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The map label for this gene is phaA [H]

Identifier: 146302339

GI number: 146302339

Start: 5576482

End: 5577660

Strand: Direct

Name: phaA [H]

Synonym: Fjoh_4612

Alternate gene names: 146302339

Gene position: 5576482-5577660 (Clockwise)

Preceding gene: 146302336

Following gene: 146302340

Centisome position: 91.46

GC content: 41.14

Gene sequence:

>1179_bases
ATGAACAAAAGAGTTGTTATCGTTTCTGCCGTTAGAACACCTATCGGAAGTTTCATGGGCGGGTTATCTAGTGTACCTGC
ACCAAAATTAGGAGCTGCTGCCATAAAAGGAGCGCTTTCAAAAATTAATCTTGACCCAAAATTAGTCGATGAAGTTTTCA
TGGGGAATGTAATTCAGGCCGGAGTTGGACAAGCGCCAGCGCGTCAGGCTGCATTATTTGCCGGATTATCTGAAGAAGTT
GCTGCCACAACAGTAAACAAAGTATGTGCATCCGGAATGAAAGCTGTAATGTTTGCTGCTCAGGCAATCGCATGCGGTGA
CGCTGAAATTGTAGTTGCGGGCGGAATGGAAAGCATGAGCTTGATTCCTCATTACATACAAATGCGTGCCGGAAACAAAT
TTGGTCCTGCAACTATGCTTGACGGAATGCAGAAAGACGGTTTAACAGATGCTTACGACAACAACGCAATGGGAGTTTGC
GCTGATTTATGTGCATCTGAATACAAAATCAGCCGTGAAAAGCAAGATGCATTCGCAATTCAATCGTATGAAAGAAGTGC
AAAAGCCTGGGATGCCGGAAAATTCGACAATGAAGTTGTACCTGTTGAAGTACCTCAGAGACGCGGTGAACCCATTATAT
TTTCAAAAGATGAAGAATATACTAATGTAAAATTAGATAAAATTCCATCTTTAGCCCCAGTTTTCACAAAAGACGGAACT
GTAACTGCTGCAAATGCTTCAACAATCAACGACGGAGCAGCTGCTTTAGTTTTAATGTCTGAAGAAAAAGCAAATGCATT
AGGTTTAAAACCTTTAGCTTACATAAAAGGCTACGCAGATGCTGCACAAGAACCAAAATGGTTTACAACAAGTCCGGCAA
AAGCATTACCAAAAGCTTTAAACAAAGCAGGAATTTCAATCTCAGATGTTGATTATTTCGAATTCAACGAAGCTTTCTCT
GTAGTTGGATTAGCCAATGCAAAAATATTAAACCTTGATAACGATAAAGTAAACGTAAACGGTGGTGCTGTTTCATTAGG
ACATCCGCTTGGAGCATCCGGAGCACGTATTATTGTAACTTTACTAAATGTTTTAGAACAAAACAATGCAAAAACCGGAG
CTGCTGCAATTTGCAACGGCGGCGGTGGCGCATCGGCAATTGTTATCGAAAGAGCTTAA

Upstream 100 bases:

>100_bases
AAAAATTTCACCTTAAATTTAAAATTCACCTATTCAATACAAAATTTGAATTTATTCAAATAAACAGTAAATTTGCTTAC
TAACTATTATTAAACAGAAA

Downstream 100 bases:

>100_bases
AACAATATCATAAAAAATCAGGAGTTATATGTATAACTCCTGATTTTCAACTAATACTTTCCCTTAAATGTTTGGAATTT
GTAATCTAGCCATAGTACCC

Product: acetyl-CoA acetyltransferase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MNKRVVIVSAVRTPIGSFMGGLSSVPAPKLGAAAIKGALSKINLDPKLVDEVFMGNVIQAGVGQAPARQAALFAGLSEEV
AATTVNKVCASGMKAVMFAAQAIACGDAEIVVAGGMESMSLIPHYIQMRAGNKFGPATMLDGMQKDGLTDAYDNNAMGVC
ADLCASEYKISREKQDAFAIQSYERSAKAWDAGKFDNEVVPVEVPQRRGEPIIFSKDEEYTNVKLDKIPSLAPVFTKDGT
VTAANASTINDGAAALVLMSEEKANALGLKPLAYIKGYADAAQEPKWFTTSPAKALPKALNKAGISISDVDYFEFNEAFS
VVGLANAKILNLDNDKVNVNGGAVSLGHPLGASGARIIVTLLNVLEQNNAKTGAAAICNGGGGASAIVIERA

Sequences:

>Translated_392_residues
MNKRVVIVSAVRTPIGSFMGGLSSVPAPKLGAAAIKGALSKINLDPKLVDEVFMGNVIQAGVGQAPARQAALFAGLSEEV
AATTVNKVCASGMKAVMFAAQAIACGDAEIVVAGGMESMSLIPHYIQMRAGNKFGPATMLDGMQKDGLTDAYDNNAMGVC
ADLCASEYKISREKQDAFAIQSYERSAKAWDAGKFDNEVVPVEVPQRRGEPIIFSKDEEYTNVKLDKIPSLAPVFTKDGT
VTAANASTINDGAAALVLMSEEKANALGLKPLAYIKGYADAAQEPKWFTTSPAKALPKALNKAGISISDVDYFEFNEAFS
VVGLANAKILNLDNDKVNVNGGAVSLGHPLGASGARIIVTLLNVLEQNNAKTGAAAICNGGGGASAIVIERA
>Mature_392_residues
MNKRVVIVSAVRTPIGSFMGGLSSVPAPKLGAAAIKGALSKINLDPKLVDEVFMGNVIQAGVGQAPARQAALFAGLSEEV
AATTVNKVCASGMKAVMFAAQAIACGDAEIVVAGGMESMSLIPHYIQMRAGNKFGPATMLDGMQKDGLTDAYDNNAMGVC
ADLCASEYKISREKQDAFAIQSYERSAKAWDAGKFDNEVVPVEVPQRRGEPIIFSKDEEYTNVKLDKIPSLAPVFTKDGT
VTAANASTINDGAAALVLMSEEKANALGLKPLAYIKGYADAAQEPKWFTTSPAKALPKALNKAGISISDVDYFEFNEAFS
VVGLANAKILNLDNDKVNVNGGAVSLGHPLGASGARIIVTLLNVLEQNNAKTGAAAICNGGGGASAIVIERA

Specific function: Involved in the production of polyhydroxyalkonic acids (PHAs), composed primarily of 3-hydroxybutyric acid (3HB) and 3- hydroxyvaleric acid (3HV) [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4557237, Length=391, Percent_Identity=53.9641943734015, Blast_Score=398, Evalue=1e-111,
Organism=Homo sapiens, GI148539872, Length=391, Percent_Identity=47.3145780051151, Blast_Score=335, Evalue=3e-92,
Organism=Homo sapiens, GI167614485, Length=388, Percent_Identity=39.4329896907216, Blast_Score=269, Evalue=3e-72,
Organism=Homo sapiens, GI4501853, Length=393, Percent_Identity=38.1679389312977, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI4504327, Length=433, Percent_Identity=35.3348729792148, Blast_Score=216, Evalue=3e-56,
Organism=Homo sapiens, GI194353979, Length=389, Percent_Identity=30.0771208226221, Blast_Score=132, Evalue=7e-31,
Organism=Escherichia coli, GI1788554, Length=392, Percent_Identity=50.5102040816327, Blast_Score=340, Evalue=8e-95,
Organism=Escherichia coli, GI87082165, Length=391, Percent_Identity=44.7570332480818, Blast_Score=335, Evalue=2e-93,
Organism=Escherichia coli, GI1787663, Length=400, Percent_Identity=38.5, Blast_Score=251, Evalue=5e-68,
Organism=Escherichia coli, GI48994986, Length=397, Percent_Identity=38.5390428211587, Blast_Score=231, Evalue=9e-62,
Organism=Escherichia coli, GI1788683, Length=426, Percent_Identity=31.6901408450704, Blast_Score=188, Evalue=5e-49,
Organism=Caenorhabditis elegans, GI25147385, Length=390, Percent_Identity=53.0769230769231, Blast_Score=391, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17535921, Length=394, Percent_Identity=44.4162436548223, Blast_Score=313, Evalue=1e-85,
Organism=Caenorhabditis elegans, GI133906874, Length=391, Percent_Identity=40.920716112532, Blast_Score=276, Evalue=9e-75,
Organism=Caenorhabditis elegans, GI17535917, Length=397, Percent_Identity=40.5541561712846, Blast_Score=246, Evalue=1e-65,
Organism=Caenorhabditis elegans, GI17551802, Length=425, Percent_Identity=34.8235294117647, Blast_Score=202, Evalue=4e-52,
Organism=Saccharomyces cerevisiae, GI6325229, Length=397, Percent_Identity=59.1939546599496, Blast_Score=445, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6322031, Length=397, Percent_Identity=33.7531486146096, Blast_Score=193, Evalue=4e-50,
Organism=Drosophila melanogaster, GI24640423, Length=388, Percent_Identity=53.3505154639175, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24655093, Length=388, Percent_Identity=48.9690721649485, Blast_Score=353, Evalue=8e-98,
Organism=Drosophila melanogaster, GI17648125, Length=396, Percent_Identity=37.3737373737374, Blast_Score=226, Evalue=3e-59,
Organism=Drosophila melanogaster, GI17137578, Length=429, Percent_Identity=34.032634032634, Blast_Score=205, Evalue=5e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 40758; Mature: 40758

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKRVVIVSAVRTPIGSFMGGLSSVPAPKLGAAAIKGALSKINLDPKLVDEVFMGNVIQA
CCCEEEEEEEHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCHHHH
GVGQAPARQAALFAGLSEEVAATTVNKVCASGMKAVMFAAQAIACGDAEIVVAGGMESMS
CCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHH
LIPHYIQMRAGNKFGPATMLDGMQKDGLTDAYDNNAMGVCADLCASEYKISREKQDAFAI
HHHHHHHHHCCCCCCCHHHHCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHH
QSYERSAKAWDAGKFDNEVVPVEVPQRRGEPIIFSKDEEYTNVKLDKIPSLAPVFTKDGT
HHHHHHHCCCCCCCCCCCEEEEECCCCCCCCEEEECCCCCCCEEHHCCCCCCCEEECCCE
VTAANASTINDGAAALVLMSEEKANALGLKPLAYIKGYADAAQEPKWFTTSPAKALPKAL
EEECCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCEECCHHHHHHHHH
NKAGISISDVDYFEFNEAFSVVGLANAKILNLDNDKVNVNGGAVSLGHPLGASGARIIVT
HHCCCEEECCCHHHHHCHHEEEEECCEEEEECCCCEEEECCCEEEECCCCCCCCHHHHHH
LLNVLEQNNAKTGAAAICNGGGGASAIVIERA
HHHHHHCCCCCCCCEEEECCCCCCEEEEEECC
>Mature Secondary Structure
MNKRVVIVSAVRTPIGSFMGGLSSVPAPKLGAAAIKGALSKINLDPKLVDEVFMGNVIQA
CCCEEEEEEEHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCHHHH
GVGQAPARQAALFAGLSEEVAATTVNKVCASGMKAVMFAAQAIACGDAEIVVAGGMESMS
CCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHH
LIPHYIQMRAGNKFGPATMLDGMQKDGLTDAYDNNAMGVCADLCASEYKISREKQDAFAI
HHHHHHHHHCCCCCCCHHHHCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHH
QSYERSAKAWDAGKFDNEVVPVEVPQRRGEPIIFSKDEEYTNVKLDKIPSLAPVFTKDGT
HHHHHHHCCCCCCCCCCCEEEEECCCCCCCCEEEECCCCCCCEEHHCCCCCCCEEECCCE
VTAANASTINDGAAALVLMSEEKANALGLKPLAYIKGYADAAQEPKWFTTSPAKALPKAL
EEECCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCEECCHHHHHHHHH
NKAGISISDVDYFEFNEAFSVVGLANAKILNLDNDKVNVNGGAVSLGHPLGASGARIIVT
HHCCCEEECCCHHHHHCHHEEEEECCEEEEECCCCEEEECCCEEEECCCCCCCCHHHHHH
LLNVLEQNNAKTGAAAICNGGGGASAIVIERA
HHHHHHCCCCCCCCEEEECCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14500782; 10427049 [H]