Click here to switch to the map view.

The map label for this gene is trpB [H]

Identifier: 146302623

GI number: 146302623

Start: 5917515

End: 5918696

Strand: Direct

Name: trpB [H]

Synonym: Fjoh_4896

Alternate gene names: 146302623

Gene position: 5917515-5918696 (Clockwise)

Preceding gene: 146302622

Following gene: 146302624

Centisome position: 97.06

GC content: 40.69

Gene sequence:

>1182_bases
ATGAGTTTTAACGTTAACGAAAAAGGATATTACGGGGAATTTGGAGGAGCTTACATTCCAGAAATGTTATATCCGAATGT
AGAAGAACTACGCCAAAAATACTTAAGCATAATGGACGAACCTGATTTTAAGGCAGAGTTCAACCAATTGCTTAAAGATT
ATGTAGGACGCCCTAGTCCGTTGTATTTTGCAAAACGCTTATCAGAAAAATACAATACAAAGGTTTATCTAAAAAGAGAA
GATTTAAACCATACCGGAGCGCATAAAGTCAACAACACAATCGGGCAGATTTTATTAGCAAAACGTTTAGGTAAAAAAAG
AATTATTGCAGAAACCGGTGCGGGTCAGCATGGTGTGGCAACTGCAACAGTCTGCGCACTAATGGGAATTGAATGCATCG
TTTATATGGGAGAAATTGACATTGCGCGTCAGGCACCAAACGTTGCGCGTATGAAAATGCTTGGTGCAGAAGTTCGTCCG
GCTCTTTCAGGTTCAAGAACCTTAAAAGATGCTACCAACGAAGCAATTCGTGATTGGATTAATAACCCTGTAGACACACA
TTATATCATTGGATCGGCAATTGGACCGCATCCTTACCCTGATATGGTAACACGTTTTCAGAGTATTATCTCAGAAGAAA
TAAAATGGCAGTTAAAAGAAAAAGAAGGACGCGAAAATCCTGATTATGTTGTTGCCTGCATTGGCGGCGGAAGTAACGCC
GCAGGAACCTATTATCACTTTCTGCACGAACCGCAAGTTGGAATTATTGCTGTTGAAGCAGCCGGAAAAGGTGTTGACAG
CGGGCACAGTGCTGCAACCAGTAAATTAGGAAAAGTCGGCGTTATTCACGGTTGTAAAACACTGTTAATGCAGACGCCAG
ACGGACAAATTACTGAGCCATATTCGATTTCTGCGGGACTTGATTATCCTGGAGTTGGGCCTTTGCACGCACATTTAGCA
CAAACCGGGCGCGGCGAATTTTTCTCTGTAACAGATGATGATGCTATGAATGCTGGTTTGCAGCTGACCAAACTAGAAGG
AATTATTCCTGCTATTGAAAGCGCTCATGCATTTGCAGTTTTGGATCAGAAAAAATTCAAACCAGAAGATATTGTAGTGA
TCAGTCTTTCAGGACGTGGAGATAAAGATTTAGATAATTATATTGATTACTTTAAATTGTAA

Upstream 100 bases:

>100_bases
TAAATAGTAAATTTGAAATTGAACCTGGGCTGAAAAACAGAAATTTATTTAGCAATTTCAAACGAAAATTTGATGTTGCC
AACTTTTAAAATTTAAAAAA

Downstream 100 bases:

>100_bases
TAAAAATTGTAATTTGGACGTTGGTTTTTAAAATCGCTAACGCAAACAATAACAAGAAAAATCATAATTATGGAACTATT
ATTTTCATACGGAACTTTAA

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MSFNVNEKGYYGEFGGAYIPEMLYPNVEELRQKYLSIMDEPDFKAEFNQLLKDYVGRPSPLYFAKRLSEKYNTKVYLKRE
DLNHTGAHKVNNTIGQILLAKRLGKKRIIAETGAGQHGVATATVCALMGIECIVYMGEIDIARQAPNVARMKMLGAEVRP
ALSGSRTLKDATNEAIRDWINNPVDTHYIIGSAIGPHPYPDMVTRFQSIISEEIKWQLKEKEGRENPDYVVACIGGGSNA
AGTYYHFLHEPQVGIIAVEAAGKGVDSGHSAATSKLGKVGVIHGCKTLLMQTPDGQITEPYSISAGLDYPGVGPLHAHLA
QTGRGEFFSVTDDDAMNAGLQLTKLEGIIPAIESAHAFAVLDQKKFKPEDIVVISLSGRGDKDLDNYIDYFKL

Sequences:

>Translated_393_residues
MSFNVNEKGYYGEFGGAYIPEMLYPNVEELRQKYLSIMDEPDFKAEFNQLLKDYVGRPSPLYFAKRLSEKYNTKVYLKRE
DLNHTGAHKVNNTIGQILLAKRLGKKRIIAETGAGQHGVATATVCALMGIECIVYMGEIDIARQAPNVARMKMLGAEVRP
ALSGSRTLKDATNEAIRDWINNPVDTHYIIGSAIGPHPYPDMVTRFQSIISEEIKWQLKEKEGRENPDYVVACIGGGSNA
AGTYYHFLHEPQVGIIAVEAAGKGVDSGHSAATSKLGKVGVIHGCKTLLMQTPDGQITEPYSISAGLDYPGVGPLHAHLA
QTGRGEFFSVTDDDAMNAGLQLTKLEGIIPAIESAHAFAVLDQKKFKPEDIVVISLSGRGDKDLDNYIDYFKL
>Mature_392_residues
SFNVNEKGYYGEFGGAYIPEMLYPNVEELRQKYLSIMDEPDFKAEFNQLLKDYVGRPSPLYFAKRLSEKYNTKVYLKRED
LNHTGAHKVNNTIGQILLAKRLGKKRIIAETGAGQHGVATATVCALMGIECIVYMGEIDIARQAPNVARMKMLGAEVRPA
LSGSRTLKDATNEAIRDWINNPVDTHYIIGSAIGPHPYPDMVTRFQSIISEEIKWQLKEKEGRENPDYVVACIGGGSNAA
GTYYHFLHEPQVGIIAVEAAGKGVDSGHSAATSKLGKVGVIHGCKTLLMQTPDGQITEPYSISAGLDYPGVGPLHAHLAQ
TGRGEFFSVTDDDAMNAGLQLTKLEGIIPAIESAHAFAVLDQKKFKPEDIVVISLSGRGDKDLDNYIDYFKL

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine [H]

COG id: COG0133

COG function: function code E; Tryptophan synthase beta chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family [H]

Homologues:

Organism=Escherichia coli, GI1787515, Length=386, Percent_Identity=53.3678756476684, Blast_Score=423, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6321412, Length=379, Percent_Identity=50.9234828496042, Blast_Score=392, Evalue=1e-110,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR006653
- InterPro:   IPR006654
- InterPro:   IPR023026 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 43126; Mature: 42994

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFNVNEKGYYGEFGGAYIPEMLYPNVEELRQKYLSIMDEPDFKAEFNQLLKDYVGRPSP
CCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCH
LYFAKRLSEKYNTKVYLKREDLNHTGAHKVNNTIGQILLAKRLGKKRIIAETGAGQHGVA
HHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHH
TATVCALMGIECIVYMGEIDIARQAPNVARMKMLGAEVRPALSGSRTLKDATNEAIRDWI
HHHHHHHHHHHHEEEECCCHHHHCCCCHHHHHHHHHHHCHHCCCCCHHHHHHHHHHHHHH
NNPVDTHYIIGSAIGPHPYPDMVTRFQSIISEEIKWQLKEKEGRENPDYVVACIGGGSNA
CCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCC
AGTYYHFLHEPQVGIIAVEAAGKGVDSGHSAATSKLGKVGVIHGCKTLLMQTPDGQITEP
CCCEEEEECCCCEEEEEEECCCCCCCCCCHHHHHHHCCCHHHHHHHHHEEECCCCCCCCC
YSISAGLDYPGVGPLHAHLAQTGRGEFFSVTDDDAMNAGLQLTKLEGIIPAIESAHAFAV
EEEECCCCCCCCCHHHHHHHHCCCCCEEEECCCHHHHCCEEEEHHHHHHHHHHCCCEEEE
LDQKKFKPEDIVVISLSGRGDKDLDNYIDYFKL
ECCCCCCCCCEEEEEECCCCCCCHHHHHHHHCC
>Mature Secondary Structure 
SFNVNEKGYYGEFGGAYIPEMLYPNVEELRQKYLSIMDEPDFKAEFNQLLKDYVGRPSP
CCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCH
LYFAKRLSEKYNTKVYLKREDLNHTGAHKVNNTIGQILLAKRLGKKRIIAETGAGQHGVA
HHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHH
TATVCALMGIECIVYMGEIDIARQAPNVARMKMLGAEVRPALSGSRTLKDATNEAIRDWI
HHHHHHHHHHHHEEEECCCHHHHCCCCHHHHHHHHHHHCHHCCCCCHHHHHHHHHHHHHH
NNPVDTHYIIGSAIGPHPYPDMVTRFQSIISEEIKWQLKEKEGRENPDYVVACIGGGSNA
CCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCC
AGTYYHFLHEPQVGIIAVEAAGKGVDSGHSAATSKLGKVGVIHGCKTLLMQTPDGQITEP
CCCEEEEECCCCEEEEEEECCCCCCCCCCHHHHHHHCCCHHHHHHHHHEEECCCCCCCCC
YSISAGLDYPGVGPLHAHLAQTGRGEFFSVTDDDAMNAGLQLTKLEGIIPAIESAHAFAV
EEEECCCCCCCCCHHHHHHHHCCCCCEEEECCCHHHHCCEEEEHHHHHHHHHHCCCEEEE
LDQKKFKPEDIVVISLSGRGDKDLDNYIDYFKL
ECCCCCCCCCEEEEEECCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12663928 [H]