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The map label for this gene is emrA [H]

Identifier: 146302630

GI number: 146302630

Start: 5924585

End: 5925664

Strand: Direct

Name: emrA [H]

Synonym: Fjoh_4903

Alternate gene names: 146302630

Gene position: 5924585-5925664 (Clockwise)

Preceding gene: 146302629

Following gene: 146302631

Centisome position: 97.17

GC content: 33.98

Gene sequence:

>1080_bases
ATGGAAAAGAAAAAGACAAATAAAAAATTCATCATCATACTTGCAGTTCTGATTTTAGGAGGCGGAACTTATGGAATCTC
TAAATATTTACATTCACAAGCTCACGAAGAAACAGATGATGCTCAGATTGAGAAAAAAATGAACCCAATTATCCCGAGAG
TTTCAGGATATATTAGTAAAGTGTATGTGAAAGATAATGACTATGTAAAAAAAGGAGATACTTTATTTACTATCGATAAG
AGAGATTATCAATTAAAAATCGATGAAGCAAATGCTGCATTATTAGGAGCTGAAGGTCAATTTGAAGCAGCAAAAGCTGA
TATTGGAAGTGCAAATGCAAGCATTTCAGTATCTGACGCTCAAATGAGATCTGCATCTGGTTCTATCGAAAGTGCAAAAA
TCAGATTAAGACAGCTTACAAACGATTTTAACCGTTATAACAATTTATACAAAACTCATACAATTACAAAACAGCAATTC
GAGCAGGCTCAGGCTGCAAAAGATGAAGCTGAAAATCAAGTACGTGTTTTAGAACAGCAGCAAAGAGCAAGCTCTTACCA
AAAATCAGTTATTCAGTCAAAATCTAAAGTTTCTGATAAGCAGACAGAAGTTGCAGCAGCAAACATCAAAAAAGCAAAAA
CAATGCTTGATGTTGCTCACTTAAACCTTTCTTATACAGTAGTTACAGCTGCTATTGACGGTCAGGTTTCTAAAGTAGAT
ATTCAGCCGGGACAATTGGTGCAGCCAGGACAATCATTATTCTATATCATTAATAATAATGAAGCCTGGGTTGTAGCTAA
CTTTAAAGAAACACAATTAAACAAAATGGTTGTGGGACAAAAAGTAGGTTTAAAAGTTGATGCTTATCCAAACTATGAGT
TTAAAGGAACTGTATCTTCTTTCTCTCCTGCAACAGGATCTCGTTTTTCATTGTTACCTCCTGATAATGCAACAGGAAAC
TTCGTAAAAACAATTCAGAGATTACCAGTAAAAATTACTATAGACGAATCTAACGATCCTGAAAAAGTAAAACTTTTAAG
ACCAGGAATGAACGTTGACGTTGATGTACATTTAAAATAA

Upstream 100 bases:

>100_bases
AGCAAAAGCAACAATTATTCAAAAATATTACGAATTACTTTCAGTATCAGGACAATTATCACAATCATTCAATCTTTCTA
AAATATAATCGATAGCTCTC

Downstream 100 bases:

>100_bases
AACATGGCAGGAGCAGTACAAGCAGACGACGATTTAGTAGAATACGGTTTCAGACGTGTTATCATTACGATTACAGCAGT
GCTTTGTGCACTGTTGGAAA

Product: secretion protein HlyD family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MEKKKTNKKFIIILAVLILGGGTYGISKYLHSQAHEETDDAQIEKKMNPIIPRVSGYISKVYVKDNDYVKKGDTLFTIDK
RDYQLKIDEANAALLGAEGQFEAAKADIGSANASISVSDAQMRSASGSIESAKIRLRQLTNDFNRYNNLYKTHTITKQQF
EQAQAAKDEAENQVRVLEQQQRASSYQKSVIQSKSKVSDKQTEVAAANIKKAKTMLDVAHLNLSYTVVTAAIDGQVSKVD
IQPGQLVQPGQSLFYIINNNEAWVVANFKETQLNKMVVGQKVGLKVDAYPNYEFKGTVSSFSPATGSRFSLLPPDNATGN
FVKTIQRLPVKITIDESNDPEKVKLLRPGMNVDVDVHLK

Sequences:

>Translated_359_residues
MEKKKTNKKFIIILAVLILGGGTYGISKYLHSQAHEETDDAQIEKKMNPIIPRVSGYISKVYVKDNDYVKKGDTLFTIDK
RDYQLKIDEANAALLGAEGQFEAAKADIGSANASISVSDAQMRSASGSIESAKIRLRQLTNDFNRYNNLYKTHTITKQQF
EQAQAAKDEAENQVRVLEQQQRASSYQKSVIQSKSKVSDKQTEVAAANIKKAKTMLDVAHLNLSYTVVTAAIDGQVSKVD
IQPGQLVQPGQSLFYIINNNEAWVVANFKETQLNKMVVGQKVGLKVDAYPNYEFKGTVSSFSPATGSRFSLLPPDNATGN
FVKTIQRLPVKITIDESNDPEKVKLLRPGMNVDVDVHLK
>Mature_359_residues
MEKKKTNKKFIIILAVLILGGGTYGISKYLHSQAHEETDDAQIEKKMNPIIPRVSGYISKVYVKDNDYVKKGDTLFTIDK
RDYQLKIDEANAALLGAEGQFEAAKADIGSANASISVSDAQMRSASGSIESAKIRLRQLTNDFNRYNNLYKTHTITKQQF
EQAQAAKDEAENQVRVLEQQQRASSYQKSVIQSKSKVSDKQTEVAAANIKKAKTMLDVAHLNLSYTVVTAAIDGQVSKVD
IQPGQLVQPGQSLFYIINNNEAWVVANFKETQLNKMVVGQKVGLKVDAYPNYEFKGTVSSFSPATGSRFSLLPPDNATGN
FVKTIQRLPVKITIDESNDPEKVKLLRPGMNVDVDVHLK

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789041, Length=326, Percent_Identity=32.8220858895706, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1788711, Length=337, Percent_Identity=31.7507418397626, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1790519, Length=316, Percent_Identity=28.7974683544304, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1787013, Length=302, Percent_Identity=25.1655629139073, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790012, Length=292, Percent_Identity=24.6575342465753, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790024, Length=289, Percent_Identity=24.5674740484429, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 39779; Mature: 39779

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKKKTNKKFIIILAVLILGGGTYGISKYLHSQAHEETDDAQIEKKMNPIIPRVSGYISK
CCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHCCCEEE
VYVKDNDYVKKGDTLFTIDKRDYQLKIDEANAALLGAEGQFEAAKADIGSANASISVSDA
EEECCCCEEECCCEEEEEECCCEEEEEECCCEEEEECCCCCCHHHCCCCCCCCEEEECHH
QMRSASGSIESAKIRLRQLTNDFNRYNNLYKTHTITKQQFEQAQAAKDEAENQVRVLEQQ
HHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
QRASSYQKSVIQSKSKVSDKQTEVAAANIKKAKTMLDVAHLNLSYTVVTAAIDGQVSKVD
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCEEEEE
IQPGQLVQPGQSLFYIINNNEAWVVANFKETQLNKMVVGQKVGLKVDAYPNYEFKGTVSS
CCCCCCCCCCCEEEEEEECCCEEEEEECHHHHHHHHHCCCCCCEEEECCCCCEEEECCCC
FSPATGSRFSLLPPDNATGNFVKTIQRLPVKITIDESNDPEKVKLLRPGMNVDVDVHLK
CCCCCCCEEEECCCCCCCCHHHHHHHHCCEEEEECCCCCCCEEEEECCCCCCCEEEEEC
>Mature Secondary Structure
MEKKKTNKKFIIILAVLILGGGTYGISKYLHSQAHEETDDAQIEKKMNPIIPRVSGYISK
CCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHCCCEEE
VYVKDNDYVKKGDTLFTIDKRDYQLKIDEANAALLGAEGQFEAAKADIGSANASISVSDA
EEECCCCEEECCCEEEEEECCCEEEEEECCCEEEEECCCCCCHHHCCCCCCCCEEEECHH
QMRSASGSIESAKIRLRQLTNDFNRYNNLYKTHTITKQQFEQAQAAKDEAENQVRVLEQQ
HHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
QRASSYQKSVIQSKSKVSDKQTEVAAANIKKAKTMLDVAHLNLSYTVVTAAIDGQVSKVD
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCEEEEE
IQPGQLVQPGQSLFYIINNNEAWVVANFKETQLNKMVVGQKVGLKVDAYPNYEFKGTVSS
CCCCCCCCCCCEEEEEEECCCEEEEEECHHHHHHHHHCCCCCCEEEECCCCCEEEECCCC
FSPATGSRFSLLPPDNATGNFVKTIQRLPVKITIDESNDPEKVKLLRPGMNVDVDVHLK
CCCCCCCEEEECCCCCCCCHHHHHHHHCCEEEEECCCCCCCEEEEECCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1409590; 9205837; 9278503; 8203018 [H]