: 146337180

Centisome position: 0.07

GC content: 64.94

Gene sequence:

>2439_bases
ATGACCGAACCCGCCCGGCAAACCATCGCCGACAACGAGCCTGCCACCGCGAATGAATACGGCGCAGAATCGATCCGGGT
GCTGAAGGGCCTGGATGCCGTGCGCAAGCGCCCGGGCATGTATATCGGCGACACCGATGACGGCTCCGGCCTGCATCACA
TGGTCTACGAGGTCGTCGACAACGCGATCGACGAGGCGCTGGCCGGCTACGCGACCCGGGTGGAGGTCGTGCTCAACGCC
GACAATTCCGTCACCGTGCGCGACGACGGCCGCGGCATTCCTGTCGGCATTCACAAGGATGAAGGCGTCTCCGCGGCCGA
GGTCATCATGACCCAGCTGCACGCCGGCGGAAAGTTCGACCAGAACTCCTACAAGGTTTCCGGCGGCCTGCACGGCGTCG
GCGTCTCCGTCGTGAACGCGCTGTCGAGCAAGCTCGAGCTGCGTATCTGGCGCGAGGACAAGGAGCATCTGATCGAGTTC
GCCCATGGCGACGCGGTCGCGCCGCTGCGGGTGGTCGGCGAGTCCAAGGGCAAGCGCGGCACCGAGGTGACGTTCCTCGC
CTCGACCGAGACCTTCAAGATGGTCGAGTACGACTACGCGACCCTCGAGCATCGCCTGCGCGAGCTCGCCTTCCTCAACT
CCGGCGTCCACATCATCCTCTCCGACATGCGCCACGCGGTCGAGAAGCGCGAGGAGATGTTCTATTCCGGCGGCGTCGAG
GAGTTCGTCAAATATCTCGACCGCAACAAGAAGGCGATCGTTCCCAATCCGATCATGGTGCGCTCGGAAGCCAACGGCAT
CACCGTCGAGGCCGCGCTGTGGTGGAACGACAGCTACCATGAGAACGTGCTGTGCTTCACCAACAACATTCCGCAACGTG
ACGGCGGCACCCATCTGGCCGGCTTCCGCGGCGCGTTGACGCGTCAGGTCAACGGCTATGCCGAGGCCAACGCGAAGAAG
GAAAAGATCGCGCTGACCGGCGACGACTGCCGCGAAGGCCTCACCGCGGTGCTCTCGGTGAAGGTGCCGGATCCGAAGTT
TTCGTCGCAGACCAAGGACAAGCTGGTGTCCTCGGAAGTGCGCCCCGTCGTCGAGAACGTCATCAACGAGGCACTGTCGG
CATGGTTCGAGGAGCATCCCTCCGAAGCCAAGACCGTCGTCGGCAAGGTGATCCAGGCCGCCGCAGCCCGCGAAGCCGCG
CGAAAAGCGCGCGAGCTGACGCGCAAGAATCCGCTCAACAATATCGCTTCGCTCCCCGGCAAGCTCGCCGACTGCCAGGA
GAAGGATCCGGCCAAATCCGAGCTGTTCATCGTCGAGGGTGACTCGGCCGGCGGCAGCGCCAAGCAGGGCCGCAACCGCG
AGTTCCAGGCGGTGCTGCCGCTGCGCGGCAAGATCCTCAATGTCGAGCGCGTGCGTCCCGACAAGATGCTGTCGAGCGAG
CAGATCGGCACCTTGATCACCGCGCTCGGCACCGGGATCAGCGACGATTTCTCAATCGACAAGCTGCGCTATCACAAGAT
CATCGTGATGACCGACGCCGACGTCGACGGCGCCCACATCCGCACCCTGCTGCTGACGTTCTTCTACCGGCAGATGCGCC
AGATCATCGACCGCGGCCATCTCTACATCGCCCAGCCGCCGCTCTACAAAGTCACACGCGGCAAATCCGAGCAGTATTTG
AAGGACGAGCGCGCGCTCGAGGATTATCTGATCGGCACCGGTCTCGACGATTGCGTGTTCCGGACCGCCGACGGCGAGGA
GCGCAAGGGCCGCGACCTGCTGGCGCTGGTCGAGGAAGCCCGGCTCGTGCGCAACATCCTGCGCAATCTGCACAGCCGCT
ACGACCGAAAGGTCGTCGAGCAGGCGGCGATCGCCGGCGTGCTGCGCGGAGACATCACCCGCGACATCGCGACCGCGGAC
GCCGCCGCCGAATACATCGCCAAGCGGCTCGACGTGCTCGCTGAAGAGGTCGAGCGCGGCTGGATCGGCCATTTCGTCGA
GGGCCAGGGTTTTACGTTCGAGCGCACCGTGCGCGGCGTCAAGGACGTCGCTGTCATCGACGATGCGCTGCTGGCGTCTG
CCGACGCCCGCAAGCTCGACGACTACGCGGCCCGTCTGCAGGACGCCTACGCCAAGCCCGGTGCCTTGCGCCGCGGCGAC
ACCCAGACCGTGATCCACGGCCCGGTCGACCTGTTCGAGGCCGTCACCGATTCCGGCCGCAAGGGCATCGCGATGCAGCG
CTACAAAGGGCTGGGCGAGATGAACCCGCAGCAGCTCTGGGAAACCACGCTCGACACCGAGGCCCGCACCTTGCTCCAGG
TGAAGGTCAAGGAGGTCGACGAGGCCGACGACATCTTCACCAAGCTGATGGGCGACGTCGTCGAGCCGCGCCGCGAGTTC
ATCCAGGACAACTCGCTGTCGGCGAATGTGGACGTGTAG

Upstream 100 bases:

>100_bases
CGCCTTTTTACGCTAGGCGCGCCTTCCGTTTCATGGCAGAAATAGCGTCCTCAGCACCCCAGCGCGACATCGCCCTACGG
GACCCCTTCAAAGGCCTCAC

Downstream 100 bases:

>100_bases
CGTAACTGAATTCCGTTCTTTGCTTCATCGGAAGCGAAGGACGGGACACTGGTTCTCGGATACTCTGGTTTTCGGATACC
CTGGTTCGTATACAGTGTGC

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 812; Mature: 811

Protein sequence:

>812_residues
MTEPARQTIADNEPATANEYGAESIRVLKGLDAVRKRPGMYIGDTDDGSGLHHMVYEVVDNAIDEALAGYATRVEVVLNA
DNSVTVRDDGRGIPVGIHKDEGVSAAEVIMTQLHAGGKFDQNSYKVSGGLHGVGVSVVNALSSKLELRIWREDKEHLIEF
AHGDAVAPLRVVGESKGKRGTEVTFLASTETFKMVEYDYATLEHRLRELAFLNSGVHIILSDMRHAVEKREEMFYSGGVE
EFVKYLDRNKKAIVPNPIMVRSEANGITVEAALWWNDSYHENVLCFTNNIPQRDGGTHLAGFRGALTRQVNGYAEANAKK
EKIALTGDDCREGLTAVLSVKVPDPKFSSQTKDKLVSSEVRPVVENVINEALSAWFEEHPSEAKTVVGKVIQAAAAREAA
RKARELTRKNPLNNIASLPGKLADCQEKDPAKSELFIVEGDSAGGSAKQGRNREFQAVLPLRGKILNVERVRPDKMLSSE
QIGTLITALGTGISDDFSIDKLRYHKIIVMTDADVDGAHIRTLLLTFFYRQMRQIIDRGHLYIAQPPLYKVTRGKSEQYL
KDERALEDYLIGTGLDDCVFRTADGEERKGRDLLALVEEARLVRNILRNLHSRYDRKVVEQAAIAGVLRGDITRDIATAD
AAAEYIAKRLDVLAEEVERGWIGHFVEGQGFTFERTVRGVKDVAVIDDALLASADARKLDDYAARLQDAYAKPGALRRGD
TQTVIHGPVDLFEAVTDSGRKGIAMQRYKGLGEMNPQQLWETTLDTEARTLLQVKVKEVDEADDIFTKLMGDVVEPRREF
IQDNSLSANVDV

Sequences:

>Translated_812_residues
MTEPARQTIADNEPATANEYGAESIRVLKGLDAVRKRPGMYIGDTDDGSGLHHMVYEVVDNAIDEALAGYATRVEVVLNA
DNSVTVRDDGRGIPVGIHKDEGVSAAEVIMTQLHAGGKFDQNSYKVSGGLHGVGVSVVNALSSKLELRIWREDKEHLIEF
AHGDAVAPLRVVGESKGKRGTEVTFLASTETFKMVEYDYATLEHRLRELAFLNSGVHIILSDMRHAVEKREEMFYSGGVE
EFVKYLDRNKKAIVPNPIMVRSEANGITVEAALWWNDSYHENVLCFTNNIPQRDGGTHLAGFRGALTRQVNGYAEANAKK
EKIALTGDDCREGLTAVLSVKVPDPKFSSQTKDKLVSSEVRPVVENVINEALSAWFEEHPSEAKTVVGKVIQAAAAREAA
RKARELTRKNPLNNIASLPGKLADCQEKDPAKSELFIVEGDSAGGSAKQGRNREFQAVLPLRGKILNVERVRPDKMLSSE
QIGTLITALGTGISDDFSIDKLRYHKIIVMTDADVDGAHIRTLLLTFFYRQMRQIIDRGHLYIAQPPLYKVTRGKSEQYL
KDERALEDYLIGTGLDDCVFRTADGEERKGRDLLALVEEARLVRNILRNLHSRYDRKVVEQAAIAGVLRGDITRDIATAD
AAAEYIAKRLDVLAEEVERGWIGHFVEGQGFTFERTVRGVKDVAVIDDALLASADARKLDDYAARLQDAYAKPGALRRGD
TQTVIHGPVDLFEAVTDSGRKGIAMQRYKGLGEMNPQQLWETTLDTEARTLLQVKVKEVDEADDIFTKLMGDVVEPRREF
IQDNSLSANVDV
>Mature_811_residues
TEPARQTIADNEPATANEYGAESIRVLKGLDAVRKRPGMYIGDTDDGSGLHHMVYEVVDNAIDEALAGYATRVEVVLNAD
NSVTVRDDGRGIPVGIHKDEGVSAAEVIMTQLHAGGKFDQNSYKVSGGLHGVGVSVVNALSSKLELRIWREDKEHLIEFA
HGDAVAPLRVVGESKGKRGTEVTFLASTETFKMVEYDYATLEHRLRELAFLNSGVHIILSDMRHAVEKREEMFYSGGVEE
FVKYLDRNKKAIVPNPIMVRSEANGITVEAALWWNDSYHENVLCFTNNIPQRDGGTHLAGFRGALTRQVNGYAEANAKKE
KIALTGDDCREGLTAVLSVKVPDPKFSSQTKDKLVSSEVRPVVENVINEALSAWFEEHPSEAKTVVGKVIQAAAAREAAR
KARELTRKNPLNNIASLPGKLADCQEKDPAKSELFIVEGDSAGGSAKQGRNREFQAVLPLRGKILNVERVRPDKMLSSEQ
IGTLITALGTGISDDFSIDKLRYHKIIVMTDADVDGAHIRTLLLTFFYRQMRQIIDRGHLYIAQPPLYKVTRGKSEQYLK
DERALEDYLIGTGLDDCVFRTADGEERKGRDLLALVEEARLVRNILRNLHSRYDRKVVEQAAIAGVLRGDITRDIATADA
AAEYIAKRLDVLAEEVERGWIGHFVEGQGFTFERTVRGVKDVAVIDDALLASADARKLDDYAARLQDAYAKPGALRRGDT
QTVIHGPVDLFEAVTDSGRKGIAMQRYKGLGEMNPQQLWETTLDTEARTLLQVKVKEVDEADDIFTKLMGDVVEPRREFI
QDNSLSANVDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=550, Percent_Identity=24.3636363636364, Blast_Score=121, Evalue=3e-27,
Organism=Homo sapiens, GI19913408, Length=552, Percent_Identity=24.8188405797101, Blast_Score=119, Evalue=1e-26,
Organism=Escherichia coli, GI48994957, Length=813, Percent_Identity=55.719557195572, Blast_Score=839, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=556, Percent_Identity=40.8273381294964, Blast_Score=394, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17535065, Length=534, Percent_Identity=25.2808988764045, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI212645845, Length=509, Percent_Identity=21.6110019646365, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI212645657, Length=172, Percent_Identity=30.8139534883721, Blast_Score=74, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6324241, Length=590, Percent_Identity=25.7627118644068, Blast_Score=126, Evalue=1e-29,
Organism=Drosophila melanogaster, GI17136538, Length=530, Percent_Identity=25.6603773584906, Blast_Score=127, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 89878; Mature: 89747

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEPARQTIADNEPATANEYGAESIRVLKGLDAVRKRPGMYIGDTDDGSGLHHMVYEVVD
CCCCHHHHHCCCCCCCCCHHCHHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHH
NAIDEALAGYATRVEVVLNADNSVTVRDDGRGIPVGIHKDEGVSAAEVIMTQLHAGGKFD
HHHHHHHHCCCEEEEEEEECCCEEEEEECCCCCCEEECCCCCCCHHHHHHHHHHCCCCCC
QNSYKVSGGLHGVGVSVVNALSSKLELRIWREDKEHLIEFAHGDAVAPLRVVGESKGKRG
CCCEEECCCCCHHHHHHHHHHHHHHEEEEECCCHHHHHHHHCCCCCCHHHEECCCCCCCC
TEVTFLASTETFKMVEYDYATLEHRLRELAFLNSGVHIILSDMRHAVEKREEMFYSGGVE
CEEEEEECCCEEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHH
EFVKYLDRNKKAIVPNPIMVRSEANGITVEAALWWNDSYHENVLCFTNNIPQRDGGTHLA
HHHHHHCCCCCEECCCCEEEEECCCCEEEEEEEEECCCCCCCEEEEECCCCCCCCCCCCH
GFRGALTRQVNGYAEANAKKEKIALTGDDCREGLTAVLSVKVPDPKFSSQTKDKLVSSEV
HHHHHHHHHHCCHHHCCCCCCEEEECCHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHH
RPVVENVINEALSAWFEEHPSEAKTVVGKVIQAAAAREAARKARELTRKNPLNNIASLPG
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCC
KLADCQEKDPAKSELFIVEGDSAGGSAKQGRNREFQAVLPLRGKILNVERVRPDKMLSSE
CHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCCCEEEEHHHCCHHHHHHHH
QIGTLITALGTGISDDFSIDKLRYHKIIVMTDADVDGAHIRTLLLTFFYRQMRQIIDRGH
HHHHHHHHHCCCCCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCC
LYIAQPPLYKVTRGKSEQYLKDERALEDYLIGTGLDDCVFRTADGEERKGRDLLALVEEA
EEEECCCCEEHHCCCCHHHHHHHHHHHHHHHCCCCHHHEEECCCCCCCCCHHHHHHHHHH
RLVRNILRNLHSRYDRKVVEQAAIAGVLRGDITRDIATADAAAEYIAKRLDVLAEEVERG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
WIGHFVEGQGFTFERTVRGVKDVAVIDDALLASADARKLDDYAARLQDAYAKPGALRRGD
CCCEEECCCCCCHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCC
TQTVIHGPVDLFEAVTDSGRKGIAMQRYKGLGEMNPQQLWETTLDTEARTLLQVKVKEVD
CCEEEECHHHHHHHHHCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
EADDIFTKLMGDVVEPRREFIQDNSLSANVDV
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TEPARQTIADNEPATANEYGAESIRVLKGLDAVRKRPGMYIGDTDDGSGLHHMVYEVVD
CCCHHHHHCCCCCCCCCHHCHHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHH
NAIDEALAGYATRVEVVLNADNSVTVRDDGRGIPVGIHKDEGVSAAEVIMTQLHAGGKFD
HHHHHHHHCCCEEEEEEEECCCEEEEEECCCCCCEEECCCCCCCHHHHHHHHHHCCCCCC
QNSYKVSGGLHGVGVSVVNALSSKLELRIWREDKEHLIEFAHGDAVAPLRVVGESKGKRG
CCCEEECCCCCHHHHHHHHHHHHHHEEEEECCCHHHHHHHHCCCCCCHHHEECCCCCCCC
TEVTFLASTETFKMVEYDYATLEHRLRELAFLNSGVHIILSDMRHAVEKREEMFYSGGVE
CEEEEEECCCEEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHH
EFVKYLDRNKKAIVPNPIMVRSEANGITVEAALWWNDSYHENVLCFTNNIPQRDGGTHLA
HHHHHHCCCCCEECCCCEEEEECCCCEEEEEEEEECCCCCCCEEEEECCCCCCCCCCCCH
GFRGALTRQVNGYAEANAKKEKIALTGDDCREGLTAVLSVKVPDPKFSSQTKDKLVSSEV
HHHHHHHHHHCCHHHCCCCCCEEEECCHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHH
RPVVENVINEALSAWFEEHPSEAKTVVGKVIQAAAAREAARKARELTRKNPLNNIASLPG
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCC
KLADCQEKDPAKSELFIVEGDSAGGSAKQGRNREFQAVLPLRGKILNVERVRPDKMLSSE
CHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCCCEEEEHHHCCHHHHHHHH
QIGTLITALGTGISDDFSIDKLRYHKIIVMTDADVDGAHIRTLLLTFFYRQMRQIIDRGH
HHHHHHHHHCCCCCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCC
LYIAQPPLYKVTRGKSEQYLKDERALEDYLIGTGLDDCVFRTADGEERKGRDLLALVEEA
EEEECCCCEEHHCCCCHHHHHHHHHHHHHHHCCCCHHHEEECCCCCCCCCHHHHHHHHHH
RLVRNILRNLHSRYDRKVVEQAAIAGVLRGDITRDIATADAAAEYIAKRLDVLAEEVERG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
WIGHFVEGQGFTFERTVRGVKDVAVIDDALLASADARKLDDYAARLQDAYAKPGALRRGD
CCCEEECCCCCCHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCC
TQTVIHGPVDLFEAVTDSGRKGIAMQRYKGLGEMNPQQLWETTLDTEARTLLQVKVKEVD
CCEEEECHHHHHHHHHCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
EADDIFTKLMGDVVEPRREFIQDNSLSANVDV
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8226640 [H]