Click here to switch to the map view.

The map label for this gene is norM [H]

Identifier: 146302752

GI number: 146302752

Start: 6063510

End: 6064895

Strand: Reverse

Name: norM [H]

Synonym: Fjoh_5025

Alternate gene names: 146302752

Gene position: 6064895-6063510 (Counterclockwise)

Preceding gene: 146302755

Following gene: 146302751

Centisome position: 99.48

GC content: 35.43

Gene sequence:

>1386_bases
GTGAATTTAAAGCAGTACACAAAAGAATTTTCATATAATTTTAAACTTGCCTATCCTGTTATTTTAGGAATGGTTGGCCA
TACTTTAATTGGTATTGTCGACAATATAATGGTAGGGAAAATTGGCAGTACAGAATTAGCAGCAGTTTCATTAGGAAACA
GCATGATTTTTATTGCAATGTCTCTGGGAATTGGTTTTTCTACTGCCATAACTCCAATTGTAGCCGAAGGCGATGCAGAA
AAAAATGACAGTAAAATTCGTTCTGCTTTTCACCACGGATTATTTTTATGCACCATTTTAGGACTAGTGCTTTTTACTGT
AATCATGTTTGCAAAACCTATAATGGAATTATTAAAACAGCCTGCAGATGTTATTGTTCTGGCAAAACCTTATTTGGGCT
GGGTAGCTTTTTCGTTGATTCCTTTAATTATGTATCAGGGATATAAGCAATTTGCAGACGGAATGTCTCTTACCAAATAT
TCTATGTATGCCATGGTTATGGCAAACGTGCTGCACGTAGGGTTAAACTATGTTTTAATTTACGGAATCTGGATTTTTCC
AAAAATGGGAATTATTGGGGCGGCATTGGGAACTGTGATTTCGAGAATATTTTTGGTAATGTTTATGCATATAATGTTGT
CAAGAAGAGACGATTTAAAACGTTTCTTTAAAGGTTTCAGTTTTGATGAAATCAAAAAAGCAACGATCAAAAAAATCATC
AGCATTGGATTTCCATCGGCAATGCAAATGTTGTTTGAAGTAGTTTTGTTTACCGCTTCTATATGGCTTTGCGGTAATAT
TGGAAAAACCAGTCAGGCCGCTAATCAGATTGCTTTAAGTCTGGCTTCGATGACTTTTATGTTTGCAATGGGATTGAGCG
TCACTTCGATGATCAGGGTCAGCAACCAAAGAGGATTAAACGATTATAAAAAGCTGATTGTCGTAGCCCGTTCTATTTTC
TTACTGGCAATTATACTCGAAACTTTCTTTGCACTTCTATTTATAGTATTCCATAATTATCTGCCGCATATGTTTTTAAA
TATGGAAAATACAGGACAGGTTCTGGATAATACCGAAGTAATTGCAATTGCATCAAAACTGCTTTTAATTGCGGCTGTTT
TCCAAATTTCTGATGGAATTCAGGTTGTAGTTTTGGGCGCTCTGCGTGGCTTACAGGATGTAAAAGTGCCTATGTATATT
ACATTTGTGGCTTATTGGATCATTGGTTTTCCTATTTCATATTATTTAGGAGAGCATACAGAACTAAAAGCGCAAGGCGT
TTGGATAGGACTTTTGGCAGGTTTAACAGCAGCAGCAATTTTTCTGTATATTCGCTTTCATTTTCTAACGAAGAAGTTAA
TCGCAAATTCACCTTTAAATAATTAA

Upstream 100 bases:

>100_bases
TATGGTATGCTGATTTTAGTCTTAATATTGGCTTATGTTTTTATAAATAGATCAGAATTCGAAGCTTCTGATTATCTTTG
CGCACTAAAAAAGAATACAA

Downstream 100 bases:

>100_bases
GTTTGAAGCTTGAATAATAATATAAAACAGATTTTAGGATTGAAAAATCATTTTAATCTGTAACAAAAAACGAGTCTGAT
AACAGACAATTGTAACATCT

Product: MATE efflux family protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter; Na(+)/drug antiporter [H]

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MNLKQYTKEFSYNFKLAYPVILGMVGHTLIGIVDNIMVGKIGSTELAAVSLGNSMIFIAMSLGIGFSTAITPIVAEGDAE
KNDSKIRSAFHHGLFLCTILGLVLFTVIMFAKPIMELLKQPADVIVLAKPYLGWVAFSLIPLIMYQGYKQFADGMSLTKY
SMYAMVMANVLHVGLNYVLIYGIWIFPKMGIIGAALGTVISRIFLVMFMHIMLSRRDDLKRFFKGFSFDEIKKATIKKII
SIGFPSAMQMLFEVVLFTASIWLCGNIGKTSQAANQIALSLASMTFMFAMGLSVTSMIRVSNQRGLNDYKKLIVVARSIF
LLAIILETFFALLFIVFHNYLPHMFLNMENTGQVLDNTEVIAIASKLLLIAAVFQISDGIQVVVLGALRGLQDVKVPMYI
TFVAYWIIGFPISYYLGEHTELKAQGVWIGLLAGLTAAAIFLYIRFHFLTKKLIANSPLNN

Sequences:

>Translated_461_residues
MNLKQYTKEFSYNFKLAYPVILGMVGHTLIGIVDNIMVGKIGSTELAAVSLGNSMIFIAMSLGIGFSTAITPIVAEGDAE
KNDSKIRSAFHHGLFLCTILGLVLFTVIMFAKPIMELLKQPADVIVLAKPYLGWVAFSLIPLIMYQGYKQFADGMSLTKY
SMYAMVMANVLHVGLNYVLIYGIWIFPKMGIIGAALGTVISRIFLVMFMHIMLSRRDDLKRFFKGFSFDEIKKATIKKII
SIGFPSAMQMLFEVVLFTASIWLCGNIGKTSQAANQIALSLASMTFMFAMGLSVTSMIRVSNQRGLNDYKKLIVVARSIF
LLAIILETFFALLFIVFHNYLPHMFLNMENTGQVLDNTEVIAIASKLLLIAAVFQISDGIQVVVLGALRGLQDVKVPMYI
TFVAYWIIGFPISYYLGEHTELKAQGVWIGLLAGLTAAAIFLYIRFHFLTKKLIANSPLNN
>Mature_461_residues
MNLKQYTKEFSYNFKLAYPVILGMVGHTLIGIVDNIMVGKIGSTELAAVSLGNSMIFIAMSLGIGFSTAITPIVAEGDAE
KNDSKIRSAFHHGLFLCTILGLVLFTVIMFAKPIMELLKQPADVIVLAKPYLGWVAFSLIPLIMYQGYKQFADGMSLTKY
SMYAMVMANVLHVGLNYVLIYGIWIFPKMGIIGAALGTVISRIFLVMFMHIMLSRRDDLKRFFKGFSFDEIKKATIKKII
SIGFPSAMQMLFEVVLFTASIWLCGNIGKTSQAANQIALSLASMTFMFAMGLSVTSMIRVSNQRGLNDYKKLIVVARSIF
LLAIILETFFALLFIVFHNYLPHMFLNMENTGQVLDNTEVIAIASKLLLIAAVFQISDGIQVVVLGALRGLQDVKVPMYI
TFVAYWIIGFPISYYLGEHTELKAQGVWIGLLAGLTAAAIFLYIRFHFLTKKLIANSPLNN

Specific function: Multidrug efflux pump that functions as a Na(+)/drug antiporter. Confers resistance to norfloxacin, ciprofloxacin, ofloxacin, daunomycin, doxorubicin, streptomycin, kanamycin, ethidium bromide and acriflavine [H]

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family [H]

Homologues:

Organism=Homo sapiens, GI153792564, Length=421, Percent_Identity=23.0403800475059, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI22907060, Length=438, Percent_Identity=22.1461187214612, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI38261960, Length=453, Percent_Identity=22.2958057395143, Blast_Score=87, Evalue=4e-17,
Organism=Escherichia coli, GI48994890, Length=460, Percent_Identity=30.6521739130435, Blast_Score=189, Evalue=4e-49,
Organism=Escherichia coli, GI226510954, Length=466, Percent_Identity=23.8197424892704, Blast_Score=94, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6321821, Length=400, Percent_Identity=20.5, Blast_Score=67, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6320545, Length=452, Percent_Identity=21.2389380530973, Blast_Score=64, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528 [H]

Pfam domain/function: PF01554 MatE [H]

EC number: NA

Molecular weight: Translated: 51207; Mature: 51207

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLKQYTKEFSYNFKLAYPVILGMVGHTLIGIVDNIMVGKIGSTELAAVSLGNSMIFIAM
CCHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHEECCCCCEEHHH
SLGIGFSTAITPIVAEGDAEKNDSKIRSAFHHGLFLCTILGLVLFTVIMFAKPIMELLKQ
HHCCCHHHHHCHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PADVIVLAKPYLGWVAFSLIPLIMYQGYKQFADGMSLTKYSMYAMVMANVLHVGLNYVLI
CCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
YGIWIFPKMGIIGAALGTVISRIFLVMFMHIMLSRRDDLKRFFKGFSFDEIKKATIKKII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHHHH
SIGFPSAMQMLFEVVLFTASIWLCGNIGKTSQAANQIALSLASMTFMFAMGLSVTSMIRV
HCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SNQRGLNDYKKLIVVARSIFLLAIILETFFALLFIVFHNYLPHMFLNMENTGQVLDNTEV
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHCCCHH
IAIASKLLLIAAVFQISDGIQVVVLGALRGLQDVKVPMYITFVAYWIIGFPISYYLGEHT
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHCHHHCCHHHHHHHHHHHHHHHHHHHHHCCCC
ELKAQGVWIGLLAGLTAAAIFLYIRFHFLTKKLIANSPLNN
CCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNLKQYTKEFSYNFKLAYPVILGMVGHTLIGIVDNIMVGKIGSTELAAVSLGNSMIFIAM
CCHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHEECCCCCEEHHH
SLGIGFSTAITPIVAEGDAEKNDSKIRSAFHHGLFLCTILGLVLFTVIMFAKPIMELLKQ
HHCCCHHHHHCHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PADVIVLAKPYLGWVAFSLIPLIMYQGYKQFADGMSLTKYSMYAMVMANVLHVGLNYVLI
CCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
YGIWIFPKMGIIGAALGTVISRIFLVMFMHIMLSRRDDLKRFFKGFSFDEIKKATIKKII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHHHH
SIGFPSAMQMLFEVVLFTASIWLCGNIGKTSQAANQIALSLASMTFMFAMGLSVTSMIRV
HCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SNQRGLNDYKKLIVVARSIFLLAIILETFFALLFIVFHNYLPHMFLNMENTGQVLDNTEV
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHCCCHH
IAIASKLLLIAAVFQISDGIQVVVLGALRGLQDVKVPMYITFVAYWIIGFPISYYLGEHT
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHCHHHCCHHHHHHHHHHHHHHHHHHHHHCCCC
ELKAQGVWIGLLAGLTAAAIFLYIRFHFLTKKLIANSPLNN
CCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11583854; 10952301 [H]