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The map label for this gene is wcaJ [H]

Identifier: 146302721

GI number: 146302721

Start: 6030601

End: 6032013

Strand: Reverse

Name: wcaJ [H]

Synonym: Fjoh_4994

Alternate gene names: 146302721

Gene position: 6032013-6030601 (Counterclockwise)

Preceding gene: 146302727

Following gene: 146302719

Centisome position: 98.94

GC content: 33.05

Gene sequence:

>1413_bases
ATGAGAATTTTAGAAGAACTGTCAAGATTTAGAATGTCCAGATATTTCAAACTTTTGTTTGTATGCTGGGATGTCATTTT
GTTAAATACTGCGATACTGCTTTCTTCTTTAGCCAGATTTGGAAGTTTAGACGAACTTTTAATAAAAGAAGAAAAAACAG
TTTCTGTTCTGGCTAACTTAATTTGGATAGGACTATTGCTAAATAAGGATTCATACAGAATGATTCGTATTGAACGTATT
GAATCTATTTTAAAACGAACCATTAGAAAAGTAGTTATTCATGCTTCGATAATTTCAATTATTGTAGTATATCTAAAATT
CTCTGATCTTTCCAGACTTCGACTGTTATATTTCTATTTGTTCTTTTTTGTGATTTTATTGGTTTCCAGATATATTTCAA
TGAAACTTTTAAAAGAAATTAGAGCTAAAGGGTATAATTTTAGAAAAATTATTATCGTAGGGGCAAATGAAACGGGCGAA
AGAATGCGAAAAATTTTGGCTAAAGATTTGACTTACGGCTATAAGTTTTTGGGTTTTTTTGATGAAAAAGCCGATCCTAT
TTTTTCAGATCAGGTAATTGGCGGTTTTGATGAAATTCAGGATTTTATAGTTCAAAAAGAAGTAGACGAAATGTACGTTG
CCCTGCATATAGATGATATTGCGGTAATCAATAAACTGGTTAAAATCTGTGAACAAAATATGGTGAGAATTAAATTTGTT
CCTGATTTTCAGTTGTACACAAAATCAAGTAAAGTCGAAATTACTTTTTATGAAAATACTCCGGTATTAATGCTCCGCCG
AGAACCTTTAGAGTTTGTTACCAATAAATTGATAAAAAAAGCTTTTGATGTTTGTTTTGCTGGCTTGGTCATTTTGCTGA
TATTTCCGTGGCTTTTTCCAATTGTAATGCTGATTATAAAATTAGAATCGCCAGGTCCTGTTTTCTTTAGGCAAAAACGT
TCAGGACGAGACAACAGACCTTTTATTTGTCTAAAATTCAGAAGCATGTATGTTAACGATCTTGCGCACAATAAGCAGGC
AGGAAAAGGAGACAGCCGCGTAACAAAATTTGGAGCTTTTATTCGTAAAACCAGTATTGATGAACTGCCGCAGTTTTTTA
ATGTATTTTGGGGAGATATGTCAGTAGTTGGACCAAGGCCGCACATGATAAATCTGGCCAAAGAATACAGCGAATTGATT
AATAATTATTTAGTTCGCCAGTTTGCAAAACCAGGTATTACCGGCTGGGCACAAGTCAACGGTTATCGAGGCGAGACAAA
AGAACTTTCTGATATGGAAAATAGAGTAGAATATGATATTTGGTATATCGAAAACTGGAGTTTACTTCTCGATATCAAAA
TCATAGTGAGAACTGTAATAAACATTCTTAAAGGTGATGAAAACGCCTATTAG

Upstream 100 bases:

>100_bases
GTAAAATTTTTAAAGATTAGCTGAAAAAATAAGATTATTAATATAAATAAAATGTTATTTTGCACCTGTAAGTTTAGCTT
TAAACTGAGTTTCCCCAAAT

Downstream 100 bases:

>100_bases
AATTTAAAAAACTGATTATTAGCAATAACTTCTTTTAAGTTTGAAGCCGCATCTATTTTGATTTTTTGATGCATAGATTC
TATATGACTGCTGTGATGCA

Product: undecaprenyl-phosphate galactose phosphotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MRILEELSRFRMSRYFKLLFVCWDVILLNTAILLSSLARFGSLDELLIKEEKTVSVLANLIWIGLLLNKDSYRMIRIERI
ESILKRTIRKVVIHASIISIIVVYLKFSDLSRLRLLYFYLFFFVILLVSRYISMKLLKEIRAKGYNFRKIIIVGANETGE
RMRKILAKDLTYGYKFLGFFDEKADPIFSDQVIGGFDEIQDFIVQKEVDEMYVALHIDDIAVINKLVKICEQNMVRIKFV
PDFQLYTKSSKVEITFYENTPVLMLRREPLEFVTNKLIKKAFDVCFAGLVILLIFPWLFPIVMLIIKLESPGPVFFRQKR
SGRDNRPFICLKFRSMYVNDLAHNKQAGKGDSRVTKFGAFIRKTSIDELPQFFNVFWGDMSVVGPRPHMINLAKEYSELI
NNYLVRQFAKPGITGWAQVNGYRGETKELSDMENRVEYDIWYIENWSLLLDIKIIVRTVINILKGDENAY

Sequences:

>Translated_470_residues
MRILEELSRFRMSRYFKLLFVCWDVILLNTAILLSSLARFGSLDELLIKEEKTVSVLANLIWIGLLLNKDSYRMIRIERI
ESILKRTIRKVVIHASIISIIVVYLKFSDLSRLRLLYFYLFFFVILLVSRYISMKLLKEIRAKGYNFRKIIIVGANETGE
RMRKILAKDLTYGYKFLGFFDEKADPIFSDQVIGGFDEIQDFIVQKEVDEMYVALHIDDIAVINKLVKICEQNMVRIKFV
PDFQLYTKSSKVEITFYENTPVLMLRREPLEFVTNKLIKKAFDVCFAGLVILLIFPWLFPIVMLIIKLESPGPVFFRQKR
SGRDNRPFICLKFRSMYVNDLAHNKQAGKGDSRVTKFGAFIRKTSIDELPQFFNVFWGDMSVVGPRPHMINLAKEYSELI
NNYLVRQFAKPGITGWAQVNGYRGETKELSDMENRVEYDIWYIENWSLLLDIKIIVRTVINILKGDENAY
>Mature_470_residues
MRILEELSRFRMSRYFKLLFVCWDVILLNTAILLSSLARFGSLDELLIKEEKTVSVLANLIWIGLLLNKDSYRMIRIERI
ESILKRTIRKVVIHASIISIIVVYLKFSDLSRLRLLYFYLFFFVILLVSRYISMKLLKEIRAKGYNFRKIIIVGANETGE
RMRKILAKDLTYGYKFLGFFDEKADPIFSDQVIGGFDEIQDFIVQKEVDEMYVALHIDDIAVINKLVKICEQNMVRIKFV
PDFQLYTKSSKVEITFYENTPVLMLRREPLEFVTNKLIKKAFDVCFAGLVILLIFPWLFPIVMLIIKLESPGPVFFRQKR
SGRDNRPFICLKFRSMYVNDLAHNKQAGKGDSRVTKFGAFIRKTSIDELPQFFNVFWGDMSVVGPRPHMINLAKEYSELI
NNYLVRQFAKPGITGWAQVNGYRGETKELSDMENRVEYDIWYIENWSLLLDIKIIVRTVINILKGDENAY

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=347, Percent_Identity=38.6167146974063, Blast_Score=234, Evalue=1e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 55114; Mature: 55114

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILEELSRFRMSRYFKLLFVCWDVILLNTAILLSSLARFGSLDELLIKEEKTVSVLANL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHH
IWIGLLLNKDSYRMIRIERIESILKRTIRKVVIHASIISIIVVYLKFSDLSRLRLLYFYL
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
FFFVILLVSRYISMKLLKEIRAKGYNFRKIIIVGANETGERMRKILAKDLTYGYKFLGFF
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
DEKADPIFSDQVIGGFDEIQDFIVQKEVDEMYVALHIDDIAVINKLVKICEQNMVRIKFV
CCCCCCCCCCHHCCCHHHHHHHHHHHHHHHEEEEEEEHHHHHHHHHHHHHHCCCEEEEEE
PDFQLYTKSSKVEITFYENTPVLMLRREPLEFVTNKLIKKAFDVCFAGLVILLIFPWLFP
CCHHEEECCCEEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVMLIIKLESPGPVFFRQKRSGRDNRPFICLKFRSMYVNDLAHNKQAGKGDSRVTKFGAF
HHHHHHCCCCCCCCEEEECCCCCCCCCEEEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHH
IRKTSIDELPQFFNVFWGDMSVVGPRPHMINLAKEYSELINNYLVRQFAKPGITGWAQVN
HHHCCHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEC
GYRGETKELSDMENRVEYDIWYIENWSLLLDIKIIVRTVINILKGDENAY
CCCCCCHHHHHHHHCCEEEEEEEECCEEEEHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRILEELSRFRMSRYFKLLFVCWDVILLNTAILLSSLARFGSLDELLIKEEKTVSVLANL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHH
IWIGLLLNKDSYRMIRIERIESILKRTIRKVVIHASIISIIVVYLKFSDLSRLRLLYFYL
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
FFFVILLVSRYISMKLLKEIRAKGYNFRKIIIVGANETGERMRKILAKDLTYGYKFLGFF
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
DEKADPIFSDQVIGGFDEIQDFIVQKEVDEMYVALHIDDIAVINKLVKICEQNMVRIKFV
CCCCCCCCCCHHCCCHHHHHHHHHHHHHHHEEEEEEEHHHHHHHHHHHHHHCCCEEEEEE
PDFQLYTKSSKVEITFYENTPVLMLRREPLEFVTNKLIKKAFDVCFAGLVILLIFPWLFP
CCHHEEECCCEEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVMLIIKLESPGPVFFRQKRSGRDNRPFICLKFRSMYVNDLAHNKQAGKGDSRVTKFGAF
HHHHHHCCCCCCCCEEEECCCCCCCCCEEEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHH
IRKTSIDELPQFFNVFWGDMSVVGPRPHMINLAKEYSELINNYLVRQFAKPGITGWAQVN
HHHCCHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEC
GYRGETKELSDMENRVEYDIWYIENWSLLLDIKIIVRTVINILKGDENAY
CCCCCCHHHHHHHHCCEEEEEEEECCEEEEHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]